3€ creating and saving data using core data

... buffer into an NSData */ NSData *readData = [[NSData alloc] initWithBytes:(const void *)buffer length:bytesRead]; /* And write the data to file */ [fileHandle writeData:readData]; } while (bytesRead ... a certain table, and so on (Actually, Core Data doesn’t use the concept of a “table,” but I’m using the term here because it’s familiar and will help you understand how Core Data works.) Managed ... 649 Figure 13-1 Creating an empty application for Core Data Now for your product name choose Introduction to Core Data and make sure the User Core Data check box is ticked, as shown in Figure 13-2...

Ngày tải lên: 13/08/2014, 18:20

... that handle graphics and animations in iOS and OS X Some of these frameworks and technologies are: UIKit The high-level framework that allows developers to create views, windows, buttons, and other ... delete, and modify) events in the Calendar database from inside your application, using built-in SDK view controllers Solution Instantiate an object of type EKEventEditViewController and present ... 15: Graphics and Animations Figure 15-4 Saving our Xcode project on disk On the right side, select Objective-C class, and then press Next (see Figure 15-5) 15.0 Introduction | 739 Figure 15-5 Creating...

Ngày tải lên: 13/08/2014, 18:20

... template DNA using oligonucleotides H623 and H625 and Pfu high®delity polymerase (Stratagene, La Jolla, CA, USA) The single ampli®cation product obtained was digested with SphI and BglII, and ligated ... respective strains KM10 and KM11 Correct integration of these plasmid fragments was veri®ed by polymerase chain reaction using oligonucleotide pairs H338 and H162, or H339 and H161, respectively ... chromatography using a Ni2+-containing Sepharose 6B column (Pharmacia), and protein was puri®ed to near homogeneity using a Ni-nitrilotriacetic acid Spin Kit (Qiagen) SDS/PAGE revealed a protein band with...

Ngày tải lên: 22/02/2014, 04:20

... human DIDO isoforms and were searched against PFAM [22,23] and SMART [24] databases to automatically detect domains; they were further used as queries against NCBI databases using PSIBLAST [25,26] ... cultures from targeted (PHD and dPHD regions) and wild-type mice were fixed with methanol and acetic acid, mounted in Vectashield containing DAPI, and studied by standard fluorescence microscopy ... significant e-values of 2e-30, 1e-10 and 1e-08 for DATF-1, PHF3 and CGBP, respectively Given the broad distribution and functional repertoire for the PHD domain in the databases, we studied its evolution...

Ngày tải lên: 07/03/2014, 21:20

... further recruits Pol b and XRCC1–DNA ligase IIIa to accomplish repair Among the known DSPs are APE1 and PNK which recognize and process 3¢ ends containing phosphoglycolate and phosphate groups, ... manufacturer Mammalian DNA single-strand break repair EDTA, 17% glycerol and mm dithiothreitol Extracts were divided into aliquots and stored at )80 °C Immunodepletion of WCEs and western blots Immunodepletion ... described [36] and verified by SDS ⁄ PAGE (10% gel) Proteins were transferred to poly(vinylidene difluoride) membranes and immunoblotted using the corresponding antibodies Blots were visualized using the...

Ngày tải lên: 23/03/2014, 11:20

... [19,20]/TOCSY [19–21] and DQF-COSY [19,20,22] spectra, respectively NOESY and TOCSY spectra were acquired at 50, 100, 150 and 200 ms mixing times with 56 scans per each t1 increment, and 50, 75 and 150 ms ... Cys47-Cys58 and Cys59-Cy64, and are involved in a double- and a triple-stranded b-sheet, i.e the main regular secondary structures of NTX-1 Loop I (residues 1–15) constitutes a double-stranded antiparallel ... > Hz and )60° ± 30° for 3JNHa < Hz Results and Discussion Resonance assignment NMR assignments were carried out according to standard methods [32] Identification of spin systems was made using...

Ngày tải lên: 23/03/2014, 13:20

... vaccination against HIV has expanded and resulted in hundreds of preclinical and clinical studies Interestingly, the most promising clinical regimens to date include: i) the RV144 landmark HIV ‘Thai trial’ ... prime-boost vaccines and potentially optimizing homologous prime-boost regimens, applicable to difficult and unmet indications such as cancer and chronic viral infections The core principle of this ... modifiers by direct intra-nodal administration of plasmid and peptide [19,41] We showed that the sequence and the route of administration of plasmid and peptide were absolutely essential to achieve improved...

Ngày tải lên: 18/06/2014, 16:20

... skills will be the ability to win friends and influence people at a personal level, the ability to structure partnerships, and the ability to negotiate and to find compromises Business will be ... chapters Ride the Ride  Negotiations are as varied as roller-coasters  How to survive and prosper – using strategies, tools & techniques will be explored in this class Changing Nature of Business ... Business will be much more about finding the right people in the right places and negotiating the right deals.” Charles Handy 2002 Negotiation Defined  Process involving interested parties who •...

Ngày tải lên: 31/07/2014, 17:20

... isolation and western blotting Nuclear and cytoplasmic extracts were isolated using a modified method of Dignam and colleagues [20], as previously described [10] Total cell extracts were isolated using ... to 18S and normalized to vehicletreated cells Data were log-transformed prior to analysis by one-way analysis of variance and Tukey's post-hoc test, paired t tests and Student's t tests, using ... Agc1, Hapln1, and Col2a1, proteases Mmp-9 and Mmp-12, as well as the inflammatory cytokine macrophage Csf-1 (Figure 3) TNF decreased Agc1 and Hapln1 (Figure 3a, b) and increased Mmp-9 and Mmp-12...

Ngày tải lên: 09/08/2014, 01:22

... IL-1β, IL-1Ra, and IL-1RI), matrix-degrading enzymes (MMP-3, MMP-13, ADAMTS-4, and ADAMTS-5), matrix proteins (aggrecan and collagen types I and II), and two SOX genes (6 and 9) Primers and Probes ... of this Averages and standard deviations were calculated for disc sections grouped with the scores to 3, to 6, to 9, and 10 to 12 Data was then presented on graphs as means ± standard errors to ... work and analysis, and drafted the manuscript AJF conceived the study and participated in its design and coordination JAH conceived the study, participated in its design and coordination, and...

Ngày tải lên: 09/08/2014, 06:22

... sequence detection system) using specific primers and probes (Table 2) The reaction was conducted as follows: 95°C for min, and 40 cycles of 15s at 95°C and at 60°C (21) To standardize mRNA levels, ... sequence alignment, and drafted the manuscript HN, K H-M, TK, and KN conceived the study, participated in its design and coordination, and helped to draft the manuscript All authors read and approved ... solution and then decalcified in 4% paraformaldehyde containing 0.85% sodium chloride and 10% acetic acid Tissues were dehydrated in a series of ethanol solutions and infiltrated with xylene and before...

Ngày tải lên: 09/08/2014, 06:23

... Netherlands), dissolved in dimethylsulfoxide or methanol and used as controls The proteasome inhibitor PSI and the JNK inhibitor SP600125 were purchased at Omnilabo (Breda, The Netherlands) and ... binding sites was performed using the GEMSLauncher version 3.6 from Genomatrix and MatInspector professional release [22] Core and matrix similarity settings were 0.75 and optimized -0.03, respectively ... in Materials and methods Data are expressed as mean ± standard error of the mean and are representative of four independent experiments *P < 0.0005 7α-OH-DHEA = 7α-hydroxy-dehydroepiandrosterone...

Ngày tải lên: 09/08/2014, 07:20

Ngày tải lên: 09/08/2014, 07:20

... Brugger LE, Candinas D: Negative appendicectomy and perforation rates in patients undergoing laparoscopic surgery for suspected appendicitis Br J Surg 2011 Sadr Azodi O, Andren-Sandberg A, Larsson ... A, Larsson H: Genetic and environmental influences on the risk of acute appendicitis in twins Br J Surg 2009, 96(11):1336-1340 Andren-Sandberg A, Korner H: Quantitative and qualitative aspects ... aetiology and pathogenesis which may eventually help us understand this disease better Finally, a drive towards nonoperative management of “non-complicated” appendicitis has been advocated through randomised...

Ngày tải lên: 13/08/2014, 23:20

... Eleni, Alexandros and Pavlos Michaelidou; Antonio Colangelo and Mariagrazia Mecoli I thank them for being on my side all these years, for believing in me and for making my life in Tucson fun and enjoyable ... Georgia Papageorgiou and Anna Sidera for their continual support and the great summers I spent with them I thank my grandparents Panagiota and Charalambos Konstantinou, Maria Zeniou and my spiritual ... guidance, and advice, his continual encouragement and incredible patience, and for caring, during the years of my doctoral work I want to thank him especially, for being such an inspiration to me, and...

Ngày tải lên: 13/11/2014, 09:14

... single-stranded 3’ overhang of the G-rich strand (A) Specific protein complexes bind to the double- and single-stranded telomeric DNA (B) The single-stranded overhang can invade the double-stranded ... contains a leading and a lagging strand Lagging strand synthesis cannot be completed because the removal of primers thus causing net loss of DNA base pairs from the lagging strand (Hug and Lingner, ... cancer and aging Singapore • Balakrishnan L, Gurung RL, Hande MP (2007) Effect of Combined inhibition of PARP-1 and telomerase 5th Anniversary Congress of International Drug Discovery Science and...

Ngày tải lên: 11/09/2015, 10:18

... from the wells and washed times with wash buffer Standards and samples were then added to the wells with appropriate dilutions and incubated at room temperature for hours Standards and samples were ... JNK (Cargnello and Roux, 2011) JNK and of 46 and 52 kDa respectively exhibit vast tissue distribution while JNK 3, is localized to neural tissues, testis and cardiac myocytes (Bode and Dong, 2007) ... which precedes the core C-terminal domain, is unique 27 among the annexins and therefore determines its vast biological properties and functions (Raynal and Pollard, 1994; Rescher and Gerke, 2004)...

Ngày tải lên: 01/10/2015, 17:27

... tissue, and remove degenerating and dysfunctional cells (Harry and Kraft, 2008) The activated microglia in response to CNS inflammation secrete proinflammatory cytokines such as TNF-α and IL-1β and ... Carson and Sutcliffe, 1999, Frei and Fontana, 1997, Hickey and Kimura, 1988) Increases in TNF-α and IL-1β have been observed prior to neuronal death (Harry, et al., 2008, Lefebvre d'Hellencourt and ... important for growth and survival, whereas sphingosine and ceramide are associated with cell growth arrest and apoptosis (Ogretmen and Hannun, 2004) Therefore the balance between ceramide and sphingosine...

Ngày tải lên: 16/10/2015, 15:38

... the project as it currently stands and its future direction They also put the project’s progress into the context of where it stands compared to its original scope and objective because they holistically ... • Decisions reached and make by the project team • Activities and tasks accomplished • Milestones reached • Work still to be done • Any issues or problems that have arisen and need to be addressed ... materials, hire of equipment and administrative support) This will help in the management of spending & saving money D) Progress report: Regular progress reports should be prepared and should outline:...

Ngày tải lên: 24/04/2016, 12:10