3 osteogenic protein 1 in aged chondrocytes

... 58 Male 60 Female 10 54 Female 11 60 Female 12 75 Female 13 81 Female 14 41 Male 15 75 Female 16 67 Female 17 64 Female 18 87 Female 19 71 Male 20 70 Female 21 53 Female 22 71 Male 23 82 Male ... Osteogenic protein- 1 (OP -1) expression and processing in Chinese hamster ovary cells: isolation of a soluble complex containing the mature and pro-domains of OP -1 Growth Factors 19 94, 11 : 215 -225 ... performed (1: 1000, 1: 100, 1: 10, and 1: 5, Figure 1a,b) A 1/ 100 dilution of the SF samples yielded the clearest results The small amount of the 18 kDa band (Figure 1b) suggests that OP -1 in asymptomatic...

Ngày tải lên: 09/08/2014, 08:22

... 76 3 .1 PRAP1 and intestinal differentiation 3 .1. 1 PRAP1 is expressed in epithelial cells of the intestines The expression of PRAP1 in human intestinal tract was studied using immunohistochemistry ... 77 Figure 3 .11 PRAP1 is expressed in the epithelial cells of small intestine Representative figure showing PRAP1 immunohistochemical staining in normal human small intestine tissue (X100 magnification) ... hypothesized that PRAP1 may play a role in protecting the intestinal epithelium from invading microorganisms To verify this hypothesis, we first examined whether PRAP1 protein binds to bacteria We...

Ngày tải lên: 11/09/2015, 16:05

... Dawid IB (19 90) The trithorax gene, a trans-acting regulator of the bithorax complex in Drosophila, encodes a protein with zinc-binding domains Proc Natl Acad Sci USA 87, 211 2– 211 6 14 Tripoulas ... death inducer obliterator protein (DIDO), PHF3 and yeast proteins Additional proteins were included Naming conventions are as in Fig S1 The solid purple box indicates the TFS2M domain Pink-boxed ... found a set of DIDO1 homologues in the context of DNA binding and chromatin regulation These homologues include members of the CpG binding protein (CGBP) family of proteins that bind DNA at unmethylated...

Ngày tải lên: 07/03/2014, 21:20

... activated AP -1 [11 13 ] AP -1 is a complex composed of the Fos and Jun proteins [14 16 ] In general, Fos and Jun family proteins function as dimeric transcription factors that bind to AP -1 regulatory ... assays (EMSAs) using radiolabeled oligonucleotides containing either a STAT5B-binding site or an AP -1- binding site in the rat AGT gene promoter As shown in Fig 2A,B, DNA protein complexes were ... compilation ª 2009 FEBS No antibody Input 17 23 STAT5B and AP -1 interaction in AGT gene activation A M Han et al +1 AP -1 STAT5 tataaa –282~–277 17 2~ 16 3 TATA AP -1 – 419 ~– 412 B Relative luciferase activity...

Ngày tải lên: 30/03/2014, 02:20

... 2003, 9: 11 80 -11 86 41 Wellen KE, Hotamisligil GS: Inflammation, stress, and diabetes J Clin Invest 2005, 11 5: 11 11- 111 9 25 Figure Legends Figure MCPIP1 mRNA and protein levels are elevated in mouse ... response in the ischemic area This study seeks to determine if monocyte chemotactic protein induced protein (MCPIP1), a recently identified CCCH Zn finger-containing protein, plays a role in focal ... elevated in the mouse brain by LPS preconditioning treatments MCPIP1 induction in Mouse Brain Cortex after MCAO We determined whether MCPIP1 is induced by brain ischemia stress We examined MCPIP1 mRNA...

Ngày tải lên: 19/06/2014, 22:20

... Wortmannin – PD98059 CONTROL ET -1 (10 nM) SB20 219 0 chondrocytes induced protein kinase inhibitors and LY83583 on endothelin -1 (ET -1) Effect of MMP -13 and MMP -1 production by human osteoarthritis induced ... J: Interleukin-1beta-converting enzyme/caspase -1 in human osteoarthritic tissues: localization and role in the maturation of interleukin-1beta and interleukin -18 Arthritis Rheum 19 99, 42 :15 77 -15 87 ... and in the presence of ET -1, using various inhibitors: µM SB 20 219 0 (inhibitor of p38 mitogen-activated protein [MAP] kinase), 10 µM PD 98059 (a selective mitogen-activated protein kinase kinase...

Ngày tải lên: 09/08/2014, 06:22

... expression by an integrin-linked kinase, which was an ankyrin repeat containing a serine/threonine protein kinase that interacted with the cytoplasmic domain of 1 integrin [ 51] Furthermore, Liu ... crystals induce matrix metalloproteinase1 through the Ras/Mitogen-activated protein kinase/c-fos/ AP -1/ metalloproteinase -1 pathway Involvement of transcription factor binding sites AP -1 and PEA-3 ... Rheum 19 93, 36 :12 74 -12 85 Forsyth CB, Pulai J, Loeser RF: Fibronectin fragments and blocking antibodies to α2 1 and α5 1 integrins stimulate mitogen-activated protein kinase signaling and increase...

Ngày tải lên: 09/08/2014, 06:23

... stress-induced HIFR 911 1 expression in chondrocytes in hypoxia Local accumulation of a regulating protein to adapt to hypoxia may be mediated, at least in part, by p38 and PI3K in articular chondrocytes ... they provide new insights into pathogenesis of OA 14 15 16 17 Competing interests The authors declare that they have no competing interests 18 Authors' contributions KY carried out in vitro studies ... catabolic activity, including production of metalloproteinases [ 31, 32] IL -1 also stimulates chondrocyte expression of inducible nitric oxide synthesis, iNOS, which results in an increase in NO production...

Ngày tải lên: 09/08/2014, 06:23

... AP -1 within the Cyp7b promoter are in accordance with the findings in this report that NF-κB and AP -1 are involved in the TNF-α-enhanced Cyp7b activity Figure NF-κB and AP -1 binding sites within ... 465:23-27 29 Johnson GL, Lapadat R: Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases Science 2002, 298 :19 11- 1 912 R1280 ... (NF-κB) [12 ] Three MAPK families have been implicated to play a role in RA, including extracellular signal (mitogenic)-regulated protein kinase (ERK )1/ 2; the stress-activated protein kinases,...

Ngày tải lên: 09/08/2014, 07:20

Ngày tải lên: 09/08/2014, 07:20

... are involved in IL1-induced downregulation of PPAR 1 IL -1 is known to induce its effects in chondrocytes through activation of a plethora of signaling pathways, including the mitogen-activated protein ... in joints, including chondrocytes [11 ,13 ] Natural and synthetic ligands of PPARγ were shown to inhibit the expression of several inflammatory and catabolic genes in cultured chondrocytes [4 ,11 ,12 ] ... activators inhibit interleukin -1 -induced nitric oxide and matrix metalloproteinase 13 production in human chondrocytes Arthritis Rheum 20 01, 44:595-607 12 Fahmi H, Pelletier JP, Mineau F, Martel-Pelletier...

Ngày tải lên: 09/08/2014, 10:20

... rhOP -1 B A 99 11 0 83 8 310 1 86 10 1 14 4 10 8 17 3 416 13 7 Binding Biological Process 16 1 418 13 0 93 10 3 Cellular Component 16 1 10 0 Cellular Process Molecular Function Genes up-regulated by OP-1AS ... [GenBank:NM_0 015 61] 1. 69↓ [GenBank:NM_0 012 50] [GenBank:NM_003808] TNF-a induced protein TNF-a induced protein- 3 2.02↓ TNF-R12 1. 79↓ TNF-R9 1. 73↓ TNF-R5 TNF -13 [GenBank:NM_ 016 639] 1. 88↑ IL1-R1 1. 59↓ [GenBank:NM_000877] ... receptor) 1. 60↑ 1. 70↓ 1. 60↓ 1. 60↑ SPRY2: sprouty homolog (Drosophila) 1. 60↓ 1. 60↑ SORBS1: sorbin and SH3 domain containing 1. 70↑ 1. 50↓ PIK3R1 (Phosphoinositide-3-kinase, regulatory subunit 1) 1. 72↑...

Ngày tải lên: 12/08/2014, 15:22

... spots /10 00 cells 60 40 ** ** 30 20 10 - + + + 10 10 0 10 10 0 LPS (ng/ml) IL -10 IL -1 IL-6 + 10 10 0 + 10 + 10 0 + - + 10 10 0 + 10 + 10 0 + - - - + 10 10 0 + 10 + 10 0 + + LPS (ng/ml) + 10 10 0 + 10 + 10 0 ... enzyme-linked immunospot assay; HMGB1: high mobility group box protein 1; IL -1 : interleukin alpha; IL -1 : interleukin beta; IL-1RI: interleukin receptor type 1; IL-1RA: interleukin receptor antagonist; ... Helsinki Preparation of rHMGB1 from E coli Recombinant rat HMGB1 (rHMGB1) with a 99% identity to human HMGB1 [44] and containing a calmodulin-binding protein tag was expressed in E coli strain...

Ngày tải lên: 12/08/2014, 17:22

... HMGB1 may contribute to outcome in this clinical setting Antibodies to HMGB1 improve outcome in animal models of sepsis, clearly demonstrating a role for HMGB1 in contributing to mortality in ... thereby worsening organ dysfunction Additional studies aimed at characterizing the co-factors bound to HMGB1 in the setting of severe accidental injury would be extremely useful in delineating the ... to mortality in severe infection [6 ,11 ] While initial studies postulated that the benefit from blocking HMGB1 was through inhibiting direct deleterious effects of HMGB1 on organ function, it...

Ngày tải lên: 13/08/2014, 19:20

... .xvii Chapter 1: Introduction 1. 1 Review of Literature 1. 1 .1 1 .1. 2 Inducers of genomic instability 10 1. 1.2 .1 Oxidative stress 10 1. 1.2.2 Arsenic-induced oxidative ... .14 1. 1.2.3 Radiation 18 1. 1.3 Mechanisms for preventing genomic instability 20 1. 1.3 .1 Poly (ADP-ribose) polymerase (PARP -1) 21 1 .1. 3 .1. 1 Role of PARP -1 at the telomeres 24 1. 1.4 ... 1. 1 .1. 1 .1 Telomeres 1. 1 .1. 1.2 Telomere dysfunction and tumourigenesis 1. 1 .1. 1.3 DNA repair proteins in telomere maintenance .8 Copper chaperone, Antioxidant protein (ATOX1) 31 Rationale...

Ngày tải lên: 11/09/2015, 10:18

Ngày tải lên: 11/09/2015, 16:05

... morphology 13 8 3 .11 .1. 2 Effects PRAP1 on actin filament 13 9 3 .11 .1. 3 Effects of PRAP1 on microtubules 14 1 3 .11 .2 PRAP1 interacts with Hsp 70 14 1 3 .12 Role of PRAP1 in apoptotic ... 3 .13 .4 PRAP1 expression in PBMC 16 1 3 .14 Regulation of PRAP1 expression in lymphocytes 16 5 3 .14 .1 PRAP1 is induced by PHA/PMA 16 5 3 .14 .2 PRAP1 is induced by UV 16 5 3 .14 .3 ... 2 .10 .1 Detection by ELISA 70 2 .10 .2 Detection by direct binding 71 2 .10 .2 .1 Labeling of protein with Alexa Fluor dye 71 2 .10 .2.2 Bacteria binding assay using labeled protein...

Ngày tải lên: 11/09/2015, 16:05

... an increase in cyclin E1 and cyclin A1 protein levels, reflecting an arrest at S-phase (Figure 3.55) Repression of PRAP1 induction by 5FU caused a significant reduction in cyclin A1 expression, ... elements ( +13 16 and +14 60) in PRAP1 are illustrated in Figure 3.42 In order to determine whether the two identified p53-response elements in PRAP1 (PRAP1-p53BS) are functional, we amplified the intronic ... specific inhibitors of caspase 3, and were able to block cell death induced by 5-FU in HCT 11 6 cells, indicating that 5-FU induces cell 13 4 5-FU A - + Control siRNA PRAP1 siRNA1 B % of Sub-G1 20...

Ngày tải lên: 11/09/2015, 16:05

... analysis 14 4 Figure 3.70 Identification of PRAP1 binding protein by MS-MS Representative figure showing the identification of PRAP1 binding proteins after performing MS-MS Figure 3. 71 PRAP1 physically ... resulting in a change in cellular morphology 14 0 Figure 3.67 Effects of PRAP1 on actin network Representative immunofluorescence images of F-actin in HCT 11 6 cells stably expressing PRAP1 (PRAP1) and ... resulted in cellular morphological changes 3 .11 .1. 2 Effects PRAP1 on actin filament To study the effect of PRAP1 on the organization of F-actin, an important cytoskeletal protein, we stained F-actin...

Ngày tải lên: 11/09/2015, 16:05

... DISCUSSION 17 3 4 .1 Role of PRAP1 in differentiated epithelial cells 4 .1. 1 Regulation of PRAP1 by differentiation 4 .1. 1 .1 Expression of PRAP1 in intestinal epithelium The proline-rich acidic protein (PRAP1) ... we employed the annexin V-FITC staining assay Annexin V is a phospholipid binding protein that has a high binding affinity for phosphatidylserine (PS) that translocates from inner to outer leaflet ... PRAP1 is necessary but not sufficient to induce S-phase arrest Interestingly, PRAP1 knockdown resulted in an increase in the level of p 21 This increase is mainly in the cytoplasmic pool of p21...

Ngày tải lên: 11/09/2015, 16:05