3 msc mesenchymal stem cell multipotent stromal cell

... the MSCs did not affect the proliferation rate of the cells over time 3.4.6 Differentiation potential of labeled MSC MSCs are multipotent stem cells, which can differentiate to different lineage ... labeled cells (the average amount of the iron nanoparticles per cell was assumed as 10ng /cell; e.g 1, 10, 1000 µg iron nanoparticles were mixed with agarose as representative of 100, 1000, 100000 cells) ... pig’s knee explants with blank and scaffold only defect (A), 100 cells simulation (B), 1000 cells simulation (C), and 100,000 cells simulation (D) Left panel of each image is 3D-FSE sequence...

Ngày tải lên: 10/09/2015, 09:11

... Therapeutic potential of mesenchymal stem cells producing interferonalpha in a mouse melanoma lung metastasis model, Stem Cells 26, (2008), 2332-8 Satija, N K et al., Mesenchymal stem cell- based therapy: ... cell- based therapy: a new paradigm in regenerative medicine, J Cell Mol Med 13, 2009 STEM CELL RESEARCH USING MESENCHYMAL (MESENCHYMAL STEM CELL - MSC) IN THE TREATMENT OF CANCER AGENCY - RECTUM RATS ... some characteristics of mesenchymal stem cells in cancer immunotherapy in mice Research results show that the mesenchymal stem cells injected into mice at the dose of 107 cells/ml can inhibit the...

Ngày tải lên: 20/03/2014, 09:21

... the stem cells and/or attract the endogenous or exogenous stem cells (endogenous from the bone marrow and exogenous by multiple intra-articular injection of the expanded autologous stem cells) ... mediating differentiation of bone marrowand adipose tissue-derived human mesenchymal stem cells into three mesenchymal lineages Stem Cells 2007 Mar;25(3):750-60 PubMed PMID: 17095706 Epub 2006/11/11 ... till now (281) Previous studies showed that BM MSCs and synovium derived mesenchymal stem cells (SMSCs) are promising stem cell sources for cartilage repair Although some in vitro (282, 283) and...

Ngày tải lên: 10/09/2015, 09:01

... cells Pancreatic stem cells Intraislet, nestin-positive cells, oval cells, duct cells Beta cells Skletal-muscle stem cells or satellite cells Muscle fibers Skeletal muscle fibers Stem cells of the ... iv   1.5 Types of stem cells 14   1.5.1 Embryonic stem cells (ESCs) 15   1.5.2 Induced pluripotent stem (iPS) cells 15   1.5.3 Adult stem cells 16   1.5.4 Heterologous stem cells 17   1.6 MSCs ... stem cells Adult stem cells, or tissue-specific stem cells, are isolated from different adult tissues and are considered multipotent because their differentiation capacity is limited to the cell...

Ngày tải lên: 10/09/2015, 09:10

... culture media would increase the intracellular iron content of MSCs 61   Average iron content per cell (pg /cell) 200 150 100 50 Average iron content per cell (pg /cell) 0µg/ ml 25µg/ ml 50µg/ ml 75µg/ ... cytoplasm of the labeled cells   3.4.4 Iron content quantification in labeled-MSCs As shown in figure 3.6, mean iron content in labeled cells ranged from to 141 pg per cell The results showed ... than 98% of cells were negative for hematopoietic markers (CD14, CD31, CD34, and CD45), and endothelial marker (CD31) (figure 3.3) 58     Figure 3-3 Flow cytometry analysis of the stem cells surface...

Ngày tải lên: 10/09/2015, 09:10

... labeled cell localization data (Arrowheads show the distribution of the labeled cells in the knee joint) MSCs were distributed in different places of the knee (figure 3.14) The labeled cells migrated ... site and arrows demonstrate the labeled stem cells) 72     Figure 3-16 MRI of the distribution of the labeled MSCs in the mini-pig knee joint The labeled cells migrated to the para-patellar fat ... (calculated based on Wakitani scoring system at table 3.1) were compared between the scaffold only and scaffold plus MSCs groups and showed a significant difference in the cell morphology (hyaline cartilage...

Ngày tải lên: 10/09/2015, 09:11

Ngày tải lên: 10/09/2015, 09:11

... (189) This difference could be the result of lower loading of the cells (5 pg iron per cell) by Heymer et al., which is similar to our cells iron content labeled with lower labeling concentration ... chondrogenic differentiation Farrell et al (204) showed that SPIO labeled cells negatively affected the osteogenic differentiation of the cells in vivo In contrast, our study showed that iron labeling did ... MSCs is indeed feasible in vivo Cells migration and localization into different site of the inflammation was observed in the knee joint over time A majority of the cells moved to the surgery sites...

Ngày tải lên: 10/09/2015, 09:11

... mosaicplasty, and cell based therapy (autologous chondrocyte implantation, or matrix associated cell implantation)(29) Recently, cell based therapy such as mesenchymal stem cells (MSCs) become ... biochemical factors essential in mimicking physiological conditions for cells after administration Key words: Mesenchymal stem cell (MSC), cell migration, microfluidic device, cartilage, tissue culture ... self-repair Cell based therapy is one promising approach in the treatment of damaged cartilage Bone marrow (BM) derived mesenchymal stem cell (MSC) is a good candidate because of their multipotent...

Ngày tải lên: 10/09/2015, 09:11

... adipose-derived mesenchymal stem cells (ADMSCs) After culturing for 14 days, majority of isolated adipose-derived stem cells expressing CD29 and CD90 characteristic of mesenchymal stem cells (n = ... availability of various cellular sources for experimental investigations [6,7,10-15] including bone marrow-derived mesenchymal stem cells (BMDMSCs), hematopoietic stem/ progenitor cells, and cells of embryonic ... infused mesenchymal stem cells are important to the recovery from acute kidney injury Am J Physiol Renal Physiol 2007, 292:F1626-1635 Bi B, Schmitt R, Israilova M, Nishio H, Cantley LG: Stromal cells...

Ngày tải lên: 18/06/2014, 19:20

... hMSCs converges toward embryonic stem cells Principal component analysis comparing the gene expression profiles of 3A6, embryonic stem cells (ESC), mesenchymal stem cells (MSC), and various tissues ... of mesenchymal stem cell in vitro BMC cell biology 2006, 7:14 Hung SC, Chen NJ, Hsieh SL, Li H, Ma HL, Lo WH: Isolation and characterization of size-sieved stem cells from human bone marrow Stem ... Multilineage potential of adult human mesenchymal stem cells Science (New York, NY) 1999, 284:143-147 Woodbury D, Reynolds K, Black IB: Adult bone marrow stromal stem cells express germline, ectodermal,...

Ngày tải lên: 10/08/2014, 05:21

... human mesenchymal stem cells generate osteosarcoma-like lesions in the lung Stem Cells 2007, 25:1586-1594 doi:10.1186/1423-0127-18-54 Cite this article as: Chang et al.: Mesenchymal stem cell ... H, Seuwen K: Stem cell characteristics of human trabecular bone-derived cells Bone 2002, 30:699-704 De Bari C, Dell’Accio F, Tylzanowski P, Luyten FP: Multipotent mesenchymal stem cells from adult ... Yarmush ML: Mesenchymal stem cells: mechanisms of immunomodulation and homing Cell Transplant 2010, 19:667-679 Lee PH, Kim JW, Bang OY, Ahn YH, Joo IS, Huh K: Autologous mesenchymal stem cell therapy...

Ngày tải lên: 10/08/2014, 10:20

... injury Besides HSCs, there also existed another kind of stem cells called MSCs (Mesenchymal Stem Cells), they could differentiated into stroma cells and acted as the “niche” in the micro-environment[24] ... on T cell proliferation was impaired, the related inhibitory effect on cell cycle was analyzed In a PHA-stimulating system without MSC co-culture, there were 67.3 ± 3.7% and 28.4 ± 2.9% T cells ... with fewer cells entering S phase However, T cells in G0/G1 phase and S phase remained 74.5 ± 1.2% and 22.1 ± 2.4% in the co-culture system of CML-derived MSCs (compared with co-culture system of...

Ngày tải lên: 10/08/2014, 10:21

... pericytes? Cell Stem Cell 2008, 3:229-230 da Silva ML, Caplan AI, Nardi NB: In search of the in vivo identity of mesenchymal stem cells Stem Cells 2008, 26:2287-2299 Caplan AI, Bruder SP: Mesenchymal stem ... perivascular origin for mesenchymal stem cells in multiple human organs Cell Stem Cell 2008, 3:301-313 Delorme B, Chateauvieux S, Charbord P: The concept of mesenchymal stem cells Regen Med 2006, ... Transplantation of bone marrow stromal cells for treatment of central nervous system diseases Adv Exp Med Biol 2006, 585:49-64 10 Le Blanc K, Ringden O: Immunomodulation by mesenchymal stem cells and clinical...

Ngày tải lên: 11/08/2014, 03:20

... multipotent mesenchymal stem cells from the bone marrow of rhesus macaques Stem Cells Dev 2005, 14:440-451 doi:10.1186/1742-4690-8-3 Cite this article as: Nazari-Shafti et al.: Mesenchymal stem cell ... Human adipose tissue is a source of multipotent stem cells Mol Biol Cell 2002, 13:4279-4295 Woodbury D, Reynolds K, Black IB: Adult bone marrow stromal stem cells express germline, ectodermal, ... used to plot the graphs Abbreviations MSCs: mesenchymal stem cells; ASCs: adipose tissue derived mesenchymal stem cells; HD: hematopoietic differentiated cells; AIDS: acquired immune deficiency syndrome;...

Ngày tải lên: 13/08/2014, 01:20

... aggregation of chondro-progenitor mesenchymal cells into precartilage condensations (Fig 5) Cellular condensation is dependent upon signals initiated by cell cell and cell matrix interactions and ... approaches encourage the investigation on stem cells with chondrogenic potential for articular cartilage repair Both pluripotent and multipotent stem cells have been reported for studying cartilage ... outcome from BMSCs treatment Pluripotent cells utilized in cartilage tissue engineering include embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) (Medvedev et al., 2010,Oldershaw...

Ngày tải lên: 09/09/2015, 08:12

... Lai, W Wong, Y Y Dan, S K Lim and H K Ho, Mesenchymal stem cell- derived exosomes promote hepatic regeneration in drug-induced liver injury models, Stem Cell Research and Therapy, 5(3):76 (2014) ... APAP and H2O2-induced on cell death in TAMH cells 112 4.3.2 Effect of lower dosage of -T3 and -T3 against APAP and H2O2-induced injury on cell viability in TAMH cells 118 4.3.3 Effect ... tocotrienol analogs in TAMH cells 104 4.2.1 Cytotoxicity of T3 analogs in TAMH cells 105 4.2.2 Cellular uptake of different concentration of T3 analogs in TAMH cells 107 4.2.3 Summary...

Ngày tải lên: 09/09/2015, 11:21

... of implants Mesenchymal stem cells (MSCs) MSCs are a population of multipotent, heterogeneous mesenchymal stromal cells [69] These cells can proliferate in vitro as plastic-adherent cells MSCs ... marrowderived mesenchymal stem cells and osteoblast differentiation on titanium with surface-grafted chitosan and immobilized bone morphogenetic protein-2, 2nd International Congress on Stem Cells and ... substrates Culture of bone marrow-derived stromal cells (BMMSC) 57 Culture of bone chip-derived osteoblasts (BC-OB) 58 Culture of human mesenchymal stem cells (hMSC) 59 (Lonza, USA) Culture of...

Ngày tải lên: 14/09/2015, 08:37