3 bone regeneration and reformation

... (www.springer.com) Preface A Brief Outline of the Aims and Target Audience of Liver Stem Cells The role of a putative stem cells and liver-specic stem cell in regeneration and carcinogenesis is reviewed in this ... standard on this topic and will be widely distributed and read by people in many scientic elds, such as cellular biology, molecular biology, tissue engineering, liver biology, cancer biology, and ... to acute liver failure and transplanting stem cells that can expand in vivo and repair the cirrhotic liver would be ideal In animal studies, both primary hepatocytes (1) and stem cell-derived...

Ngày tải lên: 29/05/2014, 17:06

... NP-40, and 20% glycerol, supplemented with protease inhibitors (1 lgÆmL)1 leupeptin and pepstatin, lgÆmL)1 aprotinin, and mm phenylmethanesulfonyl fluoride) and phosphatase inhibitors (10 mm NaF and ... China (nos 2006CB504100 and 2006CB943703), the Nature and Sciences Foundation of China (no 30393130), the Hi-tech Research and Development Program of China (no 2006AA02A101) and Grant of Beijing ... dissociated into single cells and planted in four-well plates at a density of · 104 cells per well, and then cultured under different O2 concentrations (20% O2, 10% O2, and 3% O2) for days The number...

Ngày tải lên: 30/03/2014, 04:20

... contributions NHR and NAM designed experiments, interpreted data and conceptualized manuscript RH, JK, KWA, CAS, BM, ANP, MPM, LS, DTA, and TEI provided detailed ideas and discussions, and/ or writing ... ARDS and is associated with acute systemic inflammation and vascular damage Septic patients have elevated levels of injury associated signals and EPC mobilizers such as HMGB1 [63], SDF-1 [64], and ... into endothelial cells in vitro and in vivo based on expression of CD31, eNOS, and E-selectin [31] These studies were expanded into hindlimb ischemia in mouse and rabbit models in which increased...

Ngày tải lên: 18/06/2014, 15:20

... interpreted data and conceptualized manuscript RH, JAK, JK, KWA, CAS, BM, ANP, MPM, LS, FR, and TEI provided detailed ideas and discussions, and/ or writing of the manuscript NAM and JAJ performed ... ATP in growth factor stimulated and not stimulated cells was calculated and compared for different periods before and after intervention Stem-Kine mobilizes CD34 and CD133 Cells Statistics Differences ... supplementation and days 1, 2, 7, and 14 The ratio of the average ATP was increased after 24 hrs of supplementation from a pre-treatment level of 2.13 ± 0.0.44 to 2.57 ± 0.47 (p = 0.02) After 48 hrs and...

Ngày tải lên: 18/06/2014, 16:20

... Inc., Cleveland, Ohio, USA) to be used in this study is isolated from a single bone marrow aspirate and cultured with heat inactivated fetal bovine serum (FBS) and growth factors EGF and PDGF Cells ... cells, including MAPCs and MSCs, have been shown to beneficially modulate the anti-donor immune response in organ transplantation and to promote tissue regeneration in vitro and in vivo [26-29,53] ... FCP, and HJS BF developed essential study documents EKG, PS, and PP supported the design of the study with their knowledge and experience MHD is the principal investigator of the study and the...

Ngày tải lên: 18/06/2014, 22:20

... Inc., Cleveland, Ohio, USA) to be used in this study is isolated from a single bone marrow aspirate and cultured with heat inactivated fetal bovine serum (FBS) and growth factors EGF and PDGF Cells ... cells, including MAPCs and MSCs, have been shown to beneficially modulate the anti-donor immune response in organ transplantation and to promote tissue regeneration in vitro and in vivo [26-29,53] ... FCP, and HJS BF developed essential study documents EKG, PS, and PP supported the design of the study with their knowledge and experience MHD is the principal investigator of the study and the...

Ngày tải lên: 20/06/2014, 04:20

... both pulmonary and systemic inflammation, with induction of cytokines and chemokines and generation of oxidative species These injurious molecules create and exacerbate inflammation and oxidative ... angiographic results in phases and (the pilot phase and first randomized phase) For multivariate analysis, the treatment groups were categorized as follows: control, 0; CPC, 1; and BMC, The multivariate ... Abciximab and Device Investigation to Lower Late Angioplasty Complications] trial) and 4.1% (in the ADMIRAL [Abciximab before Direct Angioplasty and Stenting in Myocardial Infarction Regarding Acute and...

Ngày tải lên: 27/06/2014, 00:20

... BCNU and cisplatin, of which the former is primarily used for treating brain cancers and Hodgkin’s lymphoma and the latter is used to treat a wide range of cancers (including breast, lung and ... cells, and NSCs (Figure 3) The preferential vulnerability of both rat and human primary CNS progenitor cells to BCNU and cisplatin was apparent also at very low exposure levels Even the BCNUand ... respectively, on days 1, 3, and 5) show marked and prolonged increases in cell death in the lateral subventricular zone (SVZ), the corpus callosum (CC) and the dentate gyrus (DG) at 1, 10, and 42 days following...

Ngày tải lên: 06/08/2014, 18:21

... (CD44) and intercellular adhesion molecule-1 (CD54), growth factor and cytokine receptors, integrins and additional markers such as tetraspan (CD9) and Thy-1 (CD90) In contrast, CD34 and the ... dioxide, and the medium was changed every third day After weeks the samples were used for histological and immunohistological studies, and for RT-PCR gene expression analysis Histology and immunohistochemistry ... (45 s at 72°C) and denaturation (60 s at 94°C); and terminating with 15 at 72°C PCR products were separated on a 1.5% agarose gel and stained with ethidium bromide, visualized and digitalized...

Ngày tải lên: 09/08/2014, 01:23

... the relevance of these findings in terms of our understanding of RA? Increased angiogenesis is thought to be crucial in supplying nutrients and oxygen to the synovial tissue in RA, which by virtue ... result in a poorer response to ischaemia, and thereby cardiovascular events such as stroke or myocardial infarction This is supported by the findings of Smolen and colleagues’ study, which quantified ... angiogenic EPC were reduced when expanded from patients with active RA, compared with inactive or healthy controls [7] What conclusions the findings of EPC in RA synovium and reduced circulating EPC...

Ngày tải lên: 09/08/2014, 07:20

... Groningen, The Netherlands) and CD133 (Miltenyi Biotec, Utrecht, The Netherlands) Cells were washed and resuspended in PBS Measurements were performed on the FACSCalibur and CellQuest software ... of patients and participated in design and drafting of the manuscript MH participated in the design and coordination of the study and helped to draft the manuscript All authors read and approved ... for relations between complement levels and levels of antibodies against double-stranded (ds) DNA and the experimental outcomes (for example, number of CPCs and CFU formation) A probability value...

Ngày tải lên: 09/08/2014, 10:20

... T-MPCs, and adherent cells were obtained similarly Both T-MPCs and BM-MPCs were culture-expanded in basal medium at 37°C and 5% CO2 using T-150 Triple Flask (Nunc, Roskilde, Denmark), and medium ... including T-MPCs and BM-MPCs of the same passage maintained and cultured in expansion medium, and BM-MPCs cultured in adipogenic medium Morphological changes in BM-MPCs and T-MPCs and the formation ... negative for CD14, CD34, and CD45 and positive for CD29, CD44, and CD105 Bars = 20 μm (b) Flow cytometric analysis of T-MPCs and BM-MPCs CD14, CD34, CD45, CD105, CD73, and CD90 were detected by...

Ngày tải lên: 09/08/2014, 10:23

... cells were expanded by culturing in vitro and challenged with the T cell tropic HIV-1 NL4-3 On different days post-infection, samples were collected and assayed for p24 antigen Tar & and Tar-CCR5Rz ... overall experimental design and implementation of the project Acknowledgements Work reported here was supported by NIH grants AI50492 and AI057066 to R.A and AI 42552 and AI2932 to JR This work ... levels were 75% and 30% (Fig 6, panels A and B) Reconstitution levels are known to vary considerably between mouse to mouse based on the variable sizes of the grafts injected and possibly due...

Ngày tải lên: 10/08/2014, 05:20

... (ngn2) and GAD67 from Millipore (Temecula, CA) and antibodies against Mash1/Achaete-scute homolog 1(Mash1), PSD95, NeuroD1, vGluT1, PCNA and BrdU were obtained from Abcam (Cambrigeshire, England) ... treated with ethanol and Western blot and immunocytochemistry were performed to determine the expression of Pax6 and downstream transcription factors (A) as well as glutamatergic and GABAergic neuronal ... other hand, the level of GAD67 was reduced in the cortex and hippocampus of prenatally ethanol-treated group The level of Tuj-1 and bactin determined by Western blot (Figure 4B) as well as NeuN and...

Ngày tải lên: 10/08/2014, 05:21

... ESC-like cells, and generation of induced pluripotent stem cells (iPS) [11-13] We and others have determined that Sall4 exists as two isoforms (Sall4A and Sall4B), and they have unique and overlapping ... measured at 2, 20, and 50 weeks after transplantation of indicated lentivirus-infected BM cells Error bars represent standard error of the mean Figure The lineage (Gr-1, B220 and CD4/CD8) and primitive ... transplanted with Sall4 expanded cells Strain Number of mouse C57B/6 Sall4A expanded cells, × 106 17 WT-like B6.SJL Sall4A expanded cells, × 106 15 WT-like B6.SJL Sall4A expanded cells, × 106 11 WT-like...

Ngày tải lên: 10/08/2014, 21:23

... difference in fluorescent emission peaks (616 nm and 613 nm) when 458 nm and 514 nm LASER wavelengths are used for cluster and in Figs 4(a-b) and 6(a-b) The most probable reason is the merging ... the 514-nm laser power The energy band structure of the CdSe QD is schematically shown in Fig 7, where CB denotes the conduction band edge and VB the valence band edge Referring to the vacuum level ... -4.95 eV (electron affinity of CdSe), the band gap Eg (energy difference between CB and VB) is 1.74 eV, and the quantum confinement energy for the valence band hole is 1.5 eV [12] For our CdSe QDs...

Ngày tải lên: 11/08/2014, 00:22

... to lung regeneration [17,18] and we not know yet, how the specific microenvironment of the diseased lungs alters fate and function of endogenous or therapeutically administered stem and progenitor ... myofibroblasts and macrophages, we expanded prominin-1+ cells from lung tissue in the culture expansion medium (CEM) [12] Expanded prominin-1+ cells were negative for fibronectin (Figure 1A) and other ... other hand, prominin-1+ cells lost prominin-1 expression and became F4/80+ macrophages (Figure 7C) However, prominin-1+ cells expanded from inflamed lungs failed to up-regulate Sftpc expression and...

Ngày tải lên: 12/08/2014, 14:20

... end-inspiration, the heart-lung block removed and 16 gauge cuffs (Abbocath-T, Abbott, Sligo, Ireland) were placed around the pulmonary artery and vein The vessels were inverted and tied onto the cuff with an ... shows the forward and side scatter characteristics of the blood sample In panel B the latex beads which are used as internal quantification standard (gate Beads) and the CD45 and CD42a negative ... increased blood flow and capillary density [68] and a significantly lower number of lost limbs due to the transplantation of human EPC in athymic nude mice [69] A pilot study and randomized controlled...

Ngày tải lên: 12/08/2014, 15:20

... noncytotoxic and have no impact on cell function, such as cell proliferation and differentiation [23] Due to the brightness and high emission signals of QDs, resistance to photobleaching and retention ... [14-16], brain [17] and lungs [18-21] The majority of these studies have used models of toxic, traumatic or ischemic tissue injury, and showed engraftment of both type II [20,21] and type I [19] ... sequestration in the BM and lung, and their migration into airspaces in a mouse model of pneumonia Methods Animals Female C57BL/6J mice (10–12 weeks old) were used as donors and recipients Mice were...

Ngày tải lên: 12/08/2014, 15:21

... and colleagues [21] (see Materials and methods) Interestingly, the distribution of the CD166+ cells in OA and normal samples was not uniform but was clearly zonal (Figures and 7) In both OA and ... Roth, Andreas Wagner, and Rando Winter (Clinic of Orthopedics, University Hospital Jena, Waldkrankenhaus ‘Rudolf Elle’, Eisenberg) for providing patient material and to Cordula Müller and Jana ... contributions DP and SA contributed to study conception and design, acquisition of data, analysis and interpretation of data, and drafting or critical revision of the manuscript or both CK and RWK contributed...

Ngày tải lên: 12/08/2014, 15:23