2stem cell expansion and differentiation using biomaterials

... of medium The cells from the unaffected and affected sides expanded with, on average, 0.21 and 0.22 cell doublings per day, respectively After one passage (4.3 cell doublings) and weeks of culture, ... stabilizes pH and osmolarity and supplements the cells with trace compounds and energy sources (pyruvate and glucose) The added serum contains high levels of growth factors released by cells and platelets ... flattened cells on the surface and round cells in the centre (Fig 3) Spindle-shaped cells were found in the central part of the pellet in some donors, and the frequency of spindleshaped cells was...

Ngày tải lên: 09/08/2014, 06:22

... below 2.1.2.1.3 Cell lysis CHO cells were collected and re-suspended in suitable amount of SPHK buffer (described below) Cells were lysed by several cycles of freeze-thawing and total cell lysates ... U937 cells viability 5,000 and 10,000 cells were treated with 25μM of DMS or different compounds for 24 hours or 48 hours Cells were then stained with Trypan blue and counted under microscope Cells ... concentrations: 10μM, 25μM, 50μM, 75μM, and 100μM The lipids were separated using the TLC method as for the SPHK assay, and the results were visualized and quantified using TyphoonTM phosphor-imager...

Ngày tải lên: 11/09/2015, 16:06

... activity in the stem cells, the balance between proliferation and differentiation could be lost, the cell growth was arrested, and the cells would then enter into certain differentiation pathway(s) ... 5,000cells/cm2 in all of the experiments using human BM- and AD- MSCs 3.1.1.4 Stem Cells Osteogenic and Adipogenic Induction Culture Condition Normal growth Adipogenic differentiation Osteogenic differentiation ... inhibit at all cell growth compared with untreated cells (DMSO=0) 3.2.3 Function of CP6 and DMS in Human BM- and AD- MSCs Differentiation Human BM- and AD- MSCs are multipotent cells that are...

Ngày tải lên: 11/09/2015, 16:06

... the stem cells remained as stem cells Starting cells for human BM-MSCs were passage 6, and for human AD-MSCs (cells from two different donors) were passage Cells were equally grouped and cultured ... 4.1 Cell surface markers expression in human BM- and AD- MSCs Table B and C represent data from two donors cells of AD-MSCs Mouse IgG1 and IgG2a were used as the isotype controls and five thousand ... 38% more than cells cultured in 1% FBS alone; and cells cultured in 1μM S1P+1% FBS showed 52% more cell growth than cells cultured in 1% FBS alone 100nM S1P did not show to promote cells growth...

Ngày tải lên: 11/09/2015, 16:06

... study other stem cells and other differentiations pathways, to further validate and confirm the potential usage of SPHK inhibitors in stem cell differentiation, and S1P roles on stem cell proliferation ... stem cells (i.e MSCs), the knowledge accumulated on cell expansion and differentiation might also be applicable in other types of stem cells Also, it should be noticed that both human BMand AD- ... osteogenic differentiation and adipogenic differentiation in human MSCs differently In human BM- and AD- MSCs osteogenic differentiation, 0.5μM CP6 and DMS showed to promote the osteogenic differentiation...

Ngày tải lên: 11/09/2015, 16:06

... intracellular sphingosine-1-phosphate and promotes cell growth and survival." J Cell Biol 147(3): 545-58 Olivera, A and S Spiegel (1993) "Sphingosine-1-phosphate as second messenger in cell proliferation ... sphingosine-1-phosphate and platelet-derived growth factor in the maintenance of human embryonic stem cells." Stem Cells 23(10): 1541-8 Peng, H and J Huard (2003) "Stem cells in the treatment of muscle and connective ... "Whole genome analysis of human neural stem cells derived from embryonic stem cells and stem and progenitor cells isolated from fetal tissue." Stem Cells 25(5): 1298-306 Shmelkov, S V., S Meeus,...

Ngày tải lên: 11/09/2015, 16:06

... one hand, it could act as an intracellular mediator and trigger numerous cellular events, including intracellular calcium release, and promote cell survival and cell growth On the other hand, ... stem cells and the strategies used for stem cell expansion and differentiation, are discussed below 1.4 HUMAN STEM CELLS 1.4.1 Brief Introduction of Human Stem Cells Diverse stem cells and their ... messenger and as an extracellular ligand for specific cell- surface receptors to regulate cell survival, in either case S1P has been shown to promote cell proliferation, morphological changes and migration...

Ngày tải lên: 11/09/2015, 16:06

... both cell growth and cell proliferation through multiple pathways and that there is neither a single critical effector gene nor a small group of such genes In cell culture, Myc can affect cell ... targets of Myc in cell proliferation and their proposed functions Control of Cell Proliferation and Growth by Myc Proteins 333 2003) There is clear evidence that this effect is direct and not mediated ... required for cell proliferation and cell growth or whether its essential role in proliferation is restricted to specific cell types Control of Cell Proliferation and Growth by Myc Proteins 331...

Ngày tải lên: 25/10/2013, 21:20

... b-catenin, total b-catenin and collagen-I, and cell proliferation were determined As shown in Figs 7A,B and 8A,B, the incubation of lung fibroblasts with a-defensin-1 and a-defensin-2 induced increases ... kinase and PI3K ⁄ Akt and increase phosphorylation of GSK3b on Thr390 and Ser9 SB203580 and LY294002 prevented a-defensin-induced increases in the protein contents of Thr390-phosphorylated and Ser9-phosphorylated ... dephosphorylated b-catenin, and collagen-I (Figs 7A–C and 8A–C) Moreover, a-defensin-induced increases in cell proliferation were prevented by SB203580 and LY294002 (Figs 7D and 8D) FEBS Journal 276...

Ngày tải lên: 18/02/2014, 06:20

... proliferation rate and cell death In various cell models, such as kidney epithelial cells [49], hepatocytes [50] or carcinoma cells [51], cytochalasin has been shown to induce cell detachment from ... promote cell proliferation [58] Interestingly, recent work has shown that the cytoskeleton orients the cell metabolic and signal transduction machinery, and that mechanical distortion of cells and ... extracellular matrix on differentiation and apoptosis Curr Biol 1997, 7(1):43-51 Huet C, Pisselet C, Mandon-Pepin B, Monget P, Monniaux D: Extracellular matrix regulates ovine granulosa cell...

Ngày tải lên: 05/03/2014, 17:20

... 0.01 compared with control cells DHEA inhibited cell proliferation and decreased cell viability Three cell lines were evaluated: non-HPV-infected cells (C33A) and cells infected with human papilloma ... estrogen and androgen, because use of antagonists to androgen and estrogen receptors (flutamide and ICI 182,780, respectively), and letrozol, an inhibitor of the aromatase responsible for converting androgen ... USA) Cell culture CASKI, HeLa and C33A cells were purchased from the American Type Culture Collection (Manassas, VA, USA) CASKI and HeLa cell lines were maintained in RPMI-1640 medium and C33A cells...

Ngày tải lên: 16/03/2014, 00:20

... facilitate cell survival and to prevent apoptotic cell death In fact, dominant negative alleles of Akt reduce the ability of growth factors, extracellular matrix and other stimuli to support cell survival ... Akt-dependent cellular proliferation and cellular survival in human cancer cells by numerous GPCRs has opened up the possibility of controlling cellular events through the use of ligands for a variety ... cell migration Trends Cell Biol 16, 461– 466 Downward J (2004) PI 3-kinase, Akt and cell survival Semin Cell Dev Biol 15, 177–182 Hanada M, Feng J & Hemmings BA (2004) Structure, regulation and...

Ngày tải lên: 16/03/2014, 05:20

... the number of bands and the labeling intensity were increased in growing cells The most prominent increases were in bands of 140, 133, 116, 95, 82, 73 and 56 kDa (Fig 4) Other bands also showed ... electrophoresis in 1.7% agarose gels and visualized with SYBR Green I Data measurement and statistical analysis Radiotracer, cell number and enzyme assay data were quantified and plotted as means ± SEM ... record cell morphology Representative micrographs are shown Specific treatments are: untreated control (A), 50 lM MIBA (B) and MIBG (C), 100 lM MIBA (D) and MIBG (E), 200 lM MIBA (F) and MIBG (G), and...

Ngày tải lên: 17/03/2014, 09:20

... Table 1, and DP8 was assessed by cell stain (not shown) DP8 and DP9 have been localized to Golgi and endoplasmic reticulum [7,8] Concordantly, in the 293T cells transfected with DP8–GFP and DP9–GFP, ... and DP9 overexpression to impair monolayer wound healing on ECM DP8 and DP9 impaired cell migration Cell migration was also assessed in transwells In vitro cell migration assays showed that cells ... in vitro wound healing, cell migration and apoptosis, and for DP8 in wound healing, cell migration and apoptosis enhancement (Table 4) DP9 overexpression impaired 2452 cell behavior with regard...

Ngày tải lên: 23/03/2014, 11:20

... the cytoplasmic domain of integrins Maintenance of cell matrix contact is an important cell survival factor and the loss of cell matrix and cell cell contact is a characteristic feature of apoptosis ... per lane) were subjected to SDS ⁄ PAGE and western blotting using specific antibodies against p130Cas (A and B), PKCd (C and D, upper panel) and actin (C and D, lower panel) as described Molecular ... of the experiment, cells were lysed and total proteins (20 lg per lane) were subjected to SDS ⁄ PAGE and western blotting using specific antibodies against FAK (A), p130Cas (B) and actin (C) as...

Ngày tải lên: 23/03/2014, 11:20

... control cells, B, D, and F paclitaxel (10 nM for 24 h) treated cells, and H dbcAMP (0.5 mM for 72 h) treated cells In A, B, G and H, cells were stained with PP17, in C and D with anti-bax, in E and ... 6B) Cells were treated with dbcAMP or PMA to obtain data on PP17 gene involvement in cell differentiation pathways, and both notably induced differentiation and PP17 protein synthesis (Fig 5H and ... induced cells by grey bars (B and C) Cell cycle-dependent PP17 fluorescence of control and paclitaxel-induced cells Time-dependent shift in cell cycle was caused by paclitaxel, stopping cells in...

Ngày tải lên: 23/03/2014, 21:20

... semi- structural and finally to structural adhesives Everaerts and Clemens provide a thorough description of chemistry and applications of pressure sensitive adhesives and Kinning and Schneider ... between practical adhesion energy and the work of adhesion is the generalized fracture mechanics theory of Gent and coworkers [23-251 and contributed to by Andrews and Kinloch [26-291 This defines ... adhesives and is described by Parbhoo et al in Chapter 14 Progressing into more semi-structural and structural adhesives, the chapters by Paul and by Frisch describe the chemistry and properties...

Ngày tải lên: 26/04/2014, 15:36

... http://www.translational-medicine.com/content/9/1/97 Cell lines and culture conditions The human colorectal cancer cell lines LoVo, SW480, HT29, HCT116 and LS174T were maintained in RPMI 1640 (Gibco, Grand Island, NY, USA) supplemented ... cytometry assay Cells were harvested at an exponential growth phase, and single -cell suspensions containing × 106 cells were fixed with 70% alcohol The cell cycle was monitored using propidium ... endogenous p53 and p21 expression and decreased endogenous C-myc expression (Figure 4C) Thus, it can be inferred that the inhibition of cell proliferation and cell cycle arrest in CK2a knockdown cells...

Ngày tải lên: 18/06/2014, 19:20

... material and immune cell development involves in human carcinogenesis In this study, we studied about the toxic effects of borax on immune cell proliferation (lymphocyte proliferation) and genotoxicity ... chromosome-banding patterns In human chromosomes, SCEs occur preferentially in Q-negative bands or at the junction of Q-positive and Q-negative regions [10] The toxic effects of borax to immune cell ... Medicine and Toxicology 2009, 4:27 http://www.occup-med.com/content/4/1/27 (PBS) were added to each well and incubated for hours at 37°C The medium was removed and formazan was dissolved in DMSO and...

Ngày tải lên: 20/06/2014, 00:20