2ecm and the stem cell niche

... for the growth of the anchorage-dependent cells and the culture medium stabilizes pH and osmolarity and supplements the cells with trace compounds and energy sources (pyruvate and glucose) The ... flattened cells on the surface and round cells in the centre (Fig 3) Spindle-shaped cells were found in the central part of the pellet in some donors, and the frequency of spindleshaped cells was ... seem to affect the proliferation ability and disappeared after the first change of medium The cells from the unaffected and affected sides expanded with, on average, 0.21 and 0.22 cell doublings...

Ngày tải lên: 09/08/2014, 06:22

... 2cm from the top of the plate, then removed from the chamber and air dried in a fume hood Radioactive signal on the TLC plate was captured by exposing the plate to a TyphoonTM scanner and the intensity ... fact, in the concentrations tested (10μM, 25μM, 50μM, 75μM, and 100μM), none of the six compounds inhibited DAGK and human PKC alpha (Figure 2.8 and Figure 2.10) On the other hand, and in agreement ... of the assay (~5-25μg/mL), the more protein present, the more the Coomassie Blue binds changing the absorbance of the sample After getting cell lysates from the CHO cells, over-expressing SPHK1...

Ngày tải lên: 11/09/2015, 16:06

... hypothesized that when suppressing SPHK activity in the stem cells, the balance between proliferation and differentiation could be lost, the cell growth was arrested, and the cells would then ... 3.1.1.3 Stem Cells Seeding Density Unless otherwise stated, stem cell seeding density used was 5,000cells/cm2 in all of the experiments using human BM- and AD- MSCs 3.1.1.4 Stem Cells Osteogenic and ... penetrate into the cells and inhibit the endogenous SPHK1 and the good yield obtained from the chemical synthesis procedure Therefore, in this chapter, CP6 was selected to study the function of...

Ngày tải lên: 11/09/2015, 16:06

... that the stem cells remained as stem cells Starting cells for human BM-MSCs were passage 6, and for human AD-MSCs (cells from two different donors) were passage Cells were equally grouped and ... passage they were considered to maintain their multipotency Cells cultured in S1P-supplemented media were then measured for their stemness maintenance by checking their morphology change, the cell ... promote stem cell proliferation (Figure 4.5, Figure 4.6, and Figure 4.7) The cells were washed in PBS and starved in serum-free DMEM for half an hour before 1μM S1P was added into the cells and incubated...

Ngày tải lên: 11/09/2015, 16:06

... study other stem cells and other differentiations pathways, to further validate and confirm the potential usage of SPHK inhibitors in stem cell differentiation, and S1P roles on stem cell proliferation ... tissue-specific stem cells (i.e MSCs), the knowledge accumulated on cell expansion and differentiation might also be applicable in other types of stem cells Also, it should be noticed that both human BMand ... primary cells, and thus they may possess certain variations between different donor cells Therefore, more work need to be done to further confirm our conclusions Finally, the study could be expanded...

Ngày tải lên: 11/09/2015, 16:06

... directing the differentiation of stem cells into the osteogenic lineage in vitro.” J Bone Miner Res 19:1379–1394 Hentze, H, Graichen R, et al (2007) Cell therapy and the safety of embryonic stem cellderived ... platelet-derived growth factor in the maintenance of human embryonic stem cells." Stem Cells 23(10): 1541-8 Peng, H and J Huard (2003) "Stem cells in the treatment of muscle and connective tissue diseases." ... induction and PGE2 production in response to TNFalpha." Faseb J 17(11): 1411-21 Phinney, D G and I Isakova (2005) "Plasticity and therapeutic potential of mesenchymal stem cells in the nervous system."...

Ngày tải lên: 11/09/2015, 16:06

... human stem cells and the strategies used for stem cell expansion and differentiation, are discussed below 1.4 HUMAN STEM CELLS 1.4.1 Brief Introduction of Human Stem Cells Diverse stem cells and their ... On the one hand, it could act as an intracellular mediator and trigger numerous cellular events, including intracellular calcium release, and promote cell survival and cell growth On the other ... the roles of SPHK and S1P in stem cell proliferation, stemness maintenance and differentiation In particular, we are interested in the role of S1P in human adult stem cell proliferation and stemness...

Ngày tải lên: 11/09/2015, 16:06

... laboratory is supported by the Deutsche Forschungsgemeinschaft, the European Community through the Framework program, the Thyssen- and the Sander-Stiftung, AICR and the Deutsche Krebshilfe References ... both cell growth and cell proliferation through multiple pathways and that there is neither a single critical effector gene nor a small group of such genes In cell culture, Myc can affect cell ... (lineage positive) cells arrest (Wilson et al 2004) While these data show that c-myc is not required for proliferation of hematopoietic stem cells, such cells also express n-myc and therefore it is...

Ngày tải lên: 25/10/2013, 21:20

... In the present study, we also found that a-defensin-1 and a-defensin-2 stimulated the proliferation and collagen synthesis of two other cell lines of lung fibroblasts (HFL-1 and LL86) However, the ... collagen release from the cells, the collagen content in the culture medium of lung fibroblasts (HFL-1) treated without or with a-defensin-1 and a-defensin-2 (2.5 lm) in the presence and absence of quercetin ... b-catenin and collagen-I, and cell proliferation were determined As shown in Figs 7A,B and 8A,B, the incubation of lung fibroblasts with a-defensin-1 and a-defensin-2 induced increases in the protein...

Ngày tải lên: 18/02/2014, 06:20

... G1 and entry into the S phase of the cycle [50], and that signals elicited by the cytoskeleton act independently of ERK1/2 signals to drive the cell cycle machinery through the G1/S boundary and, ... promote cell proliferation [58] Interestingly, recent work has shown that the cytoskeleton orients the cell metabolic and signal transduction machinery, and that mechanical distortion of cells and the ... significant correlations between the percentage of round cells and the proliferation rate of GC from small follicles (r = 0.87, p < 0.001) on the one hand (Fig 8a), and the pyknotic rate of GC from...

Ngày tải lên: 05/03/2014, 17:20

... tumor cells There was a non-statistically significant difference in the response of the cell lines to treatment with DHEA C33A and CASKI cells were more responsive to DHEA, and HeLa cells were the ... to the malignant state of the cells HeLa cells are an advanced-stage cervical cell carcinoma [27], in comparison with the other cell lines used for which no stage is specified; therefore, HeLa cells ... metabolites The antiproliferative effect of DHEA has been associated with an arrest of the cell cycle and cell death in BV-2 cells, a murine microglial cell line, in hepatoma cell lines and in HepG2 cells...

Ngày tải lên: 16/03/2014, 00:20

... suggests that the GPCR activation of Akt pathways can be either proliferative or antiproliferative, depending on the nature of the stimulus and the cell type observed Competing effects on cell cycle ... reduce the ability of growth factors, extracellular matrix and other stimuli to support cell survival Conversely, the overexpression of Akt can rescue cells from apoptosis [9] This is achieved by the ... which are then exported from the nucleus and sequestered in the cytoplasm by their interaction with 14-3-3 proteins [12] Akt-dependent cell survival may also be achieved by the activation of the nuclear...

Ngày tải lên: 16/03/2014, 05:20

... for Cells were scraped from the upper side of the membrane and the membrane was subsequently placed into Giemsa stain for 60 The membrane was mounted on a slide and the cells present on the underside ... however, both the number of bands and the labeling intensity were increased in growing cells The most prominent increases were in bands of 140, 133, 116, 95, 82, 73 and 56 kDa (Fig 4) Other bands also ... mgÆmL)1) and 20 lL cell extract (microsomal or cytosolic fraction) and incubating this mixture for 30 at 30 °C [27] The reaction was stopped by the addition of mL cold 20% trichloroacetic acid and the...

Ngày tải lên: 17/03/2014, 09:20

... Despite their close sequence relatedness, DP8 and DP9 exert these differences in their cellular effects Therefore, these two proteins are likely to have different functions and ligands These data ... cells have further established these roles in cell ECM interactions [12] Cell adhesion is crucial in monolayer wound healing and cell migration Therefore, the adhesion defect of cells overexpressing ... threshold and lowpass filter was used to count migrated cells by measuring the total area covered by cells (bright field) and the area covered by fluorescence-positive cells in the wound and nonwound...

Ngày tải lên: 23/03/2014, 11:20

... cell types undergoing apoptosis further suggests the important role played by the association of focal adhesion proteins, the cytoskeleton and the extracellular matrix in the maintenance of cell ... linking actin filaments to the cytoplasmic domain of integrins Maintenance of cell matrix contact is an important cell survival factor and the loss of cell matrix and cell cell contact is a characteristic ... contribute to establish the role of the mevalonate pathway in the physiological growth and development of cells from the central nervous system Results Effect of lovastatin on the cleavage of focal...

Ngày tải lên: 23/03/2014, 11:20

... Each protein band yielded a good quality peptide map, and most of the input masses were matched to the candidate protein sequences The majority of the tryptic peptides matched with the theoretical ... cytomorphological alterations in the nucleus and cytoplasm, and by the elevated bax/bcl2 oncoprotein ratio, widely used for squamous epithelial cells and tissues [25] The effect and time-course of different ... dewaxed and rehydrated Both tissue sections and the cell culture samples described above were incubated with anti-PP17 antibody, and with monoclonal anti-bax and anti-bcl2 antibodies for the parallel...

Ngày tải lên: 23/03/2014, 21:20

... where amolis the molar area of the adsorbate gas, and the constant accommodates the choice of standard states If the only interaction between the adsorbate and the adsorbent is of the Lifshitz-van ... both of the chemistry of the components and of the morphology of the adherend surface on the observed contact angle Finally, comment must be made on the dynamics of the wetting process and the factors ... polyethylene substrate On the other hand, if the epoxy was first cured, and then molten polyethylene were applied to it as the adhesive, the adhesion was excellent Since the same interface was created...

Ngày tải lên: 26/04/2014, 15:36

... experiments JZ and HL performed the statistical analysis and drafted the manuscript DW and LL helped in drafting the manuscript and contributed specific information and critical analysis throughout the ... inhibited cell proliferation and promoted cell senescence After CK2a knockdown, the percentage of G0/G1 phase cells significantly increased (t = -9.577, P < 0.01), and the percent of S phase cells ... according to the percentages of the positive staining areas relative to the entire carcinoma-involved area or, for the normal samples, the entire section The sum of the intensity and extent scores...

Ngày tải lên: 18/06/2014, 19:20

... related to the number of living cells When the amount of purple formazan produced by cells treated with an agent is compared with the amount of formazan produced by untreated control cells, the effectiveness ... results and the cytotoxicity index (CI) was calculated for each borax concentration The borax concentration of 0.6 mg/ml had the most effectiveness to the lymphocyte proliferation and had the highest ... this study (Figure 4) The 50% inhibitory concentration (IC50) was 0.9 mg/ml (unpublished data) The results indicated the correlation between the immune cell proliferation and the borax cytotoxicity...

Ngày tải lên: 20/06/2014, 00:20