2 materials and methods
THE SPATIOTEMPORAL STUDY OF ZEBRAFISH INTESTINAL EPITHELIUM RENEWAL
Ngày tải lên :
10/09/2015, 09:25
... mammalians 20 Intestinal stem cells (ISCs) studies 22 Lineage tracing 22 In vitro culture 24 Label retention (BrdU and EdU) 24 Mosaic generation 25 Chapter ... 2 Materials and Methods 32 Results and discussion 35 2 The spatial orientation of zebrafish intestinal epithelium renewal –by negative marking of epithelial cells 44 2 ... 58 Materials and Methods 61 In vivo labelling of proliferating intestinal epithelium cells 61 2 Tissue sampling 61 3 Imaging and statistical analyzes 62 3 Results and...
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Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc
Ngày tải lên :
12/08/2014, 11:23
... no competing interests 21 Received: 19 November 20 09 Revisions requested: 28 January 20 10 Revised: 29 January 20 10 Accepted: 22 February 20 10 Published: 22 February 20 10 22 References Pittenger ... cells have in vivo 23 24 25 26 27 28 29 30 31 32 33 34 35 immunosuppressive properties applicable for the control of the graftversus-host disease Stem Cells 20 06, 24 :25 82- 2591 Sudres M, Norol ... adoptive transfer of CD25+ regulatory T cells Arthritis Rheum 20 05, 52: 221 2 -22 21 doi:10.1186/ar2939 Cite this article as: Schurgers et al.: Discrepancy between the in vitro and in vivo effects of...
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Retrovirology Research BioMed Central Open Access In vivo expression of the HBZ gene of HTLV-1 doc
Ngày tải lên :
12/08/2014, 23:20
... 0.5 12 0.117 0.071 0 .28 5 0.001 0.431 0.639 0. 328 0.437 0.185 0.0 42 0. 023 0.00 52 0.194 0.801 0 .24 1 0 .22 8 0 .23 4 -0.0 029 0.401 0.544 0.333 0. 322 0 .25 2 0. 121 0.1 02 -0.046 0.091 0.4 42 0.497 0.690 0 .25 7 ... HTLV-I Jpn J Cancer Res 19 92, 83 :23 6 -23 9 Page of 11 (page number not for citation purposes) Retrovirology 20 09, 6:19 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 Sugimoto M, Nakashima ... 2. 4 25 .8 0.0 62 26.35 0.011 1 .24 1.71 87.4 8.1 19.3 7.1 16 .2 12. 6 115.5 1.64 0.063 3.56 0.48 0.19 0 .22 0.0076 1.37 0.0015 0.077 0.135 0.756 0.74 (0. 023 –33.50) 0.15 (0.0013–6. 42) 31.43 (5.93 22 5.64)...
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Báo cáo y học: " The connection domain in reverse transcriptase facilitates the in vivo annealing of tRNALys3 to HIV-1 genomic RNA" potx
Ngày tải lên :
13/08/2014, 13:20
... Virol 1990, 64:5076-50 92 Cen S, Khorchid A, Gabor J, Rong L, Wainberg MA, Kleiman L: The role of Pr55gag and NCp7 in tRNALys3 genomic placement 22 23 24 25 26 27 28 29 30 31 32 and the initiation ... 68 :20 65 -20 72 Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochemistry 1976, 72: 248 -25 4 ... BH 10 P- C - Gag 100 75 50 25 Figure The incorporation of GagPol and tRNALys3into wild-type and mutant HIV-1 The incorporation of GagPol and tRNALys3into wild-type and mutant HIV-1 A Schematic...
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Báo cáo sinh học: "A dual function fusion protein of Herpes simplex virus type 1 thymidine kinase and firefly luciferase for noninvasive in vivo imaging of gene therapy in malignant glioma" ppsx
Ngày tải lên :
14/08/2014, 19:22
... fluorescent tag FEBS Lett 20 02, 527 :153-158 http://www.gvt-journal.com/content /2/ 1/7 23 24 25 26 27 28 29 30 31 32 33 34 35 36 Ray P, Wu AM, Gambhir SS: Optical bioluminescence and positron emission ... 11 of 13 (page number not for citation purposes) Genetic Vaccines and Therapy 20 04, 2: 7 10 11 12 13 14 15 16 17 18 19 20 21 22 Shand N, Weber F, Mariani L, Bernstein M, Gianella-Borradori A, Long ... Lancet 20 01, 358: 727 - 729 Page 12 of 13 (page number not for citation purposes) Genetic Vaccines and Therapy 20 04, 2: 7 http://www.gvt-journal.com/content /2/ 1/7 Publish with Bio Med Central and every...
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Stability and in vivo evaluation of pullulan acetate as a drug nanocarrier
Ngày tải lên :
23/04/2013, 21:38
... mice at 20 0 mg/kg dose ( ± s, g) Group Sex n Day Day Day 15 18.46 ± 0.81 23 . 12 ± 1.51 24 .90 ± 1.04 Control 18.64 ± 1.17 25 .68 ± 2. 81 30. 02 ± 2. 25 18.14 ± 0.68 22 .85 ± 1. 12 18.68 ± 0.51 26 .30 ± 0.80 ... (nm) PDI ζ potential (mV) Day 26 0.4 ± 8.03 0.180 ± 0. 025 −3.353 ± 1 .29 6 month 24 2.4 ± 27 .15 0.1 62 ± 0.073 2. 058 ± 1.987 month 22 9.5 ± 17.78 0.136 ± 0.085 −1.580 ± 2. 153 Liver Spleen Table 3. ... Altogether, PA/EPI had the longer t1 /2 and MRT, lower Vd and CL than the free drug in rats At the same time, the AUC of EPI and PA/EPI from 0 24 h were 48, 128 .26 9 and 91,006.508 µg h/L, respectively...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
Ngày tải lên :
22/02/2014, 07:20
... described previously [29 ] with the E coli strain BW25113 This strain behaves as E coli MC4100 or TG2, with regard to its UP 12 expression pattern (data not shown), and as Ó FEBS 20 02 UP 12 is an E coli ... mM EDTA and 20 mM dithiothreitol] and analyzed by SDS/PAGE and Western blotting Subcloning and deletion of the chromosomal ybdQ gene (encoding UP 12) The chromosomal ybdQ gene encoding UP 12 (locus ... background indicates identical and similar residues, respectively Swiss-Prot accession numbers are as follows: UspA, P2 824 2 [ 12] , YiiT, P 321 63; UP03, P37903; UP 12, P39177; YecG, P46888 [15] 3036...
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Báo cáo khoa học: In vivo production of catalase containing haem analogues pot
Ngày tải lên :
06/03/2014, 11:20
... haemochromogen ¨ 26 70 FEBS Journal 27 7 (20 10) 26 63 26 72 ª 20 10 The Authors Journal compilation ª 20 10 FEBS M Brugna et al analysis [ 32] , and rocket immunoelectrophoresis Immunoblot and rocket immunoelectrophoresis ... Watanebe Y (20 02) Construction of haem enzymes: four approaches Curr Opin Chem Biol 6, 20 8 21 6 FEBS Journal 27 7 (20 10) 26 63 26 72 ª 20 10 The Authors Journal compilation ª 20 10 FEBS 26 71 Cofactor-substituted ... Journal 27 7 (20 10) 26 63 26 72 ª 20 10 The Authors Journal compilation ª 20 10 FEBS 26 67 Cofactor-substituted catalase M Brugna et al maxima (396, 499, 531 and 619 nm as compared with 406, 504, 541 and...
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Báo cáo khoa học: A steady-state modeling approach to validate an in vivo mechanism of the GAL regulatory network in Saccharomyces cerevisiae ppt
Ngày tải lên :
07/03/2014, 16:20
... G8 02 þ ½D2G 42 G 42 þ ½D2G 42 G8 02 G 42 þ ½D2G 42 G8 02 G 42 G8 02 þ ½D2G 42 G8 02 G3* þ ½D2G 42 G8 02 G3*G3* þ ½D2G 42 G8 02 G3*G 42 þ ½D2G 42 G8 02 G3*G3*G 42 þ ½D2G 42 G8 02 G3*G 42 G8 02 þ ½D2G 42 ... ½D2G 42 G8 02 G 42 G8 02 þ Â ½D2G 42 G8 02 G3* þ Â ½D2G 42 G8 02 G3*G3* þ Â ½D2G 42 G8 02 G3*G 42 þ Â ½D2G 42 G8 02 G3*G3*G 42 G8 02 þ Â ½D2G 42 G8 02 G3*G3*G 42 G8 02 G3* þ Â ½D2G 42 G8 02 G3*G3*G 42 G8 02 ... G3*G3* þ Â ½D2G 42 G8 02 G3*G 42 þ Â ½D2G 42 G8 02 G3*G3*G 42 þ Â ½D2G 42 G8 02 G3*G 42 G8 02 þ Â ½D2G 42 G8 02 G3*G3*G 42 G8 02 þ Â ½D2G 42 G8 02 G3*G3*G 42 G8 02 G3* þ Â ½D2G 42 G8 02 G3*G3*G 42 G8 02 G3*G3*...
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Báo cáo khoa học: Death inducer obliterator protein 1 in the context of DNA regulation Sequence analyses of distant homologues point to a novel functional role docx
Ngày tải lên :
07/03/2014, 21:20
... anaphase (arrowhead) FEBS Journal 27 2 (20 05) 3505–3511 ª 20 05 FEBS The complete protein sequences of human DIDO isoforms and were searched against PFAM [22 ,23 ] and SMART [24 ] databases to automatically ... EMBO J 20 , 7137–7148 11 Kennison JA (1995) The Polycomb and trithorax group proteins of Drosophila: trans-regulators of homeotic gene function Annu Rev Genet 29 , 28 9–303 FEBS Journal 27 2 (20 05) ... protein domains Nucleic Acids Res 26 , 320 – 322 23 Bateman A, Birney E, Cerruti L, Durbin R, Etwiller L, Eddy SR, Griffiths-Jones S, Howe KL, Marshall M & Sonnhammer EL (20 02) The Pfam protein families...
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Báo cáo khoa học: A mouse model for in vivo tracking of the major dust mite allergen Der p 2 after inhalation docx
Ngày tải lên :
07/03/2014, 21:20
... Aalberse RC (20 00) Structural biology of allergens J Allergy Clin Immunol 106, 22 8 23 8 Huby RD, Dearman RJ & Kimber I (20 00) Why are some proteins allergens? Toxicol Sci 55, 23 5 24 6 Pomes A (20 02) Intrinsic ... different time points (6, 24 and 48 h) and the radioactivity was tracked by whole-body autoradiography (Fig 2) The highest levels of radioactivity FEBS Journal 27 2 (20 05) 3449–3460 ª 20 05 FEBS In vivo ... separated liver and thoracic lymph node proteins revealed a radioactive band of 25 kDa in liver and bands of 30 and 56 kDa in thoracic lymph nodes (Fig 5C) No radioactive protein bands could be...
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Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
Ngày tải lên :
07/03/2014, 21:20
... protein and so far there is no biochemical evidence to support the proposition FEBS Journal 27 2 (20 05) 40 02 4010 ª 20 05 FEBS Tagged photosystem I We sought to determine the topology of PSI-G and ... placed in an Eppendorf tube and frozen in liquid nitrogen Frozen tissue was pulverized in 20 0 lL protein extraction buffer [PEB: 100 mm FEBS Journal 27 2 (20 05) 40 02 4010 ª 20 05 FEBS Tagged photosystem ... 567–580 19 Skerra A & Schmidt TGM (20 00) Use of the Strep-tag and streptavidin for recombinant protein purification and detection Methods Enzymol 326 , 27 1–304 20 Kay R, Shan A, Daly M & McPherson...
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Báo cáo khoa học: In vivo studies of altered expression patterns of p53 and proliferative control genes in chronic vitamin A deficiency and hypervitaminosis pot
Ngày tải lên :
08/03/2014, 08:20
... follows: 95 °C (2 min), four cycles at 92 °C (15 s), 50 °C (30 s) and 72 °C (2 min), 30 cycles at 92 °C (15 s), 60 °C (30 s), and 72 °C (2 min), and an additional extension step at 72 °C for Amplified ... sterile nuclease-free water, 3 .2 lL dNTP mix, lL T7 promoter 22 -mer primer (2 lM), lL M13 reverse 24 -mer primer (2 lM), 2. 4 lL MgCl2 (25 mM), lL AmpliTaq PCR Buffer (10 ·), and 0.4 lL AmpliTaq enzyme ... ND c 2. 88 ± 0.48 (6) b 64.66 ± 10.97(6) b 0.18 ± 0.03 (2) 5.60 ± 1.66 (4) c b ND 0.64 ± 0.11 (4) 2. 99 ± 0.48 (6) ND 0 .23 ± 0. 02 (3) 2. 99 ± 0. 32 (4) a b Results Physiological parameters and all-trans...
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Báo cáo Y học: In vivo activation of plasma membrane H+-ATPase hydrolytic activity by complex lipid-bound unsaturated fatty acids in Ustilago maydis docx
Ngày tải lên :
08/03/2014, 22:20
... 0.65 0.48 0.07 0.13 1.48 3. 82 1.10 2. 17 3 .21 5 .27 ± ± ± ± ± ± 0.39 0 .26 0 .21 0 .25 0.50 1.75 Ó FEBS 20 02 Stress activation of H+-ATPase by fatty acids (Eur J Biochem 26 9) 1009 Fig General scheme ... 0 .20 4.55 7. 92 6.57 3.60 3. 02 6.43 ± ± ± ± ± ± 0.16 0.44 0.37 0 .29 0.19 0.70 Erythrosin B Ki 5.58 4.74 3.94 6 .28 6.50 2. 89 ± ± ± ± ± ± 0.93 1. 02 0.31 2. 57 0.41 0.07 1.74 7.37 4.98 3.97 1.54 2. 58 ... PiÆmin)1Æmg)1 protein); Ki and aKi (lM); ± SE of estimation MgATP 18 : 2/ 18 : Ratio Et-C Tri-T Fen-T Wild-type A14 P51 Km 1.3 6.6 3.9 0.8 1.4 2. 0 2. 66 2. 00 2. 01 1.68 2. 53 2. 31 Vanadate aKi Vmax ±...
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Báo cáo khoa học: A study of microRNAs in silico and in vivo: bioimaging of microRNA biogenesis and regulation doc
Ngày tải lên :
16/03/2014, 01:20
... JM, Lee DS & Lee MC (20 05) Visualiza- FEBS Journal 27 6 (20 09) 21 65 21 74 ª 20 09 The Authors Journal compilation ª 20 09 FEBS S Kim et al 11 12 13 14 15 16 17 18 19 20 21 22 23 24 tion of endogenous ... decreased by mature miRNA 221 during the development of FEBS Journal 27 6 (20 09) 21 65 21 74 ª 20 09 The Authors Journal compilation ª 20 09 FEBS 21 69 Bioimaging of miRNA biogenesis and regulation S Kim ... the reporter gene is turned off [24 28 ] FEBS Journal 27 6 (20 09) 21 65 21 74 ª 20 09 The Authors Journal compilation ª 20 09 FEBS 21 67 Bioimaging of miRNA biogenesis and regulation S Kim et al pre-miRNA...
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Báo cáo khoa học: In vivo degradation of nitric oxide synthase (NOS) and heat shock protein 90 (HSP90) by calpain is modulated by the formation of a NOS–HSP90 heterocomplex pot
Ngày tải lên :
30/03/2014, 04:20
... tyrosine phosphorylation and interacts with caveolin-1 J Biol Chem 27 1, 27 237 27 240 FEBS Journal 27 5 (20 08) 25 01 25 11 ª 20 08 The Authors Journal compilation ª 20 08 FEBS 25 11 ... TJ (20 03) Involvement of the proteasome in activation of endothelial nitric oxide synthase Life Sci 73, 22 25 22 36 FEBS Journal 27 5 (20 08) 25 01 25 11 ª 20 08 The Authors Journal compilation ª 20 08 ... 99, 123 15– 123 20 15 Musial A & Eissa T (20 01) Inducible nitric-oxide synthase is regulated by the proteasome degradation pathway J Biol Chem 27 6, 24 268 24 273 16 Bender A, Demady DR & Osawa Y (20 00)...
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báo cáo hóa học:" In vivo properties of the proangiogenic peptide QK" pdf
Ngày tải lên :
18/06/2014, 15:20
... 20 04, 109 :25 87 -25 93 Sorriento D, Ciccarelli M, Santulli G, Campanile A, Altobelli GG, Cimini V, Galasso G, Astone D, Piscione F, Pastore L, Trimarco B, Iac- 18 19 20 21 22 23 24 25 26 27 28 29 ... (n = 12) , common carotids were suspended in isolated tissue baths filled with 25 mL Krebs-Henseleit solution (in mMol/L: NaCl 118.3, KCl 4.7, CaCl2 2. 5, MgSO4 1 .2, KH2PO4 1 .2, NaHCO3 25 , and glucose ... angiogenesis: structural characterization and biological properties of a de novo engineered VEGF mimicking peptide Proc Natl Acad Sci USA 20 05, 1 02: 1 421 5-1 422 0 Dudar GK, D'Andrea LD, Di Stasi R, Pedone C,...
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Báo cáo hóa học: " In vivo evidence of htid suppressive activity on ErbB-2 in breast cancers over expressing the receptor" potx
Ngày tải lên :
18/06/2014, 16:20
... (%) 1 32 63 76 54 29 3 28 5 114 165 HER -2 ΔCt 1,63 0,44 0 ,29 -0,11 0,60 -0,69 -1,11 -2, 37 ΔΔCt 3,95 2, 76 2, 61 2, 21 1, 72 1,63 1 ,21 -0,05 0,06 0,15 0,16 0 ,22 0,30 0, 32 0,43 1,04 15 16 22 30 32 43 104 ... 0,79 3,16 2, 27 52 135 197 24 64 79 316 22 7 1,55 2, 99 2, 21 2, 89 0,77 0, 82 1,88 0,35 T/N/M/G status Target gene htid-L 2- ΔΔCt Rel expression (%) ΔCt htid-I ΔΔCt 0,54 1 ,28 1 ,20 1,88 -0 ,24 -0,19 0,87 ... cancer Sample 2/ 1/1/3 1/0/0/3 4/1/x /2 3/1/1/3 2/ 1/1/3 2/ 1/1/3 1 /2/ 0 /2 2/1/0/3 ΔCt 4,68 3, 32 2,77 5,81 4,38 4.08 2, 08 2, 57 ΔΔCt 0,94 -0,43 -0,98 2, 07 0,64 0,34 -1,66 -1,18 0, 52 1,35 1,97 0 ,24 0,64 0,79...
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báo cáo hóa học:" Function of anterior talofibular and calcaneofibular ligaments during in-vivo motion of the ankle joint complex" docx
Ngày tải lên :
20/06/2014, 01:20
... 25 Wunderlich RE, Cavanagh PR: Gender differences in adult foot shape: implications for shoe design Med Sci Sports Exerc 20 01, 33(4):605-11 References 10 11 12 13 14 15 16 17 18 19 20 21 22 23 ... shortened from the neutral position at 28 .0 ± 2. 9 mm to 26 .6 ± 2. 2 mm at maximal plantarflexion (p = 0.08) and elongated significantly from the neutral position to 29 .9 ± 3.0 mm at maximal dorsiflexion ... environment of Materials and methods Subject recruitment Four ankles of healthy male subjects between 32 and 45 years of age (two left and two right, average height of 175 cm and average body...
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báo cáo hóa học:" In vivo evaluation of a vibration analysis technique for the per-operative monitoring of the fixation of hip prostheses" pdf
Ngày tải lên :
20/06/2014, 01:20
... 4:10 10 11 12 13 14 15 16 17 18 19 20 21 22 23 Søballe K, Hansen ES, Rassmussen HB, Jorgensen PH, Bunger C: Tissue ingrowth into titanium and hydroxyapatite-coated implants during stable and unstable ... Suceava Edited by: Dumitru I Suceava: University of Suceava; 20 05:505-510 http://www.josr-online.com/content/4/1/10 24 25 26 27 28 29 30 31 32 Pastrav LC, Jaecques SVN, Mulier M, Va der Perre G: Detection ... Prediction and evaluation of total hip replacement performance: can we plan success? 22 23 June 20 07; Leuven Edited by: Jonkers I, Jaecques S, Mulier M, Van der Perre G Leuven: K.U Leuven; 20 07 :28 -30...
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