2 h to set up on day of experiment
Báo cáo y học: "TNF inhibits production of stromal cell-derived factor 1 by bone stromal cells and increases osteoclast precursor mobilization from bone marrow to peripheral blood" ppt
... expression on target cells Although alternation of either of these could lead to impaired cell mobilization and homing, external factor regulation of SDF1 expression levels appears to be the major ... and that TNF promotes the release of bone marrow OCPs into the bloodstream [3-5] To investigate whether the SDF-1/CXCR4 axis – the master chemokine system controlling mobilization of hematopoietic ... arthritis, in that the entire process of generation of OCPs and their egress from the bone marrow is accelerated in response to TNF This situation leads to increased numbers of OCPs in both bone...
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Báo cáo y học: "Pro-inflammatory properties of stromal cell-derived factor-1 (CXCL12) in collagen-induced arthritis" pptx
... destruction [5] Availability of specific inhibitors of the CXCL 12- CXCR4 interaction has allowed the demonstration of the involvement of CXCL 12 in experimental animal diseases One such inhibitor is the ... CXCR4/CXCL 12 chemotactic interaction during Available online http://arthritis-research.com/content/7/6/R 120 8 20 21 22 23 24 25 26 hematopoietic stem cell mobilization induced by GCSF or cyclophosphamide ... response to the chemokine CXCL 12 Challenging mice with CXCL 12 while they were treated with AMD3100 reduced the number of harvested cells to that of the negative control The number of cells in the air...
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Báo cáo khoa học: Syndecan-4 is a signaling molecule for stromal cell-derived factor-1 (SDF-1)/ CXCL12 pptx
... incubation with the chemokine was enhanced from to 15 On the contrary, these phosphorylations decreased if the time of incubation with the chemokine was enhanced up to 30 (Fig 6A) According to these results, ... 1937–1951 ª 20 05 FEBS N Charnaux et al 19 20 21 22 23 24 25 26 27 28 29 immunodeficiency virus type infectivity J Virol 76, 22 45 22 54 Oh ES, Woods A & Couchman JR (1997) Multimerization of the cytoplasmic ... stimulation of HeLa cells This tyrosine phosphorylation depends on the time of incubation of the cells with the chemokine: marginal for 2- min incubation, significant FEBS Journal 27 2 (20 05) 1937–1951...
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Báo cáo sinh học: "Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral blood lymphocytes in vitro" pot
... the CD3 ζ (zeta) chain component of the T cell receptor Addition of the part of the small intracellular domain of the co-stimulatory receptor molecule CD28 has been shown to improve the responses ... X-tremeGENE, Roche) The focus was then changed to retroviral vectors for introduction of the chTCR cassette into the human genome Retroviruses have the unique advantage of integration into all host cell ... phosphorylation of tyrosine residues in its cytoplasmic domain The cytoplasmic tail of CD28 is very short (~40 amino acids) and has only four tyrosines, only one of which (tyrosine 188 of the mouse...
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báo cáo hóa học:" Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral blood lymphocytes in vitro" docx
... the CD3 ζ (zeta) chain component of the T cell receptor Addition of the part of the small intracellular domain of the co-stimulatory receptor molecule CD28 has been shown to improve the responses ... X-tremeGENE, Roche) The focus was then changed to retroviral vectors for introduction of the chTCR cassette into the human genome Retroviruses have the unique advantage of integration into all host cell ... phosphorylation of tyrosine residues in its cytoplasmic domain The cytoplasmic tail of CD28 is very short (~40 amino acids) and has only four tyrosines, only one of which (tyrosine 188 of the mouse...
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Báo cáo y học: "Mesenchymal stem cell derived hematopoietic cells are permissive to HIV-1 infection" pptx
... Morphological changes in differentiated HD cells following exposure to HIV-1 Day- 0, HD cells which are on day th of differentiation and before viral infection Day- 3, shows the morphological changes of HD ... growth arrest conditions as a result of HIV-1 infection [39] HIV-1 has also been shown to regulate the expression of CDK9 [40] It has been shown that STAT3 promotes the initiation of transcription ... levels of p24 on days to On day of infection, p24 levels in 5-HD and 8-HD cultures remained unchanged; the p24 levels in ASCs-HIV were negligible, indicating no evidence of viral replication To quantify...
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Cloning of medaka glial cell derived neurotrophic factor (GDNF) and its receptor GFR alpha 1
... Gfrα1 contains 10 exons (Table 3) and like its ligand, there are no splicing variants On the other hand, human GFRα1 has two isoforms; the longer isoform has 11 exons while the shorter one has one ... further divide into chains of 8, 16 and up to, although rarely, 32 cells Upon reaching this stage, the spermatogonia will give rise to more differentiated germ cells such as A1–A4 spermatogonia, ... factor receptor substrate 2) while those that moves to the outside of the rafts trigger the signal through SHC (Src-homologous and collagen-like protein) (Fig 3B) Since SHC and FRS2 share the...
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báo cáo hóa học:" Anti-tumor activity of patient-derived NK cells after cell-based immunotherapy – a case report" doc
... another 12 months after the therapy had been terminated A scheme of the therapeutic approach and the course of the disease are summarized in Figure Identical to the protocol of the clinical phase ... The expression of CD25 was considered here as a marker of lymphocyte activation since it did not show any correlation with the amount of CD4+ cells which would reflect the presence of regulatory ... and the patient's written informed consent In contrast to the phase I clinical trial, the whole procedure was repeated up to times on a monthly rather than a 2- weekly basis After a 3-month treatment...
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Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx
... http://www.translational-medicine.com/content/9/1/90 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 and prime the differentiation of CD4+CD25+Foxp3+ regulatory T cells J Immunol 20 09, 1 82: 2795 -28 07 ... difficult to inhibit for cancer therapy A better therapeutic approach, then, is likely to evolve from inhibition of the transcription factors promoting the suppressive phenotype Here we showed that HIF1a ... NCI -H 125 1.54 3.69 * Panc 2. 03 22 .28 4.37 0 .28 2. 89 * Panc 4.14 Panc 9.6.94 21 . 82 27 .28 2. 60 8.47 ** NCI -H4 64 63.96 6.00 Panc-1 7. 82 3.69 * NCI -H6 0 47.79 7.71 Panc 3 .27 6.98 5.44 NCI-HUT 69C -24 .28 ...
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Báo cáo sinh học: "Autologous Transplantation of Adipose-Derived Mesenchymal Stem Cells Markedly Reduced Acute Ischemia-Reperfusion Lung Injury in a Rodent Model" potx
... between group and group On the other hand, an increase of cytochrome c in cytosol (Figure 7C), an index of mitochondrial damage, was notably higher in group than that in groups and However, no ... in group than in groups and 3, and notably higher in group than in group at 72 h after the IR procedure (Figure 1A) On the other hand, RVSBP was notably lower in groups and than in group 2, and ... significantly higher in group than that in group Besides, mitochondrial cytochrome c (Figure 7B), an index of mitochondrial integrity, was notably reduced in group compared with that in groups and...
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Báo cáo khoa học: "Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury" potx
... Transplantation of ASCs in spinal cord injury 28 3 12 13 14 15 16 17 18 19 20 21 22 23 24 25 isolation, characterization, and differentiation potential Cytotherapy 20 03, 5, 3 62- 369 Horky LL, Galimi ... Mareci TH Evaluation of the pathologic characteristics of excitotoxic spinal cord injury with MR imaging AJNR Am J Neuroradiol 20 05, 26 , 16 12- 1 622 Boquest AC, Shahdadfar A, Frønsdal K, Sigurjonsson ... lesions However, they were not sufficient to show changes of spinal cord lesions in the chronic phase In vivo MSC studies have demonstrated the cellular fate of cells which integrated into injured...
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Báo cáo khoa học: "Regeneration of human bones in hip osteonecrosis and human cartilage in knee osteoarthritis with autologous adipose-tissue-derived stem cells: a case series" docx
... pain due to osteoarthritis Due to her occupation, she made active use of her bilateral knee joints With a diagnosis of osteoarthritis of the right knee, she had received multiple injections of steroids ... pain due to osteoarthritis Her left knee was much more painful than the right Due to her occupation, she made active use of her bilateral knee joints With a diagnosis of osteoarthritis of Pak Journal ... week 12 showed a significant filling of bone defects on the superior acetabulum and probable bone matrix formation in the subcortical region of the femoral head (Figure 1) The second case concerns...
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Báo cáo y học: " Alteration of T cell immunity by lentiviral transduction of human monocyte-derived dendritic cells" pdf
... Benedict SH: Stimulation through intercellular adhesion mol- 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 ecule-1 provides a second signal for T cell activation J Immunol 20 02, 168:5530-5537 Salomon ... has been shown to enhance IL 12 production in DCs [30] To overcome the impaired Th1 response after LV transduction, we investigated whether modification of the local cytokine environment in the ... response is critical for polarization of CD4+ T cells toward Th1 function, which is essential for the clearance of intracellular pathogens IL10, on the other hand, suppresses IL- 12 production...
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Báo cáo y học: "Transcriptomic and phenotypic analysis of murine embryonic stem cell derived BMP2+ lineage cells: an insight into mesodermal patterning" ppt
... S, Hattori A, Sato M, Tsujimoto M, Kohno M: Characterization of the bone morphogenetic protein -2 as a neurotrophic factor Induction of neuronal differentiation of PC 12 cells in the absence of ... depending upon the stimuli This is further confirmed by the upregulated expression of leukemia inhibitory factor (LIF) in the BMP2+ population compared to the control EBs Interestingly, the transcripts ... control EB BMP2+ 140 Relative expresion 20 µm 120 100 80 60 40 20 Calponin SM 22- alpha SMA Detection of smooth muscle cells after differentiation of the BMP2+ cells Figure Detection of smooth...
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Báo cáo y học: "Analysis of proteomic profiles and functional properties of human peripheral blood myeloid dendritic cells, monocyte-derived dendritic cells and the dendritic cell-like KG-1 cells reveals distinct characteristic" doc
... 1,8, 12, 13 24 .41 21 .01 5.94/ 52, 618.79 13 58 1 ,2 - - - - 8.05 -2. 27 - Not in mo 21 .06 Glutathione S-transferase P P0 921 1 - 2. 49 1. 42 5.44 /23 ,22 4.64 L-lactate dehydrogenase B chain 40 1 02 1, 2, P07195 ... MA, Thomson JA, Gumenyuk ME, Choi KD: Directed differentiation of human embryonic stem cells into functional dendritic cells through the myeloid pathway J Immunol 20 06, 176 :29 24 -29 32 Ghaemmaghami ... 8:R30 http://genomebiology.com /20 07/8/3/R30 18 19 21 22 24 26 27 29 30 31 33 34 36 Genome Biology 20 07, 8:R30 information 35 interactions 32 refereed research 28 deposited research 25 reports 23 Horlock...
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Use of IPs cell derived neural stem cells as a cellular vehicle for glioma and breast cancer therapy
... technical help, tips and advices but also the camaraderie I am grateful for my family, especially my husband and son who have been with me through the tough times They have been nothing but supportive ... 3 .2. 4 Characterization of msiPSC-NSC 37 3 .2. 5 RT2 profiler PCR array 38 3 .2. 6 Baculoviral vector construction and transduction 40 3 .2. 7 Cytotoxicity assay 40 3 .2. 8 In vitro migration assay 3 .2. 9 ... cells In addition, the construction, propagation and scaling up of baculovirus can be performed easily (Hu YC 20 08) 18 1.3 .2 Synthetic vectors The limitations of viral vectors, their relatively...
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Gene targeting in human pluripotent cell derived neural stem cells for the study and treatment of neurological disorders
... Adjust pH to 7.0 with concentrated HCl, then add H2 O to 1000 ml 37 20 x SSC Transfer Buffer 175.5 g NaCl 88 .2 g Na citrate add H2 O to 800 ml Adjust pH to 7.0 with concentrated HCl, then add H2 O to 1000 ... adenosine action (Dunwiddie and Masino, 20 01; Fredholm et al., 20 05b) The anticonvulsant functions of adenosine are thought to base largely upon the activation of A1 receptors coupled to inhibitory G ... Adjust pH to 7.5 with solid NaOH Southern detection buffer 15.76 g Tris-HCl 5.84 g NaCl add H2 O to 1000 ml Adjust pH to 9.5 with solid NaOH TE-buffer 1.58 g Tris-HCl 0 .29 g EDTA add H2 O to 1000...
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Tài liệu Báo cáo Y học: Uncoupling of protein-3 induces an uncontrolled uncoupling of mitochondria after expression in muscle derived L6 cells ppt
... analysis has shown that only nonphysiological, high concentrations of UCP2 and UCP3 induced the changes in the mitochondrial proton permeability previously observed These uncoupling effects were the ... the addition of the mitochondrial H+ -ATPase inhibitor oligomycin strongly reduced the oxygen consumption As the oligomycin-resistant portion of respiration reflects the level of proton leak of ... function is to increase the mitochondrial proton leak The role of UCP3 in the control of energy homeostasis became apparent after the generation of mice with a disrupted UCP3 gene [20 ,21 ] The knock-out...
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