2 department of medicine national university of singapore singapore 119074
Engineering of an efficient and enantioselevtive biocatalyst for the preparation of chiral pharmaceutical intermediates
... 31 2. 1 Introduction 31 2. 2 Two-Enzyme-Based Colorimetric ee Screening Assay 33 2. 2.1 Results and Discussion 33 2. 2 .2 Conclusion and Outlook 38 2. 3 ... organization of Class I P450s 28 Figure 2. 1 SDS-PAGE of purified N-histag BRD and N-histag RDR 35 Figure 2. 2 Codon optimized sequence of the RDR gene 36 Figure 2. 3 Graph shows ... SDS-PAGE of non-induced R12x control (lane 1), Rgdh (lane 2) , R12x (lane 3) and A2 (lane 4) 95 Figure 4.3 GDH cofactor regeneration system 99 Figure 4.4 PTDH 12x cofactor...
Ngày tải lên: 12/09/2015, 10:22
Tài liệu Báo cáo khoa học: Medium-chain dehydrogenases/reductases (MDR) Family characterizations including genome comparisons and active site modelling pdf
... 8.0 7.5 6.7 8.5 5.9 21 2 173 144 25 7 21 1 22 6 24 4 21 0 21 0 22 6 22 6 176 20 7 20 7 21 2 20 5 20 2 145 188 77 )130 CSIHN-HEVVEIFRLN 0.05 16.0 6.8 161 )133 CSRAH-HEFSEVTWMN )0.71 12. 4 8.6 22 0 Cinnamyl-alcohol ... SC_YBR 026 C CE_Y48A6B_9 AT_T6D9–100 DM_730 326 0 CE_W09H1–5 HS_ENSP234985 LTDe AT_F2K13–110 AT_F2K13–140 AT_F2K13–150 AT_F2K13– 120 AT_F2K13–130 AT_T17B 22 23 AT_F28B23–3 AT_K18L3–100 AT_k19a23–10 AT_F24G16–110 ... )28 )34 ) 121 ) 52 )16 )9 NADLQYLALGETLFLSR NSDNYYF-YPVTFLAQG NSDNFYF-VFTTMLQAG 0 .22 )0 .21 )0.41 )0.43 0 .21 )0.35 0.13 17.0 18.5 12. 5 22 .0 11 .2 18.4 17.6 7.1 6.7 8.7 7.3 8.9 6.7 7.0 20 1 22 0 124 ...
Ngày tải lên: 21/02/2014, 03:20
Báo cáo khoa học: The lactate dehydrogenases encoded by the ldh and ldhB genes in Lactococcus lactis exhibit distinct regulation and catalytic properties ) comparative modeling to probe the molecular basis pdf
... dehydrogenases of Lactococcus lactis 16 17 18 19 20 21 22 23 24 25 26 27 28 of different NADH oxidase levels on glucose metabolism by Lactococcus lactis: kinetics of intracellular metabolite pools determined ... of NADH independently of pH The Km of LDHB for NADH increased substantially FEBS Journal 27 4 (20 07) 5 924 –5936 ª 20 07 The Authors Journal compilation ª 20 07 FEBS 5 925 Lactacte dehydrogenases of ... FEBS Journal 27 4 (20 07) 5 924 –5936 ª 20 07 The Authors Journal compilation ª 20 07 FEBS 5 929 Lactacte dehydrogenases of Lactococcus lactis P Gaspar et al LDHB LDH pH pH -20 kT/e interfaces 20 kT/e A-B...
Ngày tải lên: 16/03/2014, 05:20
Báo cáo khoa học: High and complementary expression patterns of alcohol and aldehyde dehydrogenases in the gastrointestinal tract Implications for Parkinson’s disease docx
... kinetic FEBS Journal 27 4 (20 07) 121 2– 122 3 ª 20 07 The Authors Journal compilation ª 20 07 FEBS 122 1 Expression of ADH and ALDH in the rodent GI tract 17 18 19 20 21 22 23 24 25 26 27 28 29 30 M Westerlund ... proliferation [26 ] Expression of Adh1 in deeper layers of the FEBS Journal 27 4 (20 07) 121 2– 122 3 ª 20 07 The Authors Journal compilation ª 20 07 FEBS M Westerlund et al Fig Schematic drawings of the stomach ... mucosal layer of the rodent GI tract, with characteristic regional differ- FEBS Journal 27 4 (20 07) 121 2– 122 3 ª 20 07 The Authors Journal compilation ª 20 07 FEBS 121 9 Expression of ADH and ALDH...
Ngày tải lên: 16/03/2014, 11:20
Báo cáo khoa học: Prediction of coenzyme specificity in dehydrogenases ⁄ reductases A hidden Markov model-based method and its application on complete genomes doc
... Total 26 38 31 95 10 13 25 4793 4769 4774 4694 0. 929 0.7 92 0.705 0.7 92 1.000 0.999 0.999 0.999 4 821 4 821 4 821 4 821 FEBS Journal 27 3 (20 06) 1177–1184 ª 20 06 The Authors Journal compilation ª 20 06 ... 4 92 105 5 3 2 18 15 12 10 17 14 12 12 12 8 10 17 10 11 13 10 11 5 8 11 82 74 72 49 45 45 40 30 FEBS Journal 27 3 (20 06) 1177–1184 ª 20 06 The Authors Journal compilation ª 20 06 FEBS Y Kallberg ... irrespective of the coenzyme binding site location The numbers include all 68 investigated genomes Coenzyme 1N TM 2N TM 1C TM 2C TM >2 TM All FAD NAD NADP Dual Total 25 71 100 17 21 3 27 17 57 87 115 142...
Ngày tải lên: 23/03/2014, 10:21
Báo cáo khóa học: Acyl-CoA dehydrogenases and acyl-CoA oxidases Structural basis for mechanistic similarities and differences pot
... Molecular cloning and expression of cDNA 16 17 18 19 20 21 22 23 24 25 26 27 28 29 encoding 3a,7a,12a-trihydroxy-5b-cholestanoyl-CoA oxidase from rabbit liver J Biol Chem 27 2, 18481–18489 Zhang, Y.X., ... & Kim, J.J (20 02) Crystal structure of rat short chain acyl-CoA dehydrogenase complexed with acetoacetyl-CoA: comparison with other acyl-CoA dehydrogenases J Biol Chem 27 7, 122 00– 122 07 Kim, J.J.P., ... carried out with i3VD [24 ], SCAD [27 ], and i2VD [29 ], demonstrating that the catalytic residues, Glu254 in i3VD, Glu368 in SCAD, and Glu381 in i2VD are homologs of Glu254 in LCAD and Glu376 in...
Ngày tải lên: 23/03/2014, 12:20
Báo cáo khóa học: Genetic defects in fatty acid b-oxidation and acyl-CoA dehydrogenases Molecular pathogenesis and genotype–phenotype relationships ppt
... 5.3 54.0 45.5 38.0 45.5 13 15q23 19q13 4q 32 5q33 3p21 11q13 22 q13 17p11 2p23 2p23 1p31 4q 22 12q 22 CAT SLC22A5 CACT SLC25A2 CPTI CPT1A CPTII CPT2 VLCAD ACADVL LCHAD HADHA LCKAT HADHB MCAD ACADM ... [55] 12 – 34 – – – – – – – – – – – – – – 20 1 – – – – – – – – – 1.7 0.9 2. 3 2. 0 2. 0 2. 3 1.4 1.3 0.9 1 .2 10 18 20 20 16 12 10 25 .8 16.5 [81] 60.03 17.8 5.3 54.0 45.5 38.0 45.5 13 15q23 19q13 4q 32 ... Dewar, K., Doyle, M., FitzHugh, W., 17 18 19 20 21 22 23 24 25 et al (20 01) Initial sequencing and analysis of the human genome Nature 409, 860– 921 Venter, J.C., Adams, M.D., Myers, E.W., Li,...
Ngày tải lên: 23/03/2014, 12:20
Báo cáo khoa học: Inhibition kinetics of catabolic dehydrogenases by elevated moieties of ATP and ADP – implication for a new regulation mechanism in Lactococcus lactis potx
... Goodness of t KM 0. 32 0 .23 0. 32 0.13 0.58 0.16 0.30 0.69 0.39 0.38 0.062a 0.062a 0.062a 0.06 0. 02 0.06 0.03 0.06a 0.06a 0.14 0. 02 0.14a 0.14a 15.6 24 .4 0.08 27 .7 7.0 28 .4 23 .1 0.06 0 .21 ... AMP 2. 55 1.90 1.56 1.64 4.61 1.45 3.43 2. 03 0.96 0 .27 KIU 0.54 0 .20 1.48 0.51 0.34 0 .20 0.78 0.36 0 .26 0 .26 n 4.16 0. 92 5.38 2. 76 5 .21 5.69 1.15 1.75 0.94 0.94 4. 02 1. 42 1.55 ... 0.9 920 0.9808 0.9888 0.3743 0.5449 0.0 020 0.6533 0 .22 27 0.6 720 0.88 92 0.0015 0.0091 0.0015 Set as a xed value as determined in one of the other assays FEBS Journal 27 7 (20 10) 184318 52 ê 20 10...
Ngày tải lên: 29/03/2014, 08:20
Báo cáo hóa học: " Genetic variation and recombination of RdRp and HSP 70h genes of Citrus tristeza virus isolates from orange trees showing symptoms of citrus sudden death disease" potx
... not for citation purposes) Virology Journal 20 08, 5:9 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 Román MP, Cambra M, Juarez J, Moreno P, Vila ND, Tanaka FAO, Alves E, Kitajima ... (Y18 420 ) US (AF260651) R1b C R2 R3 US (U16304) US (NC 001661) Egypt (AY340974) Mexico (DQ2 725 79) Spain (AY170468) R4 % of population 100 80 60 40 20 26 5 20 29 26 90 15 44 31 C3 C5 C6 0.05 Figure ... breakpoints AIDS Res Hum Retroviruses 20 05, 21 :98-1 02 Smith JM: Analyzing the mosaic structure of genes J Mol Evol 19 92, 34: 126 - 129 Posada D, Crandall KA: Evaluation of methods for detecting recombination...
Ngày tải lên: 20/06/2014, 01:20
Báo cáo lâm nghiệp: "Changing electrophoretic patterns of glutamate dehydrogenases and aspartate aminotransferases in a few tree species under the influence of ectomycorrhization" potx
... Biochem 26 , 17 -28 Kusnan M.B., Berger M.G & Fock H.P (1987) The involvement of glutamine synthetase/glutamate synthase in ammonia assimilation by Aspergillus nidulans J Gen Microbiol 123 , 123 5- 124 2 ... plants Nature 25 1, 614-616 References Blumenthal K.H & Smith E.L (1973) Nicotinamide adenine dinucleotide phosphate-specific glutamate dehydrogenase of Neurospora J Biol Chem 24 8, 60 02- 6008 Martin ... ectomycorrhizas of spruce (Picea excelsa L.) and beech (Fagus sylvatica L.) New Phytol 111, 683-6 92 Khalid A., Boukroute A., Botton B & Martin F (1988) The aspartate aminotransferase of the ectomycorrhizal...
Ngày tải lên: 09/08/2014, 04:20
KINETIC ANALYSIS OF PRIMATE AND ANCESTRAL ALCOHOL DEHYDROGENASES
... -2- hexen-1-ol ~ Cyclohexanol 0.0 12 ββ 1 ,2 0.05 0.019 0.0 12 0.019 0. 022 ~ 3.9 γγ σσ 0.33 ~ 0.04 0. 02 ~ ~ 0.063 28 1.4 0.79 0 .28 0.13 0.018 ~ χχ ππ4 * ~ ~ 22 8 .2 ~ # 120 ~ ~ 0.09 ~ ~ 21 0 Table 2: ... Trans -2- hexen-1-ol Cyclohexanol 4B 22 B 2M 10M 30 29 14 17 21 460 0 .24 1 .2 10 15 15 32 480 4.5 29 23 24 26 21 350 30 38 90 110 100 470 330 (All data was rounded to significant figures.) 2M and ... Hexanol Trans -2- hexen-1-ol Cyclohexanol 4B 22 B 2M 10M 17 +/- 18 +/- 15 +/- 12 +/- 9.8 +/- 0 .2 18 +/- 9.7 +/- 1.9 12 +/- 12 +/- 12 +/- 12 +/- 10 +/- 18 +/- 9.8 +/- 0.8 23 +/- 22 +/- 29 +/- 16 +/-...
Ngày tải lên: 24/08/2014, 12:25
TRANSCRIPTIONAL REGULATION OF THE HUMAN ALCOHOL DEHYDROGENASES AND ALCOHOLISM
... 20 Alcohol and the liver 21 10 Alternative transcript isoforms and diseases 24 11 Global transcriptional profiling 27 12 Research objectives 32 viii ... 54 Characterization of the enhancer element 4E 58 2. a Effect of 4E on heterologous promoters 58 2. b Function of 4E in non-hepatoma cells 58 2. c Localization of sequences required ... exon array data 111 Figure 20 Examples of different groups of alternatively spliced genes 114 Figure 21 Detection of alternative isoforms for validation 129 xiii ABBREVIATIONS µg microgram...
Ngày tải lên: 24/08/2014, 12:37
EMBEDDED LINUX SYSTEM DESIGN AND DEVELOPMENT.pdf
... (HAL) 33 2. 2 .2 Memory Manager 33 2. 2.3 Scheduler 34 2. 2.4 File System 35 2. 2.5 IO Subsystem 36 2. 2.6 Networking Subsystems 36 2. 2.7 IPC 36 2. 3 User ... Architecture of Embedded Linux 29 2. 1.1 Real-Time Executive 29 2. 1 .2 Monolithic Kernels 30 2. 1.3 Microkernel 31 2. 2 Linux Kernel Architecture 32 2 .2. 1 Hardware ... 20 0 Contents xiii Real-Time Linux 20 1 7.1 Real-Time Operating System 20 2 7 .2 Linux and Real-Time 20 2 7 .2. 1 Interrupt Latency 20 3 7 .2. 2 ISR Duration 20 4...
Ngày tải lên: 04/08/2012, 14:23
09 Physical and Chemical Characteristics of DDGS revisions.
... Mean 1398 1 322 1 425 1370 125 5 6 12 974 125 8 11 42 1337 1488 123 5 1198 122 9 21 25 1148 128 2 .25 Standard Deviation 2. 32 2.00 1. 62 1.84 1.68 2. 75 2. 15 1.70 1.84 1.78 1. 62 1.75 1.87 2. 09 1.56 2. 25 1.93 ... will fall between: 603 324 3 661 26 44 880 23 09 745 25 21 747 21 08 22 3 1683 453 20 94 740 21 39 621 21 01 751 23 80 919 24 11 706 21 61 641 22 40 588 25 69 13 62 3315 510 25 83 697 24 06 Two additional DDGS ... 10.70 10.71 10.76 10.93 11. 02 11 .28 11.16 11.70 11.88 12. 13 12. 26 27 .60 27 .61 27 .59 27 .63 27 . 62 27.73 27 .71 27 . 62 27.54 27 .61 27 .63 27 .61 27 .50 27 .53 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00...
Ngày tải lên: 08/08/2012, 10:03
CHECK YOUR ENGLISH VOCABULARY FOR BUSINESS AND ADMINISTRATION.pdf
... Dictionary of Business 4th edition (A&C Black 978-0-713-67918 -2) 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 42 41 43 45 44 46 47 48 49 50 51 52 53 ... 10 11 – 12 13 – 14 15 16 – 17 18 – 19 20 – 22 23 – 24 25 – 27 28 29 – 30 31 32 – 34 35 36 – 38 39 40 – 41 42 – 43 44 – 45 46 47 – 48 49 – 50 51 – 52 53 – 54 55 – 56 57 – 58 59 – 60 61 62 – 64 65 ... p y e t R s management Money a n e i g Offshore b n i g Foreign u r n y V l e added tax Net p r t n income 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 Operating r f t Interest r t B d...
Ngày tải lên: 08/08/2012, 17:31
Check Your English Vocabulary for Phrasal Verbs and Idioms.pdf
... 12 13 – 14 15 – 16 17 – 18 19 – 20 21 – 22 23 – 24 25 26 – 27 28 29 – 30 31 – 32 33 – 34 35 – 36 37 – 38 39 40 41 – 42 43 44 – 45 46 47 48 – 49 50 – 51 52 – 53 54 55 – 56 57 58 – 59 60 – 61 62 ... really smart _ 23 Brenda's new boyfriend is very good looking, but he's not very intelligent or interesting He's just a bit of eye _ 10 11 12 13 14 16 19 17 18 20 21 22 23 24 25 26 22 15 Unit 'get' ... I I 21 P I 22 24 25 T O 26 28 30 A E 29 O U Y 20 R D L 27 E Z 31 32 Y N I 23 H 19 G 20 17 E Idioms and other expressions using food and drink Complete these sentences with items of food or drink,...
Ngày tải lên: 08/08/2012, 17:32
Atmel AVR Microcontroller Primer Programming and Interfaceing
... PA2 (ADC2)-38 PA3 (ADC3)-37 PA4 (ADC4)-36 PA5 (ADC5)-35 PA6 (ADC6)-34 PA7 (ADC7)-33 AREF- 32 AGND-31 AVCC-30 PC7 (TOSC2) -29 PC6 (TOSC1) -28 PC5 -27 PC4 -26 PC3 -25 PC2 -24 PC1 -23 PC0 -22 PD7 (OC2) -21 ... tristate LED indicator 20 PD6 to tristate LED indicator 21 PD7 to tristate LED indicator 22 PC0 23 PC1 24 PC2 25 PC3 26 PC4 27 PC5 28 PC6 29 PC7 30 AVcc to VDD 31 AGnd to Ground 32 ARef to Vcc 33 PA7 ... 26 2. 2.3 Full Duplex 26 2. 2.4 Nonreturn to Zero Coding Format 26 2. 2.5 The RS -23 2 Communication...
Ngày tải lên: 16/08/2012, 09:03
Pro WPF and Silverlight MVVM effective Application Development
... Account 21 1 ViewModel and Tests 22 0 MainWindowViewModel 22 0 View 22 8 Summary 24 1 ■Index 24 3 ix ■ CONTENTS About ... Figure 2 2 Figure 2 2 UML2 component diagram showing a two-tier architecture of WPF/Silverlight and XML 27 CHAPTER ■ DATABINDING This architecture has little scope of business logic outside of the ... resulting PropertyMetaData has a number of properties that apply to this specific use of the DependencyProperty (see Table 2 2) 22 CHAPTER ■ DATABINDING Table 2 2 PropertyMetadata Properties Property...
Ngày tải lên: 17/08/2012, 08:54