2€ generating class files for core data entities

... 649 Figure 13-1 Creating an empty application for Core Data Now for your product name choose Introduction to Core Data and make sure the User Core Data check box is ticked, as shown in Figure ... buffer into an NSData */ NSData *readData = [[NSData alloc] initWithBytes:(const void *)buffer length:bytesRead]; /* And write the data to file */ [fileHandle writeData:readData]; } while (bytesRead ... a Core Data application to understand this a bit better Core Data in an iOS application needs a bit of setting up Fortunately, with Xcode, this process is very easy You can simply create a Core...

Ngày tải lên: 13/08/2014, 18:20

... Create an object of type EKEvent (for more information, refer to Recipe 14.2) Set the appropriate values for the event, such as its startDate and endDate (for more information, refer to Recipe 14.2) ... use the fontNamesForFamilyName: class method of the UIFont class, and get back an array of font names for the family name that we pass as a parameter: - (void) enumerateFonts{ for (NSString *familyName ... fontWithName:size: class method of the UIFont class: UIFont *helveticaBold = [UIFont fontWithName:@"HelveticaNeue-Bold" size:12.0f]; If the result of the fontWithName:size: class method of the UIFont class...

Ngày tải lên: 13/08/2014, 18:20

... (A) Plate assay for the utilization of oleic acid Yeast mls1D cells expressing Mls1p in its native form or without SKL were compared with an otherwise isogenic null mutant for formation of clear ... Determination of protein concentration was performed as described [29] Puri®cation of tagged Mls1p and generation of anti-Mls1p Ig To obtain pure protein for generating an antibody against Mls1p, the ... cycle is attributed to the cytosolic isoform Mdh2p [7] The suggestion of an extra-peroxisomal location for the yeast glyoxylate cycle was further reinforced by the demonstration that Icl1p is...

Ngày tải lên: 22/02/2014, 04:20

... more appealing approach to infer functions [5,6] when data extracted from the whole protein are limited or inadequately informative We therefore conducted domain analyses to obtain new insights ... tools) To obtain further information regarding these regions, we thus surveyed for the existence of distant DIDO protein homologues, using these regions as queries for extensive searches The ... e-values of 2e-30, 1e-10 and 1e-08 for DATF-1, PHF3 and CGBP, respectively Given the broad distribution and functional repertoire for the PHD domain in the databases, we studied its evolution...

Ngày tải lên: 07/03/2014, 21:20

... pathway for repair The XRCC1 component of the XRCC1–DNA ligase IIIa heterodimer is thought to be a protein providing such a mechanism by acting as a nick sensor and a docking platform for formation ... 5753–5763 ª 2005 FEBS Acknowledgements We thank Dr Keith Caldecott for critical reading of the manuscript and for communicating his data before publication 5761 Mammalian DNA single-strand break repair ... incubated with mock immunodepleted or PNK-immunodepleted HeLa cell extract (0–5 lg) for 20 before the addition of formamide loading dye, and the DNA separated on a 6% acrylamide gel and analysed...

Ngày tải lên: 23/03/2014, 11:20

... superimposed over the backbone atoms The RMSD ˚ for the backbone atoms of the b-sheet part is 0.72 A, indicating nearly identical conformation for the solution and crystal state in this region ... neurotoxins (a-BTX, a-CTX, toxin b and LSIII) and allows it to have more conformational diversity when searching for a suitable conformer in the binding cleft On the other hand a large flexibility at ... structure using 13C chemical-shift data J Biomol NMR 4, 171– 180 Cornilescu, G., Delaglio, F & Bax, A (1999) Protein backbone angle restraints from searching a database for chemical shift and sequence...

Ngày tải lên: 23/03/2014, 13:20

... Furthermore, it provides a rationale for why a precise sequence of administration of different vectors for priming or boosting the immune response is a crucial pre-requisite for an enhanced specific T ... Pagarigan, Angeline Quach, Darlene Rosario, and Victor Tam for generating some of the key experimental evidence in support of the model put forward in this review 21 22 Authors’ contributions AB ... summary References 1B Clinical trials Boosting vectors Vector category Targets / Formulations Proteins Polyvalent HIV Env formulation* Multivalent humoral and polyfunctional cellular immunity in healthy...

Ngày tải lên: 18/06/2014, 16:20

... explored in this class Changing Nature of Business Today  Intensifying competition  Increased knowledge & expertise among business people  Changing expectations Approach for Business Today ... • Engage in bargaining for win/win outcomes • Involve individuals or groups  Is pervasive throughout one’s life Focus of Text  How successful negotiation alliances are formed  Emphasizes language ... organizations • Collaborative 2-way communication • More business with fewer suppliers Skills necessary for business  “Key skills will be the ability to win friends and influence people at a personal...

Ngày tải lên: 31/07/2014, 17:20

... consensus sequence (B) For antibody interference assays, nuclear extracts were preincubated with specific antibody for Egr-1 (egr) or nonspecific antibody for NFB p65 isoform (p65) Resulting protein-DNA ... media overnight For inhibitor studies, chondrocytes were pretreated with the selective MEK1/2 inhibitor U0126 (10 M; Promega, Thermo Fisher Scientific, Rockford, IL, USA) [17] for 30 minutes ... were performed using the Prism 7900 HT Sequence Detector (Applied Biosystems Inc.) Samples were incubated at 48°C for 30 minutes to make cDNA templates The resulting cDNA was amplified for 40...

Ngày tải lên: 09/08/2014, 01:22

... with the informed consent of the patient or relatives Local research ethics committee approval was given for this work by the following local research ethics committees: Salford and Trafford (Project ... cellular cytospins for directly extracted and monolayer cells, and wax-embedded alginate beads sectioned at µm and mounted onto slides for analysis Immunohistochemistry was performed for aggrecan, ... Clinical diagnosis Disc level Histological grade No data L4/5 53 No data L5/S1 44 Relatively normal L4/5 47 Relatively normal L4/5 Male 75 No data L5/S1 Surgical Male ? Disc degeneration L5/S1...

Ngày tải lên: 09/08/2014, 06:22

... Articular cartilage samples with subchondral bones were fixed for days in 4% paraformaldehyde solution and then decalcified in 4% paraformaldehyde containing 0.85% sodium chloride and 10% acetic ... conditions for the indicated periods of time (24 hours) in each experiment HIF-1α R907 mRNA was quantified by RT-PCR and western blotting analysis as described above Data were analyzed for four ... evaluation and the other was stored at -30°C for later analysis by real-time PCR analysis Each cartilage sample was evaluated histologically and macroscopically for the degree of degeneration according...

Ngày tải lên: 09/08/2014, 06:23

... photographic reproductions C Meeuwisse is acknowledged for performing computer analysis of the Cyp7b promoter Dr R Hampl is acknowledged for the performance of the radioimmunoassay analysis References ... centrifuged for 10 at 8.000 N/kg, the supernatant was removed and FCS added to the residue This suspension was stirred for 30 at 45°C and the charcoal was removed by centrifugation for 10 at 8.000 ... in the same buffer for hour at ambient temperature After washing with PBS-T, the FLS were incubated for 45 with biotinylated anti-mouse IgG After washing, cells were incubated for 30 with avidin-biotin-peroxidase...

Ngày tải lên: 09/08/2014, 07:20

Ngày tải lên: 09/08/2014, 07:20

... Resuscitation and Emergency Medicine 2011, 19:28 http://www.sjtrem.com/content/19/1/28 For one, the search for new and better diagnostic biomarkers would potentially have great impact on workup ... valid nor advisable for use in the general population at large [7,8] As the authors of the current study point out [5], the high mobility group box-1 protein (HMGB1) has been known for many years ... expression It can also be released from cells, in which extracellular form it can bind to an inflammatory receptor called Receptor for Advanced Glycan Endproducts (RAGE) Activated macrophages and...

Ngày tải lên: 13/08/2014, 23:20

... idea was not successful, because for this we need to know that for fixed t (that means for fixed conjugacy class) the intersection of (3.6) with the specific conjugacy class is connected, which is not ... CHAPTER THE MAPPING CLASS GROUP 5.1 Introduction 5.2 The mapping class group for the one-holed torus 5.3 Elements of the mapping class group of the one-holed ... PSL(2, R) Conjugacy classes in PSU(1, 1) Conjugacy classes in SU(1, 1) Conjugacy classes in PSU(1, 1) and PSU(1, 1) ([DP03]) Conjugacy classes in SU(1, 1) and SU(1,...

Ngày tải lên: 13/11/2014, 09:14

... thanks to Dr Swaminathan Sethu for his critical review of the thesis, Dr Grace Low for her efforts with PCR, Mr Shriram Venkatesan and Ms Kalpana Gopalakrishnan for their help in experiments Thanks ... thanks to Mr Ganesan Arasapam for his efforts in PCRarray and Ms Cynthia and Mr Ghee Chong from the National Cancer Centre who accommodated my multiple requests for radiation time slots Sincere ... cytometry units for their many useful suggestions that greatly assisted my experiments Thank you Prof Zhao-Qi Wang and Prof Jonathan Gitlin for kindly providing the cell lines required for my study...

Ngày tải lên: 11/09/2015, 10:18

... to that Thank you for always being there for me as usual and looking out for me when I had a tough time Thank you for all the advices and for all the fun we’ve had Looking forward to sharing ... I’m looking forward to many more Thank you for being a listening ear and for helping out wherever you possibly could Thank you for sharing this journey with me Yuan Yi, thank you for also being ... you also for always listening and being supportive of our dreams and endeavors and also wanting the best for us Dr Pradeep Bist, thank you for being such an inspirational scientist, for teaching...

Ngày tải lên: 01/10/2015, 17:27

... pre incubation at 95˚ C for 10 min, the PCR was performed as follows: 35-45 cycles of denaturation at 95˚ C for sec, annealing at 60˚ C for sec, and elongation at 72˚ C for PCR product size per ... to memory formation in the brain since the hippocampus is the site of memory and the remodeling of brain is the basis for functional connections between neurons essential for memory formation ... factors for neuroinflammation in various neurodegenerative diseases Therefore, suppression of microglia-mediated inflammation has been considered as an important therapeutic strategy for neurodegenerative...

Ngày tải lên: 16/10/2015, 15:38

... It will provide an overview of the allocation of funds or the estimation of cost for the project (salaries, outsourcing costs, research fees, use of materials, hire of equipment ... • Any issues or problems that have arisen and need to be addressed Progress reports are useful for explaining the status of the project as it currently stands and its future direction They also...

Ngày tải lên: 24/04/2016, 12:10