2 preparation of the human placenta

... Marisol Sanjines (Head of Outreach) The Human Development Report Office (HDRO): The Human Development Report is the product of a collective effort Members of the National Human Development Report ... is the fruit of a collaborative effort by a team of eminent consultants and advisers and the Human Development Report team Kevin Watkins, Director of the Human Development Report Office, led the ... the consequences In the long-run, the problems of the poor will arrive at the doorstep of the wealthy, as the climate crisis gives way to despair, anger and collective security threats None of...

Ngày tải lên: 27/05/2014, 07:46

... regions of the human ANT2 promoter are shown in grey The two Sp1 AB box activation elements are shown as open symbols (B) DNA sequence of the silencer region (nucleotides )413/)235) of the human ... transfection efficiency The values of the activities are set relative to the activity of the clone carrying wt Site-2 and Site-3 elements The results represent the mean value ± S.E of five independent ... Identification of the Site-2 and Site-3 binding protein as a member of the NF1 family of transcription factors (A) Binding of purified, recombinant NF1 to the Site-2 and Site-3 elements was monitored by the...

Ngày tải lên: 07/03/2014, 15:20

... structure of human SUMO-2 is more similar to that of yeast SMT3 than to the NMR structure of SUMO-1 The difference between SUMO-2 Table Refinement procedures of the SUMO-2 crystals Description of steps ... is particularly evident in the regions of 28–43 and 71–83, that correspond to the strand b2, the N terminus of the helix a1, the helix a2, and the connecting loop to the strand b5 (Fig 3A,D) Such ... with the Gly93 of another The crystal structures of diubiquitin and tetraubiquitin showed some alternatives of the quaternary conformations of ubiquitin polymer for efficient recognition by the...

Ngày tải lên: 16/03/2014, 18:20

... and characterization of human L-RAP gene promoter Figure shows the nucleotide sequence of the 5¢-flanking region of the human L-RAP gene The transcriptional initiation site of the gene was determined ... activity These results further confirm the roles of transcription factors in the expression of the L-RAP gene Discussion L-RAP ⁄ ERAP2 is a newly identified ER aminopeptidase and the third member of the ... Differential usage of exon 15 causes the generation of two truncated forms of the transcripts These results indicate that at least three mRNAs encoding either the full length or truncated form of L-RAP...

Ngày tải lên: 23/03/2014, 13:20

... sequencing and the PCR/RFLP method As the ALI5 mutation is located in the region corresponding to the human exon 27, this exon was also screened by SSCP Results Analyses of microsatellites In the present ... of the markers did show a significant allele distribution between the patient and control group Conclusion In conclusion, our analysis of the human PLCγ-2 gene did not reveal an association of ... with a phenotype comparable to WG Therefore, human PLCγ-2 is a good candidate gene for seeking predisposing genetic factors for WG The human gene is a member of the PLC family comprising 12 closely...

Ngày tải lên: 11/08/2014, 08:20

... that the value of Ln P(D) increased consistently with increasing K However, the secondorder rate of change of the likelihood function (ΔK) showed a clear peak at K = 8, reflecting the existence of ... analysis runs For the sake of clarity, we named the seven sub-populations identified in the K = analysis based on both the geographical location and hosts of the viruses assigned with the sub-populations ... genetic differences between the 34 minor sub-populations (40.96% of the diversity) and seven major subpopulations (30.65% of the diversity; Table 1) To further test whether the 34 identified minor...

Ngày tải lên: 12/08/2014, 01:21

... and the extracted viral RNA was used for RT-PCR The Mon340 and Mon348 primers were used for the amplification of the ORF1a region, and the Mon269 and Mon270 primers for the amplification of ORF2 ... in the Neighbor program In 89 of the 120 AstV specimens isolated from 2004 to 2005, the nucleotide sequence of the partial ORF1a amplicon amplified The phylogenetic analysis results showed the ... case of ORF2, the 88 nucleotide sequences were analyzed and the phylogenetic tree was constructed The HAstV-1 prototype, the Oxford strain, and the Dresden strain clustered, unlike in the case of...

Ngày tải lên: 12/08/2014, 01:21

... Combination of the interaction data creates a consolidated set of 31,609 interactions between 7,748 human proteins On the basis of this initial set of interactions, we estimate the scale of the human ... × 10-5) shows the clustering in the human protein interaction network is considerably above that expected at random, in spite of the incompleteness of the network The 'degree' of the network is ... study the features of the network, we visualized the complete network of protein interactions in Figure On superimposing a histogram of the density of interactions on the plot, we see that there...

Ngày tải lên: 14/08/2014, 14:21

... The story of material origins is not the story the text is telling here The authors, under the guidance of the Holy Spirit, have told the part of the story that is most significant to them (the ... sider the scenario of Moses sitting down with the elders of the people on the eve of the tabernacle dedication at the foot of Sinai He is trying to help the Israelites understand the gravity of ... claim The creatures of the sea were in a liminal zone in the ancient Near East After all, the sea was the very embodiment of non-order Therefore, there would be questions about the functions of the...

Ngày tải lên: 08/06/2015, 17:26

... list of flanking sequences that were used for designing the SNP assays To ensure that the location of the SNPs map to the genome as annotated, the flanking sequences were mapped back to the human ... buffer with 1.5mM MgCl2, 1.5µl of 5µM of the forward primer, 1.5µl of 5µM of the reverse primer, 1.5µl of 100% DMSO (Sigma-Aldrich, USA), 100ng of genomic DNA and 0.2µl of High Fidelity Taq DNA polymerase ... calculating the probability of the observed data for all possible values of D' The upper (CU) and lower (CL) bounds represent the 5% tails of the overall probability For blocks that consist of or more...

Ngày tải lên: 14/09/2015, 14:07

... (Applied Biosystems) The 46 sequencing reaction mixture consisted of 4.2 μl of autoclaved water, about 300 ng of the plasmid DNA, 0.8 μl of 2.5 μM primer and μl of BigDye The PCR reaction sequencing ... times with 10 column volumes of PBS each time With the addition of 40 units of Thromin (Sigma) per ml of resin, the proteins were released from GST after four hours of incubation at room temperature; ... samples were then placed in the 50 reaction cell, and ligands were loaded into the 300 μl injection syringe Normally, the sample concentration in a syringe is 20-fold of the one in the cell Titrations...

Ngày tải lên: 14/09/2015, 14:09

... almost all of the OLP epithelium The count was performed by two investigators without knowledge of whether the samples were control or experimental The total number of basal and suprabasal epithelial ... PermountTM Negative controls consisted of omission of the primary or the secondary antibody or primary incubation in the presence of non-immunized rabbit serum instead of the primary antibody Cell quantification ... of hMSH2 Therefore, the reduced expression of hMSH2 protein could make the epithelium of OLP more susceptible to DNA mutation, making it prone to oral squamous cell carcinoma development Further...

Ngày tải lên: 03/11/2012, 09:54

... Cookies The The The The The sum of 25 and is 30 difference of 25 and is 20 product of 25 and is 125 quotient after division of 25 and is remainder after division of 25 and is As with all other programming ... changing the values of the variables to match each other, and gasp in awe as the output changes In case the equals() method doesn't appeal to you, JSP offers you a choice in the form of the compareTo() ... And if the two strings are identical, the comparison will return Incidentally, the comparison is based on both the first character of the string, and the number of characters in Flavour Of The Month...

Ngày tải lên: 19/10/2013, 02:15

... referring to parts of the human body The position of proverbs referring to part of the human body Parts of the human body 21 21 21 The classification of parts of the human body according ... diversified (multiple form) The classification of parts of the human body according to the position In the system of the proverbs, the system of words referring to parts of the human body is divided ... functions: the function of comprehension, the aesthetic function, the function of education, the words about parts of human body are used to form the meaning of proverbs, therefore, they become...

Ngày tải lên: 12/12/2013, 00:03

... earth The hand of a child - The hand of a lock The heart of a man - The heart of a matter 37 The tongue of a man - The tongue of a shoe The tooth of a person - The tooth of a comb The head of a ... hands of a farm 13 The heel of a person - The heel of the hand 14 One's leg - The leg of a trip 15 One's brain - The brains of a family 16 The bosom of a person - The bosom of the church 17 The ... part There are various types of similarity: - The similarity of shape E.g: The teeth of a saw, the head of a cabbage - The similarity of position E.g: The tail of procession - The similarity of...

Ngày tải lên: 18/12/2013, 21:45

... geothermal energy So far, even the high potential of RE resources, their contribution in electricity production in Vietnam is still negligible The Government of VN recognized important role of ... 4000 MW Geothermal energy and other types (tide, ocean waves ) Potential of geothermal energy resources: 200- 340 MW The renewable energy type such as tide and sea waves are in the stage of potential ... has been prepared by Institute of Energy Institute of Energy Main Contents of RE Master Plan and Some Preliminary results 2.1 Organization of RE MP Present status of RE development 1.1 Existing...

Ngày tải lên: 24/01/2014, 03:20

... product of PRPP from the crystallization solution ing 81 compounds of substrates, products and regulators of other enzymes in the human nucleotide metabolism (Table S1) The library can then be ... interacts with both O6 and N7 (Fig 1C) The 2¢OH of the ribose is hydrogen bonded to the main chain and the side chain of Asp142 (Fig 1C) Both hydroxyl groups of the ribose are involved in coordinating ... ligands for enzymes in the human nucleotide metabolism These were selected as substrates, intermediates and products of other enzymes in the human nucleotide metabolism The library was screened...

Ngày tải lên: 15/02/2014, 01:20

... monomer of poly(dT), showing that the changes were mainly due to the binding of the first DNA, while the binding of the second DNA had less influence (Fig 5A) To ensure that the estimation of the protein:poly(dT) ... in these eukaryotic and archaeal proteins (Fig 1A) Like the case of bacterial RecA, the L1 and L2 loops of the eukaryotic Rad51 proteins face A Roles of the HsRad51 L1 and L2 loops inside of their ... precision of 3% The changes occurring upon the addition of the element are noted in parentheses ND, not determined Y Matsuo et al ciation, like the case of Tyr188 of Xenopus laevis Rad51 [28] These...

Ngày tải lên: 19/02/2014, 06:20

... type of KLK11, isoform 2, despite the presence of exon 1c The secretory pathways of KLK11 isoforms appeared to differ, although both isoforms were secreted from cells Hence, the promoter use of the ... the isoform of translation products through the determination of the first exon in KLK11 mRNA (summarized in Fig 2A) Detection of the first exon in each KLK11 mRNA reflects the promoter use in the ... a part of isoform mRNA The third exon, exon 1c, extended from +1379 to +1454 The noncoding exons of exons 1a and 1c contributed to the 5¢ nontranslated region of isoform mRNA in which the common...

Ngày tải lên: 19/02/2014, 06:20

... functionality and phylogeny However, the nature of the connections between these factors needs to be understood at the level of the protein domain The global properties of the HTFN topology are partially ... graph of N ¼ 230 interacting human TFs was obtained (Fig 1) This can be understood as the architecture of the regulatory backbone It provides a topological view of the interaction patterns among the ... features of each group self-interaction is understood as the interaction between proteins of the same type, i.e homo-oligomerization, regardless of the number of monomers involved To evaluate their...

Ngày tải lên: 19/02/2014, 07:20