2 microfabrication techniques for engineering microvascular networks

... antifibrotic biomaterials for use in tissue engineering Unfortunately, effective characterization of antifibrotic lead compounds has been constrained by poor extracellular matrix formation in vitro, ... in a single-well format for the screening of antifibrotic compounds The Scar-in-a-Jar implements for the first time in vitro the complete biosynthetic cascade of collagen matrix formation including ... solubilization of deposited collagen for quantitation, or are too slow for use as a screening tool [6] d) The main problem faced by a biomaterial is the formation of an avascular collagen capsule...

Ngày tải lên: 10/09/2015, 15:52

... tissue formation Therefore, the properties of such biomaterials are crucial in determining the success of many tissue engineering applications Tissue engineering scaffolds act as templates for cell ... sheet engineering revolutionised the concept of Tissue Engineering when Teruko Okano et al used thermosensitive materials to create cell sheets for cardiac tissue engineering [43] Cell sheet engineering ... of Tissue Engineering  6  2.2 Biomaterials for Tissue Engineering . 7  2.2.1 Requirements and Classification of Biomaterials   7  2.3 Scaffold Fabrication Techniques: ...

Ngày tải lên: 11/09/2015, 09:10

... function of time for a single pulse exposure at r=0 for 532nm laser at a fluence of 220 J/cm2 on PCL film with a =10μm (Page 68) Fig 4.10 Temperature rise calculated for a single pulse shot for 532 nm ... showing (A) microperforations using Nd:YAG laser (at λ = 355 nm) with uniformed array of micro-size perforations on µm thick PCL film; (B) ‘donut-like’ array of micro-size perforations on 10 µm ... and medical imaging) required for tissue engineering [15] 13 Tissue engineering of bone is one recent breakthrough that has been made in the development of a platform technology which integrates...

Ngày tải lên: 14/09/2015, 08:36

... reported for as-polymerized PLLA (7), for PLLA (8), for PLLA-fiber-reinforced PLDLA 70/30 (9), for PDLLA (10), and for PLA50 samples (11) Li et al have proposed a degradation model for PLA50 ... (29) For each application the total porosity should be high for example, for cartilage tissue engineering it should be 92–96% (30) Several criteria define the ideal material for tissue -engineering ... hyaluronan-gelatin composite sponge Tissue Engineering 5, 545–554 45 Doser, M (1999) Criteria for the selection of biomaterials for tissue engineering, in Polymers for Medical Technologies, 37th Tutzing-Symposion...

Ngày tải lên: 10/04/2014, 11:11

... formulation can provide for orally dosed DCU is not well understood [14] In order to understand the degree of improvement provided by an oral nanosuspension formulation, simulations were performed ... suspension [14] as the base case and predictions were performed for higher doses (10 and 30 mg/kg) with nanosuspension formation Results for 3, 10, and 30 mg/kg are listed in Table According to ... overcome these limitations, a suitable formulation was needed to maximize the potential of DCU in vivo Several formulations strategies for SC dose have been assessed Formulations such as emulsions and...

Ngày tải lên: 21/06/2014, 03:20

... [15] B Conventional techniques for inducing pulpal repair Calcium hydroxide has long been the "gold standard" for pulp capping [16] Its effectiveness at promoting dentinal bridge formation over small ... pathogens These techniques, which include nonsurgical techniques such as scaling and root planing and surgical procedures such as open flap debridement for access and resective techniques, are ... apical foramen These two latter models have an inherent self-reparative capacity and share more similarity to human deciduous teeth and permanent teeth with immature root formation Therefore,...

Ngày tải lên: 11/08/2014, 23:22

... regarded as most promising in skeletal tissue engineering by some researchers A recently elaborated approach in extracorporal tissue engineering is therefore the avoidance of non-degradable scaffolds, ... of tissue engineering are better revealed Additionally, as the newly formed tissue is avoid of an artificial material, it more closely resembles the in vivo situation Cell sources for micromass ... the formation of species-related cartilage-like or bone-like tissue However, conditions allowing cartilage formation in one species are not necessarily transposable to other species Therefore,...

Ngày tải lên: 11/08/2014, 23:22

... tissue 1.1.3 Current vascularization strategies for engineered tissues There is therefore a clear need for effective vascularization strategies for engineered tissues, and many different approaches ... stimulus for angiogenesis to occur and progress Conversely, newly formed blood vessels can regress if there are more anti-angiogenic signals than pro-angiogenic signals at a given point Therefore, for ... drug, a better approach for the delivery of PHIs is necessary We therefore propose the use of PHI-delivering scaffolds as a strategy for improving vascularization in tissue engineering applications...

Ngày tải lên: 09/09/2015, 11:11

... communication with a PC can be performed, either for remote operations via a terminal program or for downloading a compiled BASIC-program to the microcontroller for autonomous control reactions ... navigation Robots for sewage pipe inspection and repair Electrical wheelchairs for disabled people The Mobile Instrument Deployment Device (MIDD), developed for Mars exploration in contract for the European ... handling equipment, etc, which allow for very flexible implementations Basic Level Components For introductory courses, a low-cost control system is used This allows for interesting and engaging experiments...

Ngày tải lên: 05/03/2014, 15:20

... control The scoring algorithm for the scales is similar to the scoring of the EORTC-C30 questionnaire RS are computed and linearly transformed to a - 100 scale For the scales and items, a higher ... QoL were also compared for the time points before and months after the start of VMAT Fourteen (77.8%) patients completed questionnaires at both time points The reason for not completing the questionnaires ... Self-assessed QoL (EORTC-C30) before and during VMAT for Global Health Status, and physical, emotional and role functioning 3-months follow-up, patients had a significantly worse performance status Mean...

Ngày tải lên: 09/08/2014, 09:20

... Leblond AL, Naud P, Forest V, Gourden C, Sagan C, Romefort B, Mathieu E, Delorme B, Collin C, Pages JC, Sensebe L, Pitard B, Lemarchand P: Developing cell therapy techniques for respiratory disease: ... efficient reprogramming, avoiding the use of oncogenes for inducing pruripotency, and maximizing yield of new promising cell sources for tissue engineering Epithelial cell-extracellular matrix interactions ... substitutes could not Most cartilage tissue engineering approaches use biodegradable three-dimensional (3D) scaffolds, which are important for neocartilage formation by promoting chondrocyte attachment,...

Ngày tải lên: 10/08/2014, 05:21

... important determinant for HIV-1 cell tropism Results Vpu S52A impairs tetherin and CD4 degradation in transfected 293T cells For functional analyses, we generated untagged and AU1-tagged forms of the ... infection for CD4 and tetherin expression as already described [34] Similarly, primary macrophage cultures were trypsinized five days post infection and stained for CD4 and MHC-I expression as before ... PHA for 24 hours (PBMC+) Click here for file [ http://www.biomedcentral.com/content/supplementary/1742-4690-7-1S2.PDF ] Additional file 3: Supplementary Figure S3 Vpu S52A is dispensable for...

Ngày tải lên: 12/08/2014, 23:22

Ngày tải lên: 21/08/2014, 10:51

... Haematoma formation linking bone fragments • Recruitment of fibroblasts, MSC • Granulation tissue formation Soft callus formation • • • • Hard callus formation • • • Bone remodelling • • • Cartilage formation ... Yokohama, Japan 2012 - 1st Prize, “Speak Out For Engineering" Local Heats by Institution of Mechanical Engineers 2012 - 2nd Prize, “Speak Out For Engineering" Regional Heats (Oceania and Asia) ... Cues .26 2.6 Bone Tissue Engineering 30 2.6.1 Four-Stage Bone Formation in Bone Tissue Engineering 30 2.6.2 Strategies in Bone Tissue Engineering .32 xi 2.6.2.1 Growth...

Ngày tải lên: 09/09/2015, 10:16

... anti-fibrotic properties, PHi may be a potent aid for tissue engineering, by tackling both the problem of fibrosis and vascularization requirements Therefore, the aim of this research study was to ... Willebrand Factor: Markers for Endothelial Cells iii 3.1.2 40 3.1.3 PHi Induced Angiogenesis in Sequential Co-cultures 49 3.1.4 3.2 Development of Sequential Co-culture for Proangiogenesis Quantification ... 91 4.3 Quantification of Sequential Co-culture Angiogenesis 92 4.4 Fibroblasts Are Required For HUVEC Formation of Capillary Like Structures 93 4.5 PHi Induced HIF-1α and VEGF 95 4.6 Zebrafish...

Ngày tải lên: 12/09/2015, 08:18

... EC networks These networks were characterized by long extensions of EC CLS with perceptible junctions (white arrows) The lack of the GFs in the media (-ve control) prevented the networks from forming ... scaffold would have a cellpenetrable fibrous zone for the formation of fibrous tissue and vascular networks Beneath this zone, a cambium layer is situated for the possible delivery of mesenchymal cells ... material engineering, tissue engineering is expected to make critical impacts on the patient’s quality of life 9 Scaffold-based tissue engineering The concept of scaffold-based tissue engineering...

Ngày tải lên: 14/09/2015, 08:24

... tissue system to be studied 1.2 Vascular Tissue Engineering Vascular tissue engineering presents an attractive model for LBL tissue engineering for several reasons Firstly, vascular tissue is ... (UCB-EPC) were deemed the most suitable for vascular tissue engineering due to lineage stability The feasibility of microthin PCL films for layered vascular tissue engineering was explored µXPCL film ... Cell sheet engineering 1.1.3 Layer-By-Layer Tissue Engineering Using Cell-Scaffold Hybrid Constructs 11 1.1.3.1 1.2 Microthin films for use in layer-by-layer tissue engineering...

Ngày tải lên: 14/09/2015, 08:49

... like to thank her for reviewing the drafts  Dr Simon Cool (IMB), project mentor, for his suggestions given on the progress of this project during presentations  Richard Lin (3M), for his assistance ... Instron micro tester for mechanical testing  Ms Irene Kee (Dept of Experimental Surgery, SGH), for her valuable assistance and support during animal surgeries and taking care for them after implantations ... current treatments for bone defects For surgical procedures involving bone grafts, patients usually suffer from trauma-related injuries or bone fractures Currently, the gold standard for bone grafts...

Ngày tải lên: 09/10/2015, 11:18

... coined as “bone tissue engineering A key component in tissue engineering for bone regeneration is the scaffold that serves as a 3D template for initial cell interactions and the formation of boneextracellular ... (stereolithography) format into QS (QuickSlice), slicing of the CAD model into horizontal layers and conversion into SLC format, creation of a deposition path for each layer and conversion into SML format for ... source of inspiration and motivation for this project throughout  Dr Alvin Yeo (Department of Mechanical Engineering and National Dental Centre), co-supervisor, for his patience and guidance on...

Ngày tải lên: 09/10/2015, 11:18