2 in vitro engineering of retinal cell types

... 24 rof derutam setycoo TNCS fo ecnetepmoc latnempoleved desaerced taht detseggus ]31[ reklöH ]61[ h 44 rof derutam esoht naht TNCS retfa noitamrof tsycotsalb dna egavaelc rehgih dewohs h 33 rof ... tnempoleved oyrbme rof muidem )CVI( erutluc ehT stnemirepxe lla ni desu saw hctab emas eht morf diulf ralucillof enicroP esu litnu Co02− ta derots dna ,deretlif ,nim 51 rof × 009,1 ta degufirtnec ... derots dna ,deretlif ,nim 51 rof × 009,1 ta degufirtnec ,retemaid ni mm 8-3 selcillof morf detcelloc saw diulf ralucillof enicroP dna stnemirepxE ni muidem denifed a sa desu dna )AVP-MCT( lohocla lynivylop...

Ngày tải lên: 07/08/2014, 20:23

... DEVELOPMENT OF A BIOREACTOR FOR IN -VITRO ENGINEERING OF SOFT TISSUES KYAW MOE (B.Eng (Hons.), YTU, Yangon) A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF ENGINEERING DEPARTMENT OF MECHANICAL ENGINEERING ... tissue -engineering approach to tissue repair and regeneration is the availability of appropriate cells The presence of cells is crucial; this is because of their proliferation potential, cell- to -cell ... water and cells [Akeson et al, 1984] Water makes up about 55% of wet weight of tendons and 60-80 % of wet weight of ligaments Collagen is arranged in the form of fibers within a matrix of GAGs,...

Ngày tải lên: 04/10/2015, 15:46

... the reduction in cell number may have been the result of a general and simultaneous blockade of all three phases of the cell cycle Cell proliferation is reduced The reduction in cell number may ... may have been a result of an inhibition of cellular proliferation Therefore, at the end of each of the culture schedules, cells were pulsed with BrdU for 30 mins The extent of BrdU incorporation ... investigated in GEO cells only, as inhibition of cell proliferation was not seen in the HCT116 cells At the end of the treatment schedules, the surviving fractions of GEO cells were plated in...

Ngày tải lên: 07/03/2014, 15:20

... Intensity of cytoplasm Ratio of Translocation = Intensity of nucleus Intensity of cytoplasm Intensity of nucleus Scheme This is a simplication of a cell as seen by analysis software, where the software ... not just in vitro and cell based but also simulated in silico Combining this method of analysis (in vitro, cell- based and in silico analysis) will facilitate systematic understanding of the underlying ... in single cell studies In our cell- based experiments, a population of 10 000 A549 cells was observed to undergo similar oscillatory behavior to that discovered in single cells in terms of the peak...

Ngày tải lên: 16/03/2014, 11:20

... of octacalcium phosphate [56] in addition to other types of complexes Based on the interpretation of its IR spectrum, the mineral deposit obtained after hydrolysis of ATP contains a mixture of ... inhibitors of GPI-bALP, different types of mineral deposits such as OCP and HPO42– complexes of calcium were obtained The lack of HA formation is probably associated with the presence of the pyrophosphate ... supplemented in bone cells, chondrocytes and cultured cell types to initiate mineralization and to better monitor different aspects of mineral formation [25,31–40,58,64–68] The mechanism of mineralization...

Ngày tải lên: 23/03/2014, 17:21

... http://www.translational-medicine.com/content/7/1/2 Figure Characterization of T cell responses Characterization of T cell responses (A) CD8+CD107a+ effector cells, (B) CD8+perforin+ effector cells, (C) PD-1+ T cells, (D) CD4+CD25+FoxP3+ Tregs ... titer ≥ fold of the baseline titer Page of 11 (page number not for citation purposes) Journal of Translational Medicine 2009, 7:2 T cell responses The IFN-γ ELISPOT was used to monitor T cell responses, ... from the cell membrane and thus can mediate antibody-dependent cell- mediated cytotoxicity (ADCC) In addition, 5T4-transduced renal carcinoma cell lines can be recognized by human T cells in vitro, ...

Ngày tải lên: 18/06/2014, 15:20

... maturation of oocytes during in vitro maturation of mouse COCs [9], the EGF-like factor was quite important for ovulatory process During in vitro maturation of porcine COCs, the addition of EGF to ... cumulus cells cultured with FSH and/or LH have remained unclear during in vitro maturation of porcine COCs http://www.ovarianresearch.com/content/2/1/20 The binding of FSH and/or LH in granulosa cells ... function of cumulus cells during meiotic resumption of porcine oocytes [23] RNA isolation After cumulus cells were cultured, they were washed three times in PBS Total RNA was extracted from cumulus cells...

Ngày tải lên: 20/06/2014, 07:20

... chordoma cells at National Institute of Radiological Sciences (NIRS), has acceptably shorter cell doubling time that enabled us to carry out in vitro cell biological research such as clonogenic cell ... content (about 60) of U87-MG Cell cycle distribution in chordoma cells showed a significantly high ratio in G1phase, very different from the DNA profile patterns of the other two cell lines These ... http://www.ro-journal.com/content/6/1/116 Page of Figure Characters of cell lines A) Growth curves for three cell lines Black reverse triangle indicates HeLa cells, white circle indicates U87-MG glioma cells, and black circle...

Ngày tải lên: 09/08/2014, 09:21

... flasks At least two different cell line have been used Accumulation of cAMP was measured in cells grown to confluence in 12-well plates and serum-starved for 24 h Cells were incubated at 37°C ... rings were passively sensitized by using a pool of sera containing high levels of specific IgEs but low levels of total IgEs With this method of passive sensitization and allergen challenge, ... 50% of active force in Salbutamol concentrations inhibiting 50% of active force in carbachol-contracted human bronchial rings Effects of montelukast on salbutamol concentrations inhibiting 50% of...

Ngày tải lên: 12/08/2014, 16:20

... the expression of IL-6 and IL-8 Because of that, we studied the expression of mPGES and measured the production of PGE2 and we showed in vitro that mPGES expression as Page 10 of 12 well as PGE ... panels: Western blot of conditioned media with IL-6 and IL-8 antibodies; CD) Quantitation of the repression of IL-6 and IL-8 by MGDG The average of densitometric analysis of Western blots performed ... measure the activation of p38 taking advantage of a commercially available ELISA assay that allows determination of phospho-p38, total p38 and cell number in the same assay Cells were treated with...

Ngày tải lên: 12/08/2014, 17:21

... rhabdoid tumor cells lines treated with 4-HPR and its derivatives To determine the effect of 4-HPR derivatives on the cell cycle profiles of RT cells we carried out FACS analysis of cells treated ... changes in the cell cycle profiles of these cells In MON cells, 4-HPR slightly increased the percentage of cells in S-phase, while both iododerivatives induced significant S-phase cell cycle arrest ... Though the nature of induction of cell- cycle arrest by 4-HPR and its derivatives varied from cell type to cell type, fluorescence activated cell sorting (FACS) analysis indicated that cell death, as...

Ngày tải lên: 13/08/2014, 18:21

... died off Explant died off Explant died off Explant died off Explant died off Explant died off Explant died off Explant died off Explant died off Explant died off Explant died off Explant died off ... died off Explant died off Explant died off Explant died off Explant died off Explant died off Explant died off Explant died off Explant died off Explant died off Explant died off Explant died off ... emerging from the distal end of lTCL, few in number Soft, brownish yellow calli emerging from the distal end of lTCL Explant died off Explant died off No callogenesis Friable, soft, milk- and transparent-white...

Ngày tải lên: 20/04/2015, 21:33

... template, or a combination of such factors (Chapter 4) In such Analysis, sequencing and in vitro expression of PCR products 89 cases it is often possible to increase the sensitivity of the analysis in ... original primers Of course in this case the use of an enzyme with 3′-exonuclease proofreading activity should be avoided to protect the differentiating 3′-end An example of the design of original ... product identity Some combination of approaches may be required in difficult cases Of course the most definitive confirmation of identity of a PCR product is determination of its DNA sequence, a process...

Ngày tải lên: 25/10/2013, 22:20

... exposure of HepG2 cells potentiates proliferation by a PI 3-kinasemeditated activation of the transcription factor activator protein AP-1 [22], corroborating a critical role of cell swelling for cell ... the cell cycle machinery and how cell shrinkage sensitizes cells to apoptotic stimuli requires further scientific effort Cell hydration may markedly affect the action of drugs For example cell ... execution of the apoptotic program Concluding remarks It is well acknowledged that cell volume fluctuations release signals of (patho)physiological relevance The understanding of how cell swelling...

Ngày tải lên: 18/02/2014, 16:20

... Effect of DDI-1A and DDI-1B on repeats expansion DDI-1A and DDI-1B were tested for their effect on the expansion of several doublet and triplet repeats in the presence of the Klenow fragment of DNA ... markers of 26 and 41 nucleotides (random sequence) in length *The 5¢-32P-end-labeled strand (Inset) Special attention of triple band pattern in the gel of DDI-1A was better than that of DDI-1B, ... control was remarkable Effect of different polymerases on drugstimulated replication of the ATTÆAAT triplet As shown in Fig 3, we also investigated the effect of a series of different prokaryotic...

Ngày tải lên: 07/03/2014, 06:20

... of listing of species Germplasm collection of prioritised species and collection of samples for testing of nutrient value, impact on rumen function and anthelmintic effects Field evaluation of ... improve the efficiency of nutrient utilization Usually, the initial screening is conducted in vitro because of the large number of candidate plant species and the prohibitive cost of screening them ... groups use botanical information that is available about families of plants and the likelihood of the presence of particular types of secondary compounds as a starting point, whereas others use...

Ngày tải lên: 15/03/2014, 04:20

... ratio of DNA/protein of 1.09 : and an average ratio of RNA/protein of 1.12 : Multiple-turnover (substrate in excess) DNA cleavage reactions were also performed to estimate the stoichiometry of DNA ... believed to result from slow release of the cleavage products from the protein The amplitudes of the initial burst (2.78 and 2.83 nM) gave a ratio of DNA/protein of 0.94 : As will be discussed further, ... concentration of KCl, there was a correlation between inhibition of endonuclease activity and inhibition of splicing activity indicating that RNA binding can be monitored by measuring inhibition of the...

Ngày tải lên: 17/03/2014, 10:20

... strain; (C) Ratio of O2 consumption in (A) over (B) Ó FEBS 2003 Fig 1B) However, O2 consumption was stimulated strongly in both membrane types (Fig 1A,B) in the presence of 0.2 mM of ubiquinone ... addition of NaOH to a 0.2 M final concentration) The concentration of NaCl was also increased in a separate assay PTOX activity is given as percentage of starting O2 consumption (before addition of ... concentration of the given inhibitors Activity before addition of inhibitor is set at 100 (absolute amount were calculated as explained in Table 1) Inhibitors of PTOX activity in vitro It is worth...

Ngày tải lên: 31/03/2014, 07:20

... Effect of molarity and pH on the diffusion profile of ketoprofen .85 Effect of temperature on the diffusion profile of ketoprofen 86 Effect of mass on the diffusion profile of ketoprofen ... the analysis of ketoprofen 58 The effect of mobile phase composition on the retention time of ketoprofen 62 Effect of wavelength on the relative percent peak area of ketoprofen 63 ... diffusion cell and European Pharmacopoeia diffusion cell comparison of the in vitro release of ketoprofen from proprietary formulations (n = 5) .137 Figure 5.25 Effect of different grades of Carbopol®...

Ngày tải lên: 13/04/2014, 09:25

... of fusion cells and have a negative impact in the stimulation of T cells To determine the induction of WT1-specitic CD8+ T cells, a pentameric assay of soluble class I MHC-peptide com- Page of ... fusion cells created in the presence of HCCsp Phenotypic analysis of DCs/allo-HCC fusion cells created in the presence of HCCsp A, Four types of DC were analyzed by flow cytometry for expression of ... stimulation with the types of fusion cell preparation from four healthy donors, percentage of IFN-γ-positive CD4+ or CD8+ T cells was assessed E, After stimulation with the types of fusion cell preparation...

Ngày tải lên: 18/06/2014, 15:20