2 human induced pluripotent stem cell derivation
báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
Ngày tải lên :
11/08/2014, 12:21
... Belmonte JC: Methods for making induced pluripotent stem cells: reprogramming a la carte Nat Rev Genet 2011, 12:231-242 Takahashi K, Yamanaka S: Induction of pluripotent stem cells from mouse ... epigenomes (left panels) The extent of reprogramming and differentiation potential of individual induced pluripotent stem cell (iPSC) lines can be assessed by comparing iPSC epigenomes (right panels) ... variants, disease-relevant cell types can be derived from healthy and disease-affected donor induced pluripotent stem cells (iPSCs) and epigenomic profiling can be used to evaluate how these genetic...
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Human pluripotent stem cell derived cellular vehicles for cancer gene therapy
Ngày tải lên :
09/09/2015, 10:08
... cells is feasible, and that contaminated cells are completely removed 1.2.1.1.2 Induced pluripotent stem cells Induced pluripotent stem cells (iPSCs) were first generated in the Takahashi and Yamanaka ... scale production of therapeutic stem cells must be identified Pluripotent stem cells including embryonic stem cells and induced pluripotent stem cells may hold the answer to this question VIII ... proposes pluripotent stem cells as potential alternatives to derive therapeutic cells for this purpose IX LIST OF PUBLICATIONS 1.Tumor tropism of intravenously injected human -induced pluripotent...
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báo cáo khoa học: " Capturing Alzheimer’s disease genomes with induced pluripotent stem cells: prospects and challenges" pps
Ngày tải lên :
11/08/2014, 12:21
... monogenic and complex diseases with pluripotent stem cells Nat Rev Genet 2011, 12:266-275 33 Gonzalez F, Boue S, Belmonte JCI: Methods for making induced pluripotent stem cells: reprogramming ... fAD, familial AD; GWAS, genome-wide association study; hESC, human embryonic stem cell; iPSC, induced pluripotent stem cell; NPC, neural precursor cell; NSC, neural stem cell; PD, Parkinson’s disease; ... Lowry WE, Benvenisty N: Identification and classification of chromosomal aberrations in human induced pluripotent stem cells Cell Stem Cell 2010, 7:521-531 36 Laurent LC, Ulitsky I, Slavin I, Tran...
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Báo cáo y học: " Induced pluripotent stem cells free of exogenous reprogramming factors" pot
Ngày tải lên :
14/08/2014, 21:20
... JA: Induced pluripotent stem cell lines derived from human somatic cells Science 2007, 318:1917-1920 Okita K, Nakagawa M, Hyenjong H, Ichisaka T, Yamanaka S: Generation of mouse induced pluripotent ... approach for the generation of induced pluripotent stem cells Cell Stem Cell 2008, 2:525-528 Shi Y, Desponts C, Do JT, Hahm HS, Schöler HR, Ding S: Induction of pluripotent stem cells from mouse ... vectors Science 2008, 322:949-953 Stadtfeld M, Nagaya M, Utikal J, Weir G, Hochedlinger K: Induced pluripotent stem cells generated without viral integration Science 2008, 322:945-949 Soldner...
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ENGINEERING THREE DIMENSIONAL CULTURE PLATFORMS FOR HUMAN ADIPOSE DERIVED STEM CELL THERAPY
Ngày tải lên :
09/09/2015, 11:18
... microspheres HGF Hepatocyte growth factor HUVEC Human umbilical vein endothelial cells iPSCs induced pluripotent stem cells MSCs Mesenchymal stem cells OCN Osteocalcin xxiv PBS Phosphate buffered ... few decades away, few significant milestones have already been reached, such as generation of induced pluripotent stem cells (iPSCs) (Takahashi et al 2006), isolation of stem cells from adipose ... the stemness properties of ADSC-GMs by conducting gene expression studies for the four well known pluripotent markers genes Oct4, Sox2, Nanog and Rex1 We found that all these genes were significantly...
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Screen of nuclear receptors for the enhanced and alternative generation of induced pluripotent stem cells
Ngày tải lên :
09/09/2015, 18:56
... undone The resultant cells, known colloquially as induced pluripotent stem cells (iPSCs), become akin to pluripotent embryonic stem cells (ESCs) and pluripotent cells of the early embryo, thus gaining ... cells can also be induced to fuse with one another in vitro For instance, when a somatic cell fuses with a pluripotent cell such as an ESC, the resultant hybrid cell still remains pluripotent and ... embryonic germ (EG) cells that are pluripotent 42 This technique is common in germ cells because they possess pluripotential characteristics In another instance, pluripotent cells can also be derived...
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Generation of porcine induced pluripotent stem cells and their differentiation into cardiac lineages
Ngày tải lên :
30/09/2015, 10:13
... transitioning from fibroblasts to Induced Pluripotent Stem Cells 45 iv 4.10 Culture of Porcine Induced Pluripotent Stem Cells 45 4.10 Characterization of Porcine Induced Pluripotent Stem Cells ... cells have been isolated, induced pluripotent stem cells for the pig must be used as a source of pluripotent cells Hence, fibroblasts from the pig is taken, and induced into pluripotent cells These ... 4.7 Packaging of Lentiviruses for the induction of Pluripotent Stem Cells………………… 44 4.8 Infection of fibroblast cells to form Induced Pluripotent Stem Cells ……………………………………………………………………...
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Human embryonic stem cell derived neural stem cells derivation, differentiation and MicroRNA regulation
Ngày tải lên :
10/09/2015, 09:08
... Interferon Interleukin Induced pluripotent stem Luria broth Leukemia inhibitory factor Mammalian achaete-scute homologue Multiple cloning site Morpholineethanesulfonic acid MicroRNA -induced silencing ... overexpression of PAX6 represses pluripotent genes and promotes neural induction They further demonstrated that while both PAX6a and PAX6b induced the repression of pluripotent genes only PAX6a promoted ... mechanisms of regulation of NSC differentiation 1.1.5.2 Pluripotent stem cell-derived NSCs Human embryonic stem cells (hESCs), which are pluripotent cells derived from the inner cell mass of blastocyst-stage...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 2
Ngày tải lên :
11/09/2015, 10:06
... IPI00655812 IPI00021812 metalloproteinase with thrombospondin motifs 1*† Transforming growth factor-beta -induced protein ig-h3 74665 122841 234698 Isoform of Extended synaptotagmin-1*† Isoform of Myoferlin*†...
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Guidelines for Human Embryonic Stem Cell Research potx
Ngày tải lên :
06/03/2014, 15:20
... rise to permanent pluripotent stem cell lines in culture, mEG cells (Matsui et al., 1992; Resnick et al., 1992) Under appropriate culture conditions, hES cells were shown to be pluripotent by differentiating ... be derived and their genotype will be identical with that of the nuclear donor Successful derivation of pluripotent mES cells from cloned NT blastocysts has been demonstrated in mice by several ... directly—not by transfer into oocytes but by other means, such as fusion with pluripotent ES cells or exposure to factors from such pluripotent cells INTERSPECIES MIXING Interspecies mixing happens in...
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Báo cáo Y học: GPI-microdomains (membrane rafts) and signaling of the multi-chain interleukin-2 receptor in human lymphoma/leukemia T cell lines doc
Ngày tải lên :
17/03/2014, 23:20
... taken to keep the cells at ice cold temperature before FRET measurements in order to avoid unwanted induced aggregations of cell surface molecules or significant receptor internalization Labeled cells ... membrane fraction was collected from the 5–36% sucrose interface where it formed a visible band IL-2 induced tyrosine phosphorylation of STAT3 (and STAT5) was followed by flow cytometry as described ... preassembly of the three IL-2R chains in the plasma membrane of T leukemia/lymphoma cells is not induced by ligand binding, as in case of other growth factor receptors (e.g EGFR) [8] Furthermore,...
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Atlas of Human Pluripotent Stem Cells doc
Ngày tải lên :
22/03/2014, 09:20
... reprogramming of adult skin fibroblasts into induced pluripotent stem cells (Takahashi and Yamanaka, 2006) To realize the full potential of embryonic and induced pluripotent stem cells, technologies, ... Human Embryonic and Induced Pluripotent Stem Cell Colonies Cultured with Feeders Abstract To prolong the stage of undifferentiation, human embryonic stem cells (hESCs) and induced pluripotent stem ... pleased to present this Atlas on Human Pluripotent Stem Cells— Derivation and Culturing, summarizing 12 years of our team’s experience, skill and knowledge in the derivation, culture and expansion...
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Chapter 066. Stem Cell Biology (Part 2) pdf
Ngày tải lên :
07/07/2014, 01:20
... can form an entire organism autonomously Only a fertilized egg (zygote) possesses this feature Pluripotent cells (e.g., ES cells) can form almost all the body's cell lineages (endoderm, mesoderm,...
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Báo cáo y học: "A mouse embryonic stem cell bank for inducible overexpression of human chromosome 21 genes." ppsx
Ngày tải lên :
09/08/2014, 20:22
... to detect differentially expressed genes (that is, in induced versus non -induced cells) We first normalized together both induced and non -induced hybridizations, and then detected differentially ... there was a significant difference in expression between the two groups of microarrays (induced versus uninduced) P-value adjustment for multiple comparisons was done with the FDR of BenjaminiHochberg ... http://genomebiology.com/2010/11/6/R64 pressing clone: replicates for the non -induced state and replicates for the induced state (48 hours) All of the above samples were always run simultaneously...
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Báo cáo y học: "A comparative analysis of DNA methylation across human embryonic stem cell lines" docx
Ngày tải lên :
09/08/2014, 23:20
... differentiation of the ESCs, and is restored in induced pluripotent stem cells [15], suggesting there may be a functional role for non-CG methylation in pluripotent stem cells However, less is known ... methylation profiles between ESCs (or multipotent progenitors) and differentiated cells [15-18] and induced pluripotent stem cells [19,20] These vertical comparisons provide valuable insights into the ... Ren B, Thomson JA, Evans RM, Ecker JR: Hotspots of aberrant epigenomic reprogramming in human induced pluripotent stem cells Nature 2011, 471:68-73 Cokus SJ, Feng S, Zhang X, Chen Z, Merriman B,...
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Báo cáo y học: " Human embryonic stem cell (hES) derived dendritic cells are functionally normal and are susceptible to HIV-1 infection" pot
Ngày tải lên :
10/08/2014, 05:20
... single positive for CD1a and DC-SIGN which could represent other DC subtypes Figure Derivation of DCs from hES cells Derivation of DCs from hES cells: Undifferentiated hES cells were cocultured with ... capable of supporting HIV-1 infection Results Hematopoietic differentiation of human ES cells and derivation of dendritic cells hES cell line H1 was propagated as undifferentiated cells by co-culture ... way for utilization of these cells for hematopoietic cell therapy [9] Recently we demonstrated derivation of phenotypically and functionally normal macrophages from hES-CD34+ cells and established...
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Overexpression of hTERT increases stem-like properties and decreases spontaneous differentiation in human mesenchymal stem cell lines ppt
Ngày tải lên :
10/08/2014, 05:21
... twice per week For osteogenic differentiation, cells were seeded at a density of 104 cells/cm2 and induced in DMEM-LG supplemented with 10% FBS, 50 μg/ml ascorbate-2 phosphate (Nacalai, Kyoto, Japan, ... such as MSCs, and should serve as an indicator to discriminate ESCs from MSCs [29-31] We therefore induced 3A6 to undergo osteogenic and neural differentiation and examined the expression of Oct4 ... far beyond the normal hMSCs They expressed trophoectoderm and germline specific markers at day of induced differentiation with BMP4 and RA, respectively Besides unlimited differentiation potential,...
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