2 hapten presentation by langerhans cells lc
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Báo cáo Y học: Complexation of ytterbium to human transferrin and its uptake by K562 cells pot
... which was obtained by integrating the intensity per cell from · 104 cells for each sample Laser scanning confocal microscopy Entry of FITC-Yb-Tf into K562 cells was investigated by LCSM A suspension ... K562 cells The binding of Yb-bound Tf species in solution with 2.5 : mol ratio to K562 cellular membrane was quantitatively evaluated by sorting the cells after incubation with FITC-Yb2-Tf by flow ... transport of FITC-Yb3+-apo-Tf into K562 cells observed by LCSM LSCM was employed to visualize the entry of FITC labeled Yb-bound Tf species into K562 cells In Fig 8A,B, the green fluorescence...
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JavaFX 2.0: Introduction by Example pptx
... calculation Setting the horizontal and vertical gaps relate to the spacing between UI controls within the cells Next is simply putting each UI control into its respective cell location All cells ... the radius We begin by implementing the computeValue() method to perform our calculation of the volume Shown is the low-level binding scenario to compute the volume of a sphere by overriding the ... The title of the book says it all: JavaFX 2.0 Introduction by Example In this book, you will be learning the new JavaFX 2.0 capabilities by following practical recipe examples These recipes will,...
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Báo cáo khoa học: Probing the catalytic potential of the hamster arylamine N-acetyltransferase 2 catalytic triad by site-directed mutagenesis of the proximal conserved residue, Tyr190 pot
... intermediate (k2) followed by rate-limiting hydrolysis (khydrolysis) [26] (Scheme 1) Each of the Y190 mutants exhibited similar burst kinetics, followed by rate-limiting deacetylation by water (Table 1) ... representation of the model structure of hamster NAT2 The catalytic triad is colored in red and Y190 is in green (B) Expanded view of selected residues of hamster NAT2 Residues are colored by ... Y190 mutants are correctly folded, as shown by CD, the specic activity was determined as the transacetylation reaction rate of PNPA PABA catalyzed by wild-type and Y190 mutants with saturating...
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HOW TO BRIDGE THE DISTANCE BETWEEN BUSINESS STRATEGY AND DESIGN.A VISUAL PRESENTATION BY MARTY potx
... A VISUAL PRESENTATION BY MARTY NEUMEIER Produced by NEUTRON LLC in partnership with NEW RIDERS PUBLISHING and THE AMERICAN INSTITUTE OF GRAPHIC ARTS Copyright © 2003 Neutron LLC For educational ... INSTITUTE OF GRAPHIC ARTS Copyright © 2003 Neutron LLC For educational use only No part of this presentation may be published, sold, or otherwise used for profit without the written permission ... YOU’LL LEARN: A modern definition of brand The five disciplines of brand-building READY? LET’S START BY DISPELLING SOME MYTHS FIRST A brand is not a logo SECOND A brand is not an identity X FINALLY...
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The Eugenic Marriage, Vol 2 (of 4), by W. Grant Hague This eBook is for the use of anyone anywhere at no cost and with almost no restrictions whatsoever. You may copy it, give it away or re-use it under the terms of the Pro pptx
... —FOOD FORMULAS Baby's bed—The proper way to lay baby in bed—Baby should sleep by itself—How long should a baby sleep— Why a baby cries—The habitual crier— The habit of feeding baby every time it ... important—A baby never vomits—What is the significance of socalled vomiting after feedings— Mother's milk that is unfit for baby— Fresh air for baby—Air baths for baby Page 223 CHAPTER XVIII BABY'S GOOD ... of walking the floor with baby every time it cries—Jouncing, or hobbling baby—Baby needs water to drink—The evil habit of kissing baby— Establishing toilet habits—Baby's comforter—What can be...
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JavaFX 2.0: Introduction by Exampleprovides pot
... calculation Setting the horizontal and vertical gaps relate to the spacing between UI controls within the cells Next is simply putting each UI control into its respective cell location All cells ... the radius We begin by implementing the computeValue() method to perform our calculation of the volume Shown is the low-level binding scenario to compute the volume of a sphere by overriding the ... The title of the book says it all: JavaFX 2.0 Introduction by Example In this book, you will be learning the new JavaFX 2.0 capabilities by following practical recipe examples These recipes will,...
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Báo cáo khoa học: Transcription of mammalian cytochrome c oxidase subunit IV-2 is controlled by a novel conserved oxygen responsive element pptx
... stimulated by hypoxia and mediated by a novel oxygen responsive element (ORE) Wildtype and site-directed mutagenesis constructs of the human CcO42 promoter were transfected into human H460 cells that ... ROS generation by increasing the ubisemiquinone pool [10] As discussed above, regulation of electron flux at CcO by subunit 4-2, in analogy with ATP regulation of CcO activity by subunit 4-1 [23], ... supplier’s protocol Transfection and luciferase assay H460 cells were plated onto 24-well plates at · 104 cells ⁄ well and grown overnight Cells were transfected using TransFast (Promega) with lg...
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báo cáo hóa học: " Astrocyte production of the chemokine macrophage inflammatory protein-2 is inhibited by the spice principle curcumin at the level of gene transcription" pptx
... astrocytes, microglial cells and endothelial cells may be potential sources of MIP-2 production in pathological states of the brain Stimulation of brain microvascular endothelial cells with tumor necrosis ... scavenging by curcuminoids J Pharm Pharmacol 1997, 49:105-107 Kang BY, Chung SW, Chung W, Im S, Hwang SY, Kim TS: Inhibition of interleukin-12 production in lipopolysaccharide-activated macrophages by ... curcumin Eur J Pharmacol 1999, 384:191-195 Kang BY, Song YJ, Kim KM, Choe YK, Hwang SY, Kim TS: Curcumin inhibits Th1 cytokine profile in CD4+ T cells by suppressing interleukin-12 production in...
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báo cáo hóa học: " A role for DNA-dependent activator of interferon regulatory factor in the recognition of herpes simplex virus type 1 by glial cells" pot
... HSV1 on Vero cells at 34°C MHV-68 viral stocks were prepared by infecting monolayer cultures of BHK-21 cells (ATCC, CCL-10) at a low viral MOI of 0.1 PFU per cell After 24 hour, the cells were ... difference from levels produced by unstimulated cells (p < 0.05) Figure DAI knockdown attenuates HSV-1-induced inflammatory cytokine production by murine glial cells Microglia and astrocytes were ... escape from recognition by adaptive immune cells Based on these observations, it appears likely that the production of cytotoxic substances by HSV-1 challenged glial cells could play a significant...
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báo cáo hóa học: " Neuropeptide Y inhibits interleukin-1b-induced phagocytosis by microglial cells" ppt
... and IL-1b-activated cells compared to untreated cells To visualize cell morphology, we labeled cells with the surface marker CD11b, which is known to be expressed by microglial cells [23] Interestingly, ... Immunocytochemistry Cells were fixed with 4% PFA (Sigma) and unspecific binding was prevented by incubating cells in a 3% BSA and 0.3% Triton X-100 solution (all from Sigma) for 30 min, at RT Cells were ... Barcelona, Spain) Cells were kept at 37°C in a 95% atmospheric air and 5% CO humidified atmosphere Numbers of viable cells were evaluated by counting trypan blue-excluding cells that were then...
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Báo cáo hóa học: " Hantaviruses and TNF-alpha act synergistically to induce ERK1/2 inactivation in Vero E6 cells" docx
... replication-dependent down-regulation of ERK1/2 by TULV, TOPV and SEOV, which was synergistically enhanced by TNF-α ERK1/2 inhibition was induced by TNF-α also in PUUV-infected cells ERK1/2 refers to prototype ... infected cells by immunoblotting of the nucleocapsid protein and quantification of released infectious virus Our results showed that virus replication was severely compromised in infected cells ... compared to viable cells) The treatment of Vero E6 cells with a high concentration of TNF-α resulted in a similar level of apoptosis and reduction of ERK1/2 activity compared to cells infected with...
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Báo cáo y học: "Indoleamine 2,3-dioxygenase-expressing dendritic cells are involved in the generation of CD4+CD25+ regulatory T cells in Peyer''''s patches in an orally tolerized, collagen-induced arthritis mouse model" ppt
... CD4+CD25- T cells into CD4+CD25+ T cells (Figure 5d) Our findings show that highly purified CD4+CD25- T cells can be converted into CD4+CD25+ Foxp3+ T cells by DCs from tolerized mice by CII-specific ... cells was 95% to 99% as evaluated by flow cytometry CD11c+ DCs (1 × 104 cells) isolated from Peyer's patch mononuclear cells of tolerized or CIA mice were cultured for days with CD4+CD25- T cells ... of antigen-specific CD4+CD25+ T cells by CD11c+ dendritic cells in tolerized mice Regulatory APCs may form a bridge between regulatory T cells and responder T cells, and this has been proposed...
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Báo cáo y học: "Immune regulation of bone loss by Th17 cells" doc
... osteoclastogenesis is inhibited by osteoprotegerin, a soluble decoy receptor for RANKL, which is also produced by a variety of cells including osteoblasts, synovial fibroblasts, B cells, and T cells [25-29] Osteoprotegerin-deficient ... IL-17+ Th cells that cause autoimmune arthritis J Exp Med 2007, 204:41-47 102 Aarvak T, Chabaud M, Miossec P, Natvig JB: IL-17 is produced by some proinflammatory Th1/Th0 cells but not by Th2 cells ... T-helper cells The source of the Th2 master differentiation factor IL-4 during the naïve to memory transition is controversial, but mature Th2 cells, natural killer T cells, basophils, and mast cells...
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báo cáo khoa học: "Upregulated expression of indoleamine 2, 3-dioxygenase in CHO cells induces apoptosis of competent T cells and increases proportion of Treg cells" pptx
... CD3+ T cells by RT-PCR analysis CD3+T cells alone and CD3+T cells co-cultured with CHO/EGFP cells were used as negative controls The value of the Foxp3 and b-actin gray scale ratios in CD3+ T cells ... with IDO+ CHO cells CD3+T cells and CD3+T cells co-cultured with CHO/EGFP cells were used as negative controls Cell lysates from T cells isolated from co-cultures with IDO+ CHO cells contained ... co-culture of CD3+T cells and IDO+ CHO cells, the apoptosis could not be induced (only 33.1 ± 4.87% of CD3+T cells were apoptotic) (Figure 2) Page of 10 cells co-cultured with CHO/EGFP cells were used...
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Báo cáo y học: "Differential induction of inflammatory cytokines by dendritic cells treated with novel TLR-agonist and cytokine based cocktails: targeting dendritic cells in autoimmunity" potx
... 5000 events were collected by gating hDCs defined by forward (FSC) and sidescatter (SSC) characteristics All histograms were gated for CD1a cells (70-95%) All our cells were CD14 negative (data ... was highest in the media from untreated control cells, showing that in a MLR reaction by itself, a certain pool of the T -cells develop into Th2 -cells However, none of the cocktails induced IL-4, ... non-adherent cells were washed in ice cold PBS Adherent cells were scraped off using a cell scraper and spun down Loading buffer was added to the cell pellet and lysates processed as described by the...
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Báo cáo y học: "Identification of proteases employed by dendritic cells in the processing of protein purified derivative (PPD)" pdf
... for Ag presentation by DC separations as before [24,25] Briefly, spleen cell suspensions were first depleted of B cells using Dynabeads conjugated with anti-mouse IgG Subsequently, T cells were ... hapten-like molecules by epidermal Langerhans cells J Leukoc Biol 1995, 57:891-896 Kapsenberg M, Teunissen M, Stiekema F, Keizer H: Antigen-presenting cell function of dendritic cells and macrophages ... mature murine dendritic cells is caused by subsets of incompletely matured cells J Immunol 1995, 155:93-99 Chain B, Kay P, Feldmann M: The cellular pathway of antigen presentation: Biochemical...
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Báo cáo y học: " Prime immunization with rotavirus VLP 2/6 followed by boosting with an adenovirus expressing VP6 induces protective immunization against rotavirus in mice" pps
... presence of RV antigen in fecal samples (A) was determined by a sandwich-ELISA Reduction in shedding (B) was calculated for each animal by comparing the area under the curve for each individual ... VLP2/6 was produced by expression of RV VP2 and VP6 simultaneously in Spodoptera frugiperda (Sf9) cells through recombinant baculovirus The recombinant baculovirus was generated by the Bac-to-Bac® ... manufacturer’s protocol RV VLP2/6 was purified by ultracentrifugation as described previously [49,50] Briefly, the supernatants of Sf9 cells infected by the recombinant baculovirus were collected...
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báo cáo khoa học: " A membrane-bound matrix-metalloproteinase from Nicotiana tabacum cv. BY-2 is induced by bacterial pathogens" pdf
... http://www.biomedcentral.com/1471-2229/9/83 Induction of NtMMP1 in wild type BY- 2 suspension cells Figure Induction of NtMMP1 in wild type BY- 2 suspension cells BY- 2 cells were treated with A tumefaciens, P syringae pv ... expression in BY- 2 cells The expression of NtMMP1 mRNA and NtMMP1 protein was monitored in wild type BY- 2 cells between days and 10 of a typical fermentation cycle The mRNA could be detected by Northern ... http://www.biomedcentral.com/1471-2229/9/83 Figure BY- 2 confocal laser scanning microscopy BY- 2 confocal laser scanning microscopy Tobacco BY- 2 cells stably transformed with NtMMP1-GFP were analyzed by confocal laser scanning...
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Báo cáo y học: " Protease-activated receptor 2 activation of myeloid dendritic cells regulates allergic airway inflammation" pptx
... receptor-2 expressed by lung epithelial cells J Immunol 2001, 167:1014-1021 Kauffman HF, Tomee JF, van de Reit MA, Timmerman AJ, Borger P: Protease-dependent activation of epithelial cells by fungal allergens ... strainer, and red blood cells were lysed with ACK lysis buffer (Invitrogen, Carlsbad, CA) Cells were washed with RPMI containing 10% FBS, counted and plated at 500,000 cells per well in a 96 well ... GM-CSF (10 ng/ml) on day On day 6, cells were treated with endotoxin-free PBS or GC frass (1 μg/ml) for 24 h Cells were washed, counted and resuspended at 2.5 × 106 cells/ ml Lewkowich et al Respiratory...
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