2 clinical application of bone engineering in omfs

... by incubation at 4C for 60 with 100 L of 0.1% gelatin in 20 mM TrisHCl (pH 7.4)-buffered saline (TBS) containing 10 mM CaCl2 and 1% BSA to prevent nonspecic binding of cells (7) After washing in ... Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore MARI DEZAWA Department of Stem Cell Biology and Histology, Tohoku University Graduate School of Medicine, ... further increasing the risks of bleeding, infection, and loss of viability of transplanted cells After infusion into the portal circulation, the transplanted cells move along the portal tracts into...

Ngày tải lên: 29/05/2014, 17:06

... arresting the growth of most kinds of cells, promote the proliferation of some types of cells There is increasing evidence that mild hypoxia acts as a potent regulator of various types of stem ... reported that hypoxia induces the transcription of HIF-1a mRNA, which increases the level of HIF-1a protein in the presence of continued hypoxia [37,38] Expression of HIF-1 (protein level) was markedly ... expression of HIF-1a protein showed an increase after infection with Ad–HIF-1a (Fig 3A) The total number of cells in NPCs infected with Ad–HIF-1a increased in comparison to those in the Ad–eGFP group...

Ngày tải lên: 30/03/2014, 04:20

... examine several clinical conditions in which increasing circulating EPC may play a role in response to injury Clinical Increase of Circulating EPC as a Response to Injury Tissue injury and hypoxia ... remodeling subsequent to administration of autologous bone marrow mononuclear cells [127-129] Original thoughts regarding the use of bone marrow stem cells in infarcts revolved around studies showing ... correcting for age, sex, and severity of illness [61] From an interventional perspective, transplantation of EPC into a rabbit model of acute lung injury resulted in reduction of leukocytic infiltrates...

Ngày tải lên: 18/06/2014, 15:20

... increasingly recognized as having diverse non-hematopoietic functions including production of angiogenic cytokines [48], and acting as an “innate” immune cell capable of rapidly differentiating into ... such as vitamins Page of 10 and minerals 4-5 days before trial initiation Two ml blood draws in heparinized Vacutainer tubes were collected by venipuncture before administration of StemKine supplementation ... post-injury healing processes For example, in a trial of post acute myocardial infarct patients, administration of G-CSF for days resulted in significant inhibition of pathological remodeling and improvement...

Ngày tải lên: 18/06/2014, 16:20

... screening visits or clinical investigations in addition to standard pre-transplant work-up Standardof-care examinations for patients on the Liver Transplant Waiting List will be performed, including ... active infection, or syphilis active infection History of any malignancy (including lymphoproliferative disease and hepatocellular carcinoma) except for squamous or basal cell carcinoma of the skin ... with increased risk of infections or renal dysfunction In view of the synergistic interplay of MSCs with mycophenolic acid, and because CNIs have been shown to abolish the beneficial effect of...

Ngày tải lên: 18/06/2014, 22:20

... screening visits or clinical investigations in addition to standard pre-transplant work-up Standardof-care examinations for patients on the Liver Transplant Waiting List will be performed, including ... active infection, or syphilis active infection History of any malignancy (including lymphoproliferative disease and hepatocellular carcinoma) except for squamous or basal cell carcinoma of the skin ... with increased risk of infections or renal dysfunction In view of the synergistic interplay of MSCs with mycophenolic acid, and because CNIs have been shown to abolish the beneficial effect of...

Ngày tải lên: 20/06/2014, 04:20

... superiority of intracoronary infusion of BMC over the infusion of CPC for improving global left ventricular function, but also corroborates our findings in the analysis of data according to initial ... Culminating in Acute Myocardial Infarction There are multiple stages in the atherosclerotic process culminating in thrombosis and acute myocardial infarction: initial formation of plaque in a ... administration of 7500 to 10,000 U of heparin and (in 89% of the cell-treated patients) a bolus of abciximab (0.25 mg per kilogram of body weight) Cells were infused into the vessel supplying...

Ngày tải lên: 27/06/2014, 00:20

... Immunofluorescence and TUNEL staining in vivo Free-floating sections (40 µm) were used for all in vivo experiments for TUNEL staining and combined immunofluorescence staining Detection of nuclear ... provide rapid in vitro screens for analyzing new therapies and discovering means of achieving selective protection or targeted killing In light of the ease of use of these in vitro and in vivo assays, ... decreasing series of ethanol in 90%, 70%, and 50% for each, followed by rinsing in distilled water After three rinses in TBS, the sections were exposed to To combine TUNEL staining with immunofluorescence...

Ngày tải lên: 06/08/2014, 18:21

... cultured in medium containing dexamethasone, isobutyl-methylxanthine and indomethacin About 10–30% of the OC cells were reproducibly induced toward the adipogenic lineage as early as weeks after induction ... biotinylated in the dark for 20 on ice The antibodies used are listed in Table After a washing step, second staining for biotin-conjugated monoclonal antibodies was done with streptavidin peridinin ... expression of genes for all tested osteogenic markers (Fig 6): alkaline phosphatase, bone sialoprotein and osteocalcin Discussion We could show that a defined population of MPCs resides within OC of...

Ngày tải lên: 09/08/2014, 01:23

... comparable levels of adhesion to unstimulated endothelial cells or matrix proteins such as laminin [8] What is the relevance of these findings in terms of our understanding of RA? Increased angiogenesis ... was certainly seen in the study of Herbrig and colleagues, who studied blood flow in the forearm following infusion of acetylcholine Vasodilatation was significantly reduced in the arms of RA patients ... circulating EPC numbers are reduced in RA and may lead to increased cardiovascular mortality This is an important issue in the context of RA, since an increased risk of cardiovascular disease, mainly...

Ngày tải lên: 09/08/2014, 07:20

... the amino-terminal two repeats of the death effector domain (DED), it lacks the carboxy-terminal half of the proteolytic domain Caspase 8L binds to the Fas-associating protein with death domain ... College of Rheumatology (ACR), and were in an inactive stage of disease defined as SLE disease activity index (SLEDAI) ≤4 [15,16] At the time of inclusion, 18 of 44 patients had a SLEDAI score of ... the in vitro binding of CPCs to fibronectin as a reflection of the capacity of CPCs to bind to damaged endothelium in vivo However, the overnight adhesion assay showed similar percentages of adhered...

Ngày tải lên: 09/08/2014, 10:20

... involvement of interferon-gamma (IFN-γ) via its induction of indoleamine 2,3-dioxygenase (IDO), an enzyme involved in the catabolism of tryptophan, an essential amino acid required for protein ... staining with Oil red O (bars = 40 μm); in osteogenesis, the matrix mineralization is shown by Alizarin red staining (bars = 40 μm); and in chondrogenesis, the accumulation of sulfated glycosaminoglycan-rich ... clearly showed adipocytes containing lipid droplets, matrix accumulation of sulfated glycosaminoglycans in cell pellets, and areas of mineralization in cultures maintained under adipogenic, chondrogenic,...

Ngày tải lên: 09/08/2014, 10:23

... 20 in a final volume of 100 µl of medium containing µg/ml polybrene Following transduction, cell aliquots were used for carrying out in vitro colony forming unit (CFU) assays, generation of macrophages, ... cells were cultured in vitro for an additional days, a rapid decline in the number of CD4/CD8 double positive cells was observed which coincided with a corresponding increase in single positive mature ... gene therapy strategies against HIV-1 infection depends on harnessing novel interfering genes for in vivo application in humans This requires collective utilization of stem cell gene transduction...

Ngày tải lên: 10/08/2014, 05:20

... neural stem cell differentiation into neuron while inhibiting the proliferation of NPCs in the early stage of neurogenesis In immunochemical staining, the number of nestin positive cells was decreased ... were diluted in blocking buffer and incubated for hrs at room temperature in the dark condition.), In some cases, nucleus was co-stained with DAPI (4’-6-diamidino-2-phenylindole) staining solution ... size of the brain by influencing the proliferation and differentiation properties of NPCs during developmental periods Regarding the effect of ethanol on NPCs, it inhibits the proliferation of...

Ngày tải lên: 10/08/2014, 05:21

... Mitogen-activated protein kinase inhibition induces translocation of Bmf to promote apoptosis in melanoma Cancer Res 2009, 69:1985-94 36 Yang J, Chai L, Liu F, Fink LM, Lin P, Silberstein LE, Amin HM, Ward ... isoforms is capable of enhancing and supporting primitive hematopoietic cells in the presence of combined cytokine stimulations Enhanced HSC/HPC activity by forced expression of Sall4 isoforms Next, ... molecules that are implicated in the Wnt/beta-catenin signaling pathway which is essential for HSC growth, including c-Myc, cyclin D1 and cyclin D2 Page of 14 Sall4 inhibits myeloid progenitor...

Ngày tải lên: 10/08/2014, 21:23

... Page of remained loosely attached to the bottom of the dish after washing steps The optical spectrum of one of such QD cluster outside EPCs is shown in Fig measured by using a wavelength scanning ... description in Ref [10] and references therein), which were octadecylamine coated so that they were not water soluble They were dissolved in chloroform and a same volume of a water solution containing ... similar blue shift of fluorescence peak of thiol-capped CdTe QDs within less than 10 of QD uptaking in living cells caused by surface photooxidation [16] The reported blue shift in CdTe QDs is much...

Ngày tải lên: 11/08/2014, 00:22

... marrow-derived prominin-1+ progenitors in the lung So far, we have identified two distinct populations of prominin-1 expressing cells in the healthy lung Bone marrow-derived prominin-1+lo progenitors ... potential of prominin-1+ progenitors during acute pulmonary inflammation We injected intratracheally bleomycin and, after days, sorted prominin-1 + cells from the inflamed lung and expanded them in ... we observed increasing proportions of CD45 expression within the whole prominin-1+ cell population (Figure 5A) 14 days after bleomycin instillation prominin-1+/CD45+ cell subset increased about...

Ngày tải lên: 12/08/2014, 14:20

... sample With this internal standard it was possible to calculate the amount of circulating EC per ml of blood In vivo experimental protocol including the intravenous injection of EPC All experiments ... tolerability of intraveinous application of EBM-2 (1 ml) alone turned out to be safe Intravenous application of EPC was performed 50 to 120 after reperfusion of the transplanted left lung Assessment of ... stomach, small intestine, colon, bone) were preserved in 10% PBS-buffered formalin solution as well as deep-frozen in liquid nitrogen and stored at -70°C Immunofluorescence staining of tissue specimens...

Ngày tải lên: 12/08/2014, 15:20

... clearance of Lin- PCs from the circulation In the pneumonia model, however, the clearance of LinPCs from the circulation at hours following infusion decreased (Figure 2) Interestingly, these findings ... and sequestration of progenitor cells Increased number of circulating progenitor cells has been shown in tissue injury animal models [26,27] as well as in clinical settings including burn, post-cardiac ... pneumonia lungs Few of these Lin- PCs migrated into the airspaces (approximately 3.1% of all the cells remaining in the lung), therefore approximately 14.6% of the initially infused Lin- PCs homed...

Ngày tải lên: 12/08/2014, 15:21

... biotin in terms of a clearly lower background All antibodies were diluted in PBS containing 5% bovine serum albumin The signal was developed by incubation with hydrogen peroxide and DAB (diaminobenzidine ... carbon dioxide in serum-free DMEM/F12 Nutmix (DMEM/F12; Invitrogen, Karlsruhe, Germany) containing 0.1% pronase E (Sigma-Aldrich, Taufkirchen, Germany) in a spinner flask for fine mincing and digestion ... groups (Figure 5a,b) Induction of the osteogenic lineage, as assessed by the detection of alkaline phosphatase, was more pronounced in CD166+ cells, indicating more progenitor cells in CD166+ than...

Ngày tải lên: 12/08/2014, 15:23