2 clinical application of bone engineering in omfs
liver stem cells methods and protocols
... by incubation at 4C for 60 with 100 L of 0.1% gelatin in 20 mM TrisHCl (pH 7.4)-buffered saline (TBS) containing 10 mM CaCl2 and 1% BSA to prevent nonspecic binding of cells (7) After washing in ... Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore MARI DEZAWA Department of Stem Cell Biology and Histology, Tohoku University Graduate School of Medicine, ... further increasing the risks of bleeding, infection, and loss of viability of transplanted cells After infusion into the portal circulation, the transplanted cells move along the portal tracts into...
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Báo cáo khoa học: Hypoxia-driven proliferation of embryonic neural stem ⁄ progenitor cells – role of hypoxia-inducible transcription factor-1a ppt
... arresting the growth of most kinds of cells, promote the proliferation of some types of cells There is increasing evidence that mild hypoxia acts as a potent regulator of various types of stem ... reported that hypoxia induces the transcription of HIF-1a mRNA, which increases the level of HIF-1a protein in the presence of continued hypoxia [37,38] Expression of HIF-1 (protein level) was markedly ... expression of HIF-1a protein showed an increase after infection with Ad–HIF-1a (Fig 3A) The total number of cells in NPCs infected with Ad–HIF-1a increased in comparison to those in the Ad–eGFP group...
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báo cáo hóa học:" Circulating endothelial progenitor cells: a new approach to anti-aging medicine?" docx
... examine several clinical conditions in which increasing circulating EPC may play a role in response to injury Clinical Increase of Circulating EPC as a Response to Injury Tissue injury and hypoxia ... remodeling subsequent to administration of autologous bone marrow mononuclear cells [127-129] Original thoughts regarding the use of bone marrow stem cells in infarcts revolved around studies showing ... correcting for age, sex, and severity of illness [61] From an interventional perspective, transplantation of EPC into a rabbit model of acute lung injury resulted in reduction of leukocytic infiltrates...
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Báo cáo hóa học: " Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects" potx
... increasingly recognized as having diverse non-hematopoietic functions including production of angiogenic cytokines [48], and acting as an “innate” immune cell capable of rapidly differentiating into ... such as vitamins Page of 10 and minerals 4-5 days before trial initiation Two ml blood draws in heparinized Vacutainer tubes were collected by venipuncture before administration of StemKine supplementation ... post-injury healing processes For example, in a trial of post acute myocardial infarct patients, administration of G-CSF for days resulted in significant inhibition of pathological remodeling and improvement...
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Báo cáo sinh học: "Safety and feasibility of third-party multipotent adult progenitor cells for immunomodulation therapy after liver transplantation–a phase I study (MISOT-I)" doc
... screening visits or clinical investigations in addition to standard pre-transplant work-up Standardof-care examinations for patients on the Liver Transplant Waiting List will be performed, including ... active infection, or syphilis active infection History of any malignancy (including lymphoproliferative disease and hepatocellular carcinoma) except for squamous or basal cell carcinoma of the skin ... with increased risk of infections or renal dysfunction In view of the synergistic interplay of MSCs with mycophenolic acid, and because CNIs have been shown to abolish the beneficial effect of...
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báo cáo hóa học:" Safety and feasibility of third-party multipotent adult progenitor cells for immunomodulation therapy after liver transplantation–a phase I study (MISOT-I)" pptx
... screening visits or clinical investigations in addition to standard pre-transplant work-up Standardof-care examinations for patients on the Liver Transplant Waiting List will be performed, including ... active infection, or syphilis active infection History of any malignancy (including lymphoproliferative disease and hepatocellular carcinoma) except for squamous or basal cell carcinoma of the skin ... with increased risk of infections or renal dysfunction In view of the synergistic interplay of MSCs with mycophenolic acid, and because CNIs have been shown to abolish the beneficial effect of...
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Transcoronary Transplantation of Progenitor Cells after Myocardial Infarction ppt
... superiority of intracoronary infusion of BMC over the infusion of CPC for improving global left ventricular function, but also corroborates our findings in the analysis of data according to initial ... Culminating in Acute Myocardial Infarction There are multiple stages in the atherosclerotic process culminating in thrombosis and acute myocardial infarction: initial formation of plaque in a ... administration of 7500 to 10,000 U of heparin and (in 89% of the cell-treated patients) a bolus of abciximab (0.25 mg per kilogram of body weight) Cells were infused into the vessel supplying...
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Báo cáo sinh học: " CNS progenitor cells and oligodendrocytes are targets of chemotherapeutic agents in vitro and in vivo" pdf
... Immunofluorescence and TUNEL staining in vivo Free-floating sections (40 µm) were used for all in vivo experiments for TUNEL staining and combined immunofluorescence staining Detection of nuclear ... provide rapid in vitro screens for analyzing new therapies and discovering means of achieving selective protection or targeted killing In light of the ease of use of these in vitro and in vivo assays, ... decreasing series of ethanol in 90%, 70%, and 50% for each, followed by rinsing in distilled water After three rinses in TBS, the sections were exposed to To combine TUNEL staining with immunofluorescence...
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Báo cáo y học: "Identification of subpopulations with characteristics of mesenchymal progenitor cells from human osteoarthritic cartilage using triple staining for cell surface markers" docx
... cultured in medium containing dexamethasone, isobutyl-methylxanthine and indomethacin About 10–30% of the OC cells were reproducibly induced toward the adipogenic lineage as early as weeks after induction ... biotinylated in the dark for 20 on ice The antibodies used are listed in Table After a washing step, second staining for biotin-conjugated monoclonal antibodies was done with streptavidin peridinin ... expression of genes for all tested osteogenic markers (Fig 6): alkaline phosphatase, bone sialoprotein and osteocalcin Discussion We could show that a defined population of MPCs resides within OC of...
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Báo cáo y học: "It’s all in the blood: circulating endothelial progenitor cells link synovial vascularity with cardiovascular mortality in rheumatoid arthritis" docx
... comparable levels of adhesion to unstimulated endothelial cells or matrix proteins such as laminin [8] What is the relevance of these findings in terms of our understanding of RA? Increased angiogenesis ... was certainly seen in the study of Herbrig and colleagues, who studied blood flow in the forearm following infusion of acetylcholine Vasodilatation was significantly reduced in the arms of RA patients ... circulating EPC numbers are reduced in RA and may lead to increased cardiovascular mortality This is an important issue in the context of RA, since an increased risk of cardiovascular disease, mainly...
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Báo cáo y học: "Reduced number and impaired function of circulating progenitor cells in patients with systemic lupus erythematosus" pot
... the amino-terminal two repeats of the death effector domain (DED), it lacks the carboxy-terminal half of the proteolytic domain Caspase 8L binds to the Fas-associating protein with death domain ... College of Rheumatology (ACR), and were in an inactive stage of disease defined as SLE disease activity index (SLEDAI) ≤4 [15,16] At the time of inclusion, 18 of 44 patients had a SLEDAI score of ... the in vitro binding of CPCs to fibronectin as a reflection of the capacity of CPCs to bind to damaged endothelium in vivo However, the overnight adhesion assay showed similar percentages of adhered...
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Báo cáo y học: "Human palatine tonsil: a new potential tissue source of multipotent mesenchymal progenitor cells" pdf
... involvement of interferon-gamma (IFN-γ) via its induction of indoleamine 2,3-dioxygenase (IDO), an enzyme involved in the catabolism of tryptophan, an essential amino acid required for protein ... staining with Oil red O (bars = 40 μm); in osteogenesis, the matrix mineralization is shown by Alizarin red staining (bars = 40 μm); and in chondrogenesis, the accumulation of sulfated glycosaminoglycan-rich ... clearly showed adipocytes containing lipid droplets, matrix accumulation of sulfated glycosaminoglycans in cell pellets, and areas of mineralization in cultures maintained under adipogenic, chondrogenic,...
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Báo cáo y học: " Lentiviral transduction of Tar Decoy and CCR5 ribozyme into CD34+ progenitor cells and derivation of HIV-1 resistant T cells and macrophages" pdf
... 20 in a final volume of 100 µl of medium containing µg/ml polybrene Following transduction, cell aliquots were used for carrying out in vitro colony forming unit (CFU) assays, generation of macrophages, ... cells were cultured in vitro for an additional days, a rapid decline in the number of CD4/CD8 double positive cells was observed which coincided with a corresponding increase in single positive mature ... gene therapy strategies against HIV-1 infection depends on harnessing novel interfering genes for in vivo application in humans This requires collective utilization of stem cell gene transduction...
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Báo cáo y học: "Prenatal exposure of ethanol induces increased glutamatergic neuronal differentiation of neural progenitor cells" pot
... neural stem cell differentiation into neuron while inhibiting the proliferation of NPCs in the early stage of neurogenesis In immunochemical staining, the number of nestin positive cells was decreased ... were diluted in blocking buffer and incubated for hrs at room temperature in the dark condition.), In some cases, nucleus was co-stained with DAPI (4’-6-diamidino-2-phenylindole) staining solution ... size of the brain by influencing the proliferation and differentiation properties of NPCs during developmental periods Regarding the effect of ethanol on NPCs, it inhibits the proliferation of...
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Báo cáo y học: " Enhanced self-renewal of hematopoietic stem/progenitor cells mediated by the stem cell gene Sall4" doc
... Mitogen-activated protein kinase inhibition induces translocation of Bmf to promote apoptosis in melanoma Cancer Res 2009, 69:1985-94 36 Yang J, Chai L, Liu F, Fink LM, Lin P, Silberstein LE, Amin HM, Ward ... isoforms is capable of enhancing and supporting primitive hematopoietic cells in the presence of combined cytokine stimulations Enhanced HSC/HPC activity by forced expression of Sall4 isoforms Next, ... molecules that are implicated in the Wnt/beta-catenin signaling pathway which is essential for HSC growth, including c-Myc, cyclin D1 and cyclin D2 Page of 14 Sall4 inhibits myeloid progenitor...
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báo cáo khoa học: "Optical characterization of colloidal CdSe quantum dots in endothelial progenitor cells" pptx
... Page of remained loosely attached to the bottom of the dish after washing steps The optical spectrum of one of such QD cluster outside EPCs is shown in Fig measured by using a wavelength scanning ... description in Ref [10] and references therein), which were octadecylamine coated so that they were not water soluble They were dissolved in chloroform and a same volume of a water solution containing ... similar blue shift of fluorescence peak of thiol-capped CdTe QDs within less than 10 of QD uptaking in living cells caused by surface photooxidation [16] The reported blue shift in CdTe QDs is much...
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Báo cáo y học: " Profibrotic potential of Prominin-1+ epithelial progenitor cells in pulmonary fibrosis" docx
... marrow-derived prominin-1+ progenitors in the lung So far, we have identified two distinct populations of prominin-1 expressing cells in the healthy lung Bone marrow-derived prominin-1+lo progenitors ... potential of prominin-1+ progenitors during acute pulmonary inflammation We injected intratracheally bleomycin and, after days, sorted prominin-1 + cells from the inflamed lung and expanded them in ... we observed increasing proportions of CD45 expression within the whole prominin-1+ cell population (Figure 5A) 14 days after bleomycin instillation prominin-1+/CD45+ cell subset increased about...
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Báo cáo y học: " Peripheral infusion of rat bone marrow derived endothelial progenitor cells leads to homing in acute lung injury" pps
... sample With this internal standard it was possible to calculate the amount of circulating EC per ml of blood In vivo experimental protocol including the intravenous injection of EPC All experiments ... tolerability of intraveinous application of EBM-2 (1 ml) alone turned out to be safe Intravenous application of EPC was performed 50 to 120 after reperfusion of the transplanted left lung Assessment of ... stomach, small intestine, colon, bone) were preserved in 10% PBS-buffered formalin solution as well as deep-frozen in liquid nitrogen and stored at -70°C Immunofluorescence staining of tissue specimens...
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Báo cáo y học: "Sequestration and homing of bone marrow-derived lineage negative progenitor cells in the lung during pneumococcal pneumonia" ppsx
... clearance of Lin- PCs from the circulation In the pneumonia model, however, the clearance of LinPCs from the circulation at hours following infusion decreased (Figure 2) Interestingly, these findings ... and sequestration of progenitor cells Increased number of circulating progenitor cells has been shown in tissue injury animal models [26,27] as well as in clinical settings including burn, post-cardiac ... pneumonia lungs Few of these Lin- PCs migrated into the airspaces (approximately 3.1% of all the cells remaining in the lung), therefore approximately 14.6% of the initially infused Lin- PCs homed...
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Báo cáo y học: "Relative percentage and zonal distribution of mesenchymal progenitor cells in human osteoarthritic and normal cartilage" potx
... biotin in terms of a clearly lower background All antibodies were diluted in PBS containing 5% bovine serum albumin The signal was developed by incubation with hydrogen peroxide and DAB (diaminobenzidine ... carbon dioxide in serum-free DMEM/F12 Nutmix (DMEM/F12; Invitrogen, Karlsruhe, Germany) containing 0.1% pronase E (Sigma-Aldrich, Taufkirchen, Germany) in a spinner flask for fine mincing and digestion ... groups (Figure 5a,b) Induction of the osteogenic lineage, as assessed by the detection of alkaline phosphatase, was more pronounced in CD166+ cells, indicating more progenitor cells in CD166+ than...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25