2 cartilage cells isolated for tissue engineering

... progenitor cells; (2) Hepatic differentiation from stem cells; (3) Bile ductal cell formation from stem cells; (4) Liver stem cells and hepatocarcinogenesis; and (5) Application of liver stem cells for ... stem cells in repair of liver injuries Several types of stem cells, such as embryonic stem (ES) cells, induced pluripotent stem (iPS) cells, haematopoietic stem cells, and mesenchymal stem cells, ... from nonparenchymal cells, including stellate cells, endothelial cells, and hemopoietic cells Various factors, which may be produced by nonparenchymal cells, could be assayed for their effects...

Ngày tải lên: 29/05/2014, 17:06

... NPCs T Zhao et al medium, and cells were exposed to hypoxia for days The cells were then fixed with 4% paraformaldehyde at °C for h For BrdU immunohistochemistry, the cells were pretreated with m ... stem cells derived from the embryonic mesencephalon (E13.5) were used in the present study Generally, neural stem cells were grown to form as neurospheres in vitro The ability of stem cells to form ... the indicators for proliferation of neural stem cells in vitro The passaged neurospheres were dissociated into single cells and planted in four-well plates at a density of · 104 cells per well,...

Ngày tải lên: 30/03/2014, 04:20

... cytometric analysis of cells double-staining for VEGFR2 and CD34 was performed with samples extracted at the indicated timepoints Y-axis represents percentage double positive cells from cells Page of ... (page number not for citation purposes) Journal of Translational Medicine 2009, 7:106 types have been ascribed to cells with EPC activity, one study demonstrated monocyte-like cells that expressing ... increased numbers of CD34 positive cells in 16 post infarct patients on day as compared to controls The rise in CD34 cells correlated with ability to differentiate into cells morphologically resembling...

Ngày tải lên: 18/06/2014, 15:20

... resuspended cells and refrigerated in the dark for 15 (4-8) C Cells were washed in ml of PBS with 0.5% BSA and resuspended in 100 ul of buffer for analysis Flow cytometry was performed using ... Vancouver, Canada) Cells were plated at a concentration million cells per well for days For each subject colonies were plated in triplicate Colonies represented clusters of more than 50 cells circumscribed ... total number of cells counted cells being 30,000 per sample The percentage of CD133 and CD34 positive cells was calculated based on the measured number of leukocytes (CD45-positive cells) Quantification...

Ngày tải lên: 18/06/2014, 16:20

... the Eurotransplant area, for instance, only 1631 transplant livers were available for 2641 patients on the waiting list in 2009 [12] To overcome this shortage, criteria for the acceptance of donors ... growth factors EGF and PDGF Cells display a linear expansion rate to 65 population doublings or greater before senescence Doubling times average 20 hours during expansion Cells are used after 30 ... protocol therefore calls for a CNI-free, “bottom-up” immunosuppressive regimen combined with the MAPC infusions “Bottom-up” immunosuppression Current standard clinical protocols for post-transplant...

Ngày tải lên: 18/06/2014, 22:20

... the Eurotransplant area, for instance, only 1631 transplant livers were available for 2641 patients on the waiting list in 2009 [12] To overcome this shortage, criteria for the acceptance of donors ... growth factors EGF and PDGF Cells display a linear expansion rate to 65 population doublings or greater before senescence Doubling times average 20 hours during expansion Cells are used after 30 ... protocol therefore calls for a CNI-free, “bottom-up” immunosuppressive regimen combined with the MAPC infusions “Bottom-up” immunosuppression Current standard clinical protocols for post-transplant...

Ngày tải lên: 20/06/2014, 04:20

... of Progenitor Cells tween 18 and 80 years of age were eligible for For patients assigned to receive CPC, mononuclear inclusion in the study if they had had a document- cells were isolated by Ficoll ... rehospitalization for heart failure (4) (9) 0.20 Death, MI, stroke, rehospitalization for heart failure, or ventricular tachycardia (13) (15) 0.07 Cumulative events, before or after discharge * For analysis ... catheterization procedure For patients undergoing bone marrow aspiration, potential bleeding complications were assessed During hospitalization, telemetry was routinely performed for 24 hours after...

Ngày tải lên: 27/06/2014, 00:20

... neural cells (rat) 100 120 Primary neural cells (human) 20 40 60 80 Percent survival 100 120 Cancer cells Figure Primary CNS cells are more vulnerable to BCNU and cisplatin than are cancer cells Cells ... has been studied for irradiation, and demonstrate toxicity of chemotherapeutic agents for glial progenitor cells and for oligodendrocytes, as well as for the hippocampal precursor cells that have ... h, cells were exposed to increasing drug concentrations of BCNU (5-200 µM) for h, cisplatin (0.1-100 µM) for 20 h or cytarabine (0.01 µM to µM) for 24 h Cells were then allowed to recover for...

Ngày tải lên: 06/08/2014, 18:21

... cytometry analysis of cells Either isolated cells from OC were directly used for flow cytometric analysis or cells were used after adherence and cultivation, as described above Cells were washed ... scatter the fresh isolated OC cells were a heterogeneous population The majority of the cells stained negative for CD9, CD90 and CD166 The proportion of CD9+/CD90+/CD166+ triple positive cells was only ... Germany) Dead cells were excluded by propidium iodide (Sigma) staining Cells were gated on forward and side scatter to exclude debris and cell aggregates To calculate the percentages of cells staining...

Ngày tải lên: 09/08/2014, 01:23

... of bone marrow-derived mononuclear cells Indeed, the first description of EPC showed that peripheral bloodderived CD34-positive cells integrated into newly formed vessels in an in vivo model ... and impaired function of endothelial progenitor cells Ann Rheum Dis 2005 [Epub ahead of print] Asahara T, Kawamoto A: Endothelial progenitor cells for postnatal vasculogenesis Am J Physiol Cell ... the cardiovascular field in recent years about the potential for mobilising bone marrow-derived EPC to increase neovascularisation for the treatment of ischaemic disease [9,10] The awareness that...

Ngày tải lên: 09/08/2014, 07:20

... on day 14 Cells were dissociated with Accutase (PAA Laboratories GmbH, Cölbe, Germany) Cells from 10 patients and 10 controls were resuspended in DMEM (BioWhittaker) at × 105 cells/ ml for the migration ... Shapiro-Wilk test for normality; Student's t-test was used for normally distributed data, Mann-Whitney U for non-parametric data Pearson's correlation coefficient was used to test for relations between ... potential, migratory capacity and cluster formation capacity on Matrigel After seven days of culture, central colonies of cells surrounded by emerging cells were formed The number of these CFU was decreased...

Ngày tải lên: 09/08/2014, 10:20

... described previously [27] For adipogenic differentiation, cells were seeded into six-well tissue culture plates at a density of 20,000 cells per square centimeter and treated for weeks with adipogenic ... mononuclear cells (PBMCs) (105 cells) were incubated for days with either μg/mL PHA or allogeneic stimulating PBMCs (1 × 105 cells) with or without bone marrow-derived mesenchymal progenitor cells ... antigen-specific proliferation (both for naive and memory T cells) , cytotoxic T-lymphocyte formation, IFN-γ production by Th1 cells, and interleukin-4 production by Th2 cells [39,40] The ability to decrease...

Ngày tải lên: 09/08/2014, 10:23

... CD34+ cells were differentiated into macrophages in vitro in cytokine medium Cells were stained for the macrophage surface marker CD14 using CD14-PE antibodies Cells were analyzed by FACS for CD14 ... human CD34+ cells (>90% pure) were used for vector transductions A representative FACS profile of purified CD34+ cells is shown in Fig 2A Transduction efficiency, as determined by FACS for EGFP ... and Tar-CCR5Rz transduced CD34+ cells When these cells were cultured in vitro for an additional days, a rapid decline in the number of CD4/CD8 double positive cells was observed which coincided...

Ngày tải lên: 10/08/2014, 05:20

... PA) were washed and fixed with 4% paraformaldehyde at 4°C for hrs The cells were treated with 0.3% Triton X-100 for 15 at room temperature and were blocked for 30 with blocking buffer (1% BSA, ... ethanol treatment, cells grown in 96-well plate were incubated at 37°C for 24 hrs with 10 μM of BrdU labeling solution After removing BrdU labeling solution, cells were fixed for 30 at room temperature ... http://www.jbiomedsci.com/content/17/1/85 brain cells which may cause structural deformities or deficits within the brain Neural stem/progenitor cells (NPCs) are selfrenewable cells in the CNS NPC is able to...

Ngày tải lên: 10/08/2014, 05:21

... genes in cultured LSK cells Murine Lin-Sca-1+ cells were infected with lentiviruses each containing Sall4 isoform or GFP control, cultured for days, and GFP+ LSK cells were isolated by FACS Total ... × 105 cells per mL were prepared and 0.3 mL of cells were added to mL of MethoCult® medium for duplicate cultures 1.1 mL of cells was dispensed per 35 mm dish The cells were incubated for 8-12 ... transduced SALL4 isoforms to mouse LSK cells and then performed syngeneic transplantations to allow for long-term followup As shown in Table 1, no MPN-like phenotypes were exhibited for more than 11...

Ngày tải lên: 10/08/2014, 21:23

... measurement [11] The cells were incubated with QD625 for 30 hours After QD incubation the cells were washed with phosphate-buffered saline (PBS, pH 7.2), fixed with 4% paraformaldehyde for 10 minutes ... notes on endothelial progenitor cells: ways to escape from the knowledge plateau Atherosclerosis 2008, 197:496-503 Kawamoto A, Losordo DW: Endothelial progenitor cells for cardiovascular regeneration ... They were dissolved in chloroform and a same volume of a water solution containing 3-mercaptopropionic acid (3-MPA) (1 mol/L = M) was then added under vigorous stirring for hours after which QDs...

Ngày tải lên: 11/08/2014, 00:22

... stem and progenitor cells isolated from the lung [11,12] or from other tissues [13-15] have the capacity to differentiate into pulmonary epithelial cells In addition, these cells exhibit anti-inflammatory ... analysis was performed on frozen tissue sections and cells cultured on gelatincoated cover slips as described previously [12] For prominin-1 detection, frozen sections and cultured cells were stained ... + /CD45+ cells out of all analyzed cells in the lung tissue following bleomycin treatment (C) FS - forward scatter, Iso- isotype control Bars represent mean ± SD from individual lung tissues Blyszczuk...

Ngày tải lên: 12/08/2014, 14:20

... occurred in damaged lung tissue after the setting of severe tissue injury, as previously shown in part in an abstract [58] As these cells are suggested to be important for repairing tissue damage, are ... dead cells, and the signal generated is directly proportional to the number of cells [62] After h of incubation, medium was aspirated from adherent cells without disturbing formazan crystals formed ... been made on rare engrafted cells, where circulating blood cells, dead cells, cell fusion, or artifacts like autofluorescence might lead to misinterpretation Therefore, the reconstitution of...

Ngày tải lên: 12/08/2014, 15:20

... hematopoietic stem cells from the BM [11,12] Recent studies have shown that these BM-derived stem cells or progenitor cells have the ability to differentiate into cells that repopulate damaged tissues in ... Cytomation Inc., Fort Collins, CO) Typically, 200,000 events for MNCs and 400,000 events for BMCs were acquired and the frequency of QD-positive cells was measured The numbers of donor cells in circulation ... preferential sequestration of these cells in inflamed lung tissues To determine homing of BM-derived cells to inflamed lung tissues, we investigated the localization of these cells at 24 hours after transfusion...

Ngày tải lên: 12/08/2014, 15:21

... approaches use markers already successfully described for other tissues (for example, bone marrow) However, MPCs isolated from different tissues may not show the same immunophenotype Possible ... Germany) Cells were gated using forward and side scatters to exclude debris and cell aggregates Immunohistochemistry of isolated osteoarthritis and normal chondrocytes Cells were grown for to days ... CD166+ cells from 10% before up to approximately 87% after separation (Figure 4a2,b2) The negative fraction contained less than 1% CD166+ cells (Figure 4c2) To obtain sufficient numbers of cells for...

Ngày tải lên: 12/08/2014, 15:23