2 amniotic fluid and placenta in developmental biology

... following a 5-minute incubation at 37°C with 0.05% trypsin/ethylenediaminetetraacetic acid (EDTA) (Gibco) and replated MSCs of C57BL/6 origin were kindly provided by Darwin J Prockop and Catherine ... injected intraperitoneally into each mouse After hours, mice were sacrificed and lymph nodes (axillary, inguinal, and mesenteric) and spleens were harvested Single-cell suspensions were obtained ... supporting [11,12] and refuting [8,11] a role for these two T-cell inhibitors in T-cell proliferation inhibition In addition, we could demonstrate that IL-17, in synergy with IFN-g, can induce...

Ngày tải lên: 12/08/2014, 11:23

... within the scaffolds This was confirmed by von Kossa staining, H&E staining and immunostaining with osteonectin and alkaline phosphatase (ALP) There was no evidence of teratoma formation In hFOB ... time point: H&E staining and von Kossa staining of sections showing scaffolds devoid of cells 43 Figure At week time-point: (a) H&E staining showing newly formed tissue within the ... scaffold (arrow showing scaffold remains) (b) von Kossa staining and counterstaining with nuclear fast red showing deposition of mineralized tissue in the area marked and shown in higher magnification:...

Ngày tải lên: 09/10/2015, 11:24

... containing genes upregulated in EB (node C) included the genes HAND1, HOXA1, HOXB2, MSX1, MSX2, MEIS1, FGF9 and FREM1 which are involved in morphogenesis and development [36-39] This node also included ... reported in human [16,17] and mouse embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs ... contained the genes that were upregulated in both hES cells and EB, node B contained the genes upregulated in hES cells only, node C contained the genes upregulated in EB only, and node D contained...

Ngày tải lên: 18/06/2014, 15:20

... malignant glioma and associated with tumor invasive factors, uPAR and RhoC Int J Cancer 2009 30 Ma L, Teruya-Feldstein J, Weinberg RA: Tumour invasion and metastasis initiated by microRNA-10b in breast ... serum (FCS) (Invitrogen), 100 U/mL penicillin G, 0.2 mg/mL streptomycin, and 500 ng/mL insulin HCC cells were isolated from DENinduced rat liver carcinomas Briefly, tumor nodules in Page of 10 ... cells induced by chemical carcinogens Since the HCC cancer cells and fetal liver cells used in our study originated from the same inbred rat strain, the SP fractions enriched by screening both...

Ngày tải lên: 10/08/2014, 10:20

... 500 time [min] Values of PPIX-fluorescence in normal findings, findings ≤ mild dysplasia and findings ≥ moderate dysplasia plotted against the Figure time between 5-ALA application and spectroscopy ... spectroscopy Values of PPIX-fluorescence in normal findings, findings ≤ mild dysplasia and findings ≥ moderate dysplasia plotted against the time between 5-ALA application and spectroscopy Fitted curves ... and the intensity of the calculated peak fluorescence values with discriminating differences between normal and pathologic findings As reported in other studies [6,9,17], there is always an increase...

Ngày tải lên: 12/08/2014, 15:20

... caused by infectious bovine rhinotracheitis virus in calves in Hungary Acta Vet Acad Sci Hung 1969, 19:145-151 Moretti B, Orfei Z, Mondino G, Persechino A: Infectious bovine rhinotracheitis, clinical ... sulfolipocyclodextrin in a squalane -in- water and water -in- mineral oil emulsions for BHV-1 vaccines in cattle Vaccine 2000, 19:132-41 32 Reed CJ, Muench HA: A simple method of estimating fifty per cent end points ... histological examination, the samples were fixed in 10% neutral buffered formalin, prepared by routine methods for histology, paraffin embedded, sectioned at μm and stained with hematoxylin and eosin The...

Ngày tải lên: 12/08/2014, 18:22

... stability and toxicity of PANs, and further investigated the sustained release behavior in vivo of drug loading in PANs PA was firstly synthesized and characterized by FT-IR and 1H NMR, and then ... novel hydrophobized polysaccharide/oncoprotein complex vaccine induces in vitro and in vivo cellular and humoral immune responses against HER2-expressing murine sarcomas Cancer Res 58:3385–90 Gustafson, ... and 4) Figure shows the microscopic examination of major organs including a b heart, liver, spleen, lung, and kidney sections stained with H&E Pathological changes in the major organs including...

Ngày tải lên: 23/04/2013, 21:38

... acrylamide in the separating and stacking gels, respectively In order to estimate the ratio between the amounts of UP12 and the total amount of protein in the extracts, a series of samples containing ... members of this family including UspA itself (Fig 2), and one larger protein consisting of two UspA domains in tandem [17] The small members of this family are proteins of 142–144 amino-acids long; ... according to the manufacturer’s instructions The protein was eluted with buffer K containing M imidazole and cleaved by thrombin (Novagen) at room temperature for 17–20 h, using a protease/protein...

Ngày tải lên: 22/02/2014, 07:20

... protein, in binding porphyrins It is not known how haem is transported intracellularly in bacteria, and the mechanisms by which haem is incorporated into soluble and membrane-bound proteins inside ... compounds being taken up into the cell and incorporated into vital haem proteins instead of haem [5] We show here that haem analogues are indeed taken up and can be incorporated into haem proteins, ... transiently bound to specific protein factors in cells Little is known about intracellular haem transport and how haem is incorporated into proteins in vivo to form haem proteins in various subcellular locations...

Ngày tải lên: 06/03/2014, 11:20

... concentration of Gal3p in the wild-type thus reducing the protein expression as in Model III This confirms the evidence obtained through gel filtering and cross-linking that Gal3p is monomeric in solution ... one binding site (A) and genes with two binding sites (B) Solid circles and solid triangles denote experimental data for a-galactosidase expression level for genes with one binding site and for ... observation given the constraints of total component concentrations and binding constants Discussion Studies in molecular biology have enumerated various interactions resulting in a complex regulatory...

Ngày tải lên: 07/03/2014, 16:20

... selenocysteine residue in a C-terminal Sel-tag [18] Der p carrying the Sel-tag had an intact core sequence and maintained allergen-specific IgE-binding epitopes and the use of a Sel-tag enabled labelling ... lymph node proteins revealed a radioactive band of  25 kDa in liver and bands of  30 and 56 kDa in thoracic lymph nodes (Fig 5C) No radioactive protein bands could be detected in spleen samples ... deposited in the airways of mice and metabolized The labelling method involves the incorporation of a selenocysteine residue and the gamma-emitter selenium-75 (75Se) within an engineered C-terminal...

Ngày tải lên: 07/03/2014, 21:20

... promoter and terminator, and into vectors for in vitro transcription and translation An Arabidopsis line (psag-1.4 [5], termed DG in this report), lacking PSI-G due to a transposon footprint in exon ... after 0, 30 and 60 digestion with trypsin, probed with antibodies directed against PSI-G, PSI-K (intrinsic membrane protein, stromal loop), PSI-O (intrinsic membrane protein, luminal loop) and PSI-D ... shown) In conclusion, we have shown using both in vitro and in vivo methods, that PSI-G adopts a topology in the thylakoid membrane with the loop facing the stroma and its N- and C-termini facing...

Ngày tải lên: 07/03/2014, 21:20

... liver and lung indicating that the amount of p53 protein was significantly increased in the liver and lung from vitamin A-deficient rats that in controls c-Jun protein was significantly decreased in ... expression was similar in control, vitamin A-deficiency and hypervitaminosis (Fig 5) rats An antibody against a protein unrelated to vitamin A was used as a mock control and binding was not observed ... [27] In rats Fig Western blot analysis of p53 and c-Jun in liver and lung Total protein extracts were obtained as described in experimental procedures A single band of about 53 kDa was detected in...

Ngày tải lên: 08/03/2014, 08:20

... use of mutants and inhibitors acting on the same biosynthetic points has proved to be a useful way of distinguishing the in uence of direct effects, i.e sterol alteration, and indirect effects ... lipid intermixing The reaction was terminated by adding the stopping reagent used for phosphate determination Consumption of substrate by the H+-ATPase was less than 15% under any conditions Kinetic ... correlation (r ¼ 0.98) was observed, and this was independent of the kind of genetic lesion or inhibitor used, suggesting that this activating effect was indeed caused by the fatty acid/lipid...

Ngày tải lên: 08/03/2014, 22:20

... vector containing three tandem repeat regions of miRNA23 binding sequence (reprinted with permission [11]) information for understanding the miRNA biogenesis related to human diseases Imaging miRNA ... diseases, in vivo In addition, the bioimaging techniques for miRNAs based on molecular imaging methods could be applied as target imaging indicators to help understand the developmental process, in ... Several reporter-based imaging probes, including luciferase for optical imaging and sodium iodide symporter and herpes simplex virus 1-thymidine kinase for radionuclide imaging, have been widely used...

Ngày tải lên: 16/03/2014, 01:20

... al In vivo degradation of NOS and HSP90 by calpain higher than in aorta, resulting in a much higher HSP90 to NOS ratio in brain (Fig 1C) A Calpain activation in rat brain and aorta following ... HSP90 are crucial in determining the in vivo resistance of NOS and HSP90 to calpain degradation Results Levels of HSP90 and NOS isozymes in rat brain and aorta The level of HSP90 and the type of ... protein) of the crude homogenate were incubated in a total volume of 250 lL in buffer A containing mm NADPH, 200 mm calmodulin, 20 lm tetrahydrobiopterin, lm FAD, lm FMN, lm l-arginine and 925...

Ngày tải lên: 30/03/2014, 04:20

... both in vitro and in vivo The two main VEGF receptors are VEGFR-1 or fms-like tyrosine kinase (Flt-1) and VEGFR-2 or fetal liver kinase (Flk-1) also known as kinase-insert domain-containing receptor ... neutral-buffered formalin solution and then embedded in paraffin All tissues were cut in μm sections and slides were counterstained with a standard mixture of hematoxylin and eosin [4] Quantitative ... biological properties of VEGF and shows the ability to induce capillary formation and organization in vitro [9], and showed to be active in gastric ulcer healing in rodents when administered either orally...

Ngày tải lên: 18/06/2014, 15:20

... ferently, using the anti-ErbB-2 antiserum cross reacting with the murine receptor resulted in a faint stain of the cytoplasm and rarely in staining of the membrane of the tumor cells (Figure 3B) In the ... were defined as negative Samples showing moderate (+) to strong (2+/3+) signals by staining them with anti hTid antibodies and 3+ or 2+/Fish+ stain using antibodies against ErbB-2 were defined as ... luminal B subtype) and 34 ErbB-2 negative tumors (16 luminal A, luminal B and 10 of the triple negative subtype) as well as in metastatic lesions over expressing the TRK receptor by staining...

Ngày tải lên: 18/06/2014, 16:20

... demonstrated increased strain in the ATFL with increasing plantarflexion, inversion and internal rotation Strain in the CFL was found to increase in dorsiflexion and inversion Ozeki et al.[7] investigated ... in this type of injury, as shown in several reports.[4,18,19] This notion was supported by numerous in- vitro studies which investigated the changes in ATFL and CFL strain in response to loading ... complaints of ankle pain, previous history of ankle injury requiring treatment, systemic disease, or contraindication for MR imaging Prior to MR scanning, both ankles, including the scanned and...

Ngày tải lên: 20/06/2014, 01:20