... the minor groove of PPRE consensus sequence interacts with the DNA-binding domains of PPARγ and RXRα and shields the highly polar side chains of the interacting residues (Asn 160 from PPARγ, and ... (Droge, 2002) Mitochondrialdiseases due to mutations in nDNA and mtDNA encoding for mitochondrial proteins are complex, and are confounded by a heterogeneous mix of clinical symptoms and inheritance ... effect would in turn cause mitochondrial injury, initiating a vicious cycle leading to cellular demise (Brown & 24 Borutaite, 2002) The critical importance of this enzyme in maintaining mitochondrial...
... resulted in assay outcomes that were identical with those obtained in the absence of heparin (data not shown) To gain insights into which rate constants (k506 and k¢306 in intact FVa and k306 in FVaint) ... directed against the FVa heavy chain Amino acids known to be part of the heparin-binding site in these three crystallized protein–heparin complexes were given as starting point for the docking search ... constants obtained by fitting all time courses to the random-order cleavage model as described in Materials and methods, under the constraints that k306 is minimally in uenced by heparin and using k¢306...
... being minimal the trapping of two main-chain conformations and increased B-factors at 295 K suggests that the presence of the coordinating Lys in some way can in uence the dynamics of the ligand ... basic f-amino group of the coordinating K100, with the H-bonded water molecule found in the X-ray structure increasing the electron donating power of the coordinating amino group which in turn ... prominent being the absence of the 695-nm band, indicative of Met-Sdiron coordination Upon titrating increasing amounts of guanidinium hydrochloride (GdmHCl) the protein unfolds resulting in the perturbation...
... preparation and delipidation of a bovine liver 38-kDa protein exhibiting EPT activity [25], a protein comigrating in SDS/PAGE was revealed by amino-acid sequence analysis The determined N-terminal sequence ... bovine D14-SR, regions of the deduced amino-acid sequence corresponding to the N-terminal and V8 peptide sequences determined in the sequencing experiments of the protein puri®ed from bovine ... protein from bovine liver ER with a high degree of identity with both human SR-1 and human LBR A cDNA encoding this protein was identi®ed by bovine expressed sequence tag (EST) analysis, cloned, and...
... medium containing 10% fetal bovine serum, 1% penicillin and 1% streptomycin Cells were loaded with DBZTC (10 lm, Hepes, pH 7.40) by incubation at 37 °C for 15 and showed negligible intracellular ... macrophages were cultured in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum, 1% penicillin, and 1% streptomycin at 37 °C (w/v) in a 5% CO2 ⁄ 95% air incubator MCO-15AC (SANYO, ... generated by reacting H2O2 with Co2+ or NaOCl, respectively Peroxynitrite (ONOO–) and nitric oxide (NOÆ) were obtained from 3morpholinosydnonimine hydrochloride (SIN-1), and 3-(aminopropyl)-1-hydroxy-3-isopropyl-2-oxo-1-triazene,...
... both the heme-binding sites (CxxCH), in addition to the amino acids coordinating the calcium ion present in the interface domain of the solved CCPs are positionally conserved in MCA2590 It is ... dehydrogenase (MeDH) in the soluble fraction and the outer membrane proteins MopB and MopE in the 6328 A B Fig SDS ⁄ PAGE (A) and protein immunoblot analyses (B) of proteins obtained during the fractionation ... periplasmic protein [18], hence indicating that the cells remained undisrupted during the extraction procedure and thereby avoiding leakage of proteins from the periplasm Protein immunoblot using antibodies...
... Malaysia Singapore Indonesia Access Point Exchange Point TEIN APII Testbed Australia Int’l ICT, IR, Asia Pacific Information Infrastructure At APEC Summit2 in Bogor (1994), APII was introduced ... France, Finland, EC Laos, Cambodia, Myanmar, Malaysia, Brunei, Vietnam, Singapore, Indonesia, Philippine, Thailand, ASEM ASEAN Int’l ICT, IR, 27 Europe-Asia Link to SEA Korea France TEIN II Link (34Mbps) ... (2001) Int’l ICT, IR, APII Testbed: Cable Networks * Korea-Japan Started with 2Mbps in April 1998 Expanded to 8Mbps in July 1999 Increased to 1Gbps in Feb 2003 * Korea-Singapore Deployed 2Mbps in...
... of protein complexes of intrinsic PMLA and nucleic acids are ÔtunedÕ for optimal function in the plasmodium and that an unscheduled increase in PMLA by injection perturbs this tuning and causes ... are indicated (mutant white strain LU897 · LU898, solid lines in Fig 2) showed a greater increase in size than the high PMLA producer (yellow strain M3CVIII, broken line in Fig 2) Thus, after injection ... effects Different proteins and nucleic acids gave rise to the different dose dependencies in Fig For instance, in competing with mRNA for the binding of regulatory proteins in the cytoplasm, PMLA...
... or post-theoretical training, nonstandardized in- service and continuing education programs across regions, and provincial differentials in technical support and dissemination systems Overall, ... thalidomide, smoking, and alcohol and drug abuse The long-term effects of such insults ultimately depend on a range of interrelated factors, including maternal health status and the timing of the insult ... development and high susceptibility to illness (Martorell, 1999) In summary, strengthening clinical case management of common infectious illnesses among children in low- and middle-income countries...
... rehydrated sections were stained with hematoxylin and eosin Immunohistochemical staining for antibacterial protein was performed using the avidin–biotin–peroxidase complex method using a Histofine SAB-PO ... markers are indicated inducing protein of Scomber japonicus (NCBI accession no CAC00499) A domain search showed that the gene detected in the gill of P stellatus contained a dinucleotide-binding motif ... derived amino acid sequence are shown The N-terminal and internal peptide sequences of antibacterial protein detected by amino acid sequencing analysis are indicated by boxes The predicted dinucleotide-binding...
... families and ourselves, it consists in creating capital; that is, earning as much as we can and, in any event, even if our earnings are fixed, managing the income thriftily, and carrying forward ... something in selfprotection, for the level of prices may be in some measure affected by his policy in handling his earnings A period of high wages is accompanied by and is in some sense an incident ... extraordinary earnings is one who is adding to the abnormal demand for such things as phonographs, jewelry, spirits, and tobacco And this helps to explain the tremendous market for luxuries during wartime...
... of lambs castrating and docking tagging or clatting Summer management and food washing shearing smearing and salving weaning drafting stall feeding—management on the prairies Diseases diarrhœa ... general management the poultry-house varieties diseases Turkey, the breeding and management Peacock, the Goose, the—varieties— breeding feeding and food Ducks—feeding—varieties breeding and rearing ... Ayrshire management of calves breeding breaking steers management of oxen fattening and stall-feeding Diseases hoven 26 26 27 29 30 35 38 39 41 42 42 45 41, 50 50 choking inflammation of stomach mange...
... developed and humus organomineral horizon This environment can be simulated during outplanting by mixing humification and organomineral horizons at a 3:1 volume ratio in a planting pit 40 × 40 cm in ... Damage derplantings increases with a decreasing content of these nutrients in needles An opposite correlation was determined for aluminium: with an increasing proportion of this element in nutrition ... služba: 153 (in Czech) Hŧ J., K P (2003) Modelling long-term changes in stream water and soil chemistry in catchments with contrasting vulnerability to acidification (Lysina and Pluhuv Bor,...
... sitka spruce clones and one cell line (574F) was maintained in sitka spruce embryo initiation medium [9] 2.1.3 Oak (Quercus robur L.) A single embryogenic cell line of oak was initiated from an ... on 200 µm nylon mesh and resuspension 200 mg samples of cells were analysed in triplicate The free proline concentration in the cells was determined using a modified ninhydrin method [3] 2.5 Statistical ... sitka spruce and oak is shown in Table I With no proline addition, intracellular proline levels are correspondingly low When proline is added, intracellular proline levels recorded correspond...
... International Science Foundation for supporting travel costs and to J Bohin and E Dreyer for help in organizing his participation in the meeting in Nancy, France the floodplain stand presenting ... the relationship between increment loss and index of defoliation was closer in suppressed and intermediate trees than in dominant and codominant ones; iii) latewood increment was more closely ... permanent plots were located in four main stand types: i) an upland stand, site index I-II, aged between 55 and 85 years, dominated by late oaks, and prone to water deficits; ii) a floodplain...
... to increasing PFD are ,) m shown in figure As expected, ΔF/F , m was high at low irradiance, and decreased steadily with increasing PFD in both controls and water-stressed seedlings Final ... Epron and Dreyer, 1990) In our case, high light favored an alteration in PS II reaction centers, as the reduction in m /F v F resulted from both a decrease in F m and an increase in F (Demmig and ... during rapid dehydration, remained constant until very high leaf-water deficits (D ≈ 0.75) (Epron and Dreyer, 1992) In our case, on potted seedlings drying out in a climate chamber, the decline...