167 changing permissions with terminal

... 9/05 • 101469 Building Entrance Terminals Building Entrance Terminals Enclosed, 5-Pin Protection with KRONE ® Series 2 Blocks 2 www.adc.com • +1-952-938-8080 • ... (-40 ˚ to 176 ˚ F) Indoor -20 ˚ to 70 ˚ C (-4 ˚ to 158 ˚ F) Building Entrance Terminals Enclosed, 5-Pin Protection with KRONE ® Series 2 Blocks Features: • 5-pin protection devices safeguard ... and equipment from voltage surges and spikes • Lockable metal enclosure with Telco override cover and a splicing chamber with the choice of either 710 or MS 2 ® splice connectors • 5-pin protectors...

Ngày tải lên: 21/12/2013, 07:17

Ngày tải lên: 13/02/2014, 12:20

... hSalvador interact via their C -terminal coiled-coil domains. (A–D) WT, C-terminally or N-terminally truncated mutants of flag-Sav cDNA were cotransfected with or without WT or C-terminally truncated mutants ... indica- ted with arrows while the position of flag- Sav is marked with arrowheads. (B) The position of CrmA-DQMD (lane 6) is marked with an asterisk (*). Each experiment was performed at least twice with ... homo-multimerize independently of its C -terminal coiled-coil domain. (A,B) HA-tagged Sav cDNA was co- transfected with either WT or C-terminally truncated mutants of flag-Sav cDNA or with HA-PPIA or flag-CrmA-DQMD...

Ngày tải lên: 19/02/2014, 06:20

... immunoprecipitated with antibody MAC 394 and analyzed by Western blots probed for the C -terminal tail of TAP1 with antiserum D90 (TAPwt and 1–2V1) or the C- terminal tail of TAP2 with antiserum 116/5 ... X-100 and incubated with different nucleotide agaroses. Bound proteins were eluted with SDS-sample buffer and analyzed in Western blots probed for the C -terminal tail of TAP1 with antiserum D90 ... combination with TAP2DV were lysed with 1% Triton X-100. Cell extracts were separated by SDS/PAGE and blotted onto nitrocellulose membranes. Western blots were probed with antiserum D90 (C-terminal...

Ngày tải lên: 20/02/2014, 02:21

... 5891 C -terminal domain of Eos, CtBP can also bind sites within the N -terminal domain of Eos. TRPS1 interacts with CtBP The TRPS1 protein is a multi-(zinc finger) protein that contains two C -terminal ... protein with six zinc fingers that comply with the Kru ¨ ppel C2-H2 consensus. The N -terminal fingers are involved in sequence-specific DNA binding [4], while the two zinc fingers that form the C -terminal ... motif in Eos supports only weak binding of CtBP, we show that additional regions within the N -terminal and C -terminal regions of Eos also contact CtBP, and, taken together, the results of GST-pulldown,...

Ngày tải lên: 21/02/2014, 01:21

... of trypsinogen 4 with a C -terminal GFP fusion protein; GFP-p72 M T4, p72 form of trypsinogen 4 with an N -terminal GFP fusion protein; p28 L T4-GFP, p28 form of trypsinogen 4 with a deleted ATG()44) ... digestion; N -terminal sequencing revealed the presence of both trypsin 4 with the N-ter- minal isoleucine and intact Isoform B starting with leucine (Fig. 1D). Searching for a protease with potential processing ... analyzed by N -terminal amino acid sequencing (Fig. 2C,D). Western blots, together with the N -terminal amino acid sequences of trypsino- gens isolated from 6 · 10 6 cells transfected with the p72 M T4...

Ngày tải lên: 07/03/2014, 10:20

... http://omics.pnl.gov/software/Decon2LS. php). The resulting csv files were further analyzed with viper [40]. A list with a delta m ⁄ z of 4.0247 corresponding to labeled ion pairs with a maximum mass tolerance of 10 p.p.m. was generated. ... suberate] with a 11.4 A ˚ spacer arm (Pierce, Waltham, MA, USA). Both cross-linkers react with the e-amino group of lysine residues and a-amino group from protein N-termini and, to a lesser extent, with ... 5112–5123 ª 2010 The Authors Journal compilation ª 2010 FEBS 5119 A region within the C -terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross-linkers and...

Ngày tải lên: 15/03/2014, 00:20

... were separated on a nonreducing SDS/PAGE with (+) or without (–) 20 m M dithiothreitol in the sample buffer. Detection of protein bands was carried out with a His 6 -antibody and chemiluminescence. Fig. ... cysteine residues in the N -terminal part of the proteins seems to interfere with but not abolish the activity. It has been reported in previous studies that the N-terminally truncated form of ... evidence for physical interactions of the N -terminal cysteine pair with the primary reaction centre. The situation in the C33S mutation appears to be different with respect to the intermediate formed....

Ngày tải lên: 17/03/2014, 10:20

... with uncleaved S1 and t-S1. The decrease in these parameters correlated well with the 50kDafi 45 kDa conversion (Fig. 1A). It has been sug- gested from this correlation that tryptic cleavage within both ... those S1 molecules, which were cleaved between Arg23 and Ile24 in the N -terminal region, but not within the 50 kDa segment. N -terminal cleavage dramatically decreases the thermal stability of S1 The ... mainly through electrostatic interaction of loop 2 with the negatively charged N -terminal part of actin [38–40]. Thus, the interaction of loop 2 with actin seems to be mainly responsible for actin-induced structural...

Ngày tải lên: 17/03/2014, 10:20

... Interaction of EW29Ch with sugars FEBS Journal 276 (2009) 2095–2105 ª 2009 The Authors Journal compilation ª 2009 FEBS 2105 NMR studies on the interaction of sugars with the C -terminal domain of ... barely interacts with the region of H1 and the O-methyl group. The crystal structure of EW29Ch with lactose showed that the O2, O3 and O4 atoms of the galac- tose residue of lactose formed hydrogen bonds with EW29Ch, ... interaction with lac- tose than the c sugar-binding site because of the num- ber of intermolecular hydrogen bonds and of residues interacting with lactose. Our results agreed well with those from...

Ngày tải lên: 23/03/2014, 04:21

... fitted with a · 60 oil objective, was used to collect the images. CFP was excited with a 458 nm laser and images were obtained using a bandpass filter from 475 to 525 nm. YFP was excited with a ... over glass fibre filters wetted with buffer C. The filters were washed thrice with buffer C. The radio- activity on these filters was counted using a b-counter calibrated with the 14 C isotope. Coimmunoprecipitation Cell ... ul- tracentrifugation and incubated with 50 lL of anti-flag M2 IgG agarose (rinsed with buffer B) at 4 °C for 1 h. The supernatant was removed and the antibody matrix was washed three times with buffer B containing...

Ngày tải lên: 23/03/2014, 07:20

... phenotype revealed that C -terminal truncation of SerRS abolished Pex21p binding. The interaction is lost upon deletion of the positively charged C -terminal fragment (mutant C13) within the extension. ... of a short C -terminal fragment of SerRS abolishes Pex21p binding without affecting catalytic activity A pull-down assay was used to verify that Pex21p spe- cifically recognizes the C -terminal appendix ... FEBS saturated with crude E. coli extract containing recom- binant Pex21p, which was immobilized on the resin by its N -terminal His-tag. Ni 2+ –nitrilotriacetic acid agarose precharged with Pex21p...

Ngày tải lên: 23/03/2014, 09:20

... assayed: FAPP, with a C -terminal phenylalanine residue (hydrolysed by carboxypeptidase A); FAAK, with a C -terminal lysine residue (hydrolysed by carboxypeptidase B) and FAEE, with a C -terminal gluta- mate ... a peptide with a C -terminal neutral, hydrophobic amino acid (Leu) was not hydrolysed, like the synthetic substrate FAPP (Phe C -terminal residue). Similarly, fibrinopeptide B, with a C -terminal ... complex with carboxypeptidase(s) in vivo. In contrast to the bands eluted at pH 12, the polypeptides eluted by 6 M guanidine hydrochloride had N -terminal sequences with similarity to, or identity with...

Ngày tải lên: 23/03/2014, 12:20

... together with 3 lg of either pCMV-Tag2 or pCMV-Tag2ÆCHD3. pCMV-Tag2ÆCHD3 expresses various N -terminal fragments of CHD3 with the indicated N -terminal end-point (from position 1676 –1943). The C -terminal ... times, with a minimum of n ¼ 4 for each data point. Values that differ sig- nificantly from the level of inhibition observed in cotransfections with the C -terminal amino acids 1676 –2000 of CHD3 (1676 ) ... of the C -terminal fragment (amino acids 1676 – 2000) with larger fragments including amino acids 295–1717, 1005–2000 and 1327–2000. The most signifi- cant activity was observed with the C-terminal...

Ngày tải lên: 30/03/2014, 09:20

... Coimmunoprecipitation of GluR-A CTD with SAP97. HEK293 cells were transfected for expression of wild-type (‘wt’) GFP-GluR- A CTD, and mutated CTDs L907A and ‘XA908’ with (A) or without (B) N-terminally myc-tagged ... hydrogen bond with the His384 at the N -terminal end of aB. These results are in agreement with our previous mutagenesis study which indicated that the ability of point-mutated GluR-A C -terminal domains ... C -terminal residues expressed in transgenic mice did not form an immuno- precipitable complex with SAP97 unlike its wild-type counterpart [10]. In vitro binding of SAP97 PDZ1-3 to GluR-A C-terminal...

Ngày tải lên: 30/03/2014, 10:20

Ngày tải lên: 30/03/2014, 20:20

Ngày tải lên: 31/03/2014, 23:20

Ngày tải lên: 18/06/2014, 18:20

Ngày tải lên: 19/06/2014, 10:20

Ngày tải lên: 20/06/2014, 01:20