13rα2 regulation in pancreatic cancer cell lines

... fetal bovine serum HCT116 and GEO cell lines were both wild-type p53, and SW620 lines were mutant p53 No antibiotics were used in our experiments, and all cell lines were incubated in a humidified ... provide a possible model of drug interaction in GEO cells, which involves the complex interaction of proteins involved in cell cycle regulation To recapitulate, combining CDO with cytotoxic chemotherapy ... we assessed p21waf1 levels in GEO and HCT116 cell lines, and showed that combination therapy induced p21waf1 in the GEO cell line only (the cell line in which synergistic effects were observed)...

Ngày tải lên: 07/03/2014, 15:20

... GSK1070916 The cell line response data was compared to the mutation patterns in the cell lines, gene expression patterns and the karyotypes of the cell lines High chromosome number in the cell lines was ... lymphoma cell lines, of the cell lines were resistant to GSK1070916 and contained low chromosome number in the primary population of cells However, when in reviewing the ploidy content in the cell ... number in T-ALL cell lines WT Mutant NOTCH1 Total Diploid (~2n) High Modality (>3n) 12 14 Total 10 14 23 proliferating nature of the established cell lines in tissue culture Since cancer cell death...

Ngày tải lên: 18/06/2014, 22:20

... GSK1070916 The cell line response data was compared to the mutation patterns in the cell lines, gene expression patterns and the karyotypes of the cell lines High chromosome number in the cell lines was ... lymphoma cell lines, of the cell lines were resistant to GSK1070916 and contained low chromosome number in the primary population of cells However, when in reviewing the ploidy content in the cell ... number in T-ALL cell lines WT Mutant NOTCH1 Total Diploid (~2n) High Modality (>3n) 12 14 Total 10 14 23 proliferating nature of the established cell lines in tissue culture Since cancer cell death...

Ngày tải lên: 20/06/2014, 04:20

... observed for the combinations of FWGE and 5-FU (6 out of cell lines) and to a lesser extend for irinotecan and oxaliplatin (2 out of cell lines) Drug interaction for the remaining cell lines was additive ... induced by FWGE in a panel of cell lines In order to distinguish the mode of cell death induced by FWGE we treated a representative panel of human cancer cell lines with an IC90 of FWGE for 48 ... irinotecan or oxaliplatin was assessed in a panel of colon cancer cell lines The mode of drug interaction was analyzed by the method of Drewinko and the data summarized in table Overall, mainly...

Ngày tải lên: 10/08/2014, 10:21

... 9(MMP-9), cyclinD1, and on cancer cells invasion, proliferation and cell cycle cell in two cell lines were investigated Page of 11 Construction of shRNA expression vector for MTA1 According to principle ... human breast cancer cell lines MDA-MB231, and protein levels of MMP-9 and CyclinD1 were down -regulation (P < 0.05) However, in ER alpha-positive breast cancer cells MCF-7, protein expression ... group in MCF-7 cells respectively As shown in the Figure, ER alpha protein expression was recovered positive in ERa-negative breast cancer cell lines MDA-MB-231, MMP-9 and CyclinD1 protein levels...

Ngày tải lên: 10/08/2014, 10:21

... effective in synchronizing DHDK12 cells in S phase than ara-C or aphidicolin Consequently, the efficacy of MTX in synchronizing cells in S phase was then tested in the HT29 cell line In HT29 cell line, ... France) cell lines Cell lines were cultured in DMEM medium containing 10% calf serum/penicillin (50 units/ ml)/streptomycin (50 μg/ml) at 37°C in 5% CO We used retroviral vectors carrying Escherichia-coli ... colon cancer cell lines used in this study, further investigations in more colon cancer cell lines are needed to draw definitive conclusion on the improvement of retroviral gene transfer after cell...

Ngày tải lên: 10/08/2014, 10:21

... an important role in determining radiosensitivity ated in MCF-7 cell lines This might be the reason why MCF-7 cell lines are more susceptible to HDAC inhibitor than RKO cell lines Histone deacetylation ... increased radiosensitivy in prostate cancer cell lines In this experiment, RKO cell lines showed a 56% survival rate with radiation alone, while with SB, 47% survived In MCF-7 cell lines, radiation alone ... radiosensitivity in MCF-7 and RKO cell lines In RKO cell lines, which are in a relatively hypermethylated state, the effect of 5-aza-DC was similar to that of SB; in MCF-7 cell lines, the effect...

Ngày tải lên: 09/08/2014, 04:21

... binding to nucleolin (black arrow) The competition experiments in UV cross-linking assays with the GT-recognizing proteins confirmed the results obtained with the total nuclear-enriched proteins: ... taking into account the different binding ability vs the same cytotoxicity Although a different intracellular localization could explain differences in antiproliferative effect, the protein binding ... oligodeoxynucleotides in human cancer cells DNA Cell Biol 22, 685–696 15 Scaggiante B, Morassutti C, Dapas B, Tolazzi G, Ustulin F & Quadrifoglio F (1998) Human cancer cell lines growth inhibition by...

Ngày tải lên: 16/03/2014, 13:20

... included in Table S2 Cell lines and cultures Five breast carcinoma cell lines were obtained from the Cell Resource Center of Peking Union Medical College (Beijing, China) and maintained in our laboratory ... levels in breast cancer cell lines and surgical specimens It was interesting to find that downregulation of miR-22 expression occurs frequently, not only in ERa-positive human breast cancer cell lines, ... Frequent downregulation of miR-22 expression in ERa-positive breast cancer cell lines and tumor specimens (A) For breast cancer cell lines, the expression levels of mature miR-22 were determined by...

Ngày tải lên: 22/03/2014, 21:20

... expression in gynecological cancer cell lines Methods Cell culture All the cell lines used were described in Table [20-45] and obtained from Cell Lines Service (Eppelheim, Germany) Briefly, all cells ... MMPs known in humans so far by measuring mRNA and protein level in twelve gynecological cancer cell lines commonly used in experimental research We examined cell lines of endometrium carcinoma (Ishikawa, ... other MMPs in at least one of the cell lines For MMP-8, -12 and -21 only very weak mRNA expression could be observed in single cell lines Nine MMPs, which were present in most of the cell lines, were...

Ngày tải lên: 28/03/2014, 14:20

... Oxaliplatin (Eloxatin®) (Figure 1) are first-line anti -cancer drugs in a broad variety of malignancies, for instance: ovarian cancer, testicular cancer and non small cell lung cancer Cisplatin is inactive ... For instance, hepatocellular carcinoma cells with elevated thioredoxin levels are resistant to Cisplatin, but not to the antracyclin Doxorubicin [39] However, bladderand prostate cancer cell lines ... cisplatin-induced apoptosis in ovarian cancer cells: possible role of upregulation of insulin-like growth factor-1 by hCG Int J Cancer 1998, 76:571-8 75 Kalli KR, Conover CA: The insulin-like...

Ngày tải lên: 10/08/2014, 10:21

... colony-stimulating-factor; HNSCC, head and neck squamous cell carcinoma; IGF, insulin-like growth factor; IκB, inhibitor kappaB; IKK, inhibitor-kappaB kinase; IL, interleukin; IL-R, interleukin receptor; ... status and all 10 p53 cell lines used (wt+mt), which include the cell lines with wild-type p53-deficient status Similarly, 117 genes were overlapping between the group of cell lines with mutant p53 ... and indirect physical and functional interactions from peer-reviewed publications The relationships in the network include protein-protein interactions, protein binding to DNA or RNA, protein...

Ngày tải lên: 14/08/2014, 08:20

... first investigated in breast cancer cells MTT assay was used to examine the effect of TQ on cell viability Several breast cancer cell lines (MCF-7, MDA-MB-231 and BT-474) were exposed to increasing ... Hypoxia-inducible factors α HRP Horseradish peroxidase IL-2 Interleukin IL-10 Interleukin 10 JNK c-Jun N-terminal kinases MAPK Mitogen-activated protein kinase MAPKKK Mitogen-activated protein kinase ... reduction in death risk (Slamon et al., 2001) On the other hand, lapatinib can bind to the ATP-binding pocket of EGFR/HER2 protein kinase domain preventing self-phosphorylation, which in turn, resulting...

Ngày tải lên: 10/09/2015, 09:09

... transcription regulation in cancer cell lines 3.3.5.2 β-catenin/TCF4 regulate hTERT promoter in cancer cell lines 3.3.5.3 Characterization of the distal TCF4 binding site (TBE) in the human hTERT ... 3.3.3.4 Effect of knocking down of β-catenin on TA, hTERT mRNA and hTERT (949bp) in cancer cell lines 3.3.3.5 Effect of knocking down of β-catenin on telomere length in cancer cell lines 3.3.4 Effect ... expression of β-catenin on TA, hTERT mRNA and hTERT (949bp) in cancer cell lines 3.3.5 β-catenin regulate the hTERT promoter through intereaction with TCF4 3.3.5.1 TCF is involved in β-catenin dependent...

Ngày tải lên: 02/10/2015, 17:14

... consequently integrins in mammalian cells (Tu et al., 2003) 1.3.2 Integrin signaling pathway in cell growth control Integrins are cell surface receptors connecting cells to the surrounding ECM They ... as a new binding partner for integrinlinked kinase (ILK), which is also an adaptor within integrin-associated cytoskeletal complexes implicated in integrin-mediated signaling (Mackinnon et al., ... |logGI50| of different drugs in the cell lines FIGURE1-1 Sensitivity against different drugs in cell lines A) One-Step RT-PCR showing the expression status of mig-2 in the cell lines B) The absolute...

Ngày tải lên: 05/10/2015, 22:15

... principal component for the cell lines (PC1, PC2, and PC3), two of the three AD cells lines were close, whereas the third cell line was close in PC2 to the other two cell lines, but separate in ... genes in test lung cancer cell lines (black and gray) and four validation cell lines (white) was quantified by qPCR and compared to a normal control cell line (MRC-9) Watanabe et al Cancer Cell International ... downregulation in PLAU Two SQ cell lines showed upregulation in 11 genes (AMY2A, BEX1, CDH1, DUSP4, HMGA1, IGSF3, INADL, ISL1, MALL, RAB25, and SLCO4A1) and downregulation in PLAU Two LC cell lines showed...

Ngày tải lên: 15/02/2014, 04:20

... sensitizing target to gemcitabine in pancreatic cancer cells Validation of CHK1 as a sensitizing target to gemcitabine in pancreatic cancer cells MIA PaCa-2 and BxPC3 pancreatic cancer cells were ... results will need to be done in other pancreatic cancer cell lines lated that inhibition of CHK1 results in the release of cells from checkpoint arrest, allowing progression into mitosis with unreplicated ... these CHK1 inhibitors in combination with gemcitabine would be more effective in treating pancreatic cancer patients than gemcitabine alone Moreover, in vivo experiments demonstrating that inhibitors...

Ngày tải lên: 18/06/2014, 15:20

... primarily found in pancreatic islet cells and rather sporadically in epithelial cells of pancreatic ducts as CCL20 in pg/ml per mg total protein A 15000 * shown in Figure 3A and 3B In CP tissues ... in necrotic parenchyma and sporadically in some epithelial cells of pancreatic ducts, whereas in PA CCL20 staining was found in epithelial cells of the net-like structures of PA tissues and in ... and in pancreatic islet cells In the PCA tissues we observed CCL20 immunoreactivity in the cytoplasms of ductal epithelial cancer cells, in infiltrates of perineural sheaths and also in tumor-associated...

Ngày tải lên: 18/06/2014, 16:20

... processes including development and cancer [16] EMT in cancer leads to the loss of cell- cell adhesion and cell polarity as well as altered cell- extracellular matrix interactions, resulting in invasion ... adenocarcinoma cell lines (Bic-1 and Seg-1), human gastric adenocarcinoma cell lines (AGS, AZ521 and KATOIII), colorectal adenocarcinoma cell lines (Caco-2 and DLD1), a human cervix epithelioid carcinoma ... Although Barrett’s adenocarcinoma is the most rapidly increasing cancer in Western countries [2], esophageal squamous cell carcinoma (ESCC) is still dominant in East Asia, including Japan [3] ESCC is...

Ngày tải lên: 18/06/2014, 19:20

... able to provoke intracellular disruption in hepatic cell lines at different levels, but a mixture of medicinal plant extracts Dig1 can protect to some extent human cell lines against this pollutants ... not prevent GST inhibition by R that could lead to accumulation of toxic reactive compounds Since the use of cell lines allows longer experiments, and since cell lines may in some instances be less ... endocrine disruptors in cell lines [10] We know that xenobiotics have a main endpoint in the liver, which is the major detoxification organ Here, we investigated the mechanism of action of R in...

Ngày tải lên: 20/06/2014, 00:20