13 c nmr spectra of secondary reference compounds

Báo cáo khoa học: Characterization of oligosaccharides from the chondroitin/dermatan sulfates 1 H-NMR and 13 C-NMR studies of reduced trisaccharides and hexasaccharides doc

Ngày tải lên : 16/03/2014, 14:20

... CS060# and CS#60# Residue Carbon CS040# (p.p.m.) CS060# (p.p.m.) CS#60# (p.p.m.) DUA (C) C1 C2 C3 C4 C5 C6 C1 C2 C3 C4 C5 C6 CH3 C O C1 C2 C3 C4 C5 102.83 71.54 67.51 109.41 147.01 – 102.97 55.02 ... region of CS is a repeating disaccharide of glucuronic acid (GlcA) and N-acetylgalactosamine (GalNAc) [-4)GlcA(b1– 3)GalNAc(b1-]n, which may be O-sulfated on the C4 and ⁄ or C6 of GalNAc and C2 of ... Discussion Isolation of oligosaccharides After depolymerization by chondroitin ABC endolyase, oligosaccharides are isolated as disaccharides, trisaccharides, tetrasaccharides and, because of incomplete...

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Báo cáo Y học: Elucidation of the role of fructose 2,6-bisphosphate in the regulation of glucose fluxes in mice usingin vivo 13 C NMR measurements of hepatic carbohydrate metabolism docx

Ngày tải lên : 17/03/2014, 17:20

... of net glycogen synthesis, respectively 13Glyc1 and 13Glyc6 represent 13Clabeled glycogen C1 and C6 concentration, and 13Glc6P1 and 13Glc6P6 represent 1 3C- labeled glucose-6-phosphate C1 and C6 ... isotopic enrichment of Glc6P in terms of Vphos as: 13 Glc6P1 ðtÞ ¼ ½Glc6P d 13 Glyc1 13 Glyc1 ðtÞ ðtÞ þ Vphos Á ½Glyc dt ð4Þ Vnet þ Vphos 13 Glc6P6 ðtÞ ¼ ½Glc6P d 13 Glyc6 13 Glyc6 ... resonance of glycerol C1 , C3 at 62.5 p.p.m from the resonance of glycogen C6 allowed, for the ﬁrst time, the measurement of glycogen C6 changes in vivo, which reﬂects the indirect pathway 13 C NMR spectra...

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nmr spectra of polymers and polymer additives

Ngày tải lên : 01/06/2014, 10:11

... chemical shift 1 3C 1H J-coupling Homonuclear correlated 1H chemical shift spectroscopy (COSY) 1H chemical shift Heteronuclear correlated 1 3C spectroscopy (COSY) 1H Double-quantum exchange 1 3C ... exchange 1 3C chemical shift spectroscopy (inadequate) 1 3C chemical shift chemical shift chemical shift I.D— Practical Considerations for NMR of Polymers Because of the large size of a polymer chain ... allows complex chemical structures to be described even more fully by facilitating correlation of various spectral parameters The applicability of NMR spectroscopy extends even beyond chemical characterization,...

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An error analysis of using reference in written english by secondary school studend = phân tích lỗi của học sinh THPT tong việc sử dụng phép quy chiếu trong tiếng anh viết

Ngày tải lên : 18/12/2013, 10:08

... conjuction are cohesive devices belonging to the grammartical cohesion However, in this study, we only deal with the cohesive device - reference Because of the significance of reference in creating ... the process of using English language, the learners are drawn to approach the recognization and creation of coherence of the text Textual coherence can be acheived by cohesive devices such as: ... is cataphoric When discussing cataphoric personal reference, Quirk et al (1985) say that cataphoric personal reference occurs less frequently, and under limited conditions Where it does occur,...

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Tài liệu BIOCHEMICAL TARGETS OF PLANT BIOACTIVE COMPOUNDS A pharmacological reference guide to sites of action and biological effects doc

Ngày tải lên : 17/02/2014, 05:20

... summarized below: + CH3CO-S-COA (C2 ) CH3COCH2CO-S-COA (C, ) -0 0C- CH2 -C( CH3,0H)-CH2-CO-S-COA (HMGCOA)(Cfj) -OOC-CH2 -C( CH:j,0H)-CH2-CH20H (mevalorlate) (Cfj) [via HMGCoA reductase NADPH] 3-phospho-5-pyrophosphomevalonate ... (Phe I C5 0L) Major simple pherlylpropanoids include cinnamic acid (Phe-CH=CH-COOH), p-coumaric acid (p-hydroxycinnamic acid), o-coumaric acid (0-hydroxycinnamicacid), caffeic Plant defensiue compounds ... their aglycones (deglycosylated entities) a-chaconidine (C6 I C6 I C6 I C5 I C4 N# I C5 N#), solanidine (C6 I C6 I C6 I C5 I C4 N# I C5 N#) and solasodine (C6 I C6 I C6 I C5 I C4 0 .C5 N), respectively...

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Báo cáo khoa học: NMR solution structure and function of the C-terminal domain of eukaryotic class 1 polypeptide chain release factor pdf

Ngày tải lên : 06/03/2014, 11:20

... polypeptide chain factors, eukaryotic class polypeptide chain release factor (eRF1) and eukaryotic class polypeptide chain release factor (eRF3) The major functions of eRF1 include recognition of each of ... experiment correlating side-chain protons via 1 3C- 1 3C correlations (HCCH)-TOCSY, measured at 313 K Additional side chain assignments and NOE distance restraints were extracted from the 1H,1 3C- NOESY ... prevent oxidation of the free Cys residues Cys302 and Cys335 Shigemi microcell NMR tubes, containing 330–380 lL, were used in the recording of the NMR spectra Cloning and mutagenesis of human eRF1...

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Báo cáo khoa học: NMR investigations of subunit c of the ATP synthase from Propionigenium modestum in chloroform/methanol/water (4 : 4 : 1) pot

Ngày tải lên : 08/03/2014, 10:20

... overlap The 1 3C chemical shifts were obtained by 3D 1 3C- resolved HCCH-COSY, 3D 1 3C- resolved HCCH-TOCSY and 3D 13 C- resolved [1H,1H]-NOESY experiments However, chemical shift dispersion of the methyl ... usage of different reference standards Secondary structure and global fold As indicated by helix-characteristic NOE connectivities [35], subunit c in chloroform/methanol/water (4 : : 1) consists of ... Alignment of c subunits from E coli and P modestum and location of the a helical secondary structures in diﬀerent subunit c preparations (a) Amino-acid sequence of E coli subunit c and location of a...

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Báo cáo khóa học: C-, 15N- and 31P-NMR studies of oxidized and reduced low molecular mass thioredoxin reductase and some mutant proteins docx

Ngày tải lên : 16/03/2014, 16:20

... mutant TrxR C1 38S mutant + PMA C( 2) C( 4) C( 4a) C( 5a) C( 6) C( 7) C( 7a) C( 8) C( 8a) C( 9) C( 9a) C( 10a) C( 10a) C( 10b) C( 10 c) C( 10d) C( 10e) N(1) N(3) N(5) N(10) 159.8 163.7 136 .2 136 .4 131 .8 140.4 ... determining 1 3C chemical shifts than is the case for 1H shifts Steric and stereochemical effects, however, can considerably inuence the 1 3C chemical shifts [29] Oxidized enzymes The 1 3C- NMR spectrum ... 109:5 dCi;sp ị dCi;sp ị 1ị where, Ci is C( 9a) or C( 10a) for the calculation of the endocyclic angle of N(5), d(Ci,sp3) and d(Ci,sp2) are the corresponding limit chemical shifts of carbon atom Ci...

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Báo cáo khoa học: NMR studies on the interaction of sugars with the C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris pot

Ngày tải lên : 23/03/2014, 04:21

... each of the two sugar-binding sites (a and c) of EW29Ch had a distinct chemical exchange on the chemical-shift timescale Site-speci c dissociation constants determined by NMR The site-speci c ... STD–TOCSY and STD–[1H,1 3C] HSQC spectra for the mixture of lactose (5 mM) and EW29Ch (50 lM) (A) Reference TOCSY spectrum of the mixture of lactose and EW29Ch at a ratio of 100 : (B) The STD–TOCSY ... were recorded consecutively with increasing concentrations of each sugar For the progressive chemical-shift changes of EW29Ch under conditions of fast exchange on the chemical-shift timescale,...

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Báo cáo khoa học: Structural function of C-terminal amidation of endomorphin Conformational comparison ofl-selective endomorphin-2 with its C-terminal free acid, studied by 1 H-NMR spectroscopy, molecular calculation, and X-ray crystallography pot

Ngày tải lên : 30/03/2014, 20:20

... dependence of their NMR spectra Characteristically, the NMR spectra of EM2 in water of pH 2.7 and DPC micelles of pH 3.5 were the same as those in solutions of pH 5.2 This was in contrast with the case ... the conformers of EM2 in water and DPC micelles were hardly inﬂuenced by the variation in pH, because the NMR spectra of EM2 in a solution of acidic pH were identical to those in solution of pH ... This is in contrast with the EM2OH conformers, whose NMR spectra differed considerably depending on pH (discussed later) A characteristic feature of most conformers of EM2 in DPC micelles was...

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Báo cáo Y học: NMR structure of the HIV-1 regulatory protein Vpr in H2O/triﬂuoroethanol Comparison with the Vpr N-terminal (1–51) and C-terminal (52–96) domains pot

Ngày tải lên : 31/03/2014, 23:20

... respectively The occurrence of typical a helix encompassing these two RESULTS Circular dichroism CD spectra of the protein in 100% H2O (Fig 1) are characteristic of ordered conformations as illustrated ... Licha, K., Kopp, J., de Moronha, C. M .C. , Greene, W .C. , Wray, V & Schubert, U (2000) Functional and structural characterization of synthetic HIV-1 Vpr that transduces cells, localizes to the nucleus, ... of the resonance intensity and I8 corresponds to the steady state value Structure calculation Calculations were performed with the DISCOVER/NMRCHIsoftware package from MSI with the Amber forceﬁeld...

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Wide Spectra of Quality Control Part 13 pptx

Ngày tải lên : 19/06/2014, 08:20

... affective tests (2) evaluate the personal response (preference or acceptance) to a new product, or a single characteristic of a product The affective tests involve the acceptance methods, the preference ... G5 was characterized by citric attribute (probably because the 370 Wide Spectra of Quality Control species added were more citric as cardamom, coriander or citrus peel) while G6 was characterized ... order of preference The acceptance test is used by to rank the products in a scale of acceptability while attribute diagnostics consists in rank the principal attributes that determine the acceptance...

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Feeding ecology of the sandbar shark in south carolina estuaries revealed through δ13 c and δ15 n stable isotope analysis

Ngày tải lên : 04/09/2015, 17:08

... (i.e., δ1 3C × year : sex) were signiﬁcant, interaction terms were omitted from this table Comparison df Mean square F P δ1 3C × year δ1 3C × sex δ1 3C × estuary δ1 3C × month δ1 3C × age-class 4 9.85 ... 0.58 0.298 0.048 169 FEEDING ECOLOGY OF THE SANDBAR SHARK IN SOUTH CAROLINA TABLE A.2 Continued Comparison δ1 3C × estuary δ1 3C × month δ1 3C × year δ1 3C × sex δ1 3C × age-class (age-0: N = 58 versus ... E Shackley 1996 The comparative feeding ecology of six species of shark and four species of ray (Elasmobranchii) in the north-east Atlantic Journal of the Marine Biological Association of the...

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c# 3.0 the complete reference (3rd edition)

Ngày tải lên : 06/08/2013, 17:29

... architect of the Security Development Lifecycle (SDL), a process for improving the security of Microsoft’s software Howard speaks regularly on the topic of securing code for Microsoft and at conferences ... author of numerous bestsellers, including Java: The Complete Reference, C+ +: The Complete Reference, C: The Complete Reference, and C# : A Beginner’s Guide Although interested in all facets of computing, ... type of car Chapter 2: An Overview of C# Inheritance Inheritance is the process by which one object can acquire the properties of another object This is important because it supports the concept...

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Estimation of emissions of nonmethane organic compounds from a closed landfill site using a landfill gas emission model

Ngày tải lên : 05/09/2013, 16:11

... indicates that the uncertainty in the concentration of NMOC, annual waste acceptance rate, and the age of the landfill have significant effect on the expected mass emission outcome of NMOC When considered ... the fact that volatile organic compounds (VOCs), a major group of NMOC are recognised to be highly carcinogenic even in low concentration [16]; are the main reasons nonmethane organic compound ... time since closure (years) Note that for active landfills, c = 0, CNMOC = the concentration of NMOC (ppm by volume as hexane) The constant is a conversion factor to render the outcome of the emission...

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Molecular Biology of Secondary Metabolism - Case Study for Glycyrrhiza Plants

Ngày tải lên : 25/10/2013, 05:20

... Metabolic pathway of glycyrrhetinic acid in cultured licorice cells licorice cells Although the glucuronosyltransferase for glycyrrhetinic acid was not detected in the cultured cells, the glucuronosyltransferase ... products glycyrrhetinic acid O COOH O O C COOH O C O O O CH2OH O O CH2OH O O CH2OH O O HO CH2OH O O OH OH HO HO HO OH OH OH O OH HO HO OH OH O COOH COOH COOH O C O O O O CH2OH O O COOH O O HO CH2OH ... licorice-saponin B2 HO COOH O O OH HO licorice-saponin C2 OH HO HO OH OH OH O C COOH HOOC O O O COOH O O COOH O O OH HO COOH O O OH OH licorice-saponin E2 HO COOH O O O COOH O O CH2OH licorice-saponin...

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The Natural Functions of Secondary Metabolites

Ngày tải lên : 26/10/2013, 02:20

... synthetic compounds The above successes came about in two ways: (i) broad screening of known compounds which had failed as useful antibiotics; and (ii) screening of unknown compounds in process ... important in colonization of the human intestinal tract by Escherichia coli early in life Cyanobacteria cause human and animal disease by producing cyclic heptapeptides (microcystins by Microcystis) ... sclerotia which act against the corn earworm insect, Helicoverpazea Similarly, sclerotia of Claviceps spp contain ergot alkaloids in high concentration which are considered to protect the sclerotia...

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Tài liệu Glossary of Volatile Organic Compounds ppt

Ngày tải lên : 14/02/2014, 03:20

... severe cases, coma and even death can occur In laboratory animals, carbon tetrachloride given by mouth can cause more liver tumors We not know if breathing carbon tetrachloride causes cancer in ... periods can cause headaches, lack of muscle coordination, dizziness, confusion, and changes in balance Acute exposures also irritate the skin, eyes, nose, and throat, and cause difficulty breathing, ... levels of benzene in the air can cause a particular type of leukemia called acute myelocytic leukemia Measuring exposure In a large U.S study, 95% of the participants had a benzene concentration...

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Tài liệu Báo cáo khoa học: C-Terminal extension of a plant cysteine protease modulates proteolytic activity through a partial inhibitory mechanism doc

Ngày tải lên : 14/02/2014, 14:20

... C rproErv -C CT 29 kDa 24 kDa rmErv -C CT 20 kDa M 45 30 25 20 15 10 C C B rproErv -C+ CT rproErv -C CT rmErv -C+ CT rmErv -C CT 2 Fig (A) Time course of activation to the mature form (I) rproErv -C+ CT ... determined kcat (s)1)a rproErv -C+ CT rproErv-CDCT Latex Erv -C [17] Km (lM)a kcat ⁄ Km (s)1ÆmM)1) Speci c activity at 37 °Cb (UÆmg)1) Speci c activity at 65 °Cb (UÆmg)1) IC50 against E-64 (nM )c 0.0170 ... mature catalytic domain is ˚ 1037 A2, which is 56% of the total surface area of the CT-extension The Leu side chain at the position 23 of the CT-extension occupies speciﬁcity pocket S2 of the catalytic...

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Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx

Ngày tải lên : 16/02/2014, 09:20

... (forward primer, 5¢-GAC CAGCTTCATGTGGCCATCGTGCAGGTT-3¢; reverse primer, 5¢-CACGATGGCCACATGAAGCT-3¢) to PCRamplify the coding regions of these domains in the panC gene from E coli genomic DNA [44] The ... were enriched in 2H, 1 3C and 15 N Figure shows the assigned 1H–15N correlation spectrum of nPS Sequence-speci c resonance assignment for the backbone 1HN, 13Ca, 13Cb, 1 3C and 15N nuclei of the ... between protein backbone conformation and Ca and Cb 1 3C nuclear magnetic resonance chemical shifts J Am Chem Soc 113, 5490–5492 26 Wishart DS & Sykes BD (1994) The 1 3C chemical-shift index: a...

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