11  moving cells and sections in table views

... stain technique Protein concentration was determined by a dye binding assay (Bio-Rad) using bovine serum albumin as standard [30] Enzyme kinetics Standard ADH activity was determined by measuring ... all-trans-retinol and all-trans-retinal, and with the cis-isomers of retinol and retinal (7-cis-, 9-cis-, 11-cis- and 13-cis-), were determined for human and mouse ADH1 and ADH4 enzymes (Tables and 3, ... rodent ADH1 and ADH4 show a wide specificity toward retinoids, using efficiently the all-trans and most of the cis isomers of retinol and retinal, including the 11-cis-retinoids involved in photosensitivity...

Ngày tải lên: 23/03/2014, 12:20

... Taipa, Macao, China 2School of Chinese Medicine, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China Competing interests The authors declare that they have no competing interests References ... anthraquinone, naphthopyranones, protocatechuic acid and c-tocopheryl acetate being the bioactive constituents [10] Flavonoids such as patuletin hispidulin, quercetin, quercetagetin and quercetagetin ... Anti-tyrosine hydroxylase (TH) whole mount immunostaining Zebrafish were fixed in 4% paraformaldehyde in phosphate buffered saline (PBS) for five hours, rinsed and stored at -20°C in 100% EtOH...

Ngày tải lên: 13/08/2014, 14:20

... gastro-intestinal tract bleeding during aspirin therapy Clopidogrel was used in patients intolerant to aspirin therapy Other commonly used drugs included statins, angiotensin converting enzyme inhibitors ... symptoms) to (indicating death)) Imaging studies and laboratory investigations In addition to full clinical assessment, other examinations performed also included chest X-ray film, routine brain computed ... HKY participated in refinement of experiment protocol and coordination and helped in drafting the manuscript All authors have read and approved the final manuscript Competing interests The authors...

Ngày tải lên: 14/08/2014, 07:21

... gastrointestinal neoplasm (23, 3.0%), nonoperative gastrointestinal bleeding (21, 3.2%), trauma (50, 6.6%), and operative gastrointestinal bleeding (15, 2.0%) The number of transfusions and the ... Service, and in- kind support from the Australian and New Zealand Intensive Care Research Centre The present study is a collaboration of the Australian and New Zealand Intensive Care Society Clinical ... study in Australian and New Zealand ICUs All sites that were members of the Australian and New Zealand Intensive Care Society (ANZICS) Clinical Trials Group were invited to participate, and 47...

Ngày tải lên: 14/08/2014, 08:21

... certain inflammatory reactions J Clin Invest 101(4): 746-754 Ralph P, Nakoinz I and Rennick D 1988 Role of interleukin 2, interleukin 4, and alpha, beta, and gamma interferon in stimulating macrophage ... DJ and Boonstra A 2002 In vitro generation of interleukin 10producing regulatory CD4+ T Cells is induced by immunosuppressive drugs and inhibited by T helper type (Th1)- and Th2-inducing cytokines ... 1987 Diphtheria toxin receptor binding domain substitution with interleukin2: genetic construction and properties of a diphtheria toxin-related interleukin-2 fusion protein Protein Eng.1: 493 Williamson...

Ngày tải lên: 16/09/2015, 17:13

... recruitment of inflammatory cells into the liver and skin, but not into the heart, in acute GVHD The predominantly expressed chemokines MIP1α and Mig in the spleen, liver and skin recruit Th1 cells, ... organs involved in acute GVHD and the roles played by chemokines in directing the infiltration of donor T cells into the host target organs The inflammatory reaction in acute GVHD begins with ... 1987) and expressing the recombinant fusion protein in E coli This genetic engineering procedure is cheaper and has the potential of optimizing the construct with respect to efficacy and minimizing...

Ngày tải lên: 16/09/2015, 17:13

... chemokine expression pattern is a factor determining the preferential organ involvement in acute GVHD In this study, we examined T cell infiltration and chemokine expression in target and non-target ... of T cells infiltrating in the liver (a) and the skin (b), but not in the heart (c) Spleen Liver Intestine Heart Kidney Skin Day - Day3 ++ + - Day7 +++ ++ +/+/- Day21 +++ +++ ++ ++ Table Table ... cell infiltration occurred in the target organs, liver, skin and intestines All these target 97 _ Results organs had intense infiltrating T cells (Fig 16), indicating...

Ngày tải lên: 16/09/2015, 17:13

... FS and SS) (Fig 1a), and cells that retained the original CFSE fluorescent intensity (non-proliferating cells) and cells that showed highly reduced CFSE fluorescent intensity (early proliferating ... Heart, MLN and PLN cells were obtained by mincing the tissues and the tissue debris filtered using a 41 µm mesh filter (Sefar, Switzerland) All cells were washed twice prior to staining with mAbs ... and LN cells were obtained by mincing the LNs and the tissue debris filtered using a 41 µm sterile mesh filter (Sefar AG, Switzerland) After washing twice with PBS, various numbers of these cells...

Ngày tải lên: 16/09/2015, 17:13

... functional domains – a cell binding domain, a translocation domain facilitating transport to the cytosol, and/ or a catalytic domain inhibiting protein synthesis Toxin engineering for therapy ... genetically fusing the toxin translocation and/ or catalytic domains to new cell-selective ligands or modifying amino acid residues in the cell binding domain and conjugating the altered toxin to the ... express single-chain ribosome inactivating proteins including pokeweed antiviral protein from Phytolacca americana and saporin from Saponaria officinalis All of these proteins contain at least...

Ngày tải lên: 16/09/2015, 17:13

... prewriting, drafting, revising and editing 2.1.3 The Stages of Teaching Writing Based on the process of writing, teaching writing includes three stages, namely pre-writing, while-writing, and post-writing ... Classifying the data into intended groups Doing statistical calculation Describing the collected data presented in tables and figures 27 Generalizing and giving comments and evaluations 3.3 Findings ... fluency in the first drafts, using paraphrases and synonyms, soliciting peer and instructor feedback, and using feedback for revising and editing Brown’s list of micro skills for writing includes...

Ngày tải lên: 20/12/2013, 18:33

... headspace method, the H2S gas in the headspace is decomposed according to heating temperature and time, resulting in variation in data obtained However, H2S is relatively stable in the acetone solution ... from urine in of the patients In a case in which subjects were exposed to H2S gas during working in a close position to an instrument for excluding acidic gas at an ammonia- manufacturing factory, ... hydrogen sulfide) in blood and urine 14) Tsuge K, Kataoka M, Seto Y (2000) Changes of sulfide and thiosulfate in blood and urine during storage in a refrigerator Jpn J Sci Tech Iden 4:83–90 (in Japanese...

Ngày tải lên: 26/01/2014, 18:20

... may occur during the initial h window of infection We infected RPE cells with K26GFP [27], and stained cells for F-actin, using phalloidin at 30 and h postinfection, and examined the cells under ... to fully understand HSV-1 infection of all target cells, including RPE cells, our study provides new starting points for understanding viral pathogenesis in the retina and advancing novel therapies ... entry into RPE cells V Tiwari et al Fig Role of nectin-1 during HSV-1 entry into RPE cells (A) Antibody against nectin-1 significantly inhibits HSV-1 entry into cultured RPE cells Cells (indicated)...

Ngày tải lên: 18/02/2014, 14:20

... washings in PBG, and a further six washings in NaCl ⁄ Pi, incubation with 2.5% (w ⁄ v) glutaraldehyde in NaCl ⁄ Pi, and further brief washes in destilled water, sections were stained by incubation ... localization in subconfluent cells was determined Figure 5A shows a Caveolin-1 staining pattern at the cell surface, confirming the known caveolar location of Caveolin-1 In addition, intracellularly distinguishable ... component In this study, the authors imply that P2X7 receptors in nonraft microdomains of submandibular gland cells are solely involved in ion channel activity, and that receptors present in lipid...

Ngày tải lên: 19/02/2014, 00:20

... GSM-900 and cell stress in skin models S Sanchez et al expression under stress conditions has been reported in a number of cell types, including skin cells The major HSPs expressed in the skin [5] ... expression and induce DNA damage in a fibroblastic cell line and human fibroblast primary cells, respectively [58,59] In our study, the effects of GSM-900 exposure on human skin cells were investigated ... expression in fibroblasts and hRE Results Human skin cells GSM-900 signal did not induce apoptosis or affect HSP expression in normal human epidermal keratinocytes As shown in Fig 1A, in normal...

Ngày tải lên: 07/03/2014, 11:20

... their ability to bind a number of heparin-binding proteins [28] In our search for allele-speci®c inhibitors based on singlenucleotide polymorphisms in target mRNA sequences using antisense PS-ODNs, ... ODN-mediated inhibition of gene expression in vivo This would be compatible with the ®nding that antisense ODNs can be very effective in inhibiting gene expression in the brain [44± 46] In both fetal and ... 10% of the input RNA remains uncut In both cases, the range from 0.5 to lL extract is not yet saturating, indicating a similar level of activity in both cells Perhaps we measure two distinct activities...

Ngày tải lên: 08/03/2014, 10:20

... H2O2-induced apoptosis in transfected C2C12 cells Furthermore, there was no direct interaction between HSP70 and Smac proteins, and the ATP-binding domain of HSP70, rather than the peptide-binding ... apoptosis is not attributable to a direct physical interaction between HSP70 and Smac HSP70 contains three functional regions: the ATPbinding domain, the peptide-binding domain, and the EEVD motif ... the ATP-binding domain of HSP70 is critical for sequestering AIF in the cytosol [29] In the present study, we demonstrated that the ATP-binding domain of HSP70 was indispensable for inhibition...

Ngày tải lên: 16/03/2014, 01:20

... pathways, the ERK1 ⁄ and c-Jun N-terminal kinase pathways, through AP-1 signaling [26] AP-1 family proteins are good candidates for mediating NGF-induced miR-221 ⁄ 222 expression in PC12 cells A previous ... function in different biological processes Our findings provide new insights into the MAPK signaling pathway 3274 Experimental procedures Cell culture and transfection PC12 cells were maintained in ... effectively induce miR221 and 222 in PC12 cells Finally we identified the BH3-only protein Bim, which is involved in NGF-dependent neuronal survival [17–19], as a potential target of miR-221 and 222...

Ngày tải lên: 16/03/2014, 01:20

... imaging of post-Golgi sorting and trafficking in live cells Nat Cell Biol 3, 140–149 17 Ait Slimane T & Hoekstra D (2002) Sphingolipid trafficking and protein sorting in epithelial cells FEBS Lett 529, ... Consequently, the internalization of these domains may well play an important role in signalling events GD3 and GM1 internalization and trafficking in MDCK cell lines were monitored using a specific ... GD3 in MDCK cells A B C Fig GD3 and GM1-CTx are internalized in MDCK cells by clathrin-independent endocytosis (A) GD3-expressing MDCK cells were transiently transfected to express the dominant-negative...

Ngày tải lên: 16/03/2014, 04:20

... the fifth painting and subjected to RNA extraction Replicate ear sections were fixed by formalin, embedded in paraffin and stained with hematoxylin and eosin to verify the progress of in ammation ... and WiDr cells and weakly expressed in KM20L2 cells COX-2 was detected only in WiDr cells The two terminal PGE2-biosynthetic enzymes, cPGES and mPGES, were expressed in all cell lines Following ... (WT) and two mutants of mIID in HEK293 cells, as assessed by RNA blotting (C) Membrane binding of the WT and two mutants of mIID After collecting the culture supernatants, the cells were incubated...

Ngày tải lên: 18/03/2014, 01:20

... indicators appear in the right-hand scroll area when the table is in motion The Indexed Table The indexed table builds on the plain table by adding an extra navigation aid in the form of an index ... -(BOOL)tableView:(UITableView *)tableView canMoveRowAtIndexPath:(NSIndexPath *)indexPath; - (void)tableView:(UITableView *)tableView moveRowAtIndexPath:(NSIndexPath *)fromIndexPath toIndexPath:(NSIndexPath ... react to user input These methods support selecting, editing, reordering, and deleting cells, in addition to configuring how the table view looks For www.it-ebooks.info CHAPTER 1: Table Views from...

Ngày tải lên: 23/03/2014, 04:20