1 department of microbiology national university of singapore singapore 117597
Engineering of an efficient and enantioselevtive biocatalyst for the preparation of chiral pharmaceutical intermediates
... transfer reducing equivalents to NAD(P)+ .10 9 -11 6 However, these methods generally suffer from catalyst or electrode fouling, or low productivity .11 1 ,11 2 ,11 4 ,11 6 ,11 7 Other enzymatic methods that have ... 10 8 4.4 Materials and Methods 11 0 Chapter 5 : Further Characterization of P450pyr and Related Mutants 11 5 5 .1 Introduction 11 5 5.2 Results 11 7 ... Rational Design 10 1. 4.2 Directed Evolution 11 1. 4.3 Screening and Selection 14 1. 5 Cofactor Regeneration 24 1. 5 .1 1. 6 NAD(P)H Regeneration...
Ngày tải lên: 12/09/2015, 10:22
Tài liệu Báo cáo khoa học: Medium-chain dehydrogenases/reductases (MDR) Family characterizations including genome comparisons and active site modelling pdf
... )0 .18 0 .16 0.26 0.55 0 .14 0 .14 0.26 0.26 0.03 )0 .11 )0 .11 )0.24 )0.68 )1. 28 0.59 0.77 )0.34 13 .2 16 .7 16 .2 15 .8 16 .4 16 .8 15 .8 16 .9 15 .9 14 .6 14 .6 12 .9 12 .9 13 .3 12 .5 12 .5 13 .2 9.7 12 .1 10.2 7.0 ... numbering of LB4D_HUMAN f Active site residues are at positions 15 67, 15 69, 15 70, 15 72, 15 73, 15 76, 15 86, 16 11, 16 12, 16 15, 16 45, 16 49, 17 66, 17 81, 17 90, 17 93 and 17 94 in the numbering of FAS_HUMAN ... LTDe AT_F2K13 11 0 AT_F2K13 14 0 AT_F2K13 15 0 AT_F2K13 12 0 AT_F2K13 13 0 AT_T17B22–23 AT_F28B23–3 AT_K18L3 10 0 AT_k19a23 10 AT_F24G16 11 0 AT_F5I14–32 HS_LB4D_HUMAN EC _14 20_b1449 CE_M106–3_short...
Ngày tải lên: 21/02/2014, 03:20
Báo cáo khoa học: The lactate dehydrogenases encoded by the ldh and ldhB genes in Lactococcus lactis exhibit distinct regulation and catalytic properties ) comparative modeling to probe the molecular basis pdf
... ± 5.4 0 .1 ± 0.05 0.2 ± 0 .1 0.3 ± 0.04 – ± 0 .1 1.3 ± 0.02 0.7 ± 0 .1 – 22 71 78 75 ± ± ± ± 0.4 0 .1 1 .1 1.8 ± 1. 3 – ± 0 .1 ± 1. 1 NADH in the range 0.03 1. 7 mM, 10 mM pyruvate, and mM Fru (1, 6)P2 b ... KPi 10 0 mM KPi No Fru (1, 6)P2 No KPi mM KPi 50 mM KPi 10 0 mM KPi a c LDH pH 6.0 pH 7.0 pH 6.0 pH 7.0 10 0 ± 6.2 11 0 ± 2.6 – 10 0 ± 2.6 88 ± 0.7 – 10 0 ± 0.2 10 0 ± 0.7 90 ± 0.6 10 0 ± 1. 6 99 ± 1. 1 71 ... Lactacte dehydrogenases of Lactococcus lactis 29 30 31 32 33 34 35 36 37 38 39 40 P Gaspar et al duction on glucose metabolism of Lactococcus lactis Microbiology 15 0, 11 03 11 11 Luesink EJ, van Herpen...
Ngày tải lên: 16/03/2014, 05:20
Báo cáo khoa học: High and complementary expression patterns of alcohol and aldehyde dehydrogenases in the gastrointestinal tract Implications for Parkinson’s disease docx
... Medical Research Support for construction of the B6.Adh1– ⁄ – congenic strain was provided by NIH grant AA 118 28 10 11 12 References 13 Farrer MJ (2006) Genetics of Parkinson disease: paradigm shifts ... kinetic FEBS Journal 274 (2007) 12 12 12 23 ª 2007 The Authors Journal compilation ª 2007 FEBS 12 21 Expression of ADH and ALDH in the rodent GI tract 17 18 19 20 21 22 23 24 25 26 27 28 29 30 M ... Aldh1 Probe Gene Species Sequence 5’- to 3’ Adh1 Adh1 -1 Adh1-2 Adh3 Adh3 Adh4 Adh4 -1 Adh4-2 Adh4-3 Aldh1 Random Adh1 (class I Adh) Adh1 (class I Adh) Adh1 (class I Adh) Adh3 (class III Adh) Adh3 (class...
Ngày tải lên: 16/03/2014, 11:20
Báo cáo khoa học: Prediction of coenzyme specificity in dehydrogenases ⁄ reductases A hidden Markov model-based method and its application on complete genomes doc
... dehydrogenases 11 80 Total proportion (%) Human Chimp Mouse Rat Fish Fly Worm Yeast Sum 26 71 17 62 13 68 16 67 23 79 11 57 11 75 13 492 10 5 5 3 2 18 15 12 10 17 14 12 12 12 8 10 17 10 11 13 10 11 5 8 11 82 ... 57 87 11 5 14 2 56 400 19 28 60 11 6 41 135 18 6 370 17 9 376 505 96 11 56 FEBS Journal 273 (2006) 11 77 11 84 ª 2006 The Authors Journal compilation ª 2006 FEBS 11 81 Prediction of coenzyme specificity ... size FAD NAD NADP Total 26 38 31 95 10 13 25 4793 4769 4774 4694 0.929 0.792 0.705 0.792 1. 000 0.999 0.999 0.999 48 21 48 21 48 21 48 21 FEBS Journal 273 (2006) 11 77 11 84 ª 2006 The Authors Journal...
Ngày tải lên: 23/03/2014, 10:21
Báo cáo khóa học: Acyl-CoA dehydrogenases and acyl-CoA oxidases Structural basis for mechanistic similarities and differences pot
... (19 97) Molecular cloning and expression of cDNA 16 17 18 19 20 21 22 23 24 25 26 27 28 29 encoding 3a,7a ,12 a-trihydroxy-5b-cholestanoyl-CoA oxidase from rabbit liver J Biol Chem 272, 18 4 81 18 489 ... homology of four enzymes of the acyl-CoA dehydrogenase family J Biol Chem 264, 16 3 21 16 3 31 13 Goodman, S.I., Kratz, L.E., DiGiulio, K.A., Biery, B.J., Goodman, K.E., Isaya, G & Frerman, F.E (19 95) ... New York 10 Thorpe, C., Matthews, R.G & Williams, C.H Jr (19 79) Acylcoenzyme A dehydrogenase from pig kidney Purification and properties Biochemistry 18 , 3 31 337 11 Wang, R & Thorpe, C (19 91) Reactivity...
Ngày tải lên: 23/03/2014, 12:20
Báo cáo khóa học: Genetic defects in fatty acid b-oxidation and acyl-CoA dehydrogenases Molecular pathogenesis and genotype–phenotype relationships ppt
... [55] 12 – 34 – – – – – – – – – – – – – – 20 1 – – – – – – – – – 1. 7 0.9 2.3 2.0 2.0 2.3 1. 4 1. 3 0.9 1. 2 10 18 20 20 16 12 10 25.8 16 .5 [ 81] 60.03 17 .8 5.3 54.0 45.5 38.0 45.5 13 15 q23 19 q13 4q32 ... out-offrame deletions/insertions, stop-codon introductions and Ó FEBS 2004 1 – – 12 13 11 0.6 21. 2 36.4 1. 0 0.8 1. 9 – – – – – – 1 2 – – – 13 – 20 47 15 13 – – – – – – – – – – – – – 13 – 19 41 – 13 ... 17 .8 5.3 54.0 45.5 38.0 45.5 13 15 q23 19 q13 4q32 5q33 3p 21 11q13 22q13 17 p 11 2p23 2p23 1p 31 4q22 12 q22 CAT SLC22A5 CACT SLC25A2 CPTI CPT1A CPTII CPT2 VLCAD ACADVL LCHAD HADHA LCKAT HADHB MCAD...
Ngày tải lên: 23/03/2014, 12:20
Báo cáo khoa học: Inhibition kinetics of catabolic dehydrogenases by elevated moieties of ATP and ADP – implication for a new regulation mechanism in Lactococcus lactis potx
... 0. 21 0. 41 0.22 1. 07 1. 32 1. 40 1. 28 1. 65 0.95 1. 04 0.96 1. 03 0.57 1. 17 0.86 1. 52 0 .10 0.23 0 .19 0 .16 0 .14 0 .19 0 .17 0.42 0 .13 0.74 1. 27 4.53 0.90 0.83 14 .6 3 .16 1. 80 36.7 12 0 2.58 ... 0 .16 0.24 0 .14 0 .15 0.05 0.02 0 .12 0.07 0. 01 0 .11 0.63 KM value for NADH (3.6 lM) taken from [26] result_at.php4?ecno =1. 1 .1. 1) b Goodness of t n2 0 .15 1. 25 0 .14 0.23 0.79 0. 21 0. 21 ... 2.55 1. 90 1. 56 1. 64 4. 61 1.45 3.43 2.03 0.96 0.27 KIU 0.54 0.20 1. 48 0. 51 0.34 0.20 0.78 0.36 0.26 0.26 n 4 .16 0.92 5.38 2.76 5. 21 5.69 1. 15 1. 75 0.94 0.94 4.02 1. 42 1. 55...
Ngày tải lên: 29/03/2014, 08:20
Báo cáo hóa học: " Genetic variation and recombination of RdRp and HSP 70h genes of Citrus tristeza virus isolates from orange trees showing symptoms of citrus sudden death disease" potx
... http://www.virologyj.com/content/5 /1/ 9 A B HM1 HM2 HM3 HM4 C3 HM5 C5 HM6 C6 HM7 HM8 HM9 HM10 H1 HM 11 HM12 HM13 C Japan (AB046398) Spain (DQ1 515 48) H2 % of population 10 0 80 16 15 11 16 60 94 40 74 68 20 H3 ... http://www.virologyj.com/content/5 /1/ 9 A B RM1 RM2 RM3 C3 RM4 Japan (AB046398) Spain (DQ1 515 48) Israel (AY206452) R1a C5 RM5 RM6 C6 RM7 US (AF0 016 23) Spain (Y18420) US (AF2606 51) R1b C R2 R3 US (U16304) US (NC 0 016 61) Egypt ... C6 US (AF2606 51) US (AF0 016 23) Japan (Y18420) US (NC 0 016 61) Spain (AY170468) Mexico (DQ272579) 10 0 US (U16304) (AY340974) Egypt 0.05 Figure Phylogenetic and recombination analysis of 284 HSP70h...
Ngày tải lên: 20/06/2014, 01:20
Báo cáo lâm nghiệp: "Changing electrophoretic patterns of glutamate dehydrogenases and aspartate aminotransferases in a few tree species under the influence of ectomycorrhization" potx
... ectomycorrhizas of spruce (Picea excelsa L.) and beech (Fagus sylvatica L.) New Phytol 11 1, 683-692 Khalid A., Boukroute A., Botton B & Martin F (19 88) The aspartate aminotransferase of the ectomycorrhizal ... Biochem 26, 17 -28 Kusnan M.B., Berger M.G & Fock H.P (19 87) The involvement of glutamine synthetase/glutamate synthase in ammonia assimilation by Aspergillus nidulans J Gen Microbiol 12 3, 12 35 -12 42 ... 12 35 -12 42 Lea P.J & Miflin B.B (19 74) An alternative route for nitrogen assimilation in higher plants Nature 2 51, 614 - 616 References Blumenthal K.H & Smith E.L (19 73) Nicotinamide adenine dinucleotide...
Ngày tải lên: 09/08/2014, 04:20
KINETIC ANALYSIS OF PRIMATE AND ANCESTRAL ALCOHOL DEHYDROGENASES
... 17 +/- 18 +/- 15 +/- 12 +/- 9.8 +/- 0.2 18 +/- 9.7 +/- 1. 9 12 +/- 12 +/- 12 +/- 12 +/- 10 +/- 18 +/- 9.8 +/- 0.8 23 +/- 22 +/- 29 +/- 16 +/- 17 +/- 35 +/- 10 12 +/- 22 +/- 15 +/- +/- +/- +/- 28 ... 0.09 1. 1 +/- 0 .1 1 .1 +/- 0 .1 0.84 +/- 0 .14 0.74 +/- 0.03 0.82 +/-0 .14 0.47 +/- 0.03 0.32 +/- 0.07 0.038 +/- 0.003 0.037 +/- 0.003 40 +/- 14 2.2 +/- 0.6 2M 10 M 0.79 +/- 0 .10 0.95 +/- 0 .12 1. 2 +/- ... -2-hexen -1- ol 0.66 +/- 0.04 Sigma 2-2 σσ2 310 +/- 73 370 +/- 13 31 +/- 8.3 +/- 0.8 2.2 +/- 0.6 0.34 +/- 0.02 28 1. 4 0.79 0.28 0 .13 0. 019 SUBSTRATES Table 11 : Vmax Constants (min -1) of Ancestral1 and...
Ngày tải lên: 24/08/2014, 12:25
TRANSCRIPTIONAL REGULATION OF THE HUMAN ALCOHOL DEHYDROGENASES AND ALCOHOLISM
... tract10 ,11 , fetal liver6 as mRNA in fetal and adult liver6 Common substrates ethanol12, retinol13 ethanol12, retinol13 ethanol12, retinol13 ethanol12, retinol13 HMGSH14 ,15 , GSNO16 retinol13, ethanol12 ... al., 19 96) 11 (Yin et al., 19 93) 12 (Edenberg and Bosron, 19 97) 13 (Yang et al., 19 94) 14 (Kaiser et al., 19 91) 15 (Koivusalo and Uotila, 19 91) 16 (Staab et al., 2008) 10 The seven ADH isozymes ... al., 19 72) (Smith, 19 86) (Duley et al., 19 85) (Allali-Hassani et al., 19 97) (Estonius et al., 19 96) (Yin et al., 19 90) (Yokoyama et al., 19 95) (Zgombic-Knight et al., 19 95) (Dong et al., 19 96) 11 ...
Ngày tải lên: 24/08/2014, 12:37
EMBEDDED LINUX SYSTEM DESIGN AND DEVELOPMENT.pdf
... 1. 1 .1 Year 19 99 1. 1.2 Year 2000 1. 1.3 Year 20 01 1. 1.4 Year 2002 1. 1.5 Year 2003 1. 1.6 Year 2004 1. 2 Why Embedded Linux? 1. 2 .1 Vendor ... Linux 10 1. 4 Frequently Asked Questions 11 1. 4 .1 Is Linux Too Large? 11 1. 4.2 Is Linux Real-Time Enough? 11 1. 4.3 How Can I Protect My Proprietary Software? 12 1. 4.4 Should ... Devices 11 4 4.7 Mtdutils Package 11 6 4.8 Embedded File Systems 11 6 4.8 .1 Ramdisk 11 7 4.8.2 RAMFS 11 7 4.8.3 CRAMFS (Compressed RAM File System) 11 7 4.8.4 Journaling...
Ngày tải lên: 04/08/2012, 14:23
09 Physical and Chemical Characteristics of DDGS revisions.
... Mean 13 98 13 22 14 25 13 70 12 55 612 974 12 58 11 42 13 37 14 88 12 35 11 98 12 29 212 5 11 48 12 82.25 Standard Deviation 2.32 2.00 1. 62 1. 84 1. 68 2.75 2 .15 1. 70 1. 84 1. 78 1. 62 1. 75 1. 87 2.09 1. 56 2.25 1. 93 ... ppb 16 -Mar-04 17 -Mar-04 24-Mar-04 31- Mar-04 7-Apr-04 14 -Apr-04 21- Apr-04 28-Apr-04 5-May-04 12 -May-04 19 -May-04 27-May-04 3-Jun-04 10 -Jun-04 10 11 12 9.05 10 .17 10 .65 10 .70 10 . 71 10.76 10 .93 11 .02 ... 36.8 36.3 33.5 39.3 36 .1 33.7 30.8 31. 8 38.2 31. 4 35.9 39.2 37.6 35 .1 35.7 CV % 0 .17 0 .15 0 .11 0 .13 0 .13 0.45 0.22 0 .14 0 .16 0 .13 0 .11 0 .14 0 .16 0 .17 0.07 0.20 0 .15 68% of the particles will fall...
Ngày tải lên: 08/08/2012, 10:03
CHECK YOUR ENGLISH VOCABULARY FOR BUSINESS AND ADMINISTRATION.pdf
... Dictionary of Business 4th edition (A&C Black 978-0- 713 -67 918 -2) 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 42 41 43 45 44 46 47 48 49 50 51 52 53 ... less profit than another company) 33 For reference see Dictionary of Business - 4th edition (A&C Black Publishers Ltd, 978-0- 713 -67 918 -2) R U N W I T H 10 11 12 13 14 15 16 17 18 19 20 21 22 23 ... Contents Page: Title: 1 2 3–5 6–7 – 10 11 – 12 13 – 14 15 16 – 17 18 – 19 20 – 22 23 – 24 25 – 27 28 29 – 30 31 32 – 34 35 36 – 38 39 40 – 41 42 – 43 44 – 45 46 47 – 48 49 – 50 51 – 52 53 – 54 55...
Ngày tải lên: 08/08/2012, 17:31
Check Your English Vocabulary for Phrasal Verbs and Idioms.pdf
... Page: Title: 6–7 8–9 10 11 – 12 13 – 14 15 – 16 17 – 18 19 – 20 21 – 22 23 – 24 25 26 – 27 28 29 – 30 31 – 32 33 – 34 35 – 36 37 – 38 39 40 41 – 42 43 44 – 45 46 47 48 – 49 50 – 51 52 – 53 54 55 – ... interesting He's just a bit of eye _ 10 11 12 13 14 16 19 17 18 20 21 22 23 24 25 26 22 15 Unit 'get' Phrasal verbs, idioms and other expressions using 0000 Exercise 1: Choose the correct particle ... a _ C U E K I K E O C R N L 10 T 11 F 12 E T G G 13 14 I T E 15 E 16 L 18 I I 21 P I 22 24 25 T O 26 28 30 A E 29 O U Y 20 R D L 27 E Z 31 32 Y N I 23 H 19 G 20 17 E Idioms and other expressions...
Ngày tải lên: 08/08/2012, 17:32
Atmel AVR Microcontroller Primer Programming and Interfaceing
... 7-(MISO) PB6 8-(SCK) PB7 sys reset 9-RESET 10 -Vcc 11 -GND 12 -XTAL2 13 -XTAL1 14 -(RXD) PD0 15 -(TXD) PD1 16 -(INT0) PD2 17 -(INT1) PD3 18 -(OC1B) PD4 19 -(OC1A) PD5 20-(ICP) PD6 PORTB VDD = VDC PORTA ... detail in Chapter 13 FIGURE 1. 4: ATmega16 Testbench hardware SW7 SW0 0 .1 uF 470K 4.7K 0 .1 uF VDD = VDC 470K 4.7K 74HC14 74HC14 VDD 1. 0 uF ZTT 10 MHz resonator 1M PA0 (ADC0)-40 PA1 (ADC1)-39 PA2 (ADC2)-38 ... debounced Reset 10 VDD 11 Gnd 12 Resonator 13 Resonator 14 PD0 to tristate LED indicator 15 PD1 to tristate LED indicator 16 PD2 to tristate LED indicator 17 PD3 to tristate LED indicator 18 PD4 to...
Ngày tải lên: 16/08/2012, 09:03
Pro WPF and Silverlight MVVM effective Application Development
... 10 4 Updating the UI 10 7 Summary 11 0 ■Chapter 5: Events and Commands 11 1 Events 11 1 Events in NET 11 1 Events in ... Silverlight 11 2 Commands 11 5 Command Pattern 11 6 The RelayCommand 11 9 Attached Command Behavior 12 6 Avoiding Events Using ... written permission of the copyright owner and the publisher ISBN -13 (pbk): 978 -1- 4302- 316 2-2 ISBN -13 (electronic): 978 -1- 4302- 316 3-9 Printed and bound in the United States of America Trademarked...
Ngày tải lên: 17/08/2012, 08:54