1 25 oh 2d3 in calcium phosphorus and bone metabolism

... ††,‡‡ 11 .6 ± 2.8 (47) 11 .8 ± 2.7 (45) 1. 1 ± 1. 3 ( 41) 0.8 ± 0.8 (36) 0.9 ± 1. 1 (34) ‡ 1. 2 ± 1. 4 (44) 1. 3 ± 1. 5 (46) 1. 1 ± 0.9 (44) HRT 47.7 ± 47.9 ( 31) 17 7.6 ± 13 9.4 (25) †††,‡‡‡ 17 6 .1 ± 12 4.0 ( 31) ... ESR - 0 .11 1 0.280* 0.088 0.026 0 .257 * 0.095 -0 .17 1 -0 .19 5 -0 .10 0 0.500*** CTX-I 0 .11 1 - 0.366** 0 .19 4 0.4 01* ** 0 .12 9 0.099 -0.293* -0 .253 * -0 .18 7 0 .18 2 ICTP 0.280* 0.366** - -0 .12 0 0 .13 8 0.472*** ... obtained at baseline and after 12 and 24 months Forty joints in the hands and feet were scored (in the hands, proximal interphalangeal joints of digits 1 5, metacarpophalangeal joints of digits 1 5,...

Ngày tải lên: 09/08/2014, 01:24

... containing bone morphogenetic protein (BMP-2) and/ or nanoparticles of hydroxyapatite (nHAp) using electrospinning, and showed its ability in inducing in vitro bone formation from human bone marrow-derived ... stem cell, and developmental biology, as well as materials science and bioinformatics (Donald and Mohammad, 20 01) Chapter 1. 1 .1 Cells Tissue engineering utilizes living cells as engineering materials ... 225- 230 35 ~ 40 7.0 ~ 12 L-PLA 17 3 -17 8 60 ~ 65 2.7 > 24 DL-PLA Amorphous 55 ~ 60 1. 9 12 ~ 16 PCL 58-63 -65 ~ -60 0.4 > 24 PDO N/A -10 ~ 1. 5 ~ 12 PGA-TMC N/A N/A 2.4 ~ 12 85 /15 DL-PLGA Amorphous...

Ngày tải lên: 14/09/2015, 08:41

... Lesions in the thymus and bone marrow in chicks with CIA 19 Table Continued Days Bird No ELISA test results 14 E1 E2 E3 E4 C1 C2 E1 E2 E3 E4 C1 C2 E1 E2 E3 E4 C1 C2 E1 E2 E3 E4 C1 C2 E1 E2 E3 E4 C1 ... group (n) 1- 7 8 -10 12 -14 17 -20 25- 28 30 † Control group (n) *46.84 ± 3,049d,A (12 ) 51. 60 ± 3,606d,A (8) 65.78 ± 6,225c,A (12 ) 71. 73 ± 10 ,253 c,A (8) 80.75 ± 12 ,824b,A (8) 13 5.20 ± 11 ,703a,A (4) d,A ... and significant values of the data obtained were determined by Duncan’s test [3], as groups comprising the days 1- 7, 8 -10 , 12 -14 , 17 -20, 25- 28, and 30 Lesions in the thymus and bone marrow in...

Ngày tải lên: 07/08/2014, 20:23

... F4 (cm -1) 16 47 -16 60 α-Helix 12 1 9±2 11 ±2 11 ±2 12 1 1 615 -16 36 β- sheet 26±4 19 ±7 24±6 26±2 25 2 16 37 -16 46 random 10 1 10±3 10 1 10±2 11 1 1660 -16 80 Turn 26±2 38 18 30±2 29 1 26±2 16 81- 1692 β- ... lie within the range 16 47 -16 60cm -1 For β-sheet, the peak should lie within the range 16 15 -16 36cm -1 For antiparallel β-sheet, the range should be within 16 81- 1692cm -1 As for turn and random structure, ... Bio-Rad Mini-PROTEAN electrophoresis system (Cat No: 16 5-33 01 and 16 5-3302) at a constant voltage difference (10 0V) Protein bands were detected by Coomassie G -250 staining using GelCode Blue Stain...

Ngày tải lên: 14/09/2015, 08:41

... Pfeilschifter et al., 19 93; Scherping et al., 19 97; Thaller et al., 19 93) In particular, BMPs are osteoinductive proteins originally identified in demineralized bone (Urist and Nogami, 19 70) They are ... growth factor and insulin growth factor have been shown to stimulate bone growth, collagen synthesis, and fracture repair both in vitro and in vivo (Jingushi et al., 19 95; Nixon et al., 19 98; Pfeilschifter ... facilitate bone healing without transferring bone tissues Among this group of proteins, BMP-2 has been shown to induce healing in segmental bone defects Aebli and colleagues (Aebli et al., 2005) and...

Ngày tải lên: 14/09/2015, 08:41

... 60 and 85% for A1, A2 and A3 respectively), 30% (18 , 31 and 50% for B1, B2 and B3 respectively) and 16 % (8, 16 and 24% for C1, C2 and C3 respectively) of the DNA is released after days This finding ... 277 249 19 5 2 01 297 252 13 3 ±50 ±38 ±39 ±29 ± 21 16 ±57 ±28 ±24 10 0 10 0 10 0 10 0 10 0 10 0 65±5 78±9 87±4 Encapsulation Efficiency (%) Chapter Mode A Mode B Mode C Figure 5 .1 Three DNA incorporation ... 11 0 Cumulative Percentage (%) 10 0 90 80 70 60 50 B1 B2 B3 40 30 20 10 0 12 18 24 30 36 42 48 54 60 66 Time (days) (b) 11 0 Cumulative Percantage (%) 10 0 90 80 70 60 50 C1 C2 C3 40 30 20 10 0 12 ...

Ngày tải lên: 14/09/2015, 08:41

... w/w) A1 A2 A3 B1 B2 B3 C1 C2 C3 0 /10 0 5/95 10 /90 0 /10 0 5/95 10 /90 0 /10 0 5/95 10 /90 6.2.4 In vivo experiments All procedures were performed in accordance to specifications in the Guidelines for ... healing is observed for samples A1, B1, B3, and C1 Furthermore, this also confirms that during the progress of bone healing, tissue reabsorbing/remodeling, and the in growth of blood vessel are interactively ... of the color and particularly significant for samples A1, B1, B3, and C1 These results demonstrate that the cells in bone fragments treated by A1, B1, B3 and C1 experienced good and healthy neovascularization,...

Ngày tải lên: 14/09/2015, 08:41

... Chitosan crystals 25 50 75 10 0 12 5 15 0 17 5 200 225 250 275 300 325 350 375 400 o Tem perature ( C ) (a) F0 F1 F2 Chitosan crystals 5.0 7.5 10 .0 12 .5 15 .0 17 .5 20.0 22.5 25. 0 -1 Frequency ( cm ... are maintained after going through the processes of spray drying, supercritical CO2 foaming and post-processing conditions (handling of scaffold, incubations, and lyophilization) As shown in Figure ... V2 Vent 10 mm CO2 V1 C1 HP 10 mm P1 M Figure 7 .1 Schematic of experimental setup for the supercritical gas foaming system (C1) Refrigerating Circulator; (C2) Circulating water bath; (P1) High pressure...

Ngày tải lên: 14/09/2015, 08:41

... 4.99 2.23 49.60 13 .48 23.03 13 .89 F2/DNA 57. 61 35.68 4.82 1. 89 49.27 13 .11 22. 51 15 .11 16 8 Chapter F0 C 1s C-C C-O O-C=O 292 290 288 286 284 282 280 Binding energy (eV) (a) C 1s F1 C-C C-O C-N ... 16 9 Chapter F0 F1 F2 408 406 N 1s 404 402 400 398 396 394 392 Binding energy (eV) (a) F0 F1 F2 14 0 13 8 P 2p 13 6 13 4 13 2 13 0 12 8 12 6 12 4 Binding energy (eV) (b) Figure 8.3 (a) N1s high-resolution ... 66.6 ± 3 .1 1.03±0 .17 F1: K4 conjugated PLGA 76.3 ± 2.9 4.32±0 .13 F2: K20 conjugated PLGA 78.8 ± 2.5 3. 81 0 .12 Table 8.2 Atomic composition (C1s, O1s, N1s and P2p) and percentage of C1s in XPS spectra...

Ngày tải lên: 14/09/2015, 08:41

... PLGA/chitosan and PLGA/Lysine composites is in form of naked DNA and its transfection efficiency is extremely low, which would hinder their applications in biomedical engineering In future investigations, ... cytotoxicity and sustained expression of DNA, which is favorable for DNA delivery and tissue engineering applications In conclusion, this research has shown potential applications of electrospinning and ... was well maintained, which helped improve the formation of new bone and the healing of segmental defects in vivo Groups A, B and C released DNA or DNA nanoparticles in different ways and time frames...

Ngày tải lên: 14/09/2015, 08:41

... 9.4 1. 0 10 .7±0.80 9.4±0.6 11 .0±0.9* 8.3 1. 2 10 .8 1. 0* 8.6±0.9 11 .5 1. 0* 8.3 1. 00 8.5±0.8 9 .1 0.6 10 .0 1. 10 11 .1 1. 1** 11 .1 0.9* 10 .3±0.7* 9.6±0.5 3.3±2.0 5.8 1. 2 3.5 1. 40 6.3 1. 5* 2.5 1. 5 06.0 1. 9* ... 1, 25( OH) D , Ca, iP and Mg concentrations around parturition in cows of 1, 25( OH) D and control groups Plasma 1, 25( OH) D concentrations in 1, 25( OH) D group (3 91. 4 ± 18 8.9 to 11 85.0 ± 384 .1 pg/ml) were ... 2.4±0.2 -1 -0.5 0.5 53.6±22 .1 52.9 14 .300 73.3±35.300 75.4±23.80 11 85.0±384 .1* 694.5±526.7** 596.6±338.4** 3 91. 4 18 8.9* 96.6 25. 9 95.8± 31. 7 95.4±23.0 57.4 16 .20 51. 9±59 .1 65.2 17 .5 45.9 11 .6 20.2 12 .5*...

Ngày tải lên: 07/08/2014, 18:21

... after 1, 25- (OH) 2D3 treatment, the number of podocytes and FPW in 1, 25- (OH) 2D3 group was higher and smaller than those in ADR group, respectively (Table and Figure 1) n 24 h UP (mg) 16 1. 01 0.29 15 ... version 11 .0 Group control ADR ADR +1, 25( OH) 2D3 n 16 15 16 Podocytes 5.46±0. 51 3.35±0 .14 ## 4.44±0.23## △△ Data are means ± SD FPW, foot process width FPW (nm) 2 71. 38±52.48 806 .13 12 0 .19 ## 4 01. 13±52.48## ... 0.68±0 .10 , 1. 10±0.20, 0 .12 ±0.08, 0 .12 ±0.06, 0.24±0.09, and 0.86 ± 0 .15 , respectively, in the control group, 1. 04±0.22, 0.79±0 .12 , 0.52±0 .13 , 0.44±0 .11 , 0. 61 0 .12 , and 0.23 ± 0.07, respectively, in...

Ngày tải lên: 25/10/2012, 11:40

... +vehicle CLP + 1, 25 vit-D 647 ± 95 Control + 1, 25 vit-D 606 ± 13 9 418 ± 49 b 515 ± 53 c 12 ,0 ± 0,2 12 ,2 ± 0,7 11 ,4 ± 0,6 11 ,0 ± 0,8 12 ,5 ± 1, 2 12 ,7 ± 1, 0 53 ± 53 ± 59 ± 13 64 ± 71 ± 17 77 ± 13 b 0,68 ... a 12 ,0 ± 0,4 53 ± 53 ± 12 4 ± 16 7 94 ± 24 a 0,68 ± 0,29 0,62 ± 0,27 1, 77 ± 1, 10 2 ,12 ± 0,53 a 41 ± 46 ± 19 44 ± 57 ± 11 8,2 ± 0,5 8,4 ± 0,7 10 ,66 ± 3,09 11 ,90 ± 2,47 a 1, 34 ± 0,02 1, 36 ± 0,02 1, 36 ... and activity [11 ,12 ,13 ] The inflammatory cytokines, TNF-alpha and certain interleukins play a key-role in initiating systemic inflammatory response syndrome (SIRS) and it is known that vitamin...

Ngày tải lên: 26/10/2012, 09:57

... significant increase in integrin alpha6 and decrease in integrin alpha v and beta4 expression levels In the presence of fibronectin and/ or HGF, a significant increase in integrin alpha3 and alpha5 expression ... sphingosine -1- phosphate and sphingosylphosphorylcholine can mobilize calcium from internal sources, that sphingosine kinase binds to and is possibly regulated by calmodulin, and that sphingosine is an inhibitor ... with SR1 417 16 (a CB1 antagonist), proving that the CB1 receptors are fully functional The GABAB agonists baclofen and SKF 975 41 also elevated [35S] GTPcS binding by 14 9 and 18 6%, respectively Interestingly,...

Ngày tải lên: 19/02/2014, 07:20

... representing fulllength CCN4 ⁄ WISP1 mRNA and WISP1v were Finally, the biological function of WISP1v and WISP1vx was evaluated using overexpression experiments FLAG epitope-tagged WISP1v and WISP1vx ... 10 80 10 89 11 Luo X, Ding L & Chegini N (2006) CCNs, fibulin-1C and S100A4 expression in leiomyoma and myometrium: inverse association with TGF-beta and regulation by TGF-beta in leiomyoma and myometrial ... and adhesion Mol Cell Biol 16 , 13 26 13 34 Leu SJ, Liu Y, Chen N, Chen CC, Lam SC & Lau LF (2003) Identification of a novel integrin alpha beta binding site in the angiogenic inducer CCN1 (CYR 61) ...

Ngày tải lên: 07/03/2014, 10:20

... Adam-Vizi 10 3 10 4 10 5 10 6 10 7 10 8 10 9 11 0 11 1 11 2 11 3 11 4 tion, release of cytochrome c and cell death Correlation with the duration of pore openings in situ J Biol Chem 276, 12 030 12 034 Di Lisa ... [14 5 ,14 9 ,15 0 ,15 5] Results supporting views that DCD is comprised of an active Ca2+ in ux pathway [93 ,14 6 ,14 9 ,15 0 ,15 5 15 9] as well as those indicating a failure in Ca2+ efflux mechanisms [16 0 16 2], ... Flow Metab 10 , 13 6 13 9 19 2 Hansen AJ & Zeuthen T (19 81) Extracellular ion concentrations during spreading depression and ischemia in the rat brain cortex Acta Physiol Scand 11 3, 437– 445 19 3 Martell...

Ngày tải lên: 07/03/2014, 12:20

... F (19 98) Ó FEBS 2004 4494 M Pastorcic and H K Das (Eur J Biochem 2 71) 10 11 12 13 14 15 16 17 18 19 20 21 Deficiency of presenilin -1 inhibits the normal cleavage of amyloid precursor protein Nature ... 7 15 ) and +10 7/ +17 8 (lanes 16 –24) Either the wild type probes or the same fragments containing mutant Ets sites at positions )10 (lanes 4–6), +90 (lanes 10 12 ), +65 (lanes 13 15 ), +12 9 (lanes 19 – 21) ... binding of nuclear factors is specifically competed by a heterologous Elk1 binding site The specific binding in the promoter regions )22/+6 (lanes 1 6), +50/ +10 7 (lanes 7 18 ), +10 7/ +17 8 (lanes 19 –...

Ngày tải lên: 16/03/2014, 18:20

... cells were maintained in RPMI -16 40 medium supplemented with 10 % (v ⁄ v) fetal bovine serum and 10 0 UÆmL )1 penicillin and 10 0 lgÆmL )1 streptomycin [46] The cells were incubated at 37 °C in a humidified ... FEBS 618 9 1, 2-Diarachidonoyl-glycerophosphoinositol 13 14 15 16 17 18 19 20 21 22 23 D Balgoma et al phospholipase A2 in arachidonic acid release, phospholipid fatty acid incorporation, and apoptosis ... phospholipid content and composition, insulin secretion, and proliferation of INS -1 insulinoma cells J Biol Chem 2 81, 18 7 19 8 Lio YC & Dennis EA (19 98) Interfacial activation, lysophospholipase and transacylase...

Ngày tải lên: 23/03/2014, 06:20

... & Leung, L.L (19 99) Thrombin interacts with thrombomodulin, protein C, and thrombin-activatable fibrinolysis inhibitor via specific and distinct domains J Biol Chem 274, 25 510 25 516 Stockmann, ... PAI -1 alone (ki  10 5 M )1 s )1) Therefore, the inhibitory effect of TM on the inhibition of 15 nM thrombin by preincubated PAI -1/ VN complexes (10 0 nM PAI and 15 0 nM VN) was determined (Fig 1D) ... Thrombomodulin sterically blocks the thrombin/PAI -1 interaction (Eur J Biochem 270) 19 51 15 Rezaie, A.R (19 99) Role of exosites and in thrombin reaction with plasminogen activator inhibitor -1 in the...

Ngày tải lên: 23/03/2014, 17:21

... Ser577 in SIK1 and Ser587 in SIK2, are indicated in bold The basic residues in domain 3B are indicated by underlines The similarity between SIK1 and SIK2 in domain 3A, the N-terminal half of domain ... Okamoto, M (20 01) Salt-inducible kinase is involved in the ACTH/cAMP-dependent protein kinase signaling in Y1 mouse adrenocortical tumor cells Mol Endocrinol 15 , 12 64 12 76 Takemori, H., Katoh, Y., Horike, ... 17 957 17 960 13 Wu, X & McMurray, C.T (20 01) Calmodulin kinase II attenuation of gene transcription by preventing cAMP response 17 18 19 20 21 22 23 24 25 26 27 28 29 element-binding protein (CREB)...

Ngày tải lên: 30/03/2014, 15:20