1 25 dihydroxyvitamin d and its health implications

... increases mitochondrial membrane permeability http://www.medsci.org Int J Med Sci 2 010 , 7 10 11 12 13 14 15 16 17 18 19 20 21 22 and is inhibited by Bcl-2 J Biol Chem 2006; 2 81( 21) : 14 764 -14 775 Makibayashi ... of renal cortex was fixed in 1. 5% glutaraldehyde and 1% paraformaldehyde, dehydrated, and embedded in Spurr resin Ultrathin sections were prepared and stained with lead citrate for transmission ... 50 kDa, 25 kDa, 23 kDa, 26 kDa and 36 kDa, respectively The expressions of p-Smad2/3, p-Smad1/5/8, Fas, FADD, Bax, and Bcl-2 were 0.68±0 .10 , 1. 10±0.20, 0 .12 ±0.08, 0 .12 ±0.06, 0.24±0.09, and 0.86...

Ngày tải lên: 25/10/2012, 11:40

... 10 4.75±20.55 Diabetic control group 300.63 10 4.69a 244 .13 10 7.03b 222.75 10 4. 81 b 2 01. 38± 91. 64 b 209.50±87. 61 a 203.75 10 0.30 a 18 0 .25 11 1.82 b Diabetic treated group 294 .13 ±89.97a 208 .13 ±76.43 17 0.00±76. 91 ... short peptide, 15 amino acids), and gAd (13 7 amino acids) (Fig 1A) The fuhttp://www.medsci.org Int J Med Sci 2 011 , sion protein was absent in non-induced condition SDS-PAGE analysis showed that most ... The refolded protein was incubated with enter- 205 okinase (1U enterokinase was added in 0.5mg refolded protein) at 22°C for 16 h to produce gAd-GLP -1- A fusion protein The digested products were...

Ngày tải lên: 25/10/2012, 11:10

... Languages 25 (2009) 257 -263 [11 ] D Wall, Impact and washback in language testing, In C C & D Corson (Eds.), Encyclopaedia of language and education, Language Testing and Assessment (19 97) 2 91 [12 ] ... abilities, University of London Press, London, 19 96 [19 ] J Fish, Responses to mandated standardised testing Unpublished doctoral dissertation, University of California, Los Angeles, 19 88 [20] M.L Smith, ... and at macro-level (educational and societal system), Tables and is presented below for classification Evaluation notes were added to the output document To get rid of these notes, please order...

Ngày tải lên: 05/03/2014, 12:20

... 0 .13 7 0.2 01 0 .14 8 0.079 0.079 0. 216 0.386 0. 218 0 .13 6 0 .11 3 0 .15 1 0.057 0.063 1. 913 0.835 1. 122 2.360 0.684 0.987 0. 425 1. 574 1. 300 1. 640 1. 811 0.933 2 .10 4 0.860 ± ± ± ± ± ± ± 0.2 41 0.035 0.073 ... lM) and Ac-DEVD-CHO (2 lM) (8) Ab (25 35) (20 lM) and compound (25 lM) (9) Ab (25 35) (20 lM) and compound (25 lM) The data indicated isoquinoline -1, 3,4-trione reduced this cytotoxicity induced by ... increased to 19 . 91% and 31. 71% In contrast, apoptosis ratios were 4. 01% and 4.09% when PC12 cells were treated with lm Ac-DEVDCHO and 28 lm compound 1, respectively Apoptosis ratios were 2.27% and...

Ngày tải lên: 07/03/2014, 11:20

... 61. 1 1, 014 24 25. 0 67.3 2, 218 547 11 1 3 ,15 6 7 01 1.5 0.4 1, 333 1, 633 22 37.5 67.7 2 ,18 3 18 7 10 1 54 5 41 811 18 1 2.7 1. 8 0.7 2,400 10 0 1, 000 93 44 65 11 .7 52.0 21. 0 73.6 61. 0 67.5 22,6 61 1,490 ... the world, e.g., extended periods of extreme heat and cold [9] may be long enough for diseases to develop [32] Record extremes of high and low temperature, and drought and flood are already being ... Islands 16 237 0.3 −98 72 11 .6 72.8 10 ,875 French 269 3,5 21 1.2 51 5.8 74 .1 21, 0 71 Polynesia Niue 259 −2.3 −42 36 7.8 71. 6 9, 618 Pitcairn Islands – – – – – – – Samoa 18 4 2,935 0.3 −3,050 21 20.4...

Ngày tải lên: 29/05/2014, 23:42

... 9.4 1. 0 10 .7±0.80 9.4±0.6 11 .0±0.9* 8.3 1. 2 10 .8 1. 0* 8.6±0.9 11 .5 1. 0* 8.3 1. 00 8.5±0.8 9 .1 0.6 10 .0 1. 10 11 .1 1. 1** 11 .1 0.9* 10 .3±0.7* 9.6±0.5 3.3±2.0 5.8 1. 2 3.5 1. 40 6.3 1. 5* 2.5 1. 5 06.0 1. 9* ... plasma 1, 25( OH) D , Ca, iP and Mg concentrations around parturition in cows of 1, 25( OH) D and control groups Plasma 1, 25( OH) D concentrations in 1, 25( OH) D group (3 91. 4 ± 18 8.9 to 11 85.0 ± 384 .1 pg/ml) ... inhibition of renal 1- α-hydroxylase and endogenous 1, 25( OH) D synthesis by exogenous 1, 25( OH) D administration [7,8] Repeated administration of low dose of 1, 25( OH) D as described by Hoffsis et al...

Ngày tải lên: 07/08/2014, 18:21

... providing the Tat protein in a highly pure form, and to Michel Léonetti (CEA, Saclay, France) for the monoclonal anti-Tat 7S and 11 S antibodies References 10 11 12 13 14 15 16 17 18 19 20 21 Strebel ... structure and corrected the manuscript RSR contributed to the study design and constructed some of the plasmids used in this study TO and MLL assisted with manuscript preparation and edition JLD contributed ... ODNs (Table and Additional file 1) and the Vent polymerase (New England Biolabs, Ipswich, MA, USA) Then, reconstitution of the Tat1 and Tat2 DNA fragments was carried out (Fig 1B): minus strand...

Ngày tải lên: 12/08/2014, 23:22

... 1. 1.4 Subtypes of PPARs 1. 1.5 Ligands and physiological functions of PPARα and PPARδ 1. 1.6 Ligands of PPARγ 1. 1.7 PPARγ and adipogenesis 10 1. 1.8 PPARγ and ... 12 1. 1.9 PPARs and cancer 13 1. 1 .10 PPARγ and breast cancer 15 1. 2 ESTROGEN RECEPTORS (ERS) 18 1. 2 .1 Identification and structures of ERs 18 1. 2.2 Mechanism ... is induced upon binding of various ligands including saturated and unsaturated fatty acids, arachidonic acid derivatives and different synthetic compounds Since the discovery of thiazolidinedione...

Ngày tải lên: 09/09/2015, 10:19

... then determined by Western blot, NHE1 band intensity was normalized to β-actin (D) MCF-7 (3 X105 cells/6-well dishes), MDA-MB-2 31 (2X105 cells/6-well dishes) and T4 7D cells (3 X105 cells/6-well dishes) ... nuclear lysis buffer and biotinylated double-stranded oligonucleotides was included The fluorescence intensity corresponded to the amount of PPARγ bound to biotinylated double-stranded PPRE As shown ... 10 6 /10 0mm dish) and (B) MDA-MB-2 31 (1. 7 X 10 6 cells /10 0mm dish) cells were exposed to 3M 15 d- PGJ2 for 4h before harvesting for nuclear-cytosol fractionation as described in Materials and Methods...

Ngày tải lên: 09/09/2015, 10:19

... seeded in RPMI supplemented with CS-FBS, and deprived before E2 was added The cells were 12 9 then exposed to increasing doses of 15 d- PGJ2 after 2h Total cell lysate was harvested after 24h and ... NHE1 was 13 2 analyzed by Western blot NHE1 band intensity was normalized to β-actin (C) MDA-MB-2 31 (2X10 cells/6-well dishes) and MDA-MB-2 31 ERα+ (2X105 cells/6-well dishes) cells were treated ... silenced the NHE1 gene in MDA-MB-2 31 cells using siRNA specific for NHE1 gene The transfected cells were replated onto 10 0mm dish and kept for 14 days, before the colonies formed were stained 11 9...

Ngày tải lên: 09/09/2015, 10:19

... comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5 (and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 12 3." Free Radic Biol Med 27 (1- 2): 14 6 -15 9 Hess, R., W ... obese and NIDDM patients." Diabetes 46(8): 13 19 -13 27 Babior, B M (19 99) "NADPH oxidase: an update." Blood 93(5): 14 64 -14 76 Badawi, A F and M Z Badr (2003) "Expression of cyclooxygenase-2 and peroxisome ... end, MCF-7, MDA-MB-2 31 and T4 7D cells were treated with different doses of synthetic ligands as well as natural ligand of PPARγ, and the protein expression and mRNA transcript level of NHE1 were...

Ngày tải lên: 09/09/2015, 10:19

... stained with crystal violet (C) MDA-MB-2 31 (2 X 10 5 cells/6-well dishes) and T4 7D (3 X105 cells/6-well dishes) cells were treated with 15 d- PGJ2, for 16 h before they were washed and reseeded at a density ... X10 cells/6-well dishes) cells were exposed to (A) 15 d- PGJ2 and (B) ciglitazone for 16 h before they were washed and reseeded at a density of 1. 0 X104 cells /10 0mm dish After 14 days of incubation, ... exposed to 15 d- PGJ2 or ciglitazone for 16 h, before equal number of cells were replated onto 10 0mm dish and maintained for 14 days After crystal violet staining, we observed a dose-dependent reduction...

Ngày tải lên: 09/09/2015, 10:19

... NHE1in MDA-MB-2 31 cells kept in regular serum condition (A) Endogenous NHE1 mRNA expressions in MDA-MB-2 31 and MDA-MB-2 31 ERα+ were determined by Taqman real-time PCR, normalized to the endogenous ... receptor and 14 5 c-Myc were determined in MCF-7 cells exposed to 10 nM fulvestrant and 10 0nM raloxifene As shown in figure 23A, both drugs induced a reduction in expressions of c-Myc and progesterone ... NHE1 expression to PPARγ ligand In MCF-7 cells transfected with plasmid encoding DNA-binding defective ERα receptor, 15 d- PGJ2 resulted in dose-dependent reduction in NHE1 protein expression (Figure...

Ngày tải lên: 09/09/2015, 10:19

... transport and metabolism 1cd2, 2drc, 1dls, 1dhi, 3drc, 3dfr, 1vif, 1dr1, 2blc, 1ia2, 1ly4, 1sej, 3jsu, 1dg5, 2w9s, 1j3j, 2w3w, 1bzf, 3clb, 2dhf, 1qzf, 1d1 g, 3k2h, 1dyh, 3frd, 3nrr, 1u70, 3ix9, ... 2ogi, 1yre, 2d1 y, 1p0h, 1q6y, 2gf6 3.358 0.2799 3 .19 7 0.2906 1emd, 3i0p, 1emd, 1hex, 1o6z, 1ur5, 3gvh, 1b8v, 1bmd, 1z2i, 2dfd, 2x0i, 1guz, 1gv0 3 .14 1 0.2653 1jmf, 1tlc, 1b02 3 .13 2 0.522 3.082 0. 616 3 ... activity 1ta8, 3bac, 3ba9, 3bab 3 .11 4 0. 31 3 .11 2 0.343 3.06 0.364 13 14 17 Involved in nucleic acid binding 18 17 2 Ribonuclease 1goy, 1rpf, 1w4q, 3dgy, 1s9g, 1rca, 1i0v,1rga, 2rok, 1rds, 3lpz, 1iyb...

Ngày tải lên: 10/09/2015, 15:49

... 7.0±0.9 15 2e 4-BrC6H4 2.0±0.5 14 .7 1. 3 15 2f 4-CF3C6H4 2.0 1. 9 15 .0 1. 7 15 2g 4-CNC6H4 13 .0±4.0 2.0±0.7 15 2h 2-Furyl 16 .0 1. 5 11 .1 0.9 15 2i 2-Thienyl 9.0 1. 5 10 .1 0.9 15 2j 2-Pyridyl 6.0±0.5 11 .7±0.4 ... Method A) There was a need to develop an alternative method of obtaining substituted dihydropyrimidin-2-yl)guanidines 12 8 -13 1 as the obtained yields of biguanides 13 2ad synthesized by Curd and Rose’s ... NR1R2 = NH2, 12 8 NR1R2 = NHCH3, 12 9 NR1R2 = N(CH3)2, 13 0 NR1R2 = morpholine, 13 1 Method A Scheme 39: Synthesis of 1- substituted-3-(4-methyl-6-oxo -1, 6-dihydropyrimidin-2yl)guanidines 12 8 -13 1 Reagents...

Ngày tải lên: 10/09/2015, 15:49

... has rocketed from 18 6 in 19 78 to 211 9 in 2003 43 Figure 3.3 Number of newspapers in China, 19 78-2003 250 0 2000 15 00 10 00 500 20 03 20 02 19 99 19 95 19 93 19 92 19 86 19 85 19 84 19 78 Source: ... incorporated the above ideas in their works During the 19 80s and 19 90s, ‘deregulation’ became a buzzword in the western media sector The ‘deregulation’, by the shift towards the market-driven media and ... 20 years Figure 3.2 Advertising Revenues of the Newspapers in China, 19 83-2004 12 0 10 0 80 60 40 20 19 83 19 85 19 87 19 89 19 91 1993 19 95 19 97 19 99 Sources: China Advertising (19 84- 2000), various...

Ngày tải lên: 26/11/2015, 22:58

... models (Jain 19 84; Odgaard 19 86; Hite and Mih 19 94) and physical models (Anwar et al 19 78; Jain et al 19 78; Yildirim and Kocabas 19 95; Yildirim and Kocabas 19 97; Yildirim and Kocabas 19 98) Many ... spherical coordinates J Hydr Engrg., ASCE, Vol 12 2(9), pp 512 -5 21 21 Ponce V M and Yabusaki S B 19 81 Modeling circulation in depth-averaged flow J Hydr Div., ASCE, Vol 10 7 (11 ), pp 15 01- 1 518 22 Sivakumaran ... 19 99 Bend-flow simulation using 2 -D depth-averaged model J Hydr Engrg., ASCE, Vol 12 5 (10 ), pp 10 97 -11 08 17 McGuirk, J J and Rodi, W 19 78 A depth-averaged mathematical model for the near field...

Ngày tải lên: 06/11/2012, 10:35

... in 19 th century together with its world’s leading industrial and trading role and its political imperialism had “sent English around the globe” and “a language on which the sun never sets” [1] ... As he puts it “language exits only in the brains and mouths and ears and hands and eyes of its users When they succeed, on the international stage, their language succeeds When they fail, their ... language and also the language behind the US dollar is English [1] Furthermore, education systems in English speaking countries are developed, each year hundreds of thousands students apply for education...

Ngày tải lên: 12/02/2014, 20:20

... 57.95 86. 01 46. 21 50 1. 29 55 569 7.0 (40.2) 15 .2 (2.3) 93.9 (70.4) 10 1. 29 15 .5 19 .3 17 7 (14 3.6) 58 .12 85. 91 46 .10 50 1. 16 73 638 7.2 (35.7) 20 .1 (2 .1) 91. 8 (58.9) 10 1. 16 15 .4 18 .8 242 (2 21. 5) 57.50 ... 58 .11 85.97 46.42 50 1. 02 11 3 227 5.7 (38.2) 15 .3 (2.6) 95.8 (65.0) 10 1. 02 12 .4 14 .2 292 (207.3) 57.77 86 .13 46.28 50 1. 20 66 626 8 .1 (44.2) 11 .3 (2.5) 91. 6 (62.7) 10 1. 20 16 .4 20 .1 1 51 (12 9.8) ... 50 1. 50 37 010 6.0 (46.2) 16 .8 (2.4) 99.7 (99.2) 10 1. 50 14 .9 19 .7 15 2 (12 8.5) 59. 51 86.88 45.44 50 1. 85 18 13 8 9.7 (34.5) 15 .8 (5.8) 93.2 (76.6) 10 1. 85 19 .9 23.6 84 (84) 57.94 85. 91 46 .10 50 1. 35...

Ngày tải lên: 18/02/2014, 04:20

... include skin lesions, vascular and cardiac problems, and cancer of the bladder, lungs, or skin, liver and kidney damage, damage to the nervous system, suppression of the immune system, and birth defects ... contaminated water and/ or other vectors.” The most common diseases spread through water are diarrheal diseases; examples include cholera, typhoid, paratyphoid, salmonella, giardiasis, and cryptosporidiosis ... neurological disorders Fluoride: Fluoride is a natural occurring chemical found in foods and water Fluoride is an essential element due to its importance in bone and tooth structure and growth However,...

Ngày tải lên: 18/02/2014, 04:20