1 nonseminomatous and seminomatous germ cell tumors nsgct and sgct

... alkaline phosphatase and CD30 (Figure 1c) The diagnosis was of an International Germ Cell Cancer Collaborative Group (IGCCCG) poor prognosis metastatic nonseminomatous germ cell tumor Four cycles ... urethra is also uncommon and is mainly a result of local spread from the surrounding organs Germ cell tumors of the testis are the most common solid tumors in men aged between 20 and 35 years We are ... nonpulmonary visceral metastasis (urethra and bone) indicates a poor prognosis within the IGCCCG classification [3] The spread of nonseminomatous germ cell tumors may be local, via the lymphatics...

Ngày tải lên: 11/08/2014, 03:20

... CM and cell lysates (CM and L, respectively) were collected and TSP1 was detected by western blotting Note the low TSP1 secretion and higher intracellular levels in RCC cells (F) HNK and P769 cells ... Fig Calcium uptake and mediators in HNK and RCC cells (A) Ca2+ uptake in response to VEGF by HNK and RCC cells HNK and RCC cells were preloaded with fluo-4 acetoxymethyl ester and treated with 10 ... angiogenesis, and localization in renal cells and tissue (A) Sections of RCC tumors and adjacent normal tissue (HNK) were stained for TSP1 and counterstained with hematoxylin (B, C) CM from HNK and P769...

Ngày tải lên: 07/03/2014, 05:20

... functional and structural characterization of ETB and similar GPCRs Further analysis of the identified ETB131 and ETB93 fragments will help to identify residues involved in ligand binding and dimerization, ... bacterial, yeast or insect cells is still complicated and often inefficient [5–11] Continuous exchange cell- free (CECF) expression systems based on Escherichia coli cell extracts have recently ... renal function, and regulation of cell proliferation, and it thus plays an important role in physiopathologic disorders such as congestive heart failure, diabetes, atherosclerosis, and primary pulmonary...

Ngày tải lên: 16/03/2014, 10:20

... Features Assay type Cell types Cells enter or cross cellulose membrane Cells move toward and enter capillary filled with attractant Cells avoid capillary filled with control buffer Cells migrate in ... glass and slide across a linear bridge separating chemoattractant and buffer reservoirs; cells adhere to cover glass during migration Cells migrate between cover glass and slide; cells and chemoattractant ... trajectories, velocities, and direction changes of individual cells (together referred to as “tracking” assays) as well as determinations of cell orientation, cell cell interactions, cell morphology,...

Ngày tải lên: 11/04/2014, 09:43

... the proliferation of NB4-R2 cells and induces cell apoptosis NB4-R2 cells were incubated with increasing doses of VX-680 (1, 2, and 10 nM) for 24 hr and 48 hr (A) Cell viability was measured ... depolarization and cellular caspase activation in NB4-R2 cells (A) VX-680 treated NB4-R2 cells were stained with JC-1 probe and measured by flow cytometry X- and Y-axes were indicative of monomer and aggregates, ... (Figure 3A) and caused formation of monopolar structures in NB4-R2 cells (Figure 3B) In both dose- and time-dependent manners, VX-680 suppressed NB4-R2 cells growth (Figure 4A) and induced cells apoptosis...

Ngày tải lên: 18/06/2014, 19:20

... grade Material and methods Cell lines and human oesophageal specimens This study used three human oesophageal squamous cell carcinoma cell lines and an oesophageal adenocarcinoma cell line Moderate-differentiated ... tumour tissues and oesophageal squamous cell carcinoma cancer cell lines, and is related to clinical pathology and prognosis of the patients with oesophageal squamous cell carcinoma Cellular function ... USA) and air-dried Following rehydration, cells were seeded at a density of 30,000 per insert and allowed to invade for days After incubation, cells that had migrated through the matrix and adhered...

Ngày tải lên: 18/06/2014, 19:20

... per cell (PFU/ cell) Cells were cultured in a maintenance medium (containing 2% FCS) and were left for days to allow for the plaques and the cell fusion to develop Photographs of the infected cells ... in 4T1 cells than in Vero cells, and final titers in 4T1 cells were more than two logs lower for HSV-1(F) and OncSyn, while OncdSyn viral titers were more than three logs lower on 4T1 cells than ... OncSyn and OncdSyn grown HSVComparative kinetics of viral replication of wild-type HSV-1(F) and mutant viruses OncSyn and OncdSyn grown on Vero and 4T1 cells Near confluent monolayers of Vero (a) and...

Ngày tải lên: 20/06/2014, 01:20

... the proliferation of NB4-R2 cells and induces cell apoptosis NB4-R2 cells were incubated with increasing doses of VX-680 (1, 2, and 10 nM) for 24 hr and 48 hr (A) Cell viability was measured ... depolarization and cellular caspase activation in NB4-R2 cells (A) VX-680 treated NB4-R2 cells were stained with JC-1 probe and measured by flow cytometry X- and Y-axes were indicative of monomer and aggregates, ... (Figure 3A) and caused formation of monopolar structures in NB4-R2 cells (Figure 3B) In both dose- and time-dependent manners, VX-680 suppressed NB4-R2 cells growth (Figure 4A) and induced cells apoptosis...

Ngày tải lên: 20/06/2014, 03:20

... grade Material and methods Cell lines and human oesophageal specimens This study used three human oesophageal squamous cell carcinoma cell lines and an oesophageal adenocarcinoma cell line Moderate-differentiated ... tumour tissues and oesophageal squamous cell carcinoma cancer cell lines, and is related to clinical pathology and prognosis of the patients with oesophageal squamous cell carcinoma Cellular function ... USA) and air-dried Following rehydration, cells were seeded at a density of 30,000 per insert and allowed to invade for days After incubation, cells that had migrated through the matrix and adhered...

Ngày tải lên: 20/06/2014, 04:20

... 2c and 2f, CD68 and Flt-1 co-localized in monocyte-like cells (arrows) and MNCs (arrowheads) in these specimens These results suggest that MNCs and monocyte-like cells (thought to be OCs and ... in giant cell tumors (GCTs) of bone Surgical specimens were fixed in formalin and serially sectioned (a) VEGF was expressed in stromal cells (arrows), monocyte-like cells (arrowheads) and multinuclear ... Co-localization of CD68 and Flt-1 in GCTs The sections were prepared as described in Fig and stained with anti-CD68 (a and d) and anti-Flt-1 (b and e) Abs, followed by TRITC- and FITC-conjugated...

Ngày tải lên: 20/06/2014, 04:20

... 2c and 2f, CD68 and Flt-1 co-localized in monocyte-like cells (arrows) and MNCs (arrowheads) in these specimens These results suggest that MNCs and monocyte-like cells (thought to be OCs and ... in giant cell tumors (GCTs) of bone Surgical specimens were fixed in formalin and serially sectioned (a) VEGF was expressed in stromal cells (arrows), monocyte-like cells (arrowheads) and multinuclear ... Co-localization of CD68 and Flt-1 in GCTs The sections were prepared as described in Fig and stained with anti-CD68 (a and d) and anti-Flt-1 (b and e) Abs, followed by TRITC- and FITC-conjugated...

Ngày tải lên: 20/06/2014, 07:20

... seminomas and Sertoli cell tumors filled the lumens or sheets of the affected seminiferous tubules These cells replaced the normal lining of spermatogenic and Sertoli cells Large and irregular cellular ... and canine Canine seminomas and Sertoli cell tumors testicular tumors In the current seminoma series, inhibin-alpha was observed in the cytoplasm of tumors cell in 45% of the cases Vimentin and ... male germ cell tumors Anticancer Res 2007, 27, 1685-1688 Owston MA, Ramos-Vara JA Histologic and immunohistochemical characterization of a testicular mixed germ cell sex cord-stromal tumor and...

Ngày tải lên: 07/08/2014, 23:22

... 4.0% for renal cell carcinoma, 0.84% to 3.6% for transitional cell carcinoma, 0.4% for germ cell tumors, and 0.36% to 0.7% for prostate adenocarcinoma[9] Testicular mixed germ cell tumors are common, ... roughly 33% of all testicular tumors The reason for such a high incidence of testicular mixed germ- cell tumors is because germ cells in the testes are totipotent and can undergo either trophoblast ... case reports and review of literature J Cutan Pathol 2009, 37(4):486-90 16 International Germ Cell Consensus Classification: A prognostic factor based staging system for metastatic germ cell cancers...

Ngày tải lên: 09/08/2014, 03:21

... giant cell tumors occurring outside the extremities in combination with a short review of the literature Patients and Methods Between 2000 and 2006 a total of five patients with giant cell tumors ... appearance of malignant giant cell tumors of bone or malignant foci inside benign giant cell tumors has been described also in a small number of patients [24,25], and pulmonary metastases can ... acquisition, literature review and drafted the manuscript CTI, FZ and CTH participated in data acquisition and literature review MB, JD and PEH participated in drafting the manuscript and revised it critically...

Ngày tải lên: 09/08/2014, 08:22

... Bcl-XL, and Mcl-1) Bcl-2 was over-expressed in all fresh cases, and cell lines except the WSU-WM (expressed low levels), Bcl-XL was expressed in all patient cells and cell lines (except WSU-ALL cell ... throughout the experiment by cell count and viability every 24 hr for 72 hr using 0.4% trypan blue stain (Gibco BRL, Grand Island, NY) and a hemacytometer Fresh primary lymphoma cells isolated from patients ... IRB-approved protocol and used to assess the TW-37 cytotoxic effect on primary lymphoma cells The CLL/SLL cells expressed CD5, CD19, CD20 and faint monotypic SIg The MZL cells were CD5-, CD19+ and CD20+...

Ngày tải lên: 10/08/2014, 22:20

... cytometric analyses to look at cell cycle progression Finally, the transcript levels of the genes involved in cell division and cell differentiation were tested in wild type and BR mutants Our results ... of cells in the three phases of the cell cycle–G1, S, and G2/M–were evaluated by the Partec software When cell division indices (ratio of G2M% vs G1%) were compared, the portion of wild-type cells ... percentage of cells in the three phases of the cell cycle–G1, S, and G2/M–were evaluated with the Partec software The results are summarized in Figure When Ws-2 wild-type and dwf7-1 cells were compared,...

Ngày tải lên: 11/08/2014, 11:21

... characteristics It is also mandatory to execute a lymphadenectomy in case lymph nodes are invaded Maiorano E, Altini M, Favia G: Clear cell tumors salivary glands, jaws and oral mucosa Semin Diagn ... Histological view Histological view 10× H&E – proliferation of uniform and monomorphic cells with small nuclei and clear cytoplasm; no atypia and no mitosis Page of (page number not for citation purposes) ... Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA) for his efforts and support in the final histological analysis References In the maxillo-facial area, clear cell odontogenic...

Ngày tải lên: 11/08/2014, 21:22

... BiFC signals and CT-B staining in live cells (C) Gag-VN/IMP1-VC BiFC signals and CT-B staining in live cells (D) HeLa cells or (E) Jurkat T cells were co-transfected with pCMV-Rev and Rev-dependent ... associations [13,58,59], vRNA encapsidation [49] and Gag trafficking Methods Cell culture and transfections HeLa cells and Jurkat T cells were maintained under standard conditions at 37°C with in Dulbecco's ... min) The cells were then fixed, stained for Caveolin-1 and Gag and visualized by laser scanning confocal microscopy (C) Hela cells were co-transfected with pCMV-Rev and either Gag-VN and Staufen1-VC...

Ngày tải lên: 12/08/2014, 23:23

... reported that tetherin impairs cell- to -cell transmission of HIV-1 in several cell lines and primary CD4 + T cells, and transmission of Vpudefective HIV-1 to target CD4+ T cells is less efficient than ... [25] and tetherin (BST-2 or CD317) [26,27] Tetherin is a host transmembrane protein [26,27] and is expressed by a wide-range of human and animal cells [28,29] Mouse and human pDCs [30,31] and ... donors were treated with IFNa and LPS and analyzed for tetherin expression on the surface and in whole cell lysates by flow cytometry and immunoblotting, respectively Cell surface tetherin in iDCs...

Ngày tải lên: 13/08/2014, 01:20

... production of T cells, B cells and dendritic cells Formation of structured primary and secondary lymphoid organs was seen with human cells engrafting in thymus, bone marrow, spleen and lymph nodes ... post-infection and cells were stained with CD3 and CD4 antibodies and FACS analyzed To determine the levels of CD4 T cells in the whole T cell population (stained with the pan T cell marker CD3), ... marker CD45 and FACS analyzed to verify engraftment CD34 cells and cell culture Human fetal liver-derived CD34 cells were purified and cultured in cytokine media containing IL-3, IL-4 and SCF as...

Ngày tải lên: 13/08/2014, 09:20