1 cellular origin of hrs and lp cells

... indicated times Cells in the absence of any of these were taken as the control (Con) Two micrograms of total RNA were reverse transcribed with random primers and the levels of PEPCK and G6Pase mRNA ... Each band was analysed densitometrically and the values are depicted after normalization of PEPCK and G6Pase bands with those of 18S rRNA (B) Each point is the mean ± SEM of three sets of experiments ... primary cells and animal models but, as a preliminary step, they unravel one of the mechanisms of TNFamediated withdrawal of insulin action in HepG2 cells Results Incubation of HepG2 cells with...

Ngày tải lên: 16/03/2014, 02:20

... CCR1, CCR2 and CCR5 mRNA expressing Quantification of CCR1, CCR2 and CCR5 mRNA expressing cells in defined lesional stages Mean numbers of CCR1+ cells (A), CCR2+ cells (B) and CCR5+ cells (C) ... results of the present study and from previous ones on the role of CCR1 and its ligand CCL3 in the pathogenesis of MS [39,40,42] and EAE [22,44,46], have provided evidence for an important role of ... associated with distinct expression of CCR1, CCR2, and CCR5 by cells of the macrophage/microglia lineage within the CNS lesions CCR1 and its ligands CCL3, CCL5 and CCL7 have previously been shown...

Ngày tải lên: 19/06/2014, 22:20

... degenerated disc cells, the structure and function of the degenerated cells changed remarkably Alini et al[11] observed an increase and maintain in density of bovine NP cells and synthesis Page of 11 (page ... effect of growth factors on proliferation of human NP cells (10%FBS/F12) (n = 20) Figure Dose-response effect of growth factors on proliferation of human NP cells (10%FBS/F12) (n = 20) Cells were ... Dose-response effect of growth factors on proliferation of human NP cells (1%FBS/F12) (n = 20) Dose-response effect of growth factors on proliferation of human NP cells (1%FBS/F12) (n = 20) Cells were...

Ngày tải lên: 20/06/2014, 00:20

... long-term and physiological marking, the possibility of determining the generation of single cells from the amount of label within the cell, and the possibility of labeling both donor and recipient cells ... Qualitative co-localization of DNA and RNA through simultaneous imaging of RNA and DNA Rat embryo fibroblasts were pulsed with 15N-uridine and BrdU as markers of newly synthesized RNA and DNA, respectively ... values of 12C14N- counts, 12C- counts and of the 12C14N-/12C- ratio are shown in Figure 10f for a group of pixels constituting the central dot of the inset in Figure 10e, and the values of the...

Ngày tải lên: 06/08/2014, 18:21

... telegraphic with omissions of function Table 1: Performance of FG and norms (mean and standard deviation or range) on neuropsychological and language tests Test Working memory and executive functions ... shown consistent alteration of language and cognitive functioning in clinical populations [39] Because of the acute onset and stability of the symptoms in FG, the presence of a neurodegenerative process ... coordinator SP and JM reviewed the existing literature and drafted the manuscript MF and NP reviewed the manuscript and contributed to the writing NP and LG conducted the brain imaging exams and interpreted...

Ngày tải lên: 08/08/2014, 21:20

... representative of three individual wells Page of The secretion of BDNF in HepG2 and HCCLM3 cells by ELISA BDNF is a cytokine secreted by a few human cancers, supporting growth and survival of tumor cells ... anti-BDNF treated cells, which indicated that BDNF was required for supporting the survival of HepG2 and HCCLM3 cells The involvement of BDNF in the invasion of HepG2 and HCCLM3 cells was also confirmed ... BDNF/TrkB supports the survival of HCC cells, and seems to serve as a critical mediator in the progression of intrahepatic dissemination of HCC cells, and prevention of BDNF/TrkB signaling could...

Ngày tải lên: 10/08/2014, 10:21

... projection of the surface of the distal area of the stigma at stage C: stacks projection of the surface of the central area of the stigma at stage Note the differences in both the texture of the ... distribution of the labelling by TMB and DCFH2-DA), and the relative low presence of other ROS and NO in these stages (as showed by the DHE and DAF-2 DA fluorophores) (Figure 9) The average level of DCFH2DA ... purpose of this study was to first approach the possible implications of ROS and NO during flower Page of 14 development and the pollen-pistil interactions in the olive For this purpose, several of...

Ngày tải lên: 12/08/2014, 03:21

... of immune cells including CD4+ T cells, CD8+ T cells, and gδ-T cells, as well as DN T cells and NK cells [5,10,11,13] Of interest, Crispin et al [5] showed expansion of IL-17-secreting DN T cells ... evidence of a role for IL-17 in the pathogenesis of SLE, with the demonstration of an increased frequency of Th17 cells in the peripheral blood of lupus patients, and a correlation of the frequency of ... frequency (% of CD4+IL-17+ T cells, CD4+IL-17+IFN-g+ T cells and CD4+IFN-g+ T cells in patients with SLE and in healthy control subject (HC) Numbers in dot plots indicate the frequency of cells for...

Ngày tải lên: 12/08/2014, 12:20

... proliferation of epithelial and hematopoietic cells [19]; however, TGF-β1 induces proliferation of the mesenchymal phenotype of cells such as fibroblasts, smooth muscle cells, and myofibroblasts ... concentration-dependent expression of CTGF gene and protein The TGF-β1 induced phosphorylation of Smad 2/3 and the expression of CTGF mRNA and protein were all blocked by the inhibition of ERK and JNK, but not ... muscle cells, Xie and coworkers found that TGF-β1 induced a significant activation of Smad 2/3 and translocation of phospho-Smad 2/3 and Smad from cytosol to nucleus, as well as a timeand concentration-dependent...

Ngày tải lên: 12/08/2014, 16:20

... subsets of human T cells Analysis of TLRs in T cells requires removal of contaminating cells expressing high levels of TLRs, such as monocytes, B cells and DCs FACS analysis of the purified T ... http://respiratory-research.com/content/7/1/36 CD8+ T Figure CD4 cells express higher levels of TLR3 and TLR4 than CD8+ T cells express higher levels of TLR3 and TLR4 than CD4+ T cells Freshly isolated CD4+ and CD8+ T cells from human tonsils ... analyses and interpretation of data, drafted the manuscript and was involved in the design of the study MA and LOC made substantial contributions to conception and design of the study, and were...

Ngày tải lên: 12/08/2014, 16:20

... bio-ligands Ligands and theand2 of bio-ligands and binding ATP, ligands Additionalfor file 1ownerships of metabolism-relevantADP and block numbers of for proteins folds that bindligands ligands them ... ACD-SC-derived ordinary molecules; Ligands, sc-PDB-derived ligands; Ligands (≤ 3), ligands covering ≤ folds; Ligands (4-9), ligands covering 4-9 folds; Ligands (≥ 10), ligands covering ≥ 10 folds Implications ... the origin of life Thus, it is intriguing to explore the chronology of ligand-protein binding and answer the following questions: which ligand was first recognized by a protein and what kind of...

Ngày tải lên: 14/08/2014, 08:20

... (minimum height of base of terminal) Type of roof Location not within 1.5 m of a vertical surfacea of a structure on the roof Internal route Location within 1.5 m of a vertical surfacea of a structure ... roof, the base of the outlet shall be above the level of the ridge of the roof Where the chimney or flue pipe passes through a flat roof the base of the outlet shall not be below the highest of ... thermal input of the appliance, the period of continuous use and the size of the room Reference should be made to the appropriate appliance and installation specifications, codes of practice and manufacturers’...

Ngày tải lên: 28/09/2014, 23:27

... displayed the highest standards of professional competence and conduct, but also demonstrated his scholarly demeanor viii and sincere concern and respect for the needs of his students His uncompromised ... before and after his graduation I also want to thank ix Dr Cuneyt Eroglu, another past doctoral student of the logistics program and my officemate and friend, with whom I shared most of my time and ... none of them offered a complete picture of why, when, and how to manage such a trilateral business relationship [20] From the area of supply chain management, Lambert and Cooper (2000) and Lambert,...

Ngày tải lên: 02/11/2014, 00:44

Ngày tải lên: 14/11/2014, 12:59

Ngày tải lên: 25/08/2016, 19:46

... model of a PHE, and the solution allows the calculation of the pressure and temperature change of streams entering the heat exchanger It is a problem of PHE rating (analysis) Optimization of PHE ... Wang and Sunden [10] The most important and costly parts of plate -and- frame PHE are plates with gaskets The plates can be made of stainless steels, titanium and other even more expensive alloys and ... the ratio of going through block heat capacities of streams; G2 and c2 mass flow rate [kg/s] and specific heat [J/(kg K)] of cold stream If Rb ¼ 1, then eb ¼ NTUb/(1 þ NTUb) In case of co-current...

Ngày tải lên: 26/08/2016, 18:04

... steps of the CNS inflammation process, for example migration of leukocytes to inflammatory sites [14,15] and adhesion of polymorphonuclear cells and monocytes to CNS cells [16,17] Schwann cells ... expression in Schwann cells of sciatic nerves Effects of LPS on expression of ICAM-1 in SCs in vitro In order to better explore the role of LPS-induced ICAM-1 expression in SCs, a series of experiments ... antigen-presenting cells and activate T cells in vitro in an antigen-specific and MHC-restricted manner [32], especially in the presence of cytokines Natural ligands of ICAM- 1and LFA-1 are expressed...

Ngày tải lên: 18/02/2014, 18:20

... analysis of the function of Octamer motif in the fgl2 promoter Lanes 2–5 of panel A and lanes 2–5 of panel B, NE were incubated with labelled consensus Oct-1 Lanes 6–12 of panel A and lanes 6–8 of ... amounts of pPacUSp1 (50 ng, left panel) or increasing amount of Ets-1 with 50 ng of either Sp1 or Sp3 (B) Assay of fgl2 promoter activity upon cotransfection of thresholds amount of both Sp1 and ... (10 ng, right panel) and assay of promoter activity of wild-type (pfgl2 ()1320/+9) and linker-scanning mutant and following cotransfection with 10 ng of pPacUSp1, pPacUSp3, and pPacUEts-1 Luciferase...

Ngày tải lên: 20/02/2014, 11:20

... easier handling of the cells, treatment was performed on HeLa cells instead of CHO K1 cells Cells were then incubated with of lgámL)1 Tat±GFP fusion protein or with lM ¯uorescein Tat peptide and ... translocation of the full-length Tat protein and cellular uptake of its basic domain make use of the same mechanism Both genetic and biological evidence indicates that the cellular uptake of the Tat ... speci®c as a large number of cell lines tested so far entrapped the translocating peptide Fig In¯uence of temperature on the uptake of Tat CPP and of Tat±GFP fusion protein HeLa cells were incubated...

Ngày tải lên: 21/02/2014, 03:20

... 29.2 Figure Crystallogenesis of histidine-tagged isoform of EED Crystallogenesis of histidine-tagged isoform of EED (A), Platelet-like crystals of EED3-H6 protein of 441 residues, obtained in ... structure of EED Crystallogenesis of EED was therefore undertaken to better understand the nature of the multiple interactions and functions of EED in the HIV-1 life cycle Unfortunately, none of our ... the EED3 isoform), using homology modelling by sequence alignment and homology with protein(s) of known structures, and assessment of accessible motifs and epitopes at the surface of the EED...

Ngày tải lên: 20/06/2014, 01:20