1 searching for good matches in protein sequences

... A C-terminal 4· methionine tag was introduced into the nascent chains to increase the labelling efficiency The nascent chain corresponding to the authentic preprotein comprised 101 amino acids, ... sophisticated N-terminal extensions, containing multipurpose functional information A signal peptide can be divided into three distinct domains; the N-, H-, and C-domains [5,6] The N-domain interacts with ... nascent chain; ?, unknown cross-linking adducts; *, cross-linking adducts with E coli L23; d, cross-linking adducts with E coli Ffh; s, cross-linking adducts with B subtilis Ffh; n, cross-linking adducts...

Ngày tải lên: 16/03/2014, 22:20

... inserted into a loop of another protein (host protein) The basis for the selection is the ability of the host protein to bind its natural ligand For the host protein to fold it must be able to bring ... sought, they inform our perspective on protein evolution While most multidomain proteins were probably formed by linear concatenation of individual domains, surveys of the Protein Data Bank suggest ... structure of an immunoglobulin light chain-binding domain of protein L Comparison with the IgG-binding domains of protein G Biochemistry 33, 14011–14017 Kastern, W., Sjobring, U & Bjorck, L (1992)...

Ngày tải lên: 30/03/2014, 13:20

... Eosinophil granule major basic protein EBMP × Guanine nucleotide-binding protein GBP2 × 38 Cathepsin C CTSC × 17 Interleukin-1 receptor type II IL1R2 × 11 Cyclin-dependent kinase inhibitor (p21) × CDKN1A ... genes in acute lung injury Gene Ontology Encoded protein Gene symbol NR IM Interleukin-1β IL1B × × Interleukin-6 IL6 × Tissue factor precursor TF Plasminogen activator inhibitor-1 IN Lung Lung injury ... plasminogen activator PLAT × 101 Fibrinogen α FGA × 22 27 × 35 × 22 CCAAT/enhancer binding protein beta CEBPB Proteinase activated receptor PAR-2 Plasma prekallikrein Cell chemokine (MCP-1) Interleukin-8...

Ngày tải lên: 12/08/2014, 20:20

... by observing, folding, measuring and inducing - The instructional materials greatly assisted the lessons, and supported students in exploring and discovering new information - Low-cost instructional ... as a means to innovation In the lesson study teachers played a central role in deciding on the innovation in teaching and learning They were the ones to implement the innovations in their actual ... leave much room for creative thinking The idea of using open-ended problems to improve school mathematics teaching, to develop and foster methods for teaching problem-solving and thinking skills,...

Ngày tải lên: 13/08/2015, 10:21

... Many factors form (or block formation of) protein complexes that contact the RNA polymerase directly, increasing or decreasing its affinity for binding to a gene’s promoter and initiating transcription ... by RNA polymerase In Figure A, the repressor protein (R) binds to the regular binding site and blocks transcription In Figure B, the repressor protein can not bind to the binding site due to some ... matching of bases by finding an optimal alignment 13 CHAPTER BACKGROUND Sequence similarity forms the basis to find interesting features in long genomic sequences Similar substrings between sequences...

Ngày tải lên: 03/10/2015, 21:58

... improvement in clinical indicators of pain and disease activity A first randomized controlled trial in patients with knee OA demonstrated a good safety profile for one intra-articular injection ... performed β in osteoarthritis with IL-1β blockers? It is indeed tempting to compare IVD degeneration to OA because the profile of cytokine production in human IVDs looks like the one observed in ... We performed a multicenter, randomized, double-blind, placebo-controlled study to evaluate the clinical response, safety, and tolerability of a single intra-articular injection of anakinra (50...

Ngày tải lên: 09/08/2014, 10:21

... synthesis [29] Protein Kinase C (PKC) is a class of serine/ threonine kinase that is subdivided into three main groups, namely conventional PKCs, novel PKCs, and atypical PKC [30,31] Protein kinases ... is critical for the induction of IFN-b synthesis in cDC Future work could be pursued to determine additional binding partners or signaling molecules in TLR7/9-induced IFNb pathway in CDC localization ... (2007) Protein Kinase Ca is involved in Interferon regulatory factor activation and type I Interferon-b synthesis J Biol Chem 282: 15022–15032 30 Mochly-Rosen D, Gordon AS (1998) Anchoring proteins...

Ngày tải lên: 23/09/2015, 14:47

... clusters in a training collection The task of -optimization leads to a system of linear inequalities, which we solve via linear programming As there is no scope for this issue here, we cannot go into ... Harshman 1990 Indexing by latent semantic analysis JASIS, 41(6):391–407 Inderjit S Dhillon and D S Modha 2001 Concept decompositions for large sparse text data using clustering Machine Learning, 42(1/2):143–175 ... tagging: Data vs dicc tionaries In Proceedings of the 6th ANLP Conference, 1st NAACL Meeting, pages 94–101, Seattle Martin Holub 2003 A new approach to conceptual document indexing: Building...

Ngày tải lên: 08/03/2014, 04:22

... programme taught in English Launched in April 1999, it was the first of its kind in Germany The programme covers two semesters of courses, including two months for writing and defending a Master’s ... international institutions of higher learning in Germany and enjoys a vibrant international community Another chance of getting access to an international network is provided by taking part in the annual ... the students in adjusting to life and study in Osnabrück In addition, the International Faculty Office at the Faculty of Business Management and Social Sciences (IFO) provides information, support...

Ngày tải lên: 08/03/2014, 04:22

... double-stranded RNA activated protein kinase induced by interferon Cell 62, 379–390 Hovanessian, A.G (1989) The double stranded RNA-activated protein kinase induced by interferon: dsRNA-PK J Interferon Res ... the interferoninduced double-stranded RNA-activated protein kinase Proc Natl Acad Sci USA 90, 232–236 Lee, S.B & Esteban, M (1994) The interferon-induced doublestranded RNA activated protein kinase ... & Yao, Z (1996) Molecular cloning and characterization of a novel protein kinase with a catalytic domain homologous to mitogen-activated protein kinase kinase kinase J Biol Chem 271, 31607–31611...

Ngày tải lên: 08/03/2014, 09:20

... retinoid-binding protein genes was performed using clustalx [53] A bootstrap neighbor-joining phylogenetic tree was constructed using the zebrafish intestinal-type fatty acid-binding protein sequence ... acidbinding protein family that binds all-trans-retinol J Biol Chem 276, 1353–6130 Dong D, Ruuska SE, Levinthal DJ & Noy N (1999) Distinct roles for cellular retinoic acid-binding proteins I ... from any other tissues examined including the liver, ovary, skin, intestine, heart, muscle and testis crabp1b mRNA was detected by RT-PCR in the skin, intestine, brain and muscle of adult zebrafish...

Ngày tải lên: 16/03/2014, 22:20

... connexins and innexins contain four transmembrane domains, and combine to form dodecamers [14,16–19] In contrast to ligandgated channels, the four transmembrane domains of connexin and innexin are ... surface method Protein Eng 10, 673–676 10 Sonnhammer, E.L., von Heijne, G & Krogh, A (1998) A hidden Markov model for predicting transmembrane helices in protein sequences Proc Int Conf Intell Syst ... of maximizing detection and minimizing false-positives The first goal is achieved by applying the algorithm to a sequence database containing all proteins that belong to the family of interest...

Ngày tải lên: 17/03/2014, 23:20

... pathways Because laminarin has been reported to induce ethylene-dependent PR proteins but not SA-dependent PR proteins in tobacco [18], and because the responses induced by AaGlucan in tobacco tissue ... cells, PCR was performed with denaturing, annealing and extension temperatures of 94 °C for 0.5 min, 50–58 °C for 0.5 and 72 °C for 0.5 min, respectively, for 24–34 cycles The annealing temperature ... expected, laminarin application did not induce cell death, whereas a treatment with benzyladenine resulted in staining of 76% of the BY-2 cells These results indicate that the AaGlucan does not induce...

Ngày tải lên: 23/03/2014, 11:20

... exists on the role of this protein in the biogenesis of heme-containing proteins in plants To gain insight into this process, we decided to study the role of frataxin using the enzyme catalase as ... demonstrated that frataxin serves as a potential donor to FC for insertion of iron into the protoporphyrin ring during heme synthesis Knocking down the expression of frataxin in human cells revealed ... mitochondria Indeed, the specific antibodies against either of the two isoforms of FC detected signals only in plastids [42] In Chlamydomonas reinhardtii, where a single gene encodes for FC, the protein...

Ngày tải lên: 28/03/2014, 23:20

... with intact proteins in solution Furthermore, a close inspection of the results showed differences between the apo -protein and the holo -protein Interestingly, the presence of led to a larger increase ... domain does not interact in the same way with an A domain poised for thioester formation, as if the binding interface between A and PCP domains is only created for the holo-PCP This would be in ... using catalytically competent proteins We used a tightly binding inhibitor of the A domain to achieve synchronization of the protein ensemble This strategy appears to be promising for capturing...

Ngày tải lên: 29/03/2014, 08:20

... domain are shown The protein kinase A (PKA)dependent phosphorylation sites, Ser577 in SIK1 and Ser587 in SIK2, are indicated in bold The basic residues in domain 3B are indicated by underlines ... Calmodulin kinase II attenuation of gene transcription by preventing cAMP response 17 18 19 20 21 22 23 24 25 26 27 28 29 element-binding protein (CREB) dimerization and binding of the CREB-binding protein ... vector, incubated for 16 h and stimulated with or without ACTH (10)6 M) for h, fixed with mL of 4% paraformaldehyde dissolved in NaCl/Pi for 15 min, stained with DAPI [1 ngÆmL)1 in NaCl/Pi containing...

Ngày tải lên: 30/03/2014, 15:20

... protein zfh-1 [19], a factor containing both zinc finger and homeodomain motifs It is a 124 kDa DNA-binding protein which was initially characterized as a negative regulatory factor involved in ... significant changes in transcription Interestingly, within this region, consensus sequences homologous to adaptor protein (AP1) and d-crystallin enhancer factor (dEF1) binding element were identified ... acid protein promoter alters binding of an activating protein1 complex and is associated with altered transcription and serum levels J Biol Chem 276, 32466–32473 Fortini ME, Lai ZC & Rubin GM...

Ngày tải lên: 30/03/2014, 16:20

... binding, T cell recognition or proteasomal processing Therefore, mutation analysis was performed in both samples obtained before vaccination and during progression However, no mutation leading ... mechanism in this single patient WT1 mutations have been reported in about 10% of patients with AML [23] They were frequently observed in DNA binding portions of the WT1 protein and may therefore ... Medicine 2010, 8:5 http://www.translational-medicine.com/content/8/1/5 within a phase II vaccination trial and progressed after an initial interval of vaccine efficacy [8] T cell response to vaccination...

Ngày tải lên: 18/06/2014, 16:20

... organizing the initial training course for master trainers, following-up on activities after this training (including regional planning meetings), and advising on the selection of four Regional Training ... Cascade1training Cascade training NTP: National Tuberculosis Control Programme NTC: National training coordinator PTC: Provincial training coordinator HRD: Human resource development TOT: Training of ... management in pre-service training curricula The plan included activities to initiate the process to update curricula in national and private training institutions, to be in line with national guidelines...

Ngày tải lên: 18/06/2014, 17:20

... selecting only moving objects of interest in the scene The adaptive threshold is obtained by using δn, the recursive average of Δn, as a variable amplifying gain for the threshold (17) The increase ... segmentation is performed on gray level images resulting in binary images containing “moving” and “static” pixels Each image is then divided in blocks of 10 × 10 pixels If a block contains more than ... improved in [7] where its inherent trailing effect is compensated by a confidence weight representing the confidence of a pixel being part of the foreground Adaptive threshold for motion detection in...

Ngày tải lên: 21/06/2014, 07:20