fi nity by surface plasmon resonance

... single binding events by localized surface plasmon resonance Nano Lett 2008, 8:3450-3455 11 Scarano S, Mascini M, Turner AP, Minunni M: Surface plasmon resonance imaging for affinity-based biosensors ... of guanine-guanine mismatches in DNA by synthetic ligands using surface plasmon resonance Nat Biotechnol 2001, 19:51-55 Chu LQ, Forch R, Knoll W: Surface- plasmon- enhanced fluorescence spectroscopy ... for the microorganism analysis List of abbreviations SPR: Surface plasmon resonance Acknowledgements This study was supported in part by grants from the National Natural Science Foundation of...

Ngày tải lên: 18/06/2014, 19:20

... single binding events by localized surface plasmon resonance Nano Lett 2008, 8:3450-3455 11 Scarano S, Mascini M, Turner AP, Minunni M: Surface plasmon resonance imaging for affinity-based biosensors ... of guanine-guanine mismatches in DNA by synthetic ligands using surface plasmon resonance Nat Biotechnol 2001, 19:51-55 Chu LQ, Forch R, Knoll W: Surface- plasmon- enhanced fluorescence spectroscopy ... for the microorganism analysis List of abbreviations SPR: Surface plasmon resonance Acknowledgements This study was supported in part by grants from the National Natural Science Foundation of...

Ngày tải lên: 20/06/2014, 03:20

... 250 250 The current study confirms and extends our initial observation using af nity CE [14] by applying two additional biophysical techniques to confirm the specificity of this interaction with ... experiments were homogeneous as verified by mass spectrometry (data not shown) The successful immobilization of AP peptides was confirmed by the observation of a 2500 resonance unit (RU) increase in ... continuously flowed over the sensor surface to monitor dissociation At the end of each run, the sensor surface was regenerated by injecting a 10-lL pulse of M NaCl, followed by reinstituting flow with sodium...

Ngày tải lên: 31/03/2014, 23:20

... Hutter E, Fendler JH: Exploitation of localized surface plasmon resonance Adv Mater 2004, 16:1685 13 Willets KA, Van Duyne RP: Localized surface plasmon resonance spectroscopy and sensing Annu Res ... surface plasmon resonance spectroscopy of triangular silver nanoparticles J Am Chem Soc 2002, 124:10596 17 Hiep HM, Yoshikawa H, Saito M, Tamiya E: An interference localized surface plasmon resonance ... Biosensing with plasmonic nanosensors Nat Mater 2008, 7:442 15 Xiang G, Zhang N, Zhou X: Localized surface plasmon resonance biosensing with large area of gold nanoholes fabricated by nanosphere...

Ngày tải lên: 21/06/2014, 04:20

... Exploitation of localized surface plasmon resonance Adv Mater 2004, 16:1685 52 Eustis S, El-Sayed MA: Why gold nanoparticles are more precious than pretty gold: nobel metal surface plasmon resonance and ... recombination rate in a quantum well by resonant surface plasmon coupling Phys Rev B 2002, 66:153305 32 Okamoto K, Niki I, Scherer A, Narukawa Y, Mukai T, Kawakami Y: Surface plasmon enhanced spontaneous ... LED by mediating surface plasmon polaritons Proc SPIE 2008, 7032:70320R 24 Gifford DK, Hall DG: Emission through one of two metal electrodes of an organic light-emitting diode via surface- plasmon...

Ngày tải lên: 21/06/2014, 05:20

... membrane af nity of the intact protein is about 10-fold higher than the af nity for Gla–EGFN and Gla–EGFNC and about 100-fold higher than the af nity to the isolated Gla domain No significant difference ... under similar conditions by different methods varied by as much as two orders of magnitude [12,13,17] We therefore decided to investigate membrane binding by surface plasmon resonance With this method ... Davies, M.C & Forsen, S., (2000) Characterization of the surface generated by liposome binding to the modified dextran matrix of a surface plasmon resonance sensor chip An Biochem 280, 29–35 Abbott,...

Ngày tải lên: 08/03/2014, 23:20

... screening speed for nding new ligands, especially at the primary screening step Recently, the surface plasmon resonance (SPR) biosensor technology has been recognized as a powerful tool in monitoring ... with desired concentration was concentrated through a Centricon concentrator on demand Surface plasmon resonance (SPR) analyses The interaction analyses between immobilized PPARc LBD and its ... processed by using automatic correction for nonspecic bulk refractive index effects All the equilibrium constants (KDs) evaluating the proteinligand binding afnity were determined by the steady...

Ngày tải lên: 16/03/2014, 16:20

... light-emitting diode with a localized surface plasmon resonance Microelectron Eng 2009, 86:1120-1123 Lepage D, Dubowski JJ: Surface plasmon effects induced by uncollimated emission of semiconductor ... Burke JJ, Stegeman GI, Tamir T: Surface- polariton-like waves guided by thin, lossy metal films Phys Rev B 1986, 33:5186 Mills DL: Attenuation of surface polaritons by surface roughness Phys Rev B ... Lepage et al.: Plasmonic propagations distances for interferometric surface plasmon resonance biosensing Nanoscale Research Letters 2011 6:388 Submit your manuscript to a journal and benefit from: Convenient...

Ngày tải lên: 21/06/2014, 03:20

... wet-etched surface, with the relationship of 0 xh À xh 2  2  2 0 þ yh À yh þ zh À zh ¼ te2 P0 is also on the normal to the surface expressed by Eq Based on Eqs and 8, P0 can be calculated by ,sffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffi ... scattering due to the rough glass surface caused by unspecific dry etching, and can form nanoparticle clusters that might further enhance the electromagnetic field of the nanostructures Acknowledgments ... cannot be observed by scanning electron microscope (SEM) or atomic force microscope (AFM), the profiles of the nanostructures at different etching intervals are also simulated, and we find that the trend...

Ngày tải lên: 22/06/2014, 00:20

... ahead of CPT Surface plasmon resonance (SPR) biosensing is an analytical technique that requires neither radiochemical nor fluorescent labels to provide real-time data on the affinity, specificity, ... inhibitor, Figure Surface plasmon resonance sensorgram for the immobilized recombinant human topoisomerase I (hTopI) (A) A sensorgram of hTopI immobilized on the General Layer Medium sensor surface (B) ... JL, Hsu SM, Lin S: Determination of binding constant of DNA-binding drug to target DNA by surface plasmon resonance biosensor technology Curr Drug Targets Immune Endocr Metabol Disord 2005, 5:61-72...

Ngày tải lên: 10/08/2014, 05:21

... frequency known as the resonance frequency The resonance frequency changes as the thickness of the surface of the crystal changes As the mass of the surface changes, the resonance frequency also ... in affinity columns The beads facilitate selection by incubating with the target in solution Researchers can then remove the binding sequences by magnet or spin columns Another affinity surface ... the ligand on the surface of the sensor and causing a boost in signal Another mass sensitive biosensor which is of increasing interest in the life sciences is surface plasmon resonance (SPR) Researchers...

Ngày tải lên: 10/09/2015, 09:22

... applications by incorporating plasmonic resonance tuning 1.3.4 Tuning of surface plasmon resonance by bimetallic structures To the best of our knowledge, there has yet being any research on the surface plasmonic ... particle This resonance is called the localized surface plasmon or short localized plasmon resonance [7] Another consequence of the curved surface is that plasmon resonances can be excited by direct ... Background 2.1 Fundamentals of surface plasmons (SPs) 2.1.1 Basic properties of surface plasmons Surface plasmon polaritons (SPPs), often referred to as surface plasmons (SPs), are resonant electromagnetic...

Ngày tải lên: 16/10/2015, 11:57

... book edited by Petit [13] incident light diffracted modes surface plasmon Fig 2.3 Sketch of the diffraction induced by a grating surface 2.1.2.3 Factors affecting surface plasmon resonance So ... polymethylmethacrylate PSP: plasmon surface polariton QCM: quartz crystal microbalance rpm: revolutions per minute SPANI: sulfonated polyaniline SPS: Surface Plasmon Spectroscopy SPR: surface plasmon resonance ... Techniques……………………………………………29 3.1.1 Surface plasmon spectroscopy…………………………………………….29 3.1.2 Electrochemical -surface plasmon spectroscopy……………………… 32 3.1.3 High pressure surface plasmon spectroscopy with...

Ngày tải lên: 08/11/2015, 17:00

... originated by an LSP excitation in the wires, as it was pointed out in [10,11], being its spectral position related to the variation of the plasmon resonance condition introduced by the modification ... localized surface plasmon resonance of noble metal nanoparticles J Phys Chem B 2004, 108:6961 Lee KS, El-Sayed MA: Gold and silver nanoparticles in sensing and imaging: sensitivity of plasmon response ... illuminated by total internal reflection Phys Rev B 1999, 59:5936 Safarov VI, Kosobukin VA, Hermann C, Lampel G, Peretti J, Marliere C: Magneto-optical effects enhanced by surface plasmons in...

Ngày tải lên: 21/06/2014, 03:20

... improve the crystallinity, the ZnMgO films were annealed in vacuum at 800 °C for h Finally, the Pt NPs were grown on the ZnMgO surface by sputtering deposition of Pt thin films followed by annealing Annealing ... observed for the ZnO film, indicating that the ZnO thin film was grown along a caxis orientation of the sapphire substrate [11] The ZnMgO film exhibits a similar XRD pattern as the ZnO film, inferring ... the thermal expansion coefficient between the substrates and Pt films, when the initial thickness of Pt films is in the scale of nanometers, the compressive stress induced by annealing Nanoscale Res...

Ngày tải lên: 21/06/2014, 20:20

... Furthermore, the principal hyperfine values of both protons change significantly upon altering the pH Thus, replacement of His354 by alanine leads to significant modification of the cofactorbinding ... which is directly observable by time-resolved EPR [2,79] The specific amino acid involved in the photoreduction of FAD in Escherichia coli CPD photolyase was first identified by a comprehensive point-mutational ... magnetic field-dependent nuclear Larmor frequency, mN, and separated by the (orientationdependent) hyperfine coupling constant A (for the case vN > jA=2j); or (b) centered around A ⁄ and separated by...

Ngày tải lên: 16/03/2014, 02:20

... reported by Millington et al [22], resulting in only a modest improvement of fit as judged from v2 values; the values were reduced by approximately 4% or less by the three-exponential fitting Next, ... accurately and finely determined by measuring the reduced fluorescence lifetime of donor proteins in the presence of acceptors Because fluorescence lifetime is, in principle, unaffected by changes in ... space-correlated single photon counting data by fitting data to a single exponential decay In the FLIM map, color corresponds to the fluorescence lifetime indicated by a false color scale (C–F) CHO-K1 cells...

Ngày tải lên: 16/03/2014, 05:20

... compared with the surface plasmon wave along the surface of the bulk metal in a dielectric medium This symmetric surface plasmon wave mode is also called the long range surface plasmon (LRSP) mode ... about these results 10 Chapter Basics of Surface Plasmon Polaritons This chapter presents some principles of surface waves, especially long range surface plasmon polaritons and excitation methods ... and the electric field E, defined via the conductivity σ by J=σE (1.7) 1.2 Surface Plasmon Polaritons at Metal-Insulator Interfaces Surface plasmon polaritons are electromagnetic excitations propagating...

Ngày tải lên: 11/04/2014, 13:49

... mechanism of reducing the near-field confinement is different from diffraction in far-field collimation For farfield collimation, the relative phase difference of waves produced by each groove is 2␲ to ... less confinement in the field Figure also shows that at distances 60–80 nm, the grooves are most effective and reduce the FWHM by 35% At a distance smaller than 20 nm, the field produced by the bowtie ... 100-nm-thick aluminum film on a quartz substrate is illuminated by a TMpolarized 400-nm-wavelength plane wave Since the surface grooves are designed to concentrate light in the near-field, the positions...

Ngày tải lên: 06/05/2014, 08:53

... “Approximate model for surface- plasmon generation at slit apertures,” J Opt Soc Am A 23(7), 1608–1615 (2006) 16 L Zhang, A Kubo, L Wang, H Petek, and T Seideman, “Imaging of surface plasmon polariton ... incident light and surface plasmon polariton (SPP) launched by the nanoslit This result will clarify the hybridization of optical fields in s-NSOM measurements, especially when studying plasmonic structures ... of graphene plasmons revealed by infrared nano-imaging,” Nature 487(7405), 82–85 (2012) 21 A Huber, N Ocelic, D Kazantsev, and R Hillenbrand, “Near-Field Imaging of Mid-Infrared Surface Phonon...

Ngày tải lên: 06/05/2014, 08:54