fi cation of differentially expressed proteins by maldi ms

... constitutive isoforms of HSP70 are synthesized in the gills and mantle of C gigas [26] The expression level of the constitutive forms increases after thermal stress, whereas the inducible one is expressed ... recovery of cells after protein damage, by assisting the folding of cytoskeletal proteins that are actively synthesized and ⁄ or renatured under these conditions The upregulation of all of these ... of these chaperones confirms the severity of the thermal stress under our experimental conditions A number of genes encoding structural components of the cytoskeleton and proteins involved in contractile...

Ngày tải lên: 18/02/2014, 16:20

... sensitivity of the original search strategy for membrane proteins that are related to AChRs only by function Candidate LGICs retrieved by the search strategy Fig Hydropathy profiles of four proteins ... characteristic pattern of peaks in their hydropathy profiles (A) The hydropathy profile of a rat dopamine symporter reveals a pair of peaks followed by a deep valley, then a cluster of nine peaks The ... and the resulting profile is filtered to reduce noise We chose a set of hydropathy values and a filter width that are near-optimal for detection of transmembrane regions [6] The filter function is a...

Ngày tải lên: 17/03/2014, 23:20

... to identify differentially expressed genes Effect of gene-specific knockdown on the accumulation of BaMV To investigate the roles of the differentially expressed genes identified by cDNA-AFLP ... expression of differentially expressed genes obtained by cDNA-AFLP This study Page of 12 examines and discusses the effects of gene-specific knockdowns on BaMV infection Results Screening of BaMV ... innate immunity by studying plants that transiently or permanently over-express FNR, to evaluate the effects of higher levels of host FNR on the replication of BaMV Lower levels of accumulated...

Ngày tải lên: 11/08/2014, 11:21

... dynamic properties of proteins in the PGC determined by FCS and FRAP A fusion protein consisting of Olvas and green uorescent protein (GFP) (OlvasGFP) expressed in the PGC forms granules that exhibit ... min Fig Time-lapse LSM analysis of OlvasGFP reveals the dynamic nature of the nuage Schematic diagram of the preparation of PGCs of medaka specimen (A) OlvasGFP was expressed in the medaka PGC at ... (FRAP) are often used to assess the dynamics and kinetic properties of proteins in living cells [1219] FCS detects the uctuations of uorescent intensity derived from the movement of a single...

Ngày tải lên: 18/02/2014, 16:20

... Design of native-like proteins The major difficulty encountered in protein design has been that designed proteins often have more heterogeneous structures than those of typical natural proteins ... Selection of folded proteins by phage-display (Eur J Biochem 271) 1611 Fig Effect of structural change on the selection (A) Structure of cyt b562 Residues M7 and H102 are the ligands of heme Heme ... loop of the native structure, they could prevent the selection of stable proteins By examining the structures of the proteins studied by the phage-display and proteolysis, we found that protease...

Ngày tải lên: 19/02/2014, 12:20

... chosen concentrations of reporter DNA, PKG induces a less than twofold induction of transactivation by endogenous p65) Expression of the transfected proteins was confirmed by Western blotting All ... differential phosphorylation of p49 by PKG but much less by PKA, and the phosphorylation of p65 by PKA and PKG on distinct residues implies multiple modes of regulation of NF-jB function It also ... case, the lack of transactivation is not overcome by cotransfection of PKG (Fig 2B) We noted that the transfection of PKG alone was sufficient to increase the consensus reporter activity by approximately...

Ngày tải lên: 08/03/2014, 02:20

... filtration of wild-type S100A12 (WT) and its C-terminal truncation mutants Gel filtration of the purified proteins (WT, C89, C86 and C74) was carried out on a Superdex 75 column in the absence of ... for 30 at 25 °C Reactions were quenched by adding Tris to a final concentration of 50 mM Proteins were visualized by 12% Tricine/SDS/PAGE Gel filtration Gel filtration was carried out on a Superdex ... IDH were fitted to the bivalent binding model When the binding curves were fitted to the bivalent model, the Ôgoodness of fitÕ was indicated by a v2 value of < 1.0 All other models had a v2 of > 1.0,...

Ngày tải lên: 30/03/2014, 15:20

... However, problems that occur during Pol II dependent expression of RNA virus proteins can be overcome by changing the codons of viral genes to those most frequently used by the genes of the host ... Figure of Analysis RSV-F mRNA processing Analysis of RSV-F mRNA processing A) Map of exon-intron structure and poly(A) signals of the precursor mRNA encoded by pIFwt Arrows indicate location of primers ... levels of the respective viral proteins Insertion of intron A of the CMV-IE gene resulted in mRNA levels comparable to those obtained by codon optimised expression plasmids in case of VSV-G or by...

Ngày tải lên: 18/06/2014, 18:20

... However, problems that occur during Pol II dependent expression of RNA virus proteins can be overcome by changing the codons of viral genes to those most frequently used by the genes of the host ... Figure of Analysis RSV-F mRNA processing Analysis of RSV-F mRNA processing A) Map of exon-intron structure and poly(A) signals of the precursor mRNA encoded by pIFwt Arrows indicate location of primers ... levels of the respective viral proteins Insertion of intron A of the CMV-IE gene resulted in mRNA levels comparable to those obtained by codon optimised expression plasmids in case of VSV-G or by...

Ngày tải lên: 20/06/2014, 01:20

... tissues Out of the 2,067 total differentially expressed genes identified, 127 genes were found to be differentially expressed in both tissues, 115 of which were consistently differentially expressed, ... controlled by a single incompletely dominant gene, denoted Se [7,8] A unique feature of semigamy in cotton is that expression of the trait in terms of haploid production is controlled by the genotype of ... found to be differentially expressed in the semigametic genotype 57-4 compared to its non-semigametic isogenic line Pima S-1 (Additional file and 2) Of the 284 differentially Page of expressed...

Ngày tải lên: 11/08/2014, 11:21

... genes in order of significance by applying each procedure to a large number of subsets of arrays of a given size These genes were then compared with the 'master' list of differentially expressed genes ... identification of the smallest groups of most differentially expressed genes, which would probably be deemed most important by an investigator as they are easiest to validate Overall, the scale of ... number of patients are randomly selected from each group and a variety of methods were used to determine a list of differentially expressed genes We are mainly interested in very small sizes of one...

Ngày tải lên: 14/08/2014, 14:21

... being hybridized provided that the majority of genes are not differentially expressed [5] What is not clear is how large the proportion of differentially expressed genes can be before lowess becomes ... percent of genes differentially expressed is varied If the normalization procedure is robust to the outliers then M = (a) In this scenario print-tip lowess is robust to approximately 20% of differentially ... differentially expressed genes when the default number of iterations is equal to (b) For 10 robustifying iterations the normalization is reliable for approximately 25% of genes differentially expressed...

Ngày tải lên: 14/08/2014, 17:22

... measures For each cut-off q,(0 ≤ q ≤ 1), we obtain the number of differentially expressed genes for each of the two lists as O1+(q) and O+1(q) and the number O11(q) of differentially expressed genes ... list of genes is divided into four groups: genes differentially expressed in both experiments, genes differentially expressed in only one of the two experiments, and genes differentially expressed ... influence of the prior specification on the posterior distribution of θ(q) The posterior distribution of R(q)|O, n can be easily derived from that of θ(q) using for example a sample of values of θ(q),...

Ngày tải lên: 14/08/2014, 20:22

... the potential of intact-cell MALDI- TOF MS (ICMS) Initial applications of ICMS on filamentous fungi have demonstrated characteristic sets of ions for strain identi cation in mycelia [4–8] and spores ... Neuhof et al Fig Intact-cell MALDI- TOF spectra of mycelia (A, C, E) and sporulating mycelia (B, D, F) of three strains of H jecorina grown on malt agar The masses displayed have an error of about ... (AVGA) and disulfide bond formation, has a mass of 7743 Da T longibrachiatum The main mass peak of T longibrachiatum of m ⁄ z 7242 was assigned to an HFB3-type of hydrophobin identified by searching...

Ngày tải lên: 19/02/2014, 02:20

... modifications mainly induced by OH• is the formation of carbonyl residues on amino acid side chains of proteins [43,64] In order to explore the ability of httEx1-polyQ to oxidize cellular proteins, ... inclusions formed by httEx1-103Q No significant modulation of inclusion size by NAC was detected These findings confirm the results of several other studies performed in cellular models of polyQ disease ... impaired clearance of oxidized proteins [35] Heat shock or stress proteins (Hsps) are expressed in neurons of polyQ diseased brains and have recently been identified as potent inhibitors of polyQ toxicity...

Ngày tải lên: 19/02/2014, 06:20

... supply of triterpene saponins by chemical synthesis is difficult both in terms of quantity and cost, biological production has been considered to be an alternative method to obtain them in sufficient ... initial cyclization of 2,3-oxidosqualene, a common precursor of all the sterol and triterpene biosyntheses, into various cyclic triterpenes, followed by oxidative modi cation of these carbon skeletons ... identified as the products of heterologously expressed triterpene synthases [10,14,17,20,24] Although the presence of some triterpenes (lupeol, b-amyrin, etc.) was confirmed in the whole plant by...

Ngày tải lên: 19/02/2014, 07:20

... 633 Figure mRNA expression of three isoforms of porcine ACL by RT-PCR Error bars indicate the SD (n = 4) of relative ACL mRNA expression levels to GAPDH, determined by RT quantitative PCR The ... Differential expression of porcine ACL gene 631 Figure Identi cation of EST39 an up-regulated gene in the backfat of F1 hybrids as compared with their parents (A) Silver staining of mRNA differential ... activity of ACL is regulated by diet regimen and insulin [6] It is generally believed that changes in ACL activity in terms of the de novo lipogenesis state are due to alterations in the rate of its...

Ngày tải lên: 14/08/2014, 13:21

... clones had changes of two amino acids, namely Glu315fiMet (mutant E315M), Glu315fiGln (mutant E315Q), and Asp392fiAsn (mutant D392N) Using the same expression and puri cation systems, the mutated and ... times at 20 °C by the addition of acetic acid to a final concentration of 10% and the glycosylation products were analyzed by using the calibrated HPLC -MS The chitooligomers formed by the wild-type ... to control and calibrate HPLC -MS data Preparation of chitinase A Native chitinase A secreted by V carchariae culture was purified by chitin-affinity binding and gel filtration chromatography following...

Ngày tải lên: 20/02/2014, 01:20

... study has highlighted the importance of Arg273 in binding of the C-terminus of peptide substrates by ACE2 The complete loss of activity observed as a result of replacing this single residue could ... Fig Schematic view of the active site of ACE2 and tACE Binding interactions of the inhibitor (A) MLN-4760 at the active site of ACE2 and (B) lisinopril at the active site of tACE Hydrogen bonds ... nitrogen) of both residues are within hydrogen-bonding distance of the carbonyl oxygen of the amide group of residue P1¢ (tACE) (Fig 1B) or equivalent terminal carboxylate oxygen (ACE2) (Fig 1A) of...

Ngày tải lên: 20/02/2014, 01:20

... radioactive peptide peaks purified by HPLC after phosphorylation by CKIa were analysed by ion-trap mass MS We identified the presence of two doubly charged peptides (M + H)2+, of masses 783.7 and 824.0 ... Ser14 The figure represents the release of 32P at each cycle of Edman degradation on covalently coupled peptide after phosphorylation of syntaxin-1A (1–265) by CKII and puri cation by HPLC Result ... phosphorylates in vitro syntaxin A on Thr21 Release of 32 P at each cycle of automatic Edman degradation after phosphorylation of syntaxin-1A by CKIa and puri cation by HPLC Peptides (fraction 16 from Fig...

Ngày tải lên: 22/02/2014, 04:20