fi cation of contamination and cross hybridization problems
Tài liệu Báo cáo khoa học: Direct identification of hydrophobins and their processing in Trichoderma using intact-cell MALDI-TOF MS docx
... potential of intact-cell MALDI-TOF MS (ICMS) Initial applications of ICMS on filamentous fungi have demonstrated characteristic sets of ions for strain identi cation in mycelia [4–8] and spores ... with verified cleavage sites observed for HFB1 and HFB2 and known sites for signal peptidases and Kex2-type peptidases (Table 1) Veri cation of these assessments by tryptic digestion and sequencing ... Neuhof et al Fig Intact-cell MALDI-TOF spectra of mycelia (A, C, E) and sporulating mycelia (B, D, F) of three strains of H jecorina grown on malt agar The masses displayed have an error of about...
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Báo cáo khoa học: Identification of malic and soluble oxaloacetate decarboxylase enzymes in Enterococcus faecalis potx
... for extract) and after (Pur for purified) Ni2+-affinity column puri cation MM, molecular mass standard markers (B) Zymograms for malic activity 10 lg of each purified recombinant CitM and MaeE proteins ... citM Cloning, heterologous expression and characterization of CitM and MaeE from E faecalis Initially, citM and maeE genes were amplified using specific primers and DNA extracted from E faecalis JH2-2 ... Interestingly, the presence of NAD+ and NADH caused inhibition of CitM OAD activity but not of MaeE (Table 1) Moreover, we assayed the effect of ATP and ADP on the activity of these enzymes since...
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Báo cáo khoa học: Phylogenetic comparison and classification of laccase and related multicopper oxidase protein sequences pot
... in bacteria and their classi cation and function are still controversial The first report of a bacterial laccase was from the Gram-negative soil bacterium Azospirillum lipoferum [33] and the enzyme ... value of 3.5, an optimal pH for ABTS of 2.7 and for syringaldazine of 5–5.5 The properties for Lcc2 are quite different with a pI value of 6.2–6.8, optimal pH for ABTS of and for syringaldazine of ... speciation and diversi cation of the Homobasidiomycete fungi Evidence for different functions of the various laccases is provided by expression studies and biochemical characterizations of different...
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Báo cáo khoa học: Structural identification of ladderane and other membrane lipids of planctomycetes capable of anaerobic ammonium oxidation (anammox) pptx
... chromatograms of (A) the TLF of a 99.5% pure suspension of Candidatus ‘B anammoxidans’ strain Dokhaven, and (B) the TLF after acid hydrolysis of the residue of the cell material of Candidatus ‘K ... chromatograms of the TLFs of (A) a 99.5% pure suspension of Candidatus ‘B anammoxidans’ strain Delft after base hydrolysis of the cell material, and (B) a mixed bacterial culture in which 81% of the ... Dokhaven of Candidatus ‘B anammoxidans’ Glycerol diethers and mixed glycerol ether/esters The last part of the chromatogram of the TLF shows a complex mixture (Fig 1A) of compounds which were identified...
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TimeML: Robust Specification of Event and Temporal Expressions in Text doc
... attribute structure of TIMEX, [(eid signalID relType)] Moreover, as the speci cation of the values for the eventRelType and timeRelType attributes of EVENT and the relType attribute of TIMEX indicates, ... respectively And, of course, since relType is defined on TIMEX, not EVENT, it must repeat the speci cation of permissible values All these considerations suggest that these triplets of attributes ... has been expanded to also mark polarity indicators such as not, no, none, etc., as well as indicators of temporal quanti cation such as twice, three times, and so forth The speci cation for SIGNAL...
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Báo cáo khoa học: Identification of CD4 and transferrin receptor antibodies by CXCR4 antibody-guided Pathfinder selection pot
... enrichment of output phage Identi cation of Jurkat cells that bind PhAbs Clones from each round of Pathfinder and Step-back selection in the presence of guide molecules (mAb 12G5 for Pathfinder, biotinlyated ... of the eluted biotinylated phage were used to infect an exponentially growing culture of E coli TG1 for ampli cation and preparation of PhAbs for further rounds of selection Phage titration and ... characterization of selected scFvs showed that none of them were CXCR4 specific, although antibodies against CD4 and transferrin receptor (TfR, CD71) were identified Some of the limitations of these selection...
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Energy and Environmental Problems behind China’s High Economic Growth – A Comprehensive Study of Medium- and Long-term Problems, Measures and International Cooperation pptx
... desertification poses a serious problem as well 1.75 million km2, accounting for 18% of China’s total land area, have already turned into deserts, and the areas of desertification are still expanding ... namely, creation of a Department of Energy as the government office responsible for all energy affairs, innovation of environmental control and administrative systems, and reforms of the political ... expanding at the fast rate of 3,436 km2 a year In addition, arable lands are decreasing by 300 to 600 thousand hectares yearly and deterioration of soils is advancing Natural grasslands are also disappearing...
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báo cáo hóa học: " Type D personality in the general population: a systematic review of health status, mechanisms of disease, and work-related problems" docx
... support, and they were Mols and Denollet Health and Quality of Life Outcomes 2010, 8:9 http://www.hqlo.com/content/8/1/9 Page of 10 Table List of criteria for assessing the methodological quality of ... correct method of calculating Type D Mols and Denollet Health and Quality of Life Outcomes 2010, 8:9 http://www.hqlo.com/content/8/1/9 Page of 10 Table Outcomes of studies: Medical and occupational ... article as: Mols and Denollet: Type D personality in the general population: a systematic review of health status, mechanisms of disease, and work-related problems Health and Quality of Life Outcomes...
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Báo cáo hóa học: "Research Article Adaptive Kernel Canonical Correlation Analysis Algorithms for Nonparametric Identification of Wiener and Hammerstein Systems" potx
... avoid zero solutions of (1): the first coefficient of the linear filter H(z) was fixed to 1, thus fixing the scaling factor and also the norm of all filter coefficients With the estimated filter represented ... and, therefore, also obtains good performance 5.2 Online identi cation In a second set of simulations, we compare the identi cation performance of the three adaptive kernel CCA-based identi cation ... adaptive version of kernel CCA which can be used for online identi cation of Wiener and Hammerstein systems 4.1 Formulation of KCCA as coupled RLS problems The special structure of the GEV problem...
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Báo cáo hóa học: " Research Article Classification of Single and Multiple Disturbances in Electric Signals" ppt
... processing timp tnot tdec tdam Classifier Classifier Classifier Classifier r Classifier Classifier Classifier Figure 4: Novel paradigm for the classi cation of single and multiple disturbances associated ... classi cation techniques, has been investigated and researchers in this field have achieved a great level of development [3–28] As a result, the current classi cation techniques are capable of classifying ... incidence of several sets of classes of disturbances can be identified easily In fact, each of the vectors f, h, i, timp , tnot , tdec , and tdam are related to disjointed classes of disturbances and...
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Báo cáo y học: "Associations of physical and mental health problems with chronic cough in a representative population cohort" potx
... 6-item instrument that includes items relating to frequency and intensity of dyspnea and wheeze and frequency of coughing and volume of sputum production Chronic cough was defined as cough reported ... but as 97% of the households in the region have telephones and the demographic characteristics were representative of the population of profile of Adelaide overall [37,38], the extent of any bias ... conceiving the analysis and for drafting of the manuscript SA undertook the analysis and contributed to revision of the manuscript DW, AT and RR contributed to the conception and conduct of 18 19 Morice...
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Báo cáo sinh học: " Identification of gametes and treatment of linear dependencies in the gametic QTL-relationship matrix and its inverse" pptx
... G∗ , and finally, the computation of its inverse It is independent of the mode of gamete identi cation and can be used with Qi definition (2a) as well as with Qi definition (2b) First part of the ... (2s-1)th and (2s)th column equal to the first and second column of Qi and the (2d-2)th and (2d)th column equal to the third and fourth column of Qi , all other elements of Ai are zero, where s and ... defined Non-base animals have Qi s with first and the second row sums equal to one as well as the sum of the elements of the sire block (first two columns of Qi ) and the sum of the elements of...
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A cross culture study on using gestures of vietnamese and american people
... Aims of the study This study is targeted at helping English learners to get enough information and full understanding of the classification of body language and how to use gestures in Vietnam and ... language and gestures The definition, features and classification of culture, body language, nonverbal communication and gestures are involved Besides, the similarities and differences of body ... Nonverbal communication, like verbal communication is also a part of culture and the carrier of a certain culture The functions of nonverbal communication in the whole communication can’t be overlooked...
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A CASE STUDY ON COMMON PROBLEMS IN LEARNING BUSINESS ENGLISH VOCABUALRY IN THE BOOK “BUSINESS BASICS” FACED BY THE 1ST YEAR STUDENTS AT VIETNAM UNIVERSITY OF COMMERCE, AND SOME SUGGESTED SOLUTIONS
... of them use flied instead of flew; or in terms of nouns, they often confuse between the singular and plural form of the nouns like businessman & businessmen; woman & women, both in spelling and ... high levels of proficiency Word lists and flash cards can be used for the initial exposure to a word, and can be arranged to create logical groupings of the target words (Gairns and Redman, op ... opportunities to learn and develop their knowledge in categories of grammar, vocabulary, communication skills, and pronunciation through practice activities of listening, speaking, reading, and writing...
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Tài liệu Báo cáo khoa học: Identification of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt
... G-quadruplex DNA and RNA Fig S2 CD spectra of DNAs and RNAs Fig S3 Binding affinity of EWS and RBD to Htelo 998 Fig S4 In vitro arginine methylation of RGG3 by PRMT3 Fig S5 Identi cation of significant residues ... 4) and 32P-labeled ETS-1 (lanes and 4) or ssDNA L (lanes and 2) (B) EMSA was performed with EWS (lanes 2, and 6) and 32P-labeled Htelo (lanes and 4), dsHtelo (lanes and 6) or ssDNA S (lanes and ... RGG3 (lanes and 4) and 32P-labeled Htelo (lanes and 2) or rHtelo (lanes and 4) (B) EMSA was performed with RGG3 (lanes and 4) and 32P-labeled rHtelo (lanes and 2) or mut rHtelo (lanes and 4) (C,...
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Tài liệu Báo cáo khoa học: Post-translational modification of the deubiquitinating enzyme otubain 1 modulates active RhoA levels and susceptibility to Yersinia invasion pptx
... The mean and standard deviations of the absolute numbers of observed colonies are indicated overexpression of OTUB1 and invasion with Y pseudotuberculosis Conversely, OTUB1 knockdown significantly ... and OTUB1–probe adduct were visualized by immunoblotting using HA antibodies and quantified for two experiments (black and grey bars) The intensities of the corresponding bands were measured and ... particular the S18E and Y26E variants, both of which exert increased affinity to YpkA The effect of these modifications on OTUB1 binding to YpkA did not fully account for loss of increased susceptibility...
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Tài liệu Báo cáo khoa học: The cellulosomes from Clostridium cellulolyticum Identification of new components and synergies between complexes ppt
... FEBS 3079 Diversity of cellulosomes and their synergies I Fendri et al Table Identi cation of specific components detected in fractions F1, F5 and F6 using MS analysis All identifications were based ... mass tolerance of 150 p.p.m., one missing cleavage allowed, a fixed modi cation of cysteines by iodoacetamide (carbamidomethyl), a variable modi cation of methionines (oxidation) and N-term glutamine ... 3083 Diversity of cellulosomes and their synergies I Fendri et al oxidation, cysteine carbamidomethylation and no fixed modi cation, and 1.5 and 1.0 Da as the maximum precursor and fragment tolerance...
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Tài liệu Báo cáo khoa học: A facile method for expression and purification of the Alzheimer’s disease-associated amyloid b-peptide pdf
... E22Q and D23N), and the availability of a rapid and simple expression and puri cation protocol will facilitate large-scale investigations of the molecular determinants of aggregation and fibrillation ... (Fig 2E) Puri cation of Ab(M1–40) and Ab(M1–42) The present work describes a rapid and inexpensive puri cation scheme to produce high-purity Ab(M1–40) and Ab(M1–42) in 24 h The puri cation scheme, ... (2% gel), and the cleaved product was purified using the GFX PCR and gel band puri cation kit The purified cut gene was ligated into PetSac vector (a modified from of Pet3a with NdeI and SacI cloning...
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Tài liệu Báo cáo khoa học: DNA modification with cisplatin affects sequence-specific DNA binding of p53 and p73 proteins in a target site-dependent manner pptx
... PGM1 and PGM4 targets) Effects of DNA cis-platination on sequencespecific DNA binding of p73 proteins We tested the influence of DNA modi cation with cisplatin on the binding of two p73 isoforms, ... unmodified targets in the absence of the competitor fragments (first samples of each set) were taken as For other details, see Figs and of the particular p53DBS The frequency of DNA modi cation ... IACs, 10% of 1,3-GNG IACs and interstrand crosslinks, and another 2–3% of monofunctional adducts It has been found that cisplatin cytotoxicity is related mainly to the IACs that induce significant...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25