κb in vascular smooth muscle cells

... c-Jun may be involved in Ang II-induced AGT mRNA expression (Fig 1C) DNA-binding activity of STAT5B and c-Jun increases in Ang II-induced AGT expression To find out whether the increase in STAT5B ... assays (EMSAs) using radiolabeled oligonucleotides containing either a STAT5B-binding site or an AP-1-binding site in the rat AGT gene promoter As shown in Fig 2A,B, DNA–protein complexes were ... MA, Lanting L, Todorov I & Natarajan R (2005) Angiotensin II 1728 24 25 26 27 28 29 30 31 32 33 34 35 enhances interleukin-18 mediated in ammatory gene expression in vascular smooth muscle cells: ...

Ngày tải lên: 30/03/2014, 02:20

... sonicated in a bath-type sonicator for 5min The amount of protein of the solubilized cells was determined with a bicinchoninic acid protein assay kit (PIERCE, Rockford, IL, USA) using bovine serum ... with 10% fetal bovine serum in a CO2incubator at 37 °C with CO2 in humidified air Then, the medium containing serum was removed, and the cells were washed with phosphate buffered saline Next, mL of ... induce apoptosis in various cell lines [8–15,29], the inhibitory effect of TS on the cell growth at higher concentrations might prevent the increase in the enhancing effect of TS on LPS/IFN-induced...

Ngày tải lên: 22/02/2014, 04:20

... Parkinson’s disease Homocysteine has also been shown to be toxic to neurons in culture by increasing the vulnerability of these cells to excitotoxic and oxidative injury [50] In smooth muscle cells, ... [31,34–36] As binding of Ab to the basement membrane is an early step in Ab-induced VSMC toxicity, we have examined the effect of substrate-bound Ab on the growth of vascular smooth muscle cells in culture ... Amyloid b-protein induces its own production in cultured degenerating cerebrovascular smooth muscle cells J Neurochem 65, 931–934 Davis-Salinas, J & Van Nostrand, W.E (1995) Amyloid b-protein aggregation...

Ngày tải lên: 22/02/2014, 07:20

... leptomeningeal smooth muscle cells Amyloid 1994, 1:1-7 Davis J, Cribbs DH, Cotman CW, Van Nostrand WE: Pathogenic amyloid β-protein induces apoptosis in cultured human cerebrovascular smooth muscle cells ... we determined the effects of dexamethasone on Aβ-induced pathologic responses in human cerebrovascular smooth muscle (HCSM) cells, a well-established in vitro model of CAA Our results indicate ... proteins, the culture medium was collected and the cells rinsed with phosphate-buffer saline The cells were then solubilized in a buffer consisting of 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 containing...

Ngày tải lên: 19/06/2014, 22:20

... has inspired much interest Both types of kinases add phosphate groups to residues near the actinbinding sites at the C-terminal region of CaD, thereby decreasing its effectiveness of actin binding, ... F-actin was stained with rhodaminephalloidin and incubated for h Finally, cell-loaded coverslips were rinsed and mounted on glass slides in Mowiol (Sigma) Images were obtained using a laser scanning ... untrasnfected cells and the EGFP-expressing control cells The green fluorescence in these cells overlapped closely with the red phalloidin staining (Fig 1-c), indicating that the expressed CaD mutant binds...

Ngày tải lên: 10/08/2014, 05:21

... deleterious loss of vascular structural integrity is associated with GM-CSF overstimulation in macrophages and smooth muscle cells Journal of Cardiothoracic Surgery 2010 5:66 Competing interests The ... accumulation of infiltrating cells particularly at the intima-media border (arrows indicate areas with profound vascularization) MMP1 (panel D) and MMP9 (panel E) were strongly expressed at the intima-media ... matrixmetalloproteinase activity at the intima-media border (rich in activated 27F10-positive inflammatory cells) and in association with clusters of CD68-positive macrophages in deeper medial...

Ngày tải lên: 10/08/2014, 09:22

... isolated cells were phenotypically characterized using muscle- specific antibodies against smooth muscle actin and myosin Cells were maintained in DMEM supplemented with 10% (v/v) fetal bovine serum ... and stained for F-actin with rhodamine-conjugated phalloidin The effects of toxin B and latrunculin B on the actin filaments was examined by preincubating the cells with either 10 ngÆmL)1 toxin B ... levels in response to anisomycin, suggesting that an intact actin cytoskeleton is also necessary for anisomycin signaling Surprisingly, treatment of the cells with latrunculin B alone induced an increase...

Ngày tải lên: 19/02/2014, 16:20

... SMC, by inhibiting signalling molecules [45–47] Caveolin-3 is the main isoform in muscle cells, including SMC [30] Caveolin-1 and caveolin-3 seem to act as scaffolding proteins that negatively regulate ... (1988) Interleukin 1: a mitogen for human vascular smooth muscle cells that induces the release of growth-inhibitory prostanoids J Clin Invest 81, 487– 498 Anderson, K.M., Roshak, A., Winkler, ... Jak/STAT signaling and is involved in plateletderived growth factor BB-induced growth in vascular smooth muscle cells J Biol Chem 278, 9986–9992 Nunez, E.A (1997) Fatty acids involved in signal cross-talk...

Ngày tải lên: 19/02/2014, 16:20

... signaling molecules are involved in these protein kinase pathways Conclusions In summary, this study presents evidence that protein kinases play an important role in regulating SOCs in smooth muscles ... Figure Diagram highlighting protein kinase modulation of SOCs in rat PASMCs Note that protein kinase G (PKG) and protein kinase A (PKA) inhibit SOC currents whereas protein kinase C (PKC) appears ... signaling pathway characteristically attenuates SOC activity in smooth muscle [19-21] This is exemplified by the inhibition of SOCs not only in A7R5 cells [22] but in native smooth muscle cells...

Ngày tải lên: 10/08/2014, 05:21

... submerged in freezing embedding medium (2.5% polyvinyl alcohol) and placed in liquid nitrogen, sliced by a freezing microtome, fixed with acetone for min, washed with PBS for 10 min, and blocked in ... the calcium sensing receptor in regulating intra-cellular calcium handling in human epidermal keratinocytes J Invest Dermatol 2007, 127:1074-1083 Zhang WH, Xu CQ: Calcium sensing receptor and ... described [12] Briefly, cells were washed three times with ice-cold phosphate-buffered saline (PBS) and then incubated in cool protein lysate containing the protease inhibitor phenylmethyl sulfonyl...

Ngày tải lên: 10/08/2014, 05:21

... (FRET)/emission ratioing using a classic laser point scanning microscope, this is associated with a number of challenging technical issues (see introduction) In these studies we therefore used a spinning disk ... CD)-VENUS in human primary airway smooth muscle (hASM) cells By transfecting this probe into hASM cells, we were able to image and quantify pharmacological and temporal information regarding cyclic ... system in which to investigate ligand-directed signaling at endogenous b adrenoceptors Utilising HEK293-GloSensor cells and human bronchial smooth muscle cells both endogenously expressing b2-adrenoceptors...

Ngày tải lên: 12/08/2014, 13:22

... simvastatin in human bronchial smooth muscle cells[ 29] In addition to the mevalonate pathway, Insulin-like growth factor I/insulin dependent pathway has been implicated in the inhibition of human vascular ... simvastatin to sildenafil also reversed hypoxia-induced pulmonary hypertension and remodelling[16] In keeping with findings in animal experiments, recent clinical study using simvastatin in PAH ... successful in reversing pulmonary vascular remodelling in animals [33,34], including the use of simvastatin in monocrotaline- and hypoxia-induced PH [17,18] However, the apoptotic response to statins...

Ngày tải lên: 12/08/2014, 14:20

... by ER depletion is the dominated component of CCE [16] Ca2+ influx via SOC appears to be a determinant in maintaining a sustained increase in [Ca2+]i and regulation of vascular tone and arterial ... factor, ng/mL heparin, mg/mL insulin and 0.2 mg/mL bovine serum albumin (BSA) Cells were maintained at 37°C in a humidified normoxia (21% O2, 5% CO2, 74% N2) and passaged after reaching 80-90% confluence ... activity in skeletal muscle cells and smooth muscle cells [15] http://respiratory-research.com/content/10/1/123 Two main types of calcium channels in the human PASMC membrane mediate Ca2+ influx:...

Ngày tải lên: 12/08/2014, 14:20

... corresponding the consensus (NF -κB- consensus) or putative cd38 (NF-CD38) NF -κB binding sequences Note NF -κB binding (indicated by horizontal arrow) in samples obtained from TNF-α-treated cells Binding ... nicotinamide adenine dinucleotide (NAD) [1] CD38 is expressed in different cells including airway smooth muscle (ASM) cells, where its expression is confined to the plasma membrane [4] In ASM cells, ... Figure TNF-α-induced activation and specific binding of NF -κB to the consensus and cd38 putative binding sites in HASM cells TNF-α-induced activation and specific binding of NF -κB to the consensus...

Ngày tải lên: 12/08/2014, 15:21

... effective at inhibiting vascular smooth muscle cell proliferation [15,22] The mechanisms underlying remodelling of pulmonary arteries in PAH are multifactorial and include abnormalities in signalling ... of PDE4 in modulating vascular structure is unclear, studies to date indicating that when used alone PDE4 inhibitors are capable of suppressing the migration of isolated smooth muscle cells [20,21], ... placed in Dulbecco's modified Eagle medium (DMEM) containing 20% (v/v) foetal bovine serum (FBS) and 1% (v/v) antibiotic/antimycotic at 37°C, 5% CO2 Cells were maintained in DMEM containing 5–10%...

Ngày tải lên: 12/08/2014, 16:20

... propose a paracrine interaction between smooth muscle cells, neurones, inflammatory cells and structural cells in the vicinity, in which neurotrophins derived from bronchial smooth muscle may promote ... [6] Since mast cells have been shown to be in close contact with smooth muscle cells in the asthmatic bronchus [39], smooth muscle cell-derived NGF has the potential to influence airway mast cells ... mediators in BDNF induction Our finding that secretion of BDNF, but not NGF, was inhibited by the unselective COX inhibitor indomethacin and the COX-2 inhibitor NS-398 imply the prostaglandins as...

Ngày tải lên: 12/08/2014, 16:20

... epithelial cells, increase the likelihood of RV infecting the underlying smooth muscle during a naturally acquired RV infection in asthmatic subjects Human airway smooth muscle (HASM) cells are ... from infected HASM cells UVi rhinovirus induces cytokine release from infected HASM cells Cytokine release in response to rhinovirus infection, and exposure to UVi rhinovirus (UVi), of HASM cells ... Rhinovirus infection of HASM cells induces necrotic cell death Rhinovirus infection of HASM cells induces necrotic cell death Photomicrographs of HASM cells 24 hours post: A) Infection with rhinovirus...

Ngày tải lên: 12/08/2014, 16:20

... transports in rat airway smooth muscle cells Analysis of chloride transports in rat airway smooth muscle cells A Immunofluorescence study of α -smooth muscle actin in tracheal smooth muscle cells from ... Immunofluorescence study of CFTR in TSMC and D in the absence of primary antibody α -smooth muscle actin is stained in red, CFTR in green and nucleus (TOPRO-3 staining) in blue Scale bars are 10 µm ... times in Tris-buffered saline (TBS) and after fixation, non specific binding sites were blocked with TBS containing 0.5% BSA and 0.05% saponin for h Cells were incubated with an anti-CFTR C-terminal...

Ngày tải lên: 12/08/2014, 16:20

... localisation of vinculin spots displayed in (A) binding partners, including a-actin [39] Changes in the relative content of vinculin and a-actin have been reported in the human aortic intima of patients ... element-binding protein in sera of giant cell arteritis patients Protein extract is from vascular smooth muscle cells (VSMCs) (A) IgG reactivity to far upstream element-binding protein (FUBP2) in five ... Protein network generated by merging the two pathways involved in target antigens Protein extracts are from human umbilical vein endothelial cells and vascular smooth muscle cells Solid lines indicate...

Ngày tải lên: 12/08/2014, 17:22

... Outward currents or no response were evoked in the remaining cells, which were not studied further In most cells the inward currents activated in an oscillating manner (Figure 1) The first current ... slow agonist administration elicits much slower and smaller currents in vascular smooth muscle cells [36] Although this would be disadvantageous if studying P2X receptors, by minimizing the P2X response ... receptor in SPA smooth muscle [6] is most likely to underlie the remaining suramin- and PPADS-sensitive component Interestingly, contractions of the rat LPA were abolished by both suramin and PPADS,...

Ngày tải lên: 12/08/2014, 18:20