κb iκb protein subunits present in human vascular smooth muscle

... sonicator for 5min The amount of protein of the solubilized cells was determined with a bicinchoninic acid protein assay kit (PIERCE, Rockford, IL, USA) using bovine serum albumin as a standard ... clear as the effects observed in the system with LPS/IFN Effects of protein kinase inhibitors on the enhancement by TS of LPS/IFN-induced NO production Protein kinases such as PKA and PKC, and ... extracellular signal-regulated kinase and c-Jun NH2-terminal kinase but not p38 mitogen-activated protein kinases is required for RRR-a-tocopheryl succinate-induced apoptosis of human breast cancer cells...

Ngày tải lên: 22/02/2014, 04:20

... co-precipitate with a class of 143-3 proteins and, moreover, competitively inhibit the binding of phosphorylated cofilin to 14-3-3 proteins Using SPR-based evaluation of protein interactions, we found that ... hill-and-valley appearance, and showed positive staining for the smooth muscle- specific protein a-actin and calponin In the present study, we used cells in passages 3-7 Unless otherwise specified, serum-deprived ... the highest binding affinity for the g isoform of 14-3-3 proteins (Figure 1B) Hence, we focused on pHSP20-14-3-3 g protein interactions in human ASM as a potential molecular target against excessive...

Ngày tải lên: 12/08/2014, 13:22

... corresponding the consensus (NF -κB- consensus) or putative cd38 (NF-CD38) NF -κB binding sequences Note NF -κB binding (indicated by horizontal arrow) in samples obtained from TNF-α-treated cells Binding ... analyzed using GeneQuest for the potential transcription factor binding sites using tfd.dat file Analysis revealed six AP-1 binding sites, one NF -κB binding site and four GRE binding sites within the ... binding of GR to cd38 putative GRE binding sites Specific Specific binding of GR to cd38 putative GRE binding sites Electrophoretic mobility gel shift assays demonstrating binding of nuclear proteins...

Ngày tải lên: 12/08/2014, 15:21

... NGF protein; (C) BDNF mRNA and (D) BDNF protein The IL-1βdependent effects on neurotrophin mRNA expression and secreted protein are expressed as change in neurotrophin mRNA (%) and protein release ... propose a paracrine interaction between smooth muscle cells, neurones, inflammatory cells and structural cells in the vicinity, in which neurotrophins derived from bronchial smooth muscle may promote ... further investigation In both our, and in the study by Freund and co-workers, NGF protein induction was detected with a significant delay The dramatic differences in mRNA and protein induction...

Ngày tải lên: 12/08/2014, 16:20

... signaling pathways including PI3kinase, Rac and other Rho family G protein members in a variety of cell types, including vascular smooth muscle cells Rac has an essential role in cell migration and ... CD23 (C) nor changes in phenotype (A) These findings were confirmed by three independent observers Table 2: IL-4/GM-CSF Combination Increases Protein Content in huASMC Cytokine (nM) BSA (vehicle) ... increase in cell volume and protein content, cytoskeletal depolymerization, cell spreading and membrane ruffling Because ASMC require a doubling time of 48 hours, the increase in protein content...

Ngày tải lên: 13/08/2014, 13:22

... reducing and denaturing conditions Protein separation was performed applying SDS-PAGE and using systems containing 6% acrylamide in stacking gels and 12% in the separation gel Tris-glycine was ... taken (inset) The arrowhead in the inset indicates COMP staining (f) At gw 12, COMP staining is found in the outer regions of the diaphysis and is mainly pericellular (inset) Bars = 70 µm in (f), ... promoter contains a typical consensus site for binding to LRF/factor binding inducer of short transcripts protein- 1 (FBI-1) [15] If FBI-1 [43] acts as human counterpart of murine LRF, human COMP...

Ngày tải lên: 09/08/2014, 07:20

... glucosamine was ineffective in preventing phosphorylation of this kinase JNK and p38 are involved in cellular stress; they are phosphorylated in response to proinflammatory cytokines, resulting in ... N-acetylation; the resulting N-acetylglucosamine can modify several proteins by coupling itself to the serine or threonine residues of those proteins These include kinases, phosphatases, transcription ... NH2-terminal kinase, p38mitogen-activated protein kinase and activating protein- I J Pharmacol Exp Ther 2003, 305:981-988 40 Ho LJ, Lin LC, Hung LF, Wang SJ, Lee CH, Chang DM, Lai JH, Tai TY: Retinoic...

Ngày tải lên: 09/08/2014, 10:21

... Vpx proteins has already been reported [40] C) Only Vpx proteins from the SIVSM/HIV-2 lineage increase WT HIV-1 LVs infection in a pre-incubation assay Non-infectious SIVMAC VLPs containing the ... proteins were produced and used as described in A in a pre-incubation assay to test their effect on WT HIV-1 LVs infectivity (used at a constant MOI of 3) Incorporation of Flag-Vpx proteins into ... R, Weiler S, Lang SM: Vpx proteins of SIVmac239 and HIV-2ROD interact with the cytoskeletal protein alpha-actinin J Gen Virol 2004, 85:3291-3303 Mahalingam S, Van Tine B, Santiago ML, Gao F,...

Ngày tải lên: 13/08/2014, 09:20

... assays (EMSAs) using radiolabeled oligonucleotides containing either a STAT5B-binding site or an AP-1-binding site in the rat AGT gene promoter As shown in Fig 2A,B, DNA protein complexes were ... c-Jun may be involved in Ang II-induced AGT mRNA expression (Fig 1C) DNA-binding activity of STAT5B and c-Jun increases in Ang II-induced AGT expression To find out whether the increase in STAT5B ... oligonucleotide probes containing the AP-1-binding site (A) and the STAT5B-binding site (B) in the AGT gene promoter Protein DNA complexes were separated by nondenaturing PAGE and then visualized...

Ngày tải lên: 30/03/2014, 02:20

... signaling molecules are involved in these protein kinase pathways Conclusions In summary, this study presents evidence that protein kinases play an important role in regulating SOCs in smooth muscles ... Figure Diagram highlighting protein kinase modulation of SOCs in rat PASMCs Note that protein kinase G (PKG) and protein kinase A (PKA) inhibit SOC currents whereas protein kinase C (PKC) appears ... iPLA2: Ca2+-independent phospholipase A2; Ins(1,4,5)P3: inositol 1,4,5triphosphate; PASMC: pulmonary artery smooth muscle cell; PKA: protein kinase A; PKC: protein kinase C; PKG: protein kinase G;...

Ngày tải lên: 10/08/2014, 05:21

... regulation involves at least different transcription factor binding sites [33]: cAMP response element binding protein (CREB), CCAAT/enhancer-binding protein (C/EBP) binding site, activator protein (AP)-1, ... cells, increase the likelihood of RV infecting the underlying smooth muscle during a naturally acquired RV infection in asthmatic subjects Human airway smooth muscle (HASM) cells are actively involved ... Maskeri N, Hodinka R, Grunstein MM: Rhinovirus-mediated changes in airway smooth muscle responsiveness: induced autocrine role of interleukin-1beta Am J Physiol 1999, 277:L13-L21 Grunstein MM, Hakonarson...

Ngày tải lên: 12/08/2014, 16:20

... monolayer of cells lining the inner wall of blood vessel and works as a barrier separating the blood flow and vascular muscle cells As continuously exposed to the flowing blood, vascular endothelial ... substances in one signaling pathway will increase the difficulty in experiment and sometimes may bring unnecessary measurement error 1.3 Thesis Objective and Outline In this thesis, an engineering approach ... shear-stressinduced calcium response in VECs– because: • Human intervention of cell behavior is still in its infant stage and it’s wiser not to make the problem too complicated • Information encoding...

Ngày tải lên: 09/09/2015, 18:48

... massive matrixmetalloproteinase activity at the intima-media border (rich in activated 27F10-positive inflammatory cells) and in association with clusters of CD68-positive macrophages in deeper medial ... data on vascular matrix integrity and expression of the major matrix molecules indicate that although both collagen and elastin production was stimulated in SMC, the even greater increase in MMP ... gelatinases (MMP2 and MMP9) (CSx) compared with controls (ctrl) exceeding elastolysis and collagenolysis and thus a dramatic loss of structural integrity Interestingly, in areas of exceeding matrix...

Ngày tải lên: 10/08/2014, 09:22

... cAMP-elevating agent β2-agonist fenoterol in bradykinin-induced IL-8 release from hTERT-airway smooth muscle cells As illustrated in Fig 1A, bradykinin induced an increase in the release of IL-8 from the ... Rap2 are present in hTERT-airway smooth muscle cells in both membrane and cytosolic compartments Interestingly, activation of PKA and Epac induced GTPloading of Rap1 in hTERT-airway smooth muscle; ... Augmentation of bradykinin-induced IL-8 release in hTERT-airway smooth muscle cells by Epac and PKA Augmentation of bradykinin-induced IL-8 release in hTERT-airway smooth muscle cells by Epac...

Ngày tải lên: 12/08/2014, 14:20

... [3H]ICI198,615 binding and LTD4-induced [3H]thymidine incorporation Figure RT-PCR, [3H]ICI198,615 binding and LTD4-induced [3H]thymidine incorporation (A) Final RT-PCR products obtained using inner primers ... analysis) The protein content was determined by Lowry quantization assay Non specific binding ranged between 40 and 50% of the total binding [3H] Thymidine incorporation assay [3H]Thymidine incorporation ... cysteinyl leukotrienes in airway inflammation, smooth muscle function, and remodeling J Allergy Clin Immunol 2003, 111(1B):S18-S36 Billington CK, Penn RB: Signaling and regulation of G proteincoupled...

Ngày tải lên: 12/08/2014, 16:20

... transports in rat airway smooth muscle cells Analysis of chloride transports in rat airway smooth muscle cells A Immunofluorescence study of α -smooth muscle actin in tracheal smooth muscle cells ... Immunofluorescence study of CFTR in TSMC and D in the absence of primary antibody α -smooth muscle actin is stained in red, CFTR in green and nucleus (TOPRO-3 staining) in blue Scale bars are 10 µm ... http://respiratory-research.com/content/7/1/113 arginine [29] This observation is consistent with other findings showing decreased exhaled NO in patients with CF [30-32] and a reduction in NOS expression in CF murine and human airway...

Ngày tải lên: 12/08/2014, 16:20

... preparations, including (1) laminin from EHS tumours that contains laminin type (laminin 111) isoform, (2) affinity purified β1 chain-containing laminin from human placenta that includes laminin-1 (laminin ... values for laminin chain mRNA expression Gene product Laminin chain mRNA (absolute ΔCT values) Day Laminin α1 chain Laminin α2 chain Laminin β1 chain Laminin β2 chain Laminin γ1 chain Day 22.2 ... 111), (laminin 211), (laminin 311), (laminin 411), 10 (laminin 511) isoforms, and (3) affinity purified α2 chain-containing laminin from human placenta that consists of laminin-2 (laminin 211)...

Ngày tải lên: 12/08/2014, 16:20

... element-binding protein in sera of giant cell arteritis patients Protein extract is from vascular smooth muscle cells (VSMCs) (A) IgG reactivity to far upstream element-binding protein (FUBP2) in five ... localisation of vinculin spots displayed in (A) binding partners, including a-actin [39] Changes in the relative content of vinculin and a-actin have been reported in the human aortic intima of patients ... VSMCs and HUVECs Vinculin is a cytoskeleton protein involved in extracellular matrix adhesion and intercellular junctions by binding to actin filaments This protein has several interaction sites...

Ngày tải lên: 12/08/2014, 17:22

... immunostaining Expressioncell MMP-12 protein in bronchial smooth muscle Expression of MMP-12 protein in bronchial smooth muscle tissue and cell cultures by immunostaining Sections from human bronchial ... ECM proteins, including elastin, type IV collagen, fibronectin, laminin and gelatin [8,9], and is involved in turnover of the matrix, cell migration, tissue repairing and remodeling In addition, ... appearance and by positive immunostaining of smooth muscle (SM) α-actin, SM myosin heavy chain, calponin and SM-22 The cells were maintained in T175 culture flasks at 37°C in a humidified atmosphere of...

Ngày tải lên: 12/08/2014, 18:20

... apolipoprotein E bicinchoninic acid basic fibroblast growth factor calmodulin calcium/calmodulin-dependent protein kinase kinase C-terminal HSC70-interacting protein E3 ubiquitin ligase C-type lectin ... of proteins The domains include an N-terminal kinase domain, a calmodulin (CaM) binding domain, a series of eight ankyrin repeats, two P-loops, a cytoskeletal binding domain, and a death domain ... kappa B alpha IkB kinase interleukin 18 interleukin beta interleukin c-Jun kinase low-density lipoproteins LDL receptor mitogen-activated protein kinase mini-chromosome maintenance complex component...

Ngày tải lên: 24/08/2014, 11:25