γ h2ax formation as an essential step in biological processes
Báo cáo khoa học: Functional analysis of cell-free-produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formation pptx
Ngày tải lên :
16/03/2014, 10:20
... fitted by using : Langmuir models The determined kd was used for calculation of ka, and the binding constants KD were determined from kd ⁄ ka We determined the binding constant KD for binding of ETBcHx ... area spanning I138-I197, and thus covering TMS2 and TMS3, as the ET-1-binding site [44] In addition, other regions, such as TMS5, have been proposed to be involved in ligand binding as evaluated ... findings, and they further define TMS1 as a central determinant for ET-1 binding On the basis of chimeric ETB derivatives and binding of antagonists, Wada et al proposed a 60 amino acid area spanning...
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Tài liệu Báo cáo khoa học: An intermediate step in the evolution of ATPases – a hybrid F0–V0 rotor in a bacterial Na+ F1F0 ATP synthase pdf
Ngày tải lên :
18/02/2014, 17:20
... two covalently linked hairpins in the membrane [10] Importantly, the ion-binding site is not conserved in hairpin one If one assumes the same number of transmembrane helices in V0 and F0, the rotor ... increasing the laser intensity, led to the detachment of detergent and water molecules 2002 Moreover, additional energy was transferred into the system, and the c rings (partly) dissociated into ... c2 ⁄ As observed before for the c rings of entire Na+ F1F0 ATP synthases [18,19] as well as isolated c rings [20], the A woodii c ring was highly stable and did not dissociate by boiling in 20...
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Báo cáo khoa học: An intermediate step in the evolution of ATPases ) the F1F0-ATPase from Acetobacterium woodii contains F-type and V-type rotor subunits and is capable of ATP synthesis Michael Fritz and Volker Muller ¨ docx
Ngày tải lên :
23/03/2014, 09:20
... blotted against c1 antibodies Lane 4: ATP synthase was incubated for 10 at 80 °C, and blotted against c1 antibodies Lane 5: the sample was incubated for 10 at 80 °C and hybridized with antibody specific ... Lane 6: ATP synthase was incubated for at 120 °C and hybridized with antibodies against subunit c2 ⁄ (which also detect subunit c1) Lane 7: ATP synthase was denatured by boiling for 15 min, and ... subunit, giving rise to a 16 kDa protein with two hairpins [20] Most important, the ion-binding site is not conserved in hairpin If one assumes the same number of hairpins in V0 and F0, the...
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Molecular Imaging: An Essential Tool in Preclinical Research, Diagnostic Imaging, and Therapy potx
Ngày tải lên :
29/06/2014, 11:20
... publishers cannot guarantee the accuracy of any information about dosage and application contained in this book In every individual case the user must check such information by consulting the relevant ... 26 In a general sense, molecular imaging is a term used to define in vivo imaging of molecules and stands in contrast to anatomical or physiological imaging In that sense, any type of imaging ... the anti-VEGF activity (by decreasing plasma clearance of the aptamer) in in vitro inhibition assays of endothelial cells proliferation, and reduced vascular permeability and angiogenesis in vivo...
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Holcim has strengthened its balance sheet and earning power and positioned itself as an attractive group in the international capital markets
Ngày tải lên :
30/07/2014, 17:22
... 30 Cash flow from (used in) financing activities (C) 1,120 (984) In( De)crease in cash and cash equivalents (A+B+C) 1,340 (99) Cash and cash equivalents as at January 2,456 2,698 In( De)crease in ... Financial instruments Information about accounting for derivative financial instruments and hedging activities is included in the section “Financial Risk Management” Accounting Policies 107 Financial ... 12 Financial income (expenses) Other ordinary (expenses) income net Depreciation and amortization of non-operating assets Total The increase in financial income is mainly due to an impair- In...
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Báo cáo y học: "Hand bone loss as an outcome measure in established rheumatoid arthritis: 2-year observational study comparing cortical and total bone loss" pot
Ngày tải lên :
09/08/2014, 10:20
... nondominant hand [11] have all been used To avoid bias regarding dominant and nondominant hands and to achieve better precision, we used the mean of both hands Only patients who had complete measurements ... the change in the DXA hand and in the DXA total hip and spine were 0.35 (P < 0.001) and 0.18 (P = 0.01), whereas correlations between the change in the DXR hand and DXA total hip and spine were ... changes and increased inflammation in the small joints of the hand in the first years of the disease has been suggested partly to explain this finding [25] As DXA-BMD measures both trabecular and cortical...
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Báo cáo y học: " Spontaneous coronary artery dissection presenting as an ischaemic stroke in a middle-aged man with anti-cardiolipin antibodies: a case report" ppsx
Ngày tải lên :
11/08/2014, 12:20
... causing a stroke, which was his presenting complaint The patient was anticoagulated with warfarin and secondary prevention instituted with angiotensin-converting enzyme (ACE) inhibitor, statin and ... knowledge, in a middle-aged man in association with anti-cardiolipin antibodies Treatment of SCAD, although lacking in consensus, depends on the clinical situation If the patient is asymptomatic and ... conservative management is advised, as in the case of our patient Successful revascularization in acutely unwell patients, in the form of percutaneous coronary intervention [12] and coronary artery bypass...
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Báo cáo y học: "Citrullinated vimentin as an important antigen in immune complexes from synovial fluid of rheumatoid arthritis patients with antibodies against citrullinated proteins" pot
Ngày tải lên :
12/08/2014, 14:22
... citrullinated Fibβ and Fibγ in RA synovium has been reported by Matsuo et al [25] Based on these findings, immunodetection was performed with anti-Fibβ, anti-Fibγ, anti-fibronectin and anti-vimentin ... rabbit anti-human Fibβ, rabbit anti-human Fibγ and rabbit anti-human fibronectin Anti-rabbit HRP labelled antibody was used as a secondary antibody ECL detection was carried out by means of Supersignal ... performed with anti-fibrinogenβ (a), anti-fibrinogenγ (b), anti-fibronectin (c), anti-vimentin (d) and AMC staining (e) Note that different mass and pI scales were used for clarity Box × indicates...
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Báo cáo y học: " Bench-to-bedside review: The gut as an endocrine organ in the critically ill" pdf
Ngày tải lên :
13/08/2014, 20:22
... of gastric emptying and fasting cholecystokinin and peptide YY concentrations Relationship between the rate of gastric emptying (measured using an isotope breath test and calculated as the gastric ... in animals [30], and this may also be the case in humans (Dr G Kostuic, personal communication) Pharmacological doses of ghrelin also increase fasting blood glucose and suppress plasma insulin ... glucose-lowering reflects slower gastric emptying, as well as increased insulin and decreased glucagon secretion [66-68] Pharmacological doses of GLP-1 reduce both fasting and postprandial glycaemia...
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Mitsugumin 53 (MG53) as an e3 ligase in skeletal muscle
Ngày tải lên :
20/05/2016, 15:30
... 30 generate an activated integrin state at the cell surface.The integrin-binding proteins paxillin and talin recruit focal adhesion kinase (FAK) and vinculin to focal contacts -Actinin is a cytoskeletal ... possessing the HECT (homologous to the E6-AP carboxyl terminus) domain and those containing the RING (really interesting new gene)-finger domain (41, 43) 27 The RING-finger domain is a zinc finger ... Tyrosine Kinase RING Really Interesting New Gene si-RNA small interfering RNA IX SOCS Suppressor of Cytokine Signaling SPRY SPla and RYanodine receptor TRIM Tripartite Motif-containing Ub Ubiquitin...
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báo cáo khoa học: " Extensin network formation in Vitis vinifera callus cells is an essential and causal event in rapid and H2O2-induced reduction in primary cell wall hydration" ppsx
Ngày tải lên :
11/08/2014, 11:21
... resulted in substantial swelling, saline extraction after extensin network formation resulted in significantly less swelling This smaller increase in hydration after extensin network formation was ... Abdel-Massih RM, Baydoun EA-H, Brett CT: Protein and ph dependent binding of nascent pectin and glucoronoarabinoxylan to xyloglucan in pea Planta 2000, 211:423-429 48 Smallwood M, Bevan A, Donovan ... co-ordinate action of matrix modifying enzymes including expansin [10], xyloglucan endotransglycosylase/hydrolase (XHT) [11], a variety of glycosyl hydrolases and possibly some class III peroxidases...
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Báo cáo y học: " Formation of translational risk score based on correlation coefficients as an alternative to Cox regression models for predicting outcome in patients with NSCLC" doc
Ngày tải lên :
13/08/2014, 16:20
... in more than 85% of cases [1] It is the leading cause of mortality in terms of malignant disorders, and its incidence is increasing [2] The underlying pathology is complex and numerous proteins ... using significant correlations of survival time, with T, N and M being aggressive factors and CD68 and GAS6 being protective factors By including information relating to molecular markers and ... each, resulting in an integrated score for an individual risk (ISIR) The resulting ISIR score should predict the outcome, reflecting the individual balance between significant aggressive and protective...
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USING BRAND AS AN EFFECTIVE WEAPON TO COMPETE IN THE MARKET: A CASE STUDY OF NHAT LINH COMPANY
Ngày tải lên :
13/04/2013, 10:29
... Class Stretching the Stretching the Brand Vertically Brand Vertically in existing in existing Product Class Product Class Stretching Stretching Down Down Brand Brand Extensions Extensions in in ... play an increasingly important role Recently, the way in which branded products or services have been distinguished from one another has increasingly concerned non-tangible factors rather than tangible ... different Product Class Class Stretching Stretching Up Up Ad Hoc Ad Hoc Brand Brand Extensions Extensions Co-Branding Co-Branding Creating aa Creating Range Brand Range Brand Figure 2.3 : A Variety...
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THE INFORMAL SECTOR IN SOLID WASTE MANAGEMENT IN DEVELOPING COUNTRIES SUSTAINABILITY EFFECTS OF FORMALISATION CONSIDERING HO CHI MINH CITY – VIETNAM AS AN EXAMPLE
Ngày tải lên :
24/08/2013, 18:48
... Ho Chi Minh City (HCMC) as an example Florian Scheibe Matr Nr 9840238 THE INFORMAL SECTOR IN SOLID WASTE MANAGEMENT IN DEVELOPING COUNTRIES The motivation for recycling in developing and industrialised ... the value of the waste, and in the second place by their ease of extraction, handling and transport Paper, metals and plastics, sorted out as early as possible in the waste chain, therefore attract ... 2003) In many cases policies strive for the elimination of scavenging rather than trying to use their workforce In fact, the informal recycling sector is an already working subsystem in waste management...
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Tài liệu Báo cáo khoa học: NirF is a periplasmic protein that binds d1 heme as part of its essential role in d1 heme biogenesis pdf
Ngày tải lên :
15/02/2014, 01:20
... deletion was generated in nirF in a wild-type GB17 P pantotrophus strain This was performed in a two -step process First, the 5¢ and 3¢ flanking regions of nirF were cloned and the kanR cassette inserted ... kanamycin-resistance cassette, but lost the pJQ200ks backbone, were selected as kanamycin-resistant gentamicin-sensitive strains Correct integration of the cassette was confirmed by PCR screening ... and strategy employed can be found in supporting information (Doc S1, S2 and S3) Cloning of P pantotrophus nirF and nirF variants The nirF ORF was amplified from P pantotrophus genomic DNA using...
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Tài liệu Báo cáo khoa học: Control analysis as a tool to understand the formation of the las operon in Lactococcus lactis doc
Ngày tải lên :
19/02/2014, 17:20
... polymerase (New England Biolabs, Frankfurt am Main, Germany) was applied for analytical purposes and PCR products intended for cloning were generated using ElongaseR enzyme mix (Invi˚ trogen, Tastrup, ... However, organization of the las operon may also play an important role in keeping internal metabolite pools constant, by coregulating enzymes early and late in glycolysis when changes in the flux ... level and above Fig Glycolysis and the las operon in Lactococcus lactis.The las operon in L lactis consists of the three genes pfk, pyk and ldh, coding for phosphofructokinase (PFK), pyruvate kinase...
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Tài liệu Báo cáo khoa học: Nop53p, an essential nucleolar protein that interacts with Nop17p and Nip7p, is required for pre-rRNA processing in Saccharomyces cerevisiae pdf
Ngày tải lên :
20/02/2014, 01:20
... SalI) was inserted into the pET-28a vector digested with BamHI and XhoI restriction enzymes Yeast transformation and maintenance Yeast strains used in this work are listed in Table Yeast strains ... ORF and was identified in the yeast nuclear pore complex [29] and as a component of the pre60S complex [6,7] In this study, Nop53p was isolated in a two-hybrid screen as a protein interacting ... of L40, bearing plasmid pBTM-NOP53, which encodes a hybrid protein containing the lexA DNA binding domain and the full-length NOP53 ORF Transformation of YDG146 was performed with plasmid pGAD-NOP17...
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Báo cáo " Imperialism of communicative language teaching and possible resistance against it from teachers in Vietnam as an English foreign languages context " doc
Ngày tải lên :
05/03/2014, 12:20
... all participants in the classroom, and between these participants and the various activities and texts The second role is to act as an independent participant within the learning-teaching group… ... situations, meaning teaching with focus on language functions rather than the grammar, using more simulations, role plays, and games Another opinion is that I understand CLT as the language teaching method ... needs, and interests Since CLT was introduced, the teacher’s roles have been taken into consideration Breen and Candlin (cited in Richards and Rodgers (1986) [13]) claim: “The teacher has two main...
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Báo cáo khoa học: Neuroserpin Portland (Ser52Arg) is trapped as an inactive intermediate that rapidly forms polymers Implications for the epilepsy seen in the dementia FENIB ppt
Ngày tải lên :
07/03/2014, 16:20
... to that obtained for other serpins, including a1-antitrypsin and a1-antichymotrypsin [19,20] In comparison, the spectrum of Ser52Arg neuroserpin shows an increase in both b-sheet and a-helical ... neuroserpin (ÆÆÆÆ) and Ser52Arg neuroserpin polymers (- - -) and mutant neuroserpin The proteins were added to increasing concentrations of urea, and the change in fluorescence profile was followed ... neuroserpin: a neuronal serpin involved in a conformational disease FEBS Lett 25171, 1–5 16 Im, H., Woo, M.-S., Hwang, K.Y & M.-H (2002) Interactions causing the kinetic trap in serpin protein folding...
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