``in situ´´ sword of damocles

... performed Fixation and Cryosectioning of Biofilm Samples Biofilm samples were taken at regular time intervals during the periods of the biofilm development The biofilm samples were fixed with freshly ... steady-state microprofiles of O2, NH4+, NO2-, and NO3- in the autotrophic nitrifying biofilm (B) The distribution and magnitude of the estimated specific rates of net consumption and production of NH4+, ... rates of NH4+, NO2-, and NO3- The biofilm surface was at a depth of zero The values are means of triplicate measurements Microelectrode Measurements Steady-state concentration profiles of O2,...

Ngày tải lên: 05/09/2013, 08:40

... Nature of the Ends of the Fragments 3.3.1 Processing of Cells and Extraction of DNA Each flask should contain a minimum of 107 cells Remove the medium from each flask and set aside Add mL of trypsin/ ... cleavage of each strand of the DNA duplex is offset generating fragments with a 3' recess 1.1 The Nature of the DNA Fragments Endonucleases cleave DNA by attacking the phosphodiester bonds of the ... new microfuge tube Precipitate the DNA from this supernatant as in step Resuspend the precipitated DNA in 50 µL of TE buffer in a microfuge tube (see Note 19) 3.3.2 Labeling of 3'-OH Ends of DNA...

Ngày tải lên: 11/04/2014, 09:47

... heating and expansion of the air adjacent to the heated surface The remainder of the photographs are for experiments above the phase explosion threshold fluence of 2.0 J/cm2 Results of ablation at an ... (2002) 118–123 Fig Transient microphotographs of ablation at average fluence of 3.1 J/cm2 Fig Transient microphotographs of ablation at average fluence of 5.3 J/cm2 D.A Willis, X Xu / Applied Surface ... that is mounted on a micrometer stage Moving the position of the prism changes the path length of the probe laser and the arrival time of the probe beam (with respect to the pump beam) at the...

Ngày tải lên: 06/05/2014, 08:55

... stages of growth with a rate of -10°C/sec, and then transferred under UHV to the attached STM chamber Imaging was performed at tunneling currents of around 0.1 nA and applied sample bias of 2–4 ... have been found to be one of the major low-energy surfaces of silicon [21, 22], meaning that the sidewall facettation leads to a lowering of the total surface energy of the system After 20 nm ... depicted below the ridges and filling of the grooves (see STM image of Fig 3), which decreases the amount of (001) surface area on the stripes Toward the end of buffer growth at 520°C, however,...

Ngày tải lên: 21/06/2014, 08:20

... This chelation effect of CS–Fe complex is the base of mineralization of magnetite in chitosan hydrogel, as demonstrated in this article Also, XPS can provide the Fe content of the CS–Fe complex, ... Degradation of magnetite/chitosan composite by H2O2 caused slight oxidation of mineralized magnetite nanoparticles, which induced a slight increase of d by 0.06 approximately However, because of the ... coated by a thin layer of chitosan Moreover, the TEM morphology of mineralized magnetite nanoparticles proved the existence of chitosan layer In order to obtain the amount of chitosan layer on...

Ngày tải lên: 21/06/2014, 20:20

... profile of bFGF refers to the relatively released concentration of (C*/C0 ) as a function of t Here, C* represents the concentration of released bFGF in PBS at predetermined intervals of time ... viability (% of control) as a function of the concentration of MSNs during the period from day to day is shown in Fig The cell viability (% of control) are obtained by normalizing the value of the ... internalization of pure MSNs (50 lg/mL) was Cell viability (% of control) 180% Fig Confocal microscope image of the uptake of MSNs in cell (FITC from cell stain solution are absorbed on the surface of MSN...

Ngày tải lên: 22/06/2014, 00:20

... formation of Au–Pt NP assembly over a larger area, without much long range ordering An average height of 7.4 ± 1.3 nm is obtained for the Au–Pt NPs from the analysis of a number of AFM images of different ... analysis software Surface coverage of different nanoparticles was estimated by analyzing the mean grain area and number of grains present in a typical lm lm (total area = lm2) AFM image of the respective ... methodology as discussed above Figure shows the comparison of the UV–Visible response of pure Au NPs to that of Au–Pt NPs with different mole ratio of Au and Pt It is evident that Au NPs show a characteristic...

Ngày tải lên: 22/06/2014, 00:20

... temperature of weight loss of the P-MWNTs occurred at 600 °C while that of the F-MWNTs occurred at 460 °C The early weight loss of the F-MWNTs could be attributed to the stripping off of aromatic ... homogeneous dispersion of CNTs, the efficient and more or less destructive chemical modification of CNTs would be the best option [23] The aligned CNTs have been synthesized by the deposition of CNTs onto ... nanofibers with acid-treated MWNTs, and studied the effect of collector speed on the morphologies of the nanofibers and the crystal structures of the nylon-6 In the present study, we employed the aromatic...

Ngày tải lên: 22/06/2014, 01:20

... maximum of demand function crosses the C;regulation line We can observe that, if WUE of maize is higher than the WUE of lilac or potato, the junction occurs within the same range of values of g ... Bierhuizen J.F & Slatyer R.O (1965) Effect of atmospheric concentration of water vapor and C0 in determining transpiration photosynthesis relationship of cotton leaves Agric Meteorol 2, 259-270 ... ,4! values between versus 0.20 g, Up to and 0.23 , s m’ mol the g dependence of A is c c almost linear and the slope of this line represents C Beyond these values, i - C a A does not increase...

Ngày tải lên: 09/08/2014, 04:20

... diameter of airEffects ofvessels in (U4;absence of L-NG-nitro-L-arginine10-5 Figure way and2 U46619 or on M) and luminal slices methyl ester (L-NAME)10-7 internal acetylcholine (Ach; Effects of U46619 ... Figure slices of U46619 and M) and in whole internal cut-5 M), diameter of airway(10-7-7vessels acetylcholine (Ach; luminal charybdotoxin (Ch, 10 of (Ap+Ch), on × 10-7) 10 Effects Effects of U46619 ... M) in isolated pulmonary artery Characterisation of the dilator effects of acetylcholine (ACh; Figure Characterisation of the dilator effects of acetylcholine (ACh; 10-5 M) in isolated pulmonary...

Ngày tải lên: 12/08/2014, 16:20

... analyzed Of interest, we detected clusters of Gag CM9 cells that may be indicative of recent clonal expansion of these cells To our knowledge, we are the first to show accumulation of Gag CM9 ... 0.31% of the CD8+ T cells of any of the single cell suspensions described above bound to the Qdot 655 multimer loaded with the negative control peptide FLP, verifying that nonspecific binding of ... lymph nodes Both of these anatomical sites are a part of the GALT, which is considered to comprise most of the secondary lymphoid organ system and harbors the largest number of recently activated...

Ngày tải lên: 12/08/2014, 23:23

... Region A representative spectra of the far-infrared region, 400-700 cm-1, are shown in Figure G.1 Figure G.1 In-situ raw far-infrared spectra of the hydroformylation of 3,3-dimethyl-1-but-ene in ... 248 APPENDIX B Fluxionality Of Tetrarhodium Dodecacarbonyl Cluster 249 APPENDIX B Fluxionality Of Tetrarhodium Dodecacarbonyl Cluster 250 APPENDIX B Fluxionality Of Tetrarhodium Dodecacarbonyl ... 251 APPENDIX B Fluxionality Of Tetrarhodium Dodecacarbonyl Cluster 252 APPENDIX B Fluxionality Of Tetrarhodium Dodecacarbonyl Cluster 253 APPENDIX B Fluxionality Of Tetrarhodium Dodecacarbonyl...

Ngày tải lên: 14/09/2015, 12:54

... N ke×E Estimated sum of moles of element E nstd Number of moles of the internal standard species PCO Partial pressure of CO gas in bar sat Psolv Saturated vapor pressure of solvent R Universal ... Enthalpy of activation ΔS‡ Entropy of activation ΔrG Equilibrium thermodynamic free energy of reaction ΔrH Equilibrium thermodynamic enthalpy of reaction ΔrS Equilibrium thermodynamic entropy of reaction ... Initial rate of reaction of the rate of formation of (μ4-η2Figure 6.28 C2H5CCC2H5)Rh4(CO)8(μ -CO)2 during the CO-Variation batch experiments …………………………………………………………… 161 Figure 6.29 The orders of Rh4(CO)9(μ-CO)3,...

Ngày tải lên: 14/09/2015, 13:04

... curves of pH dependent dyes 25 2.4.3 Fusion of Microfluidics and Optics Very recently, there’s the trend of development of optofluidics, the fusion of microfluidics and optics [47] The idea of optofluidics ... applications of microfluidic systems 5 Figure 2.1 Various microfluidic systems from Syrris-dolomite 2.2 Microfluidics and Cell Culture Among aforementioned different applications, the use of microfluidics ... Microchannel Fabrication Proof of concept of the fluorescence quenching system is just one aim of the preliminary study; another task of this part is about the fabrication of the sensor chip Using...

Ngày tải lên: 03/10/2015, 11:36

... consumption of the acetaldehyde added and the production of equal amounts of 3-hydroxypropanoate, while the concentration of transient 3-ketopropanoate was too low to be detected Addition of mM acetaldehyde ... metabolism (A) Spectrum of the substrate (B) Spectrum after 36 h Small amounts of acetealdehyde and 3-ketopropanoate were identified by the addition of standard solutions Larger amounts of acetate and ... and does not affect growth rates by means of isotopic effects [16] Addition of defined amounts of D2O, however, is useful to determine the incorporation of protons from the solvent into the products...

Ngày tải lên: 08/03/2014, 08:20

... the decomposition of PANI As seen from the TGA curves, the rate of weight loss of PANI (curve a) is much fast, the residue of PANI is 44.06%; while the rate of weight loss of PANI/magnetite nanocomposites ... remarkable decrease of Ms values should be attributed to the formation of many magnetite particles absorbed on the surface of PANI nanorods As shown in Fig 4, the magnetic hysteresis loops of PANI/magnetite ... the ice-water bath Then, ml of X Ding et al / Journal of Colloid and Interface Science 320 (2008) 341–345 343 Fig Schematic illustration of the synthesis process of PANI/magnetite nanorod composites...

Ngày tải lên: 20/03/2014, 13:04

... specificity of Tgases and have expanded the range of application of the enzyme reaction [20] TGase 3, initially designated as an epidermal-type enzyme, is responsible for formation of the epidermis ... concerted reactions result in formation of the cornified envelope (CE), a specialized component consisting of covalent crosslinks of proteins beneath the plasma membrane of terminally differentiated keratinocytes ... localization of activated TGase 3, which was found to display a pattern distinct from that of TGase 1, was observed by the detection of in situ activities using this peptide Results Screening of candidate...

Ngày tải lên: 29/03/2014, 21:20

... Department of Oncology, University Hospital of North Norway, Tromso, Norway 2Institute of Clinical Medicine, University of Tromso, Tromso, Norway 3Department of Pathology, University Hospital of North ... al Journal of Translational Medicine 2011, 9:6 http://www.translational-medicine.com/content/9/1/6 Page of by the log rank test DSS was determined from the date of surgery to the time of lung cancer ... 4Institute of Medical Biology, University of Tromso, Tromso, Norway 5Department of Pathology, Nordland Central Hospital, Bodo, Norway Authors’ contributions TD participated in the design of the study,...

Ngày tải lên: 18/06/2014, 16:20

... imply the induction of directed formation of bone essential for spinal arthrodesis [10] Often heterotopic bone bridging transverse processes of the vertebrae was not capable of restricting intervertebral ... testing Of note, it is unclear what effect, if any, fixation has on the mechanical attributes of the tissue For mechanical testing spines were first cast in the center of a × × cm block of dental ... was directed parallel to the long dimension of the plastic pieces Palpation Integrity of the fusions was qualitatively confirmed after removal of soft tissues with bleach and manual palpation...

Ngày tải lên: 20/06/2014, 04:20

... imply the induction of directed formation of bone essential for spinal arthrodesis [10] Often heterotopic bone bridging transverse processes of the vertebrae was not capable of restricting intervertebral ... testing Of note, it is unclear what effect, if any, fixation has on the mechanical attributes of the tissue For mechanical testing spines were first cast in the center of a × × cm block of dental ... was directed parallel to the long dimension of the plastic pieces Palpation Integrity of the fusions was qualitatively confirmed after removal of soft tissues with bleach and manual palpation...

Ngày tải lên: 20/06/2014, 07:20