number size shape and properties of cells
Tài liệu Nature and Properties of Micro-organisms doc
... (VZV) VZV: Chickenpox VZV: Shingles Re-activation of latent infection Suppression of cell mediated immunity Bacteria Unicellular, DNA and RNA Binary No prokaryotes fission mitochondria ... Classified according to: Gram stain reaction size & shape cell wall (Gram stain) aerotolerance positive - purple negative - pink Shape coccus - round (chains? clusters?) bacillus ... reducing opsonisation and protecting from phagocytosis E.g Streptococcus pneumoniae in RTI vaccination: pneumococcal; meningococcal A and C GLYCOCALYX loose meshwork of fibrils extending...
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Tài liệu Báo cáo khoa học: Identi®cation and properties of type I-signal peptidases of Bacillus amyloliquefaciens doc
... Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipV Cloning of sipV Cloning of sipV Cloning of sipV ... Cloning of sipV Cloning of sipV Cloning of sipV Construction of pOpacVh Construction of pOpacVh Cloning of sipW Cloning of sipW Cloning of sipW Cloning of sipW Cloning of sipW Cloning of sipW ... Construction of pOpacWh Construction of pOpacWh Construction of pOpacSh Construction of pOpacSh Construction of pOpacTh Construction of pOpacTh Construction of pOpacBh Construction of pOpacBh Construction...
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Báo cáo khoa học: Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family pdf
... moiety of Arg51 and three atoms of FMN (N1, O2 and O3) In both FeR and M acetivorans avoredoxin, the corresponding residue is asparagine (Asn47 and Asn52, respectively) to which only the O2 atom of ... the Km and kcat values of E coli Fre for riboavin are reported as 2.5 lm and 52.4 s)1 with NADPH as the electron donor and 1.3 lm and 30.6 s)1 with NADH [13] For FMN, Km and kcat values of E coli ... of the logarithms of the concentration ratios for oxidized and reduced forms of the mediator versus that of recombinant avoredoxin Reduction titration of avoredoxin by NAD(P)H The reduction of...
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Báo cáo khóa học: Localization, purification and properties of a tetrathionate hydrolase from Acidithiobacillus caldus pot
... purification yield of the enzyme may be due to several reasons Copper and several other divalent ions stimulated the activity of the tetrathionate hydrolase and they and other cofactors may be present ... hydrolase of A caldus (Eur J Biochem 271) 279 The biochemical properties of tetrathionate hydrolase of A caldus were similar to those of other acidithiobacilli (Table 4) The specific activity of tetrathionate ... method of Lowry et al [27] using the BCA Protein Assay Kit (Pierce, Rockford, IL, USA) The molecular mass and subunit structure of the tetrathionate hydrolase were determined using MALDI-TOF MS and...
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Báo cáo khoa học: Origin and properties of cytoplasmic and mitochondrial isoforms of taurocyamine kinase pptx
... LK and AK, in agreement with the proposed relationship between the size of guanidine substrate and the number of amino acids deleted [9] In addition, the amino acid sequence of GS region of cytoplasmic ... other hand, mitochondrial TK showed activity for taurocyamine, lombricine (30% of that of taurocyamine) and glycocyamine (7% of that of taurocyamine) Recombinant Arenicola MiTK was incapable of phosphorylating ... Alignment of mitochondrial targeting sequences of vertebrate and invertebrate MiCKs and Arenicola MiTK Sequences correspond to ubiquitous (uMiCK) and sarcomeric (sMiCK) MiCKs from man and chicken and...
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Báo cáo khoa học: Purification and properties of a new S-adenosyl-Lmethionine:flavonoid 4¢-O-methyltransferase from carnation (Dianthus caryophyllus L.) pot
... the method of Baayen and Elgersma [13], with a 500-lL drop of Fod conidial suspension at a concentration of · 106 conidiaÆmL)1; 20 additional plants, inoculated with a 500-lL drop of double distilled ... NaPi buffer, pH 3, and acetonitrile (6 : 1, v/v); separation was performed isocratically, at a flow rate of mLÆmin)1, and the volume of injected samples was 10 lL The amounts of the residual initial ... by the addition of two drops of 10 M HCl Aliquots of each reaction mixture were analyzed through column chromatography [7] to separate and purify both the assayed substrates and the respective...
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Báo cáo Y học: Molecular cloning, bacterial expression and properties of Rab31 and Rab32 docx
... lg of platelet protein and 0.5 lg of GST and GST±Rab proteins; (B) an [a-32P]GTP overlay [17] of a nitrocellulose blot of a gel containing 40 lg of platelet protein, 0.5 lg of GST and 0.5 lg of ... form of Rab32 (see Discussion) Fig Puri®cation and properties of wild-type and mutant Rab31 and Rab32 expressed as GST±fusion proteins Samples of platelet particulate fraction protein and of puri®ed ... liver and kidney, and was also found in HEL and K562 cells (Fig 3) A 2.0-kb Rab32 mRNA was also detected in some preparations of RNA from HEL cells To demonstrate the presence of Rab31, Rab32 and...
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Mechanics and Properties of Composed Materials and Structures doc
... The effect of geometrical parameters and material properties of face sheets and core are studied on the face wrinkling of sandwich plates Comparison of the present results with those of plate theories ... anisotropic properties of strength and stiffness of masonry in horizontal and vertical direction, tensile and shear stiffness of cracked masonry, • Concrete yielding in compression, opening of cracks ... tension, mechanism of opening and closing of cracks in concrete under dynamic load, tensile and shear stiffness of cracked concrete, • Strain rate effect of the material properties of masonry, reinforced...
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Báo cáo Y học: Trehalose-phosphate synthase of Mycobacterium tuberculosis Cloning, expression and properties of the recombinant enzyme pdf
... the cloning and expression of the M tuberculosis tps gene in E coli, and the production of active TPS in these cells The properties of the recombinant enzyme have been determined, and are compared ... M) of NH4HCO3 An aliquot of each fraction was removed and assayed for its radioactive content The radioactive peak was pooled and concentrated to dryness a number of times in the presence of ... mammalian cells but it appears to play an important role as a structural component of the M tuberculosis cell wall, and might also function as a stabilizer and protector of membranes and proteins...
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preparation and properties of magnetic iron oxide nanotubes
... spectra of samples S2 (a) and S3 (b) can be exclusively indexed to ␣-Fe2 O3 , according to standard data (JCPDS 33-0664) In Fig 1(b) and (c), the reflection peaks of XRD patterns of S2 and S3, ... that sample S2 is Fe3 O4 and sample S3 is ␥-Fe2 O3 Fig presents the SEM and TEM images of samples S1, S2 and S3 Fig 3(a) and (b) show the morphologies of initial synthesized ␣-Fe2 O3 nanotubes ... in sample S3 (e and f) B Lv et al / Particuology (2008) 334–339 and outer and inner diameters of 70–120 nm and 45–80 nm, respectively Fig 3(c) and (d) show the morphologies of Fe3 O4 nanotubes...
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Báo cáo khoa học: Effect of mutations in the b5–b7 loop on the structure and properties of human small heat shock protein HSP22 (HspB8, H11) pptx
... The N- and C-terminal domains are indicated by N and C correspondingly All b-strands are numbered and the b5 and b7 strands are shown in red and blue, respectively G104 (equivalent to K137 of human ... the effect of a mutation in this part of the molecule on the structure and properties of human sHsp Oligomeric structure of HSP22 and its mutants All samples of recombinant HSP22 and its mutants ... loop and in the N-terminal part of the b7 strands destabilize the structure of HSP22 Limited trypsinolysis of the wild-type HSP22 and its K137E and K137,141E mutants Fig Far-UV CD spectra of the...
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Báo cáo khoa học: Trehalose synthase of Mycobacterium smegmatis Purification, cloning, expression, and properties of the enzyme ppt
... solution and all run at the same time in the same solvent Standards of trehalose and maltose are applied to the sides of the paper to determine the locations of these sugars, and those areas of the ... 0.5, and 10 mM initial concentrations of maltose (left side) or trehalose (right side) Samples were removed at times up to 22 h Properties of the TreS purified from M smegmatis Effect of time and ... formation and characterization of the products The conversion of trehalose to maltose was measured by determining the amount of reducing sugar resulting from the production of maltose The amount of...
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Báo cáo khoa học: Purification and properties of the glutathione S-transferases from the anoxia-tolerant turtle, Trachemys scripta elegans pdf
... studies characterized the properties of GST in hydroxylapatite Peaks and from control liver Isoelectric focusing Isoelectric focusing of GSTs from liver of aerobic and anoxic turtles is shown ... hydroperoxide as a substrate was not detected in Peak off hydroxylapatite Substrate and inhibitor profiles of Peaks and GST isozymes off of hydroxylapatite from aerobic control liver are summarized ... activities, and kinetic properties of the enzyme The current data document the presence of two isozymes of GST in turtle liver that are separable by column chro3608 W G Willmore and K B Storey...
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Báo cáo Y học: Purification and properties of the extracellular lipase, LipA, of Acinetobacter sp. RAG-1 docx
... toward medium and long chain esters of pNP Maximal reaction temperature is 55 °C and LipA shows strong activity in the presence of metals, inhibitors, and organic solvents Based on these properties, ... (1999) Cloning and sequence analysis of the lipase and lipase chaperone- encoding genes from Acinetobacter calcoaceticus RAG-1, and redefinition of a proteobacterial lipase family and an analogous ... appearance of a zone of clearing Effect of pH on lipase activity and stability Concentrated lipase preparations were diluted fourfold in NaH2PO4-NaOH buffer (50 mM) at various pH values and incubated...
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Báo cáo Y học: Puri®cation and properties of an alkaline proteinase of Fusarium culmorum doc
... buffer and 200-drop fractions were collected The absorbance values of these and of the chromatography fractions in subsequent steps were measured at 280 nm and their nonspeci®c proteinase and chymotrypsin/subtilisin-like ... 4.5, 5.0 and 5.5 (Na acetate), pH 6.0, 6.5 and 6.9 (Na citrate), pH 6.9, 7.5, 7.9, 8.3, 8.7 and 9.1 (Tris/HCl) and pH 9.2, 9.6, 10.0 and 10.4 (Caps) All buffers were 80 mM The activities of the ... in 30 mM buffers: Na acetate, pH 4.1 and 4.9; Na citrate, pH 5.9 and 6.4; or Tris/HCl, pH 7.7 and 8.5 The activity of each sample was measured after and 90 of incubation For measuring its thermal...
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processing and properties of nanocomposites, 2007, p.463
... discovery of fullerenes, and the structural perfection of lijima's nanotubes which implied a whole range of exciting properties CNTs have typical diameters in the range of ~1—50 nm and lengths of many ... This general concept of exploiting the inherent properties of nanoscaled materials is not limited to the mechanical properties of a material, since a wide range of physical properties also depend ... Processing and Properties of NAN0C0MP0S1TES Processing and Properties of AN0C0MP0S uresh G Advani University of Delaware, USA \fc world Scientific JEW JERSEY...
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structure and properties of atomic nanoclusters, 2005, p.427
... consists of the reduction of Fe2+ and Nd3+ salts using borohydride ions in aqueous solution The concentration and speed of addition of the reactants, the control of the pH, the temperature and the ... semiconductor superlattices of high quality Use of a beam of clusters, instead of atoms, would allow the production of more complex superstructures 10 Structure and Properties ofAtomic Ciusiers 2.2 ... experiments [3] of intensity peaks at Nel4 and Ne56(instead of the usual Ne13 and Ne55), at ArI4 and Ar148 (instead of ATl3 and Ar147) ,and at (CH4)14 (instead of (CH4)13); that is, peaks shifted by...
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Báo cáo hóa học: " Preparation and properties of copper-oil-based nanofluids" ppt
... r and ν are the density and kinematic viscosity of the nanofluid, respectively, at the same temperature Measurements of the thermal conductivities of Cu nanofluids were performed by means of ... shown in Figure 2b, the bands of C-H and OCH2 are also observed in the surface-modified Cu nanoparticles, while the absorption peaks of P = S and P-S shifts, and the bands of N-H mostly disappear ... additives in oils Viscosities and thermal conductivities of nanofluids The effects of both temperature and mass fraction of the nanoparticles on the viscosities of the nanofluids were investigated...
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Báo cáo hóa học: "Fabrication and Properties of Porphyrin Nano- and Microparticles with Novel Morphology" docx
... dipole of CoTPPS4- and that of H2TAPP4+ in the formation process of porphyrin nanoand micro-particles [33, 34] The Soret and Q bands of sample solution placed for 72 h are not shifted, and the ... is one of the proofs that the pyramids consist of CoTPPS4- and H4TAPP6+ Figure 1c, d shows the images of samples and 3, respectively In Fig 1c, the diameter of the spheres is in the range of 250–1,300 ... the shape and the size distribution of the porphyrin particles For one thing, it is the molar ratios of two kinds of porphyrin in the solution The structures of two porphyrins (Scheme 1) and...
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