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Studies on preservation of porcine zygotes for embryo production by interspecies somatic cell nuclear transfer

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Mammals are remarkable organisms that have different morphologies with important characteristics allowing scientists to create animal model systems. The establishment of these animal systems is especially important for further investigations because it is exceedingly difficult to establish an appropriate model for research in complex animal species, including humans. In recent years, the major challenge for the field of reproductive biotechnology has been exploring the molecular and cellular mechanisms that are involved in controlling the quality of oocytes. The mammalian oocyte is a specific structure consisting of cytoplasmic organelles that communicate among themselves and are spatially associated. The porcine species represents an excellent experimental model for primary and biomedical research because of their physiological and immunological resemblances with humans 1, 2. The porcine embryo production in vitro is especially important in reproductive biotechnology for cloning and transgenesis since these animals are used as organ donors for xenotransplantation, embryonic stem cell lines, and also as a model of human diseases

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