Mục lục

  • Introduction

  • Fundamentals & Theory

    • The cell

      • The cell structure

      • Membrane potential

      • Action potentials and signal propagation

      • Signal propagation in cardiac cells

      • TRPV channels as temperature sensors

      • Cell Models

        • HL-1 cells: A cardiomyocyte cell line

        • PC12 cells as a model for neurons

        • Devices and fabrication techniques

          • Microwire array

          • Microfluidic channels for cell culture

          • Fabrication technologies for microfluidic channels

          • Heat stimulation

            • Resistive heating

            • Heat transfer

            • Optical detection

              • Calcium imaging

              • Fluorescence lifetime imaging (FLIM)

              • Heat stimulation for modulating signal propagation in HL-1 cell networks

                • Preamble

                • Introduction

                • Materials and methods

                  • Fabrication of microwire array chips

                  • Culture of HL-1 cells on the microwire array chip

                  • Thermal stimulation and Ca2+ imaging

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