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THÔNG TIN TÀI LIỆU
Cấu trúc
List of Tables
List of Figures
1. Introduction
1.1. Context
1.2. Microalgae and classification
1.2.1. General information on microalgae
1.2.2. Presentation of algae with commercial interest
1.2.2.1. Chlorophyta (Green algae)
1.2.2.3. Bacillariophyta (Diatoms)
1.2.2.4. Eustigmatophyta
1.3. Algal cultivation system
1.3.1. Closed photobioreactors (PBRs)
1.3.1.1. Tubular photobioreactors
1.3.1.2. Flat plate PBRs
1.3.2. High rate algal pond (HRAP)
1.3.3. Comparison of PBRs and HRAPs
1.4. High value components of microalgae
1.4.1. Proteins
1.4.2. Lipids
1.4.3. Carotenoids
1.5. Application of microalgae
1.5.1. Animal feedstock
1.5.2. Microalgae in aquaculture
1.5.3. Microalgae used for production of human food
1.5.5. Biofuel production
1.6. Bottlenecks in large - scale production of microalgae
2. Aim
3. Materials and methods
3.1. Materials (see Annex)
3.2. Methods
3.2.1. Isolation and purification of microalgae
3.2.2. DNA isolation and phylogenetic analyses
3.2.2.1. DNA isolation
3.2.2.2. Polymerase chain reaction (PCR) assay
3.2.2.3. DNA agarose gel electrophoresis
3.2.2.4. Phylogenetic analyses
3.2.3. Microalgal growth and biomass analysis
3.2.3.1. Microalgal growth
3.2.3.2. Biomass analysis
3.2.3.2.1. Dry weight
3.2.3.2.2. Fatty Acid Methyl Ester Analysis (FAME) analysis and estimation of biodiesel fuel properties
Fatty Acid Methyl Ester analysis
Estimation of biodiesel fuel properties based on FAME profiles
3.2.3.2.3. Protein analysis
3.2.3.2.4. Pigment analysis
4. Results and discussion
4.1. Isolation, identification and preliminary characterization of four microalgal isolates
4.1.1. Isolation and identification
4.1.2. Microalgal growth
4.1.3. Biomass analysis
We then analyzed the biomass composition in order to detect if some strains contained interesting valuable compounds.
4.1.3.1. Pigment profiles and protein content
4.1.3.2. Fatty acid profiles
4.2. Identification and characterization of strain nl3
The results published are obtained when cells are grown at 200 µmol m-2 s-1. We present in this section the published results as well as the others, which complement these findings. In rubrics 4.2.1 and 4.2.2, the Group I intron and Phylogenetic analy...
4.2.1. Sequencing of the rDNA-ITS Region
4.2.2. Phylogenetic analysis of the nl3 sequence
4.2.3. Characterization
4.2.3.1. Microalgal growth in TAP medium
4.2.3.2. Microalgal growth in TAP medium at different salinities
4.2.3.3. Effect of growth phase and salinity condition on fatty acid profile
4.2.3.4. Effect of salinity condition on pigment profile
4.2.3.5. Effect of salinity condition and growth phase on biodiesel quality
4.3. Identification and characterization of strain NL6
4.3.1. Sequencing of the rDNA –ITS Region
4.3.2. Characterization
4.3.2.1. Microalgal growth
4.3.2.2. Effect of growth phase, salinity and light intensity condition on fatty acid profile
Data in Table 4.10 showed that the fatty acid profiles of two light intensities of 50 µmol m-2s-1 and 200 µmol µmol m-2s-1 were similar. The fatty acid chain lengths ranged from C14 to C20, in which C16 and C16:1 were predominant. The fatty acid profi...
4.3.2.3. Effect of growth phase, salinity and light intensity condition on biodiesel quality
4.3.2.4. Effect of growth phase on pigment profile and protein content
2.4.3. Biodiesel Fuel Properties Based on FAME Profiles
2.4.4. Protein Content
2.4.5. Pigment Analysis
2.5. Intron Analysis
3. Results
3.1. Sequencing of the rDNA-ITS Region
3.2. Phylogenetic Analysis of the nl3 Sequence
3.3. Microalgal Growth
3.4. Pigment Content in the Exponential and Stationary Phases
3.5. Fatty Acid Profile and Content in the Exponential and Stationary Phases
3.6. Effect of Salinity Conditions on Biodiesel Quality
4. Discussion
5. Conclusions
7. Scientific activities
7.1. Participation at the 9th International Conference on Algal Biomass, Biofuels and Bioproducts, 17 – 19 June, 2019, Boulder, CO, USA (Poster), https://orbi.uliege.be/handle/2268/237197
7.2. Participation at AlgaEurope Conference, 3-5 December 2019, Paris, France (Poster), http://hdl.handle.net/2268/242916
References
Kaplan, D. (2007). Water pollution and bioremediation by microalgae: absorption and adsorption of heavy metals by microalgae, In: Richmond A., (Ed) Handbook of Microalgal Culture, pp. 439 - 447, Blackwell Publishing, Wiley, New York. https://doi.org/...