The effect of betel quid chewing on the absorption of cigarette smoke containing acrolein

THAI NGUYEN UNIVERSITY UNIVERSITY OF AGRICULTURE AND FORESTRY NGUYEN THI MAI CHI TOPIC TITLE: THE EFFECT OF BETEL QUID CHEWING ON THE ABSORPTION OF CIGARETTE SMOKE CONTAINING ACROLEIN BARCHELOR THESIS Study Mode: Full-time Major: Environmental Science and Management Faculty: International Programs Office Batch: 2013 - 2017 Thai Nguyen, 20/11/2017 DOCUMENTATION PAGE WITH ABSTRACT Thai Nguyen University of Agriculture and Forestry Degree Program Bachelor of Environmental Science and Management Student name Nguyen Thi Mai Chi Student ID DTN1353070121 Thesis Title THE EFFECT OF BETEL QUID CHEWING ON THE ABSORPTION OF CIGARETTE SMOKE CONTAINING ACROLEIN Supervisors Prof Tsung - Yun Liu Dr Do Thi Ngoc Oanh Abstract: Acrolein, a highly reactive, unsaturated aldehyde and respiratory irritant, is formed during combustion (e.g., cigarette smoke), high-temperature cooking of foods, and in vivo as a product of oxidative stress and polyamine metabolism 3-Hydroxypropyl mercapturic acid (3-HPMA) is the main metabolite of acrolein in urine Previous studies demonstrated that betel quid can enhance the alkalinity of oral cavity, and might enhance the additional absorption of acrolein(pKa is about 9.0) However, this has not been shown in literature This study is designed to describe some characteristics of cigarette smokers in Kaohsiung and test the hypothesis that chewing betel quid will facilitate the absorption of acrolein while smoking cigarette by comparing the level of urinary 3-HPMA in cigarette-smokers with and without betel quid chewing Urine samples were obtained from healthy volunteers in Kaohsiung, which is located in the southern part of Taiwan Urinary 3-HPMA and creatinine were analyzed by LC-MS/MS and HPLC with UV detector, respectively The results showed that the level of 3-HPMA in cigarette-smokers who chewing betel quid (1.97 ng/ g creatinine) was significant higher (p