The present study was aimed to develop a most effective extender for long term storage of Beetal buck semen at 4℃. Tris-citric acid-fructose was supplemented with egg yolk (TEY), egg yolk plasma (TEYP), @ 5, 10, 15 and 20; 5, 10, 15 and 20 percent, respectively. Extended semen was stored at 4℃ and analyzed for sperm attributes.
Int.J.Curr.Microbiol.App.Sci (2020) 9(11): 1853-1864 International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume Number 11 (2020) Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2020.911.219 Effects of Egg Yolk and Egg Yolk Plasma Tris-Base Extenders on Beetal Buck Semen Preservation Bipanpreet Singh1*, Daljeet Kaur1, Mandeep Singla1, Ranjna S Cheema2, A.P.S Sethi3, Sumit Singhal4 and D.S Malik1 Department of Livestock Production Management, COVS, 2Department of Bioinfromatics, Department of Animal Nutrition, 4Directorate Livestock Farms, GADVASU, Ludhiana-141004, India *Corresponding author ABSTRACT Keywords Egg yolk, Plasma, Beetal buck semen preservation Article Info Accepted: 15 October 2020 Available Online: 10 November 2020 The present study was aimed to develop a most effective extender for long term storage of Beetal buck semen at 4℃ Tris-citric acid-fructose was supplemented with egg yolk (TEY), egg yolk plasma (TEYP), @ 5, 10, 15 and 20; 5, 10, 15 and 20 percent, respectively Extended semen was stored at 4℃ and analyzed for sperm attributes Values for motility, viability and plasma membrane integrity and acrosomal integrity remained significantly (p50% at 72 hrs of preservation Though, both types of extenders at 15% level of concentration were able to sustain sperm attributes to>60% level at 72 hrs of preservation Percent decline in sperm attributes was recorded comparatively less at 15 % concentration in both type of extenders Values for acrosome integrity also differed significantly (p