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B GIÁO D C VÀ ÀO T O TR NG I H C C N TH VI N NC & PT CÔNG NGH SINH H C LU N V N T T NGHI P I HOC NGÀNH CÔNG NGH SINH H C NGHIÊN C U TH Y PHÂN PROTEIN BÁNH D U U PH NG THÀNH M HÒA TAN B NG ENZYME PAPAIN, K T H P N M M C Aspergillus oryzae VÀ VI KHU N Bacillus subtilis CÁN B H NG D N ThS LÊ THANH HÙNG VI N NC&PT CNSH SINH VIÊN TH C HI N TR N NG C B O CHI MÃ S SINH VIÊN: 3064376 L P: CNSH K32 C n Th , Tháng 06/2010 PH N KÝ DUY T CÁN B H NG D N SINH VIÊN TH C HI N Lê Thanh Hùng Tr n Ng c B o Chi DUY T C A H I NG B O V LU N V N ………………………………………………………………………………………… ………………………………………………………………………………………… ………………………………………………………………………………………… ………………………………………………………………………………………… ………………………………………………………………………………………… C n Th , ngày tháng CH T CH H I n m 2010 NG L IC MT u tiên, xin kính dâng lòng bi t n d t n ba má ln bên c nh ng viên, khuy n khích su t th i gian h c t p Xin g i l i c m n sâu s c i u ki n thu n l i nh t, h l tơi hồn thành n Th y Lê Thanh Hùng ã t n tình giúp ng d!n truy n ,t om i t nh"ng kinh nghi m quý báu, khích tài lu n v#n Xin chân thành c m n cô Nguy$n Th% Xuân Mai - c v n h c t p l p Công ngh Sinh h c K32 ã quan tâm, trình h c t p t i tr ng viên t o i u ki n t t cho su t ng c&ng nh th i gian th'c hi n tài Xin chân thành c m n Th y Võ V#n Song Toàn, anh Qu , ch% Giang cán b phịng thí nghi m Vi n NC&PT Công ngh Sinh h c ã giúp , ch( b o nh"ng kinh nghi m b n thân, thao tác thí nghi m trình th'c hi n tài Xin c m n t p th) l p Công ngh Sinh h c K32 t t c b n bè ã c* v&, ng viên giúp su t th i gian qua Xin chân thành c m n v i t m lòng trân tr ng! C n Th , ngày 10 tháng 05 n#m 2010 Sinh viên th'c hi n Tr n Ng c B o Chi TÓM L C tài ”Nghiên c u th y phân protein bánh d u u ph ng thành m hịa tan b ng enzyme papain thơ, k t h p n m m c Aspergillus oryzae vi khu n Bacillus subtilis” c th c hi n v i m c ích tìm nh ng i u ki n t i u cho trình th y phân b ng protease vi sinh v t Thí nghi m hàm l c ti n hành nghiên c u nh h ng enzyme nh h ng ng c a y u t pH, nhi t , n trình th y phân b ng enzyme papain, protease n m m c Aspergillus oryzae vi khu n Bacillus subtilis Sau th i gian th c hi n tài, thu c m t s k t qu nh sau: - S d ng enzyme papain v i hàm l ph ng cho k t qu t t nh t ng 1,6%, sau gi th y phân bánh d u pH 5,5, nhi t u 60ºC - S d ng n m m c Aspergillus oryzae 2%, sau 20 ngày th y phân cho k t qu t t nh t pH 5,5, nhi t 45ºC v i hàm l ng 4,28g/l, m amoniac 0,49g/l Hi u su t t o thành acid amin 26,83% - Vi khu n Bacillus subtilis v i hàm l qu t t nh t 3,57g/l, pH 6,5, nhi t m t ng s 14,87g/l, m amin ng 2%, sau 20 ngày th y phân cho k t 50ºC v i hàm l ng m t ng 14,47g/l, m amin m amoniac 0,58g/l Hi u su t t o thành acid amin 24,67% - Khi s d ng n m m c Aspergillus oryzae 2% k t h p vi khu n Bacillus subtilis 2%, sau 20 ngày th y phân cho k t qu t t nh t m t ng 13,81g/l, m amin 3,19g/l, thành aicd amin 28,13% i pH 7,5, nhi t 50ºC v i hàm l ng m amoniac 0,47g/l Hi u su t t o M CL C PH+N KÝ DUY T L,I C-M T TÓM L C i M C L C ii DANH SÁCH B-NG v DANH SÁCH HÌNH vii CH /NG 0T V1N CH /NG L C KH-O TÀI LI U 2.1 Gi i thi u v u ph ng (Arachis hypogea) 2.1.1 Ngu n g c u ph ng 2.1.2 Giá tr c a u ph ng 2.2 Bánh d u u ph ng (BD P) 2.3 Enzyme protease 2.4 Cây u (Carica papaya L.) 2.4.1 Gi i thi u v u 2.4.2 Enzyme papain 2.5 N m m c Aspergillus oryzae 15 2.5.1 N m m c Aspergillus oryzae 15 2.5.2 Protease t n m m c Aspergillus oryzae 17 2.6 Vi khu4n Bacillus subtilis 17 2.6.1 Vi khu n Bacillus subtilis 17 2.6.2 Protease t vi khu n Bacillus subtilis 18 2.6.3 ng d ng c a vi khu n Bacillus subtilis 19 2.7 Quá trình th3y phân protein 20 CH NG PH NG TI N VÀ PH NG PHÁP 25 3.1 Ph ng ti n 25 3.1.1 Nguyên li u 25 3.1.2 Thi t b 25 3.1.3 Hóa ch t 26 3.2 Ph ng pháp thí nghi m 27 3.3 Thí nghi m 1: Kh o sát y u t nh h!"ng # n trình th$y phân bánh d u #%u ph&ng b'ng enzyme papain thô 27 3.3.1 Thí nghi m 1a: Kh o sát nh h ng c a pH n trình th y phân b ng enzyme papain thô 27 3.3.2 Thí nghi m 1b: Kh o sát nh h ng c a nhi t n trình th y phân b ng enzyme papain thô 28 ii 3.3.3 Thí nghi m 1c: Kh o sát nh h ng c a hàm l ng enzyme papain n trình th y phân b ng enzyme papain thô 29 3.3.4 Thí nghi m 1d: Kh o sát nh h ng c a th i gian n trình th y phân b ng enzyme papain thô 30 3.4 Thí nghi m 2: Kh o sát y u t nh h 5ng n trình th3y phân protein bánh d u u ph ng b6ng vi khu4n Bacillus subtilis n m m c Aspergillus oryzae31 CH NG K T QU VÀ TH O LU N 33 4.1 K t qu xác #(nh ho)t tính enzyme papain thơ, ho)t tính c$a protease n*m m c Aspergillus oryzae m%t s vi khu+n Bacillus subtilis 33 4.2 K t qu kh o sát y u t nh h!"ng # n trình th$y phân bánh d u #%u ph&ng b'ng enzyme papain thô 33 4.2.1 !nh h ng c a pH n hàm l ng m formol (g/l) sinh th y phân b ng enzyme papain thô 33 4.2.2 !nh h ng c a nhi t n hàm l ng m formol (g/l) sinh 34 4.2.3 !nh h ng c a hàm l ng papain thô n hàm l ng m formol (g/l) sinh 35 4.3 Các y u t nh h!"ng # n trình th$y phân protein bánh d u #%u ph&ng b'ng n*m m c Aspergillus oryzae 36 4.3.1 !nh h ng c a pH n trình th y phân protein bánh d u u ph ng b ng n m m c Aspergillus oryzae 37 4.3.2 !nh h ng c a nhi t n trình th y phân protein bánh d u u ph ng b ng n m m c Aspergillus oryzae 2% 40 4.3.3 !nh h ng t ng tác gi a nhi t pH n hàm l ng m amin b sung n m m c Aspergillus oryzae 2% 44 4.4 K t qu kh o sát y u t nh h!"ng # n trình th$y phân protein bánh d u #%u ph&ng b'ng vi khu+n Bacillus subtilis 2% 45 4.4.1 !nh h ng c a pH n n trình th y phân protein bánh d u u ph ng b ng vi khu n Bacillus subtilis 2% 45 4.4.2 !nh h ng c a nhi t n n trình th y phân protein bánh d u u ph ng b ng vi khu n Bacillus subtilis 2% 49 4.4.3 !nh h ng t ng tác gi a nhi t pH n hàm l ng m amin b sung vi khu n Bacillus subtilis 2% 52 4.5 Các y u t nh h!"ng # n trình th$y phân protein bánh d u #%u ph&ng b'ng n*m m c Aspergillus oryzae 2% k t h,p vi khu+n Bacillus subtilis 2% 54 4.5.1 !nh h ng c a pH n trình th y phân protein bánh d u u ph ng b ng n m m c Aspergillus oryzae 2% k t h p vi khu n Bacillus subtilis 2% 54 4.5.2 !nh h ng c a nhi t n trình th y phân protein bánh d u u ph ng b ng n m m c Aspergillus oryzae 2% k t h p vi khu n Bacillus subtilis 2% 57 4.5.3 !nh h ng t ng tác gi a nhi t pH n hàm l ng m amin b sung n m m c Aspergillus oryzae 2% k t h p vi khu n Bacillus subtilis 2% 61 CH NG K T LU N VÀ - NGH 63 5.1 K t lu%n 63 iii 5.2 ngh( 63 TÀI LI U THAM KH O 64 Ph/ ch! ng Các ph! ng pháp phân tích ki0m #(nh Ph/ ch! ng B ng s li u thơ c$a q trình th$y phân Ph/ ch! ng K t qu x1 lí th ng kê iv DANH SÁCH B NG B ng 1: -nh h 5ng c3a protease khác lên c ch t khác B ng 2: i u ki n sinh tr 5ng c3a n m Aspergillus oryzae 17 B ng 3: Thành ph n hóa h c c3a mu i 22 B ng 4: B trí thí nghi m kh o sát nh h 5ng c3a y u t pH n q trình th3y phân b6ng enzyme papain thơ 28 B ng 5: B trí thí nghi m kh o sát nh h 5ng c3a nhi t n trình th3y phân b6ng enzyme papain thơ 29 B ng 6: B trí thí nghi m kh o sát nh h 5ng c3a n7ng enzyme n trình th3y phân b6ng enzyme papain thô 30 B ng 7: B trí thí nghi m kh o sát nh h 5ng c3a n7ng enzyme n trình th3y phân b6ng enzyme papain thô 31 B ng 8: Thành ph n hóa h c c3a bánh d u B ng 9: Hàm l ng B ng 10: Hàm l ng B ng 11: Hàm l ng u ph ng 33 m formol (g/l) sinh d i tác m formol (g/l) sinh d ng c3a pH 33 i tác m formol (g/l) sinh d ng c3a nhi t i tác 35 ng c3a hàm l ng enzyme papain thô 36 B ng 12: Hàm l ng m formol (g/l) thay *i theo th i gian 37 B ng 13: -nh h 5ng c3a pH n hàm l ng m t*ng b* sung n m m c Aspergillus oryzae 2% 38 B ng 14: -nh h 5ng c3a pH n hàm l ng m amin b* sung n m m c Aspergillus oryzae 2% 39 B ng 15: -nh h 5ng c3a pH n hàm l ng m amoniac b* sung n m m c Aspergillus oryzae 2% 40 B ng 16: -nh h 5ng c3a nhi t n hàm l ng m t*ng b* sung n m m c Aspergillus oryzae 2% 41 B ng 17: -nh h 5ng c3a nhi t n hàm l ng m amin b* sung n m m c Aspergillus oryzae 2% 42 B ng 18: -nh h 5ng c3a nhi t n hàm l ng m amoniac b* sung n m m c Aspergillus oryzae 2% 44 v B ng 19: -nh h 5ng t ng tác gi"a pH nhi t n hàm l ng m amin (g/l) b* sung n m m c Aspergillus oryzae 2% 45 B ng 20: -nh h 5ng c3a pH n hàm l ng m t*ng b* sung vi khu4n Bacillus subtilis 2% 46 B ng 21: -nh h 5ng c3a pH n hàm l ng m amin b* sung vi khu4n Bacillus subtilis 2% 47 B ng 22: -nh h 5ng c3a pH n hàm l ng m amoniac b* sung vi khu4n Bacillus subtilis 2% 48 B ng 23: -nh h 5ng c3a nhi t n hàm l ng m t*ng b* sung vi khu4n Bacillus subtilis 2% 50 B ng 24: -nh h 5ng c3a nhi t n hàm l ng m amin b* sung vi khu4n Bacillus subtilis 2% 51 B ng 25: -nh h 5ng c3a nhi t n hàm l ng m amoniac b* sung vi khu4n Bacillus subtilis 2% 52 B ng 26: -nh h 5ng t ng tác gi"a pH nhi t n hàm l ng m amin (g/l) b* sung vi khu4n Bacillus subtilis 2% 53 B ng 27: -nh h 5ng c3a pH n hàm l ng m t*ng b* sung n m m c Aspergillus oryzae 2% k t h p vi khu4n Bacillus subtilis 2% 55 B ng 28: -nh h 5ng c3a pH n hàm l ng m amin b* sung n m m c Aspergillus oryzae 2% k t h p vi khu4n Bacillus subtilis 2% 56 B ng 29: -nh h 5ng c3a pH n hàm l ng m amoniac b* sung n m m c Aspergillus oryzae 2% k t h p vi khu4n Bacillus subtilis 2% 57 B ng 30: -nh h 5ng c3a nhi t n hàm l ng m t*ng b* sung n m m c Aspergillus oryzae 2% k t h p vi khu4n Bacillus subtilis 2% 58 B ng 31: -nh h 5ng c3a nhi t n hàm l ng m amin b* sung n m m c Aspergillus oryzae 2% k t h p vi khu4n Bacillus subtilis 2% 59 B ng 32: -nh h 5ng c3a nhi t n hàm l ng m amoniac b* sung n m m c Aspergillus oryzae 2% k t h p vi khu4n Bacillus subtilis 2% 60 B ng 33: -nh h 5ng t ng tác gi"a pH nhi t n hàm l ng m amin (g/l) b* sung n m m c Aspergillus oryzae 2% k t h p vi khu4n Bacillus subtilis 2% 62 vi DANH SÁCH HÌNH Hình 1: C u trúc tâm ho t ng c3a papain 11 Hình 2: N m m c Aspergillus oryzae 16 Hình 3: Vi khu4n Bacillus subtilis 18 Hình 4: Quy trình nghiên c8u th3y phân protein bánh d u u ph ng b6ng enzyme papain thô, k t h p n m m c Aspergillus oryzae vi khu4n Bacillus subtilis 28 Hình 5: Hàm l ng m formol (g/l) sinh d i tác ng c3a pH 34 Hình 6: Hàm l ng m formol (g/l) sinh d i tác ng c3a nhi t 35 Hình 7: Hàm l ng m formol (g/l) sinh d i tác ng c3a n7ng enzyme 36 Hình 8: S' bi n *i hàm l ng m formol (g/l) d Hình 9: S' bi n *i hàm l ng m t*ng (g/l) d Hình 10: S' bi n *i hàm l Hình 11: S' bi n *i hàm l ng ng i tác i tác m amin (g/l) d ng c3a th i gian 37 ng c3a pH theo th i gian 38 i tác ng c3a pH theo th i gian 39 m amoniac (g/l) d i tác ng c3a pH theo th i gian 40 Hình 12: S' bi n *i hàm l ng m t*ng (g/l) d i tác ng c3a nhi t theo th i gian 42 Hình 13: S' bi n *i hàm l ng m amin (g/l) d i tác ng c3a nhi t theo th i gian 43 Hình 14: S' bi n *i hàm l ng m amoniac (g/l) d i tác ng c3a nhi t theo th i gian 44 Hình 15: S' bi n *i hàm l ng m amin (g/l) d i tác ng c3a pH nhi t theo th i gian 45 Hình 16: S' bi n *i hàm l ng m t*ng (g/l) d i tác ng c3a pH theo th i gian 47 Hình 17: S' bi n *i hàm l ng m amin (g/l) d i tác ng c3a pH theo th i gian 48 Hình 18: S' bi n *i hàm l ng m amoniac (g/l) d i tác ng c3a pH theo th i gian 49 Hình 19: S' bi n *i hàm l ng m t*ng (g/l) d i tác ng c3a nhi t theo th i gian 50 Hình 20: S' bi n *i hàm l ng m amin (g/l) d i tác ng c3a nhi t theo th i gian 51 vii Ngày 20 Analysis of Variance for DAM AMIN - Type III Sums of Squares Source Sum of Squares Df Mean Square MAIN EFFECTS A:pH 0.722444 0.361222 B:Nhiet 6.30916 2.10305 INTERACTIONS AB 0.346573 0.0577622 RESIDUAL 0.023275 12 0.00193958 TOTAL (CORRECTED) 7.40145 23 All F-ratios are based on the residual mean square error Multiple Range Tests for DAM AMIN by pH F-Ratio P-Value 186.24 1084.28 0.0000 0.0000 29.78 0.0000 Method: 95.0 percent LSD pH Count LS Mean LS Sigma Homogeneous Groups X 5.5 2.98812 0.0155707 X 6.5 3.07125 0.0155707 X 7.5 3.39063 0.0155707 Multiple Range Tests for DAM AMIN by Nhiet Method: 95.0 percent LSD Nhiet Count LS Mean 2.415 2.96917 3.43583 3.78 2.7 -nh h 5ng t LS Sigma 0.0179795 0.0179795 0.0179795 0.0179795 Homogeneous Groups X X X X ng tác gi"a nhi t pH n hàm l ng m amoniac b* sung n m m c Aspergillus oryzae 2% Ngày Analysis of Variance for DAM AMONIAC - Type III Sums of Squares Source Sum of Squares Df Mean Square MAIN EFFECTS A:pH 0.0049 0.00245 B:Nhiet 0.000561458 0.000187153 INTERACTIONS AB 0.00326667 0.000544444 RESIDUAL 0.0079625 12 0.000663542 TOTAL (CORRECTED) 0.0166906 23 All F-ratios are based on the residual mean square error F-Ratio P-Value 3.69 0.28 0.0563 0.8374 0.82 0.5749 F-Ratio P-Value 2.11 3.96 0.1638 0.0355 1.07 0.4293 Multiple Range Tests for DAM AMONIAC by pH Method: 95.0 percent LSD pH Count LS Mean 5.5 0.284375 7.5 0.301875 6.5 0.319375 LS Sigma 0.00910729 0.00910729 0.00910729 Homogeneous Groups X XX X Multiple Range Tests for DAM AMONIAC by Nhiet Method: 95.0 percent LSD Nhiet Count LS Mean 0.2975 0.2975 0.303333 0.309167 LS Sigma 0.0105162 0.0105162 0.0105162 0.0105162 Homogeneous Groups X X X X Ngày Analysis of Variance for DAM AMONIAC - Type III Sums of Squares Source Sum of Squares Df Mean Square MAIN EFFECTS A:pH 0.00193958 0.000969792 B:Nhiet 0.00546146 0.00182049 INTERACTIONS AB 0.00296042 0.000493403 RESIDUAL 0.0055125 12 0.000459375 TOTAL (CORRECTED) 0.015874 23 All F-ratios are based on the residual mean square error Multiple Range Tests for DAM AMONIAC by pH Method: 95.0 percent LSD pH Count LS Mean 5.5 0.336875 7.5 0.35 6.5 0.35875 LS Sigma 0.00757772 0.00757772 0.00757772 Homogeneous Groups X X X Multiple Range Tests for DAM AMONIAC by Nhiet Method: 95.0 percent LSD Nhiet Count LS Mean 0.326667 0.344167 0.355833 0.3675 LS Sigma 0.00875 0.00875 0.00875 0.00875 Homogeneous Groups X XX X X Ngày 10 Analysis of Variance for DAM AMONIAC - Type III Sums of Squares Source Sum of Squares Df Mean Square MAIN EFFECTS A:pH 0.00193958 0.000969792 B:Nhiet 0.0140875 0.00469583 INTERACTIONS AB 0.0131688 0.00219479 RESIDUAL 0.0098 12 0.000816667 TOTAL (CORRECTED) 0.0389958 23 All F-ratios are based on the residual mean square error F-Ratio P-Value 1.19 5.75 0.3384 0.0113 2.69 0.0684 F-Ratio P-Value 37.00 75.95 0.0000 0.0000 1.95 0.1526 Multiple Range Tests for DAM AMONIAC by pH Method: 95.0 percent LSD pH Count LS Mean 5.5 0.35875 6.5 0.371875 7.5 0.380625 LS Sigma 0.0101036 0.0101036 0.0101036 Homogeneous Groups X X X Multiple Range Tests for DAM AMONIAC by Nhiet Method: 95.0 percent LSD Nhiet Count LS Mean 0.344167 0.355833 0.373333 0.408333 LS Sigma 0.0116667 0.0116667 0.0116667 0.0116667 Homogeneous Groups X X XX X Ngày 15 Analysis of Variance for DAM AMONIAC - Type III Sums of Squares Source Sum of Squares Df Mean Square MAIN EFFECTS A:pH 0.0264396 0.0132198 B:Nhiet 0.0814115 0.0271372 INTERACTIONS AB 0.00418542 0.000697569 RESIDUAL 0.0042875 12 0.000357292 TOTAL (CORRECTED) 0.116324 23 All F-ratios are based on the residual mean square error Multiple Range Tests for DAM AMONIAC by pH Method: 95.0 percent LSD pH Count LS Mean 6.5 0.380625 5.5 0.406875 7.5 0.450625 LS Sigma 0.0110102 0.0110102 0.0110102 Homogeneous Groups X X X Multiple Range Tests for DAM AMONIAC by Nhiet Method: 95.0 percent LSD Nhiet Count LS Mean 0.379167 0.385 0.431667 0.455 LS Sigma 0.0127135 0.0127135 0.0127135 0.0127135 Homogeneous Groups X X X X Ngày 20 Analysis of Variance for DAM AMONIAC - Type III Sums of Squares Source Sum of Squares Df Mean Square MAIN EFFECTS A:pH 0.0178646 0.00893229 B:Nhiet 0.0924365 0.0308122 INTERACTIONS AB 0.00786042 0.00131007 RESIDUAL 0.0055125 12 0.000459375 TOTAL (CORRECTED) 0.123674 23 All F-ratios are based on the residual mean square error F-Ratio P-Value 19.44 67.07 0.0002 0.0000 2.85 0.0578 Multiple Range Tests for DAM AMONIAC by pH Method: 95.0 percent LSD pH Count LS Mean 6.5 0.415625 5.5 0.42 7.5 0.494375 LS Sigma 0.0118476 0.0118476 0.0118476 Homogeneous Groups X X X Multiple Range Tests for DAM AMONIAC by Nhiet Method: 95.0 percent LSD Nhiet Count LS Mean 0.4025 0.42 0.466667 0.484167 2.8 -nh h 5ng t LS Sigma 0.0136804 0.0136804 0.0136804 0.0136804 Homogeneous Groups X X X X ng tác gi"a nhi t pH n hàm l ng m amoniac b* sung vi khu4n Bacillus subtilis 2% Ngày Analysis of Variance for DAM AMONIAC - Type III Sums of Squares Source Sum of Squares Df Mean Square MAIN EFFECTS A:pH 0.00500208 0.00250104 B:Nhiet 0.0006125 0.000204167 INTERACTIONS AB 0.00030625 0.0000510417 RESIDUAL 0.003675 12 0.00030625 TOTAL (CORRECTED) 0.00959583 23 All F-ratios are based on the residual mean square error F-Ratio P-Value 8.17 0.67 0.0058 0.5885 0.17 0.9810 Multiple Range Tests for DAM AMONIAC by pH Method: 95.0 percent LSD pH Count LS Mean 6.5 0.214375 5.5 0.2275 7.5 0.249375 LS Sigma 0.00618718 0.00618718 0.00618718 Homogeneous Groups X X X Multiple Range Tests for DAM AMONIAC by Nhiet Method: 95.0 percent LSD Nhiet Count LS Mean 0.2275 0.2275 0.2275 0.239167 LS Sigma 0.00714435 0.00714435 0.00714435 0.00714435 Homogeneous Groups X X X X Ngày Analysis of Variance for DAM AMONIAC - Type III Sums of Squares Source Sum of Squares Df Mean Square MAIN EFFECTS A:pH 0.0 0.0 B:Nhiet 0.00102083 0.000340278 INTERACTIONS AB 0.00694167 0.00115694 RESIDUAL 0.00245 12 0.000204167 TOTAL (CORRECTED) 0.0104125 23 All F-ratios are based on the residual mean square error F-Ratio P-Value 0.00 1.67 1.0000 0.2267 5.67 0.0053 Multiple Range Tests for DAM AMONIAC by pH Method: 95.0 percent LSD pH Count LS Mean 6.5 0.28875 7.5 0.28875 5.5 0.28875 LS Sigma 0.00505181 0.00505181 0.00505181 Homogeneous Groups X X X Multiple Range Tests for DAM AMONIAC by Nhiet Method: 95.0 percent LSD Nhiet Count LS Mean 0.28 0.285833 0.291667 0.2975 LS Sigma 0.00583333 0.00583333 0.00583333 0.00583333 Homogeneous Groups X X X X Ngày 10 Analysis of Variance for DAM AMONIAC - Type III Sums of Squares Source Sum of Squares Df Mean Square MAIN EFFECTS A:pH 0.0126583 0.00632917 B:Nhiet 0.0173031 0.00576771 INTERACTIONS AB 0.006125 0.00102083 RESIDUAL 0.0042875 12 0.000357292 TOTAL (CORRECTED) 0.040374 23 All F-ratios are based on the residual mean square error F-Ratio P-Value 17.71 16.14 0.0003 0.0002 2.86 0.0575 F-Ratio P-Value 21.00 8.38 0.0000 0.0053 10.38 0.0004 Multiple Range Tests for DAM AMONIAC by pH Method: 95.0 percent LSD pH Count LS Mean 5.5 0.319375 6.5 0.363125 7.5 0.371875 LS Sigma 0.00668292 0.00668292 0.00668292 Homogeneous Groups X X X Multiple Range Tests for DAM AMONIAC by Nhiet Method: 95.0 percent LSD Nhiet Count LS Mean 0.315 0.338333 0.3675 0.385 LS Sigma 0.00771677 0.00771677 0.00771677 0.00771677 Homogeneous Groups X X X X Ngày 15 Analysis of Variance for DAM AMONIAC - Type III Sums of Squares Source Sum of Squares Df Mean Square MAIN EFFECTS A:Nhiet 0.025725 0.008575 B:pH 0.00683958 0.00341979 INTERACTIONS AB 0.0254187 0.00423646 RESIDUAL 0.0049 12 0.000408333 TOTAL (CORRECTED) 0.0628833 23 All F-ratios are based on the residual mean square error Multiple Range Tests for DAM AMONIAC by pH Method: 95.0 percent LSD pH Count LS Mean 5.5 0.450625 7.5 0.459375 6.5 0.49 LS Sigma 0.00714435 0.00714435 0.00714435 Homogeneous Groups X X X Multiple Range Tests for DAM AMONIAC by Nhiet Method: 95.0 percent LSD Nhiet Count LS Mean 0.42 0.455 0.484167 0.5075 LS Sigma 0.00824958 0.00824958 0.00824958 0.00824958 Homogeneous Groups X X X X Ngày 20 Analysis of Variance for DAM AMONIAC - Type III Sums of Squares Source Sum of Squares Df Mean Square MAIN EFFECTS A:pH 0.0160271 0.00801354 B:Nhiet 0.165171 0.0550569 INTERACTIONS AB 0.00969792 0.00161632 RESIDUAL 0.00245 12 0.000204167 TOTAL (CORRECTED) 0.193346 23 All F-ratios are based on the residual mean square error F-Ratio P-Value 39.25 269.67 0.0000 0.0000 7.92 0.0013 Multiple Range Tests for DAM AMONIAC by pH Method: 95.0 percent LSD pH Count LS Mean 5.5 0.468125 6.5 0.520625 7.5 0.525 LS Sigma 0.00505181 0.00505181 0.00505181 Homogeneous Groups X X X Multiple Range Tests for DAM AMONIAC by Nhiet Method: 95.0 percent LSD Nhiet Count LS Mean 0.4025 0.443333 0.583333 0.589167 2.9 -nh h 5ng t LS Sigma 0.00583333 0.00583333 0.00583333 0.00583333 Homogeneous Groups X X X X ng tác gi"a nhi t pH n hàm l ng m amoniac b* sung n m m c Aspergillus oryzae 2% k t h p vi khu4n Bacillus subtilis 2% Ngày Analysis of Variance for DAM AMONIAC - Type III Sums of Squares Source Sum of Squares Df Mean Square MAIN EFFECTS A:pH 0.00438958 0.00219479 B:Nhiet 0.00178646 0.000595486 INTERACTIONS AB 0.00173542 0.000289236 RESIDUAL 0.0055125 12 0.000459375 TOTAL (CORRECTED) 0.013424 23 All F-ratios are based on the residual mean square error F-Ratio P-Value 4.78 1.30 0.0298 0.3206 0.63 0.7047 F-Ratio P-Value 15.36 1.79 0.0005 0.2030 0.70 0.6574 Multiple Range Tests for DAM AMONIAC by pH Method: 95.0 percent LSD pH Count LS Mean 6.5 0.266875 5.5 0.27125 7.5 0.2975 LS Sigma 0.00757772 0.00757772 0.00757772 Homogeneous Groups X X X Multiple Range Tests for DAM AMONIAC by Nhiet Method: 95.0 percent LSD Nhiet Count LS Mean 0.268333 0.274167 0.28 0.291667 LS Sigma 0.00875 0.00875 0.00875 0.00875 Homogeneous Groups X X X X Ngày Analysis of Variance for DAM AMONIAC - Type III Sums of Squares Source Sum of Squares Df Mean Square MAIN EFFECTS A:pH 0.0172521 0.00862604 B:Nhiet 0.00301146 0.00100382 INTERACTIONS AB 0.00234792 0.000391319 RESIDUAL 0.0067375 12 0.000561458 TOTAL (CORRECTED) 0.029349 23 All F-ratios are based on the residual mean square error Multiple Range Tests for DAM AMONIAC by pH Method: 95.0 percent LSD pH Count LS Mean 6.5 0.28875 5.5 0.28875 7.5 0.345625 LS Sigma 0.00837749 0.00837749 0.00837749 Homogeneous Groups X X X Multiple Range Tests for DAM AMONIAC by Nhiet Method: 95.0 percent LSD Nhiet Count LS Mean 0.291667 0.303333 0.315 0.320833 LS Sigma 0.00967349 0.00967349 0.00967349 0.00967349 Homogeneous Groups X X X X Ngày 10 Analysis of Variance for DAM AMONIAC - Type III Sums of Squares Source Sum of Squares Df Mean Square MAIN EFFECTS A:pH 0.0310333 0.0155167 B:Nhiet 0.00510417 0.00170139 INTERACTIONS AB 0.0114333 0.00190556 RESIDUAL 0.008575 12 0.000714583 TOTAL (CORRECTED) 0.0561458 23 All F-ratios are based on the residual mean square error F-Ratio P-Value 21.71 2.38 0.0001 0.1207 2.67 0.0699 F-Ratio P-Value 10.32 8.33 0.0025 0.0029 3.86 0.0222 Multiple Range Tests for DAM AMONIAC by pH Method: 95.0 percent LSD pH Count LS Mean 5.5 0.32375 6.5 0.35875 7.5 0.41125 LS Sigma 0.00945108 0.00945108 0.00945108 Homogeneous Groups X X X Multiple Range Tests for DAM AMONIAC by Nhiet Method: 95.0 percent LSD Nhiet Count LS Mean 0.344167 0.361667 0.3675 0.385 LS Sigma 0.0109132 0.0109132 0.0109132 0.0109132 Homogeneous Groups X XX XX X Ngày 15 Analysis of Variance for DAM AMONIAC - Type III Sums of Squares Source Sum of Squares Df Mean Square MAIN EFFECTS A:pH 0.0200083 0.0100042 B:Nhiet 0.0242448 0.0080816 INTERACTIONS AB 0.0224583 0.00374306 RESIDUAL 0.0116375 12 0.000969792 TOTAL (CORRECTED) 0.078349 23 All F-ratios are based on the residual mean square error Multiple Range Tests for DAM AMONIAC by pH Method: 95.0 percent LSD pH Count LS Mean 6.5 0.380625 5.5 0.406875 7.5 0.450625 LS Sigma 0.0110102 0.0110102 0.0110102 Homogeneous Groups X X X Multiple Range Tests for DAM AMONIAC by Nhiet Method: 95.0 percent LSD Nhiet Count LS Mean 0.379167 0.385 0.431667 0.455 LS Sigma 0.0127135 0.0127135 0.0127135 0.0127135 Homogeneous Groups X X X X Ngày 20 Analysis of Variance for DAM AMONIAC - Type III Sums of Squares Source Sum of Squares Df Mean Square MAIN EFFECTS A:pH 0.0313396 0.0156698 B:Nhiet 0.0265417 0.00884722 INTERACTIONS AB 0.0282771 0.00471285 RESIDUAL 0.013475 12 0.00112292 TOTAL (CORRECTED) 0.0996333 23 All F-ratios are based on the residual mean square error F-Ratio P-Value 13.95 7.88 0.0007 0.0036 4.20 0.0166 Multiple Range Tests for DAM AMONIAC by pH Method: 95.0 percent LSD pH Count LS Mean 6.5 0.415625 5.5 0.42 7.5 0.494375 LS Sigma 0.0118476 0.0118476 0.0118476 Homogeneous Groups X X X Multiple Range Tests for DAM AMONIAC by Nhiet Method: 95.0 percent LSD Nhiet Count LS Mean 0.4025 0.42 0.466667 0.484167 LS Sigma 0.0136804 0.0136804 0.0136804 0.0136804 Homogeneous Groups X X X X nh h!"ng t! ng tác gi;a pH nhi t #& # n hàm l!,ng #)m amin (g/l) " nghi m th3c b: sung vi sinh v%t Ngày ANOVA Table for DAM AMIN by Nghiem thuc Source Sum of Squares Df Mean Square Between groups 0.0469583 11 0.00426894 Within groups 0.033075 12 0.00275625 Total (Corr.) 0.0800333 23 F-Ratio 1.55 Table of Means for DAM AMIN by Nghiem thuc with 95.0 percent LSD intervals Stnd error Nghiem thuc Count Mean (pooled s) Lower limit Upper limit A1 5.5 1.2075 0.0371231 1.15031 1.26469 A1 6.5 1.155 0.0371231 1.09781 1.21219 A1 7.5 1.225 0.0371231 1.16781 1.28219 A2 5.5 1.2775 0.0371231 1.22031 1.33469 A2 6.5 1.26 0.0371231 1.20281 1.31719 A2 7.5 1.1725 0.0371231 1.11531 1.22969 A3 5.5 1.2075 0.0371231 1.15031 1.26469 A3 6.5 1.225 0.0371231 1.16781 1.28219 A3 7.5 1.1725 0.0371231 1.11531 1.22969 A4 5.5 1.26 0.0371231 1.20281 1.31719 A4 6.5 1.26 0.0371231 1.20281 1.31719 A4 7.5 1.1375 0.0371231 1.08031 1.19469 Total 24 1.21333 Multiple Range Tests for DAM AMIN by Nghiem thuc Method: 95.0 percent LSD Nghiem thuc Count A4 7.5 A1 6.5 A3 7.5 A2 7.5 A3 5.5 A1 5.5 A1 7.5 A3 6.5 A4 6.5 A4 5.5 A2 6.5 A2 5.5 Mean 1.1375 1.155 1.1725 1.1725 1.2075 1.2075 1.225 1.225 1.26 1.26 1.26 1.2775 Homogeneous Groups X XX XXX XXX XXX XXX XXX XXX XX XX XX X P-Value 0.2317 Ngày ANOVA Table for DAM AMIN by Nghiem thuc Source Sum of Squares Df Mean Square F-Ratio Between groups 5.15495 11 0.468632 33.63 Within groups 0.167213 12 0.0139344 Total (Corr.) 5.32217 23 Table of Means for DAM AMIN by Nghiem thuc with 95.0 percent LSD intervals Stnd error Nghiem thuc Count Mean (pooled s) Lower limit Upper limit A1 5.5 1.96 0.0834697 1.8314 2.0886 A1 6.5 1.96 0.0834697 1.8314 2.0886 A1 7.5 2.0475 0.0834697 1.9189 2.1761 A2 5.5 2.45 0.0834697 2.3214 2.5786 A2 6.5 2.4675 0.0834697 2.3389 2.5961 A2 7.5 2.5725 0.0834697 2.4439 2.7011 A3 5.5 3.08 0.0834697 2.9514 3.2086 A3 6.5 3.0975 0.0834697 2.9689 3.2261 A3 7.5 2.87 0.0834697 2.7414 2.9986 A4 5.5 3.43 0.0834697 3.3014 3.5586 A4 6.5 3.01 0.0834697 2.8814 3.1386 A4 7.5 2.5025 0.0834697 2.3739 2.6311 Total 24 2.62063 P-Value 0.0000 Multiple Range Tests for DAM AMIN by Nghiem thuc Method: 95.0 percent LSD Nghiem thuc Count A1 6.5 A1 5.5 A1 7.5 A2 5.5 A2 6.5 A4 7.5 A2 7.5 A3 7.5 A4 6.5 A3 5.5 A3 6.5 A4 5.5 Mean 1.96 1.96 2.0475 2.45 2.4675 2.5025 2.5725 2.87 3.01 3.08 3.0975 3.43 Homogeneous Groups X X X X X X X X X X X X Ngày 10 ANOVA Table for DAM AMIN by Nghiem thuc Source Sum of Squares Df Mean Square F-Ratio Between groups 3.58369 11 0.32579 80.80 Within groups 0.0483875 12 0.00403229 Total (Corr.) 3.63207 23 Table of Means for DAM AMIN by Nghiem thuc with 95.0 percent LSD intervals Stnd error Nghiem thuc Count Mean (pooled s) Lower limit Upper limit A1 5.5 2.2575 0.0449015 2.18832 2.32668 A1 6.5 2.415 0.0449015 2.34582 2.48418 A1 7.5 2.4325 0.0449015 2.36332 2.50168 A2 5.5 2.5375 0.0449015 2.46832 2.60668 A2 6.5 2.6425 0.0449015 2.57332 2.71168 A2 7.5 2.695 0.0449015 2.62582 2.76418 A3 5.5 3.22 0.0449015 3.15082 3.28918 A3 6.5 3.29 0.0449015 3.22082 3.35918 A3 7.5 2.9225 0.0449015 2.85332 2.99168 A4 5.5 3.5175 0.0449015 3.44832 3.58668 A4 6.5 3.115 0.0449015 3.04582 3.18418 A4 7.5 3.0975 0.0449015 3.02832 3.16668 Total 24 2.84521 Multiple Range Tests for DAM AMIN by Nghiem thuc Method: 95.0 percent LSD Nghiem thuc Count A1 5.5 A1 6.5 A1 7.5 A2 5.5 A2 6.5 A2 7.5 Mean 2.2575 2.415 2.4325 2.5375 2.6425 2.695 Homogeneous Groups X X X XX XX X P-Value 0.0000 A3 7.5 A4 7.5 A4 6.5 A3 5.5 A3 6.5 A4 5.5 2 2 2 2.9225 3.0975 3.115 3.22 3.29 3.5175 X X X XX X X Ngày 15 ANOVA Table for DAM AMIN by Nghiem thuc Source Sum of Squares Df Mean Square Between groups 7.75971 11 0.705428 Within groups 0.0312375 12 0.00260312 Total (Corr.) 7.79095 23 F-Ratio 270.99 P-Value 0.0000 Table of Means for DAM AMIN by Nghiem thuc with 95.0 percent LSD intervals Stnd error Nghiem thuc Count Mean (pooled s) Lower limit Upper limit A1 5.5 2.345 0.0360772 2.28942 2.40058 A1 6.5 2.45 0.0360772 2.39442 2.50558 A1 7.5 3.0625 0.0360772 3.00692 3.11808 A2 5.5 3.4475 0.0360772 3.39192 3.50308 A2 6.5 3.5175 0.0360772 3.46192 3.57308 A2 7.5 3.5875 0.0360772 3.53192 3.64308 A3 5.5 3.5875 0.0360772 3.53192 3.64308 A3 6.5 3.4825 0.0360772 3.42692 3.53808 A3 7.5 3.955 0.0360772 3.89942 4.01058 A4 5.5 4.2175 0.0360772 4.16192 4.27308 A4 6.5 4.06 0.0360772 4.00442 4.11558 A4 7.5 3.955 0.0360772 3.89942 4.01058 Total 24 3.47229 Multiple Range Tests for DAM AMIN by Nghiem thuc Method: 95.0 percent LSD Nghiem thuc Count A1 5.5 A1 6.5 A1 7.5 A2 5.5 A3 6.5 A2 6.5 A3 5.5 A2 7.5 A4 7.5 A3 7.5 A4 6.5 A4 5.5 Mean 2.345 2.45 3.0625 3.4475 3.4825 3.5175 3.5875 3.5875 3.955 3.955 4.06 4.2175 Homogeneous Groups X X X X XX XX X X X X X X Ngày 20 ANOVA Table for DAM AMIN by Nghiem thuc Source Sum of Squares Df Mean Square F-Ratio Between groups 4.32359 11 0.393053 308.03 Within groups 0.0153125 12 0.00127604 Total (Corr.) 4.3389 23 Table of Means for DAM AMIN by Nghiem thuc with 95.0 percent LSD intervals Stnd error Nghiem thuc Count Mean (pooled s) Lower limit Upper limit A1 5.5 3.0975 0.0252591 3.05858 3.13642 A1 6.5 3.1325 0.0252591 3.09358 3.17142 A1 7.5 3.325 0.0252591 3.28608 3.36392 A2 5.5 3.5 0.0252591 3.46108 3.53892 A2 6.5 3.5875 0.0252591 3.54858 3.62642 A2 7.5 3.78 0.0252591 3.74108 3.81892 A3 5.5 3.99 0.0252591 3.95108 4.02892 A3 6.5 4.27 0.0252591 4.23108 4.30892 A3 7.5 4.1825 0.0252591 4.14358 4.22142 A4 5.5 4.445 0.0252591 4.40608 4.48392 A4 6.5 3.4475 0.0252591 3.40858 3.48642 A4 7.5 3.57 0.0252591 3.53108 3.60892 Total 24 3.69396 Multiple Range Tests for DAM AMIN by Nghiem thuc Method: 95.0 percent LSD Nghiem thuc Count Mean Homogeneous Groups P-Value 0.0000 A1 5.5 A1 6.5 A1 7.5 A4 6.5 A2 5.5 A4 7.5 A2 6.5 A2 7.5 A3 5.5 A3 7.5 A3 6.5 A4 5.5 2 2 2 2 2 2 3.0975 3.1325 3.325 3.4475 3.5 3.57 3.5875 3.78 3.99 4.1825 4.27 4.445 X X X X XX XX X X X X X X Bacillus Ngày ANOVA Table for DAM AMIN by Nghiem thuc Source Sum of Squares Df Mean Square F-Ratio Between groups 0.0371583 11 0.00337803 3.68 Within groups 0.011025 12 0.00091875 Total (Corr.) 0.0481833 23 Table of Means for DAM AMIN by Nghiem thuc with 95.0 percent LSD intervals Stnd error Nghiem thuc Count Mean (pooled s) Lower limit Upper limit B1 5.5 1.3825 0.021433 1.34948 1.41552 B1 6.5 1.4 0.021433 1.36698 1.43302 B1 7.5 1.3825 0.021433 1.34948 1.41552 B2 5.5 1.4 0.021433 1.36698 1.43302 B2 6.5 1.4525 0.021433 1.41948 1.48552 B2 7.5 1.4525 0.021433 1.41948 1.48552 B3 5.5 1.435 0.021433 1.40198 1.46802 B3 6.5 1.47 0.021433 1.43698 1.50302 B3 7.5 1.47 0.021433 1.43698 1.50302 B4 5.5 1.505 0.021433 1.47198 1.53802 B4 6.5 1.4875 0.021433 1.45448 1.52052 B4 7.5 1.4525 0.021433 1.41948 1.48552 Total 24 1.44083 P-Value 0.0171 Multiple Range Tests for DAM AMIN by Nghiem thuc Method: 95.0 percent LSD Nghiem thuc Count B1 5.5 B1 7.5 B2 5.5 B1 6.5 B3 5.5 B4 7.5 B2 7.5 B2 6.5 B3 7.5 B3 6.5 B4 6.5 B4 5.5 Mean 1.3825 1.3825 1.4 1.4 1.435 1.4525 1.4525 1.4525 1.47 1.47 1.4875 1.505 Homogeneous Groups X X XX XX XXX XXX XXX XXX XX XX XX X Ngày ANOVA Table for DAM AMIN by Nghiem thuc Source Sum of Squares Df Mean Square Between groups 5.86652 11 0.53332 Within groups 0.018375 12 0.00153125 Total (Corr.) 5.8849 23 F-Ratio 348.29 Table of Means for DAM AMIN by Nghiem thuc with 95.0 percent LSD intervals Stnd error Nghiem thuc Count Mean (pooled s) Lower limit Upper limit B1 5.5 1.505 0.0276699 1.46237 1.54763 B1 6.5 1.645 0.0276699 1.60237 1.68763 B1 7.5 1.47 0.0276699 1.42737 1.51263 B2 5.5 1.6975 0.0276699 1.65487 1.74013 B2 6.5 1.6975 0.0276699 1.65487 1.74013 B2 7.5 1.96 0.0276699 1.91737 2.00263 B3 5.5 2.0475 0.0276699 2.00487 2.09013 B3 6.5 1.9075 0.0276699 1.86487 1.95013 B3 7.5 1.96 0.0276699 1.91737 2.00263 P-Value 0.0000 B4 5.5 B4 6.5 B4 7.5 Total 2 24 2.8875 2.9575 2.625 2.03 0.0276699 0.0276699 0.0276699 2.84487 2.91487 2.58237 2.93013 3.00013 2.66763 Multiple Range Tests for DAM AMIN by Nghiem thuc Method: 95.0 percent LSD Nghiem thuc Count B1 7.5 B1 5.5 B1 6.5 B2 6.5 B2 5.5 B3 6.5 B2 7.5 B3 7.5 B3 5.5 B4 7.5 B4 5.5 B4 6.5 Mean 1.47 1.505 1.645 1.6975 1.6975 1.9075 1.96 1.96 2.0475 2.625 2.8875 2.9575 Homogeneous Groups X X X X X X X X X X X X Ngày 10 ANOVA Table for DAM AMIN by Nghiem thuc Source Sum of Squares Df Mean Square Between groups 2.82419 11 0.256744 Within groups 0.0128625 12 0.00107187 Total (Corr.) 2.83705 23 F-Ratio 239.53 P-Value 0.0000 Table of Means for DAM AMIN by Nghiem thuc with 95.0 percent LSD intervals Stnd error Nghiem thuc Count Mean (pooled s) Lower limit Upper limit B1 5.5 1.855 0.0231503 1.81933 1.89067 B1 6.5 2.065 0.0231503 2.02933 2.10067 B1 7.5 1.9775 0.0231503 1.94183 2.01317 B2 5.5 2.03 0.0231503 1.99433 2.06567 B2 6.5 2.205 0.0231503 2.16933 2.24067 B2 7.5 2.4675 0.0231503 2.43183 2.50317 B3 5.5 2.5375 0.0231503 2.50183 2.57317 B3 6.5 2.6075 0.0231503 2.57183 2.64317 B3 7.5 2.3975 0.0231503 2.36183 2.43317 B4 5.5 2.905 0.0231503 2.86933 2.94067 B4 6.5 2.87 0.0231503 2.83433 2.90567 B4 7.5 2.73 0.0231503 2.69433 2.76567 Total 24 2.38729 Multiple Range Tests for DAM AMIN by Nghiem thuc Method: 95.0 percent LSD Nghiem thuc Count B1 5.5 B1 7.5 B2 5.5 B1 6.5 B2 6.5 B3 7.5 B2 7.5 B3 5.5 B3 6.5 B4 7.5 B4 6.5 B4 5.5 Mean 1.855 1.9775 2.03 2.065 2.205 2.3975 2.4675 2.5375 2.6075 2.73 2.87 2.905 Homogeneous Groups X X XX X X X XX XX X X X X Ngày 15 ANOVA Table for DAM AMIN by Nghiem thuc Source Sum of Squares Df Mean Square Between groups 3.54571 11 0.322337 Within groups 0.0079625 12 0.000663542 Total (Corr.) 3.55367 23 F-Ratio 485.78 Table of Means for DAM AMIN by Nghiem thuc with 95.0 percent LSD intervals Stnd error Nghiem thuc Count Mean (pooled s) Lower limit Upper limit P-Value 0.0000 B1 5.5 B1 6.5 B1 7.5 B2 5.5 B2 6.5 B2 7.5 B3 5.5 B3 6.5 B3 7.5 B4 5.5 B4 6.5 B4 7.5 Total 2 2 2 2 2 2 24 2.065 2.205 2.205 2.17 2.3975 2.485 2.8875 2.8875 2.8875 3.185 3.115 2.8875 2.61479 0.0182146 0.0182146 0.0182146 0.0182146 0.0182146 0.0182146 0.0182146 0.0182146 0.0182146 0.0182146 0.0182146 0.0182146 2.03694 2.17694 2.17694 2.14194 2.36944 2.45694 2.85944 2.85944 2.85944 3.15694 3.08694 2.85944 2.09306 2.23306 2.23306 2.19806 2.42556 2.51306 2.91556 2.91556 2.91556 3.21306 3.14306 2.91556 Multiple Range Tests for DAM AMIN by Nghiem thuc Method: 95.0 percent LSD Nghiem thuc Count B1 5.5 B2 5.5 B1 7.5 B1 6.5 B2 6.5 B2 7.5 B3 5.5 B4 7.5 B3 7.5 B3 6.5 B4 6.5 B4 5.5 Mean 2.065 2.17 2.205 2.205 2.3975 2.485 2.8875 2.8875 2.8875 2.8875 3.115 3.185 Homogeneous Groups X X X X X X X X X X X X Ngày 20 ANOVA Table for DAM AMIN by Nghiem thuc Source Sum of Squares Df Mean Square Between groups 4.94226 11 0.449297 Within groups 0.0098 12 0.000816667 Total (Corr.) 4.95206 23 F-Ratio 550.16 Table of Means for DAM AMIN by Nghiem thuc with 95.0 percent LSD intervals Stnd error Nghiem thuc Count Mean (pooled s) Lower limit Upper limit B1 5.5 2.205 0.0202073 2.17387 2.23613 B1 6.5 2.485 0.0202073 2.45387 2.51613 B1 7.5 2.38 0.0202073 2.34887 2.41113 B2 5.5 2.3975 0.0202073 2.36637 2.42863 B2 6.5 2.66 0.0202073 2.62887 2.69113 B2 7.5 2.73 0.0202073 2.69887 2.76113 B3 5.5 2.9575 0.0202073 2.92637 2.98863 B3 6.5 3.29 0.0202073 3.25887 3.32113 B3 7.5 3.1675 0.0202073 3.13637 3.19863 B4 5.5 3.605 0.0202073 3.57387 3.63613 B4 6.5 3.57 0.0202073 3.53887 3.60113 B4 7.5 3.0975 0.0202073 3.06637 3.12863 Total 24 2.87875 Multiple Range Tests for DAM AMIN by Nghiem thuc Method: 95.0 percent LSD Nghiem thuc Count B1 5.5 B1 7.5 B2 5.5 B1 6.5 B2 6.5 B2 7.5 B3 5.5 B4 7.5 B3 7.5 B3 6.5 B4 6.5 B4 5.5 Mean 2.205 2.38 2.3975 2.485 2.66 2.73 2.9575 3.0975 3.1675 3.29 3.57 3.605 Homogeneous Groups X X X X X X X X X X X X P-Value 0.0000 A+B Ngày ANOVA Table for DAM AMIN by Nghiem thuc Source Sum of Squares Df Mean Square Between groups 0.317887 11 0.0288989 Within groups 0.037975 12 0.00316458 Total (Corr.) 0.355863 23 F-Ratio 9.13 P-Value 0.0003 Table of Means for DAM AMIN by Nghiem thuc with 95.0 percent LSD intervals Stnd error Nghiem thuc Count Mean (pooled s) Lower limit Upper limit AB1 5.5 1.0675 0.039778 1.00622 1.12878 AB1 6.5 1.1375 0.039778 1.07622 1.19878 AB1 7.5 1.0325 0.039778 0.971216 1.09378 AB2 5.5 0.9625 0.039778 0.901216 1.02378 AB2 6.5 1.0325 0.039778 0.971216 1.09378 AB2 7.5 1.2425 0.039778 1.18122 1.30378 AB3 5.5 0.8225 0.039778 0.761216 0.883784 AB3 6.5 0.9625 0.039778 0.901216 1.02378 AB3 7.5 1.225 0.039778 1.16372 1.28628 AB4 5.5 1.015 0.039778 0.953716 1.07628 AB4 6.5 1.1725 0.039778 1.11122 1.23378 AB4 7.5 1.0325 0.039778 0.971216 1.09378 Total 24 1.05875 Multiple Range Tests for DAM AMIN by Nghiem thuc Method: 95.0 percent LSD Nghiem thuc Count AB3 5.5 AB3 6.5 AB2 5.5 AB4 5.5 AB1 7.5 AB4 7.5 AB2 6.5 AB1 5.5 AB1 6.5 AB4 6.5 AB3 7.5 AB2 7.5 Mean 0.8225 0.9625 0.9625 1.015 1.0325 1.0325 1.0325 1.0675 1.1375 1.1725 1.225 1.2425 Homogeneous Groups X X X XX XX XX XX XXX XXX XX X X Ngày ANOVA Table for DAM AMIN by Nghiem thuc Source Sum of Squares Df Mean Square Between groups 5.44737 11 0.495216 Within groups 1.27767 12 0.106473 Total (Corr.) 6.72505 23 F-Ratio 4.65 Table of Means for DAM AMIN by Nghiem thuc with 95.0 percent LSD intervals Stnd error Nghiem thuc Count Mean (pooled s) Lower limit Upper limit AB1 5.5 2.17 0.23073 1.81452 2.52548 AB1 6.5 1.82 0.23073 1.46452 2.17548 AB1 7.5 1.8725 0.23073 1.51702 2.22798 AB2 5.5 1.505 0.23073 1.14952 1.86048 AB2 6.5 1.7675 0.23073 1.41202 2.12298 AB2 7.5 2.135 0.23073 1.77952 2.49048 AB3 5.5 2.8 0.23073 2.44452 3.15548 AB3 6.5 2.66 0.23073 2.30452 3.01548 AB3 7.5 3.0625 0.23073 2.70702 3.41798 AB4 5.5 2.6075 0.23073 2.25202 2.96298 AB4 6.5 2.8175 0.23073 2.46202 3.17298 AB4 7.5 2.4675 0.23073 2.11202 2.82298 Total 24 2.30708 Multiple Range Tests for DAM AMIN by Nghiem thuc Method: 95.0 percent LSD Nghiem thuc Count AB2 5.5 AB2 6.5 Mean 1.505 1.7675 Homogeneous Groups X XX P-Value 0.0067 AB1 6.5 AB1 7.5 AB2 7.5 AB1 5.5 AB4 7.5 AB4 5.5 AB3 6.5 AB3 5.5 AB4 6.5 AB3 7.5 2 2 2 2 2 1.82 1.8725 2.135 2.17 2.4675 2.6075 2.66 2.8 2.8175 3.0625 XX XX XXX XXX XXX XX XX XX XX X Ngày 10 ANOVA Table for DAM AMIN by Nghiem thuc Source Sum of Squares Df Mean Square Between groups 4.43062 11 0.402784 Within groups 0.128625 12 0.0107187 Total (Corr.) 4.55925 23 F-Ratio 37.58 P-Value 0.0000 Table of Means for DAM AMIN by Nghiem thuc with 95.0 percent LSD intervals Stnd error Nghiem thuc Count Mean (pooled s) Lower limit Upper limit AB1 5.5 2.24 0.0732078 2.12721 2.35279 AB1 6.5 2.135 0.0732078 2.02221 2.24779 AB1 7.5 2.275 0.0732078 2.16221 2.38779 AB2 5.5 2.1875 0.0732078 2.07471 2.30029 AB2 6.5 2.3275 0.0732078 2.21471 2.44029 AB2 7.5 2.87 0.0732078 2.75721 2.98279 AB3 5.5 2.8525 0.0732078 2.73971 2.96529 AB3 6.5 3.08 0.0732078 2.96721 3.19279 AB3 7.5 3.185 0.0732078 3.07221 3.29779 AB4 5.5 3.3775 0.0732078 3.26471 3.49029 AB4 6.5 3.0275 0.0732078 2.91471 3.14029 AB4 7.5 3.0275 0.0732078 2.91471 3.14029 Total 24 2.71542 Multiple Range Tests for DAM AMIN by Nghiem thuc Method: 95.0 percent LSD Nghiem thuc Count AB1 6.5 AB2 5.5 AB1 5.5 AB1 7.5 AB2 6.5 AB3 5.5 AB2 7.5 AB4 7.5 AB4 6.5 AB3 6.5 AB3 7.5 AB4 5.5 Mean 2.135 2.1875 2.24 2.275 2.3275 2.8525 2.87 3.0275 3.0275 3.08 3.185 3.3775 Homogeneous Groups X X X X X X XX XXX XXX XX XX X Ngày 15 ANOVA Table for DAM AMIN by Nghiem thuc Source Sum of Squares Df Mean Square Between groups 4.28439 11 0.38949 Within groups 0.0140875 12 0.00117396 Total (Corr.) 4.29847 23 F-Ratio 331.77 Table of Means for DAM AMIN by Nghiem thuc with 95.0 percent LSD intervals Stnd error Nghiem thuc Count Mean (pooled s) Lower limit Upper limit AB1 5.5 2.2575 0.0242277 2.22017 2.29483 AB1 6.5 2.1875 0.0242277 2.15017 2.22483 AB1 7.5 2.31 0.0242277 2.27267 2.34733 AB2 5.5 2.31 0.0242277 2.27267 2.34733 AB2 6.5 2.555 0.0242277 2.51767 2.59233 AB2 7.5 2.8875 0.0242277 2.85017 2.92483 AB3 5.5 2.87 0.0242277 2.83267 2.90733 AB3 6.5 3.115 0.0242277 3.07767 3.15233 AB3 7.5 3.115 0.0242277 3.07767 3.15233 AB4 5.5 3.325 0.0242277 3.28767 3.36233 P-Value 0.0000 AB4 6.5 AB4 7.5 Total 2 24 3.29 3.255 2.78979 0.0242277 0.0242277 3.25267 3.21767 3.32733 3.29233 Multiple Range Tests for DAM AMIN by Nghiem thuc Method: 95.0 percent LSD Nghiem thuc Count AB1 6.5 AB1 5.5 AB2 5.5 AB1 7.5 AB2 6.5 AB3 5.5 AB2 7.5 AB3 7.5 AB3 6.5 AB4 7.5 AB4 6.5 AB4 5.5 Mean 2.1875 2.2575 2.31 2.31 2.555 2.87 2.8875 3.115 3.115 3.255 3.29 3.325 Homogeneous Groups X XX X X X X X X X X X X Ngày 20 ANOVA Table for DAM AMIN by Nghiem thuc Source Sum of Squares Df Mean Square Between groups 7.37818 11 0.670743 Within groups 0.023275 12 0.00193958 Total (Corr.) 7.40145 23 F-Ratio 345.82 Table of Means for DAM AMIN by Nghiem thuc with 95.0 percent LSD intervals Stnd error Nghiem thuc Count Mean (pooled s) Lower limit Upper limit AB1 5.5 2.3625 0.0311415 2.31452 2.41048 AB1 6.5 2.3625 0.0311415 2.31452 2.41048 AB1 7.5 2.52 0.0311415 2.47202 2.56798 AB2 5.5 2.6775 0.0311415 2.62952 2.72548 AB2 6.5 2.765 0.0311415 2.71702 2.81298 AB2 7.5 3.465 0.0311415 3.41702 3.51298 AB3 5.5 3.185 0.0311415 3.13702 3.23298 AB3 6.5 3.43 0.0311415 3.38202 3.47798 AB3 7.5 3.6925 0.0311415 3.64452 3.74048 AB4 5.5 3.7275 0.0311415 3.67952 3.77548 AB4 6.5 3.7275 0.0311415 3.67952 3.77548 AB4 7.5 3.885 0.0311415 3.83702 3.93298 Total 24 3.15 Multiple Range Tests for DAM AMIN by Nghiem thuc Method: 95.0 percent LSD Nghiem thuc Count AB1 6.5 AB1 5.5 AB1 7.5 AB2 5.5 AB2 6.5 AB3 5.5 AB3 6.5 AB2 7.5 AB3 7.5 AB4 6.5 AB4 5.5 AB4 7.5 Mean 2.3625 2.3625 2.52 2.6775 2.765 3.185 3.43 3.465 3.6925 3.7275 3.7275 3.885 Homogeneous Groups X X X X X X X X X X X X P-Value 0.0000 ... ? ?Nghiên c3u th$y phân protein bánh d u #%u ph&ng thành #)m hòa tan b'ng enzyme papain, k t h,p n*m m c Aspergillus oryzae vi khu+n Bacillus subtilis.” c th'c hi n v i b c h p cho trình th3y phân. .. thành c m n v i t m lòng trân tr ng! C n Th , ngày 10 tháng 05 n#m 2010 Sinh viên th'c hi n Tr n Ng c B o Chi TÓM L C tài ? ?Nghiên c u th y phân protein bánh d u u ph ng thành m hòa tan b ng enzyme. .. ch t, vào ng HCT b n v"ng c3a phân t enzyme nh h 5ng n s' k t h p gi"a ph n protein ph n khơng ph i protein (nhóm ph9 hay nhóm coenzyme) c3a enzyme Enzyme nh"ng protein v i nh"ng nhóm ;c bi t,
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