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The differential pulse voltammetric method at a hanging mercury drop electrode was used to determine the presence of ofloxacin. In this study, the conditions under which differential pulse voltammetric measurement could be conducted were examined and established.
oxacin in 0.3% (15 mg/5 mL) eye drops produced by the Traphaco Pharmaceutical Corporation Amount of ofloxacin in Conc Amount of compared to Sample Calculated result (µg/mL) ofloxacin/bottle manufacturer’s label (%) 2.837 14.185 94.57 X = 14.067 (93.80%) 2.768 13.840 92.27 SD = 0.20 2.7618 13.809 92.06 RSD = 1.43 % 2.8473 14.2365 94.91 m = 14.067 ± 0.210 2.8541 14.2705 95.14 (Confidence level 95%) 2.8154 14.077 93.85 No Table Amount of ofloxacin in 200 mg ofloxacin tablets produced by the Imexpharm Pharmaceutical Corporation Conc Amount of Percentage (%) Calculated data (µg/mL) ofloxacin/tablet 2.9548 196.987 98.49 X = 196.463 (98.23%) 3.024 201.600 100.80 SD = 3.33 2.9816 198.773 99.39 RSD = 1.69 % 2.9048 193.653 96.83 m = 196.463 ± 3.495 2.8924 192.827 96.41 (Confidence level 95%) 2.9241 194.940 97.47 The estimate of the amount of ofloxacin in the two drugs measured showed a relative standard deviation of < 2%, and a 2% deviation is allowed by the Vietnam Ministry of Health The percentage of ofloxacin calculated compared the level indicated on the label by the Traphaco Pharmaceutical Corporation is in the range of 92.06 95.14%; and its in the range 96.41 - 100.80% for the drugs produced by the Imexpharm Pharmaceutical Joint Stock Company These amounts are all within the standard presented in the Vietnamese Pharmacopoeia Conclusion In this study, optimal conditions for measuring ofloxacin using differential pulse voltammetry (DPV) techniques with hanging mercury drop electrode were successfully investigated The proposed method was also used to measure the amount of ofloxacin in samples of eye drop solution and antibiotics that are sold in Hanoi The results obtained from this study indicate the suitability of applying electrochemical methods to control medicine quality in the laboratory and pharmaceutical testing centers 83 Tran Quang Hai, Duong Quang Phung, Vu Thi Huong and Tu Vong Nghi REFERENCES [1] O Ballesteros, J.L Vílchez, A Navalón, 2002 Determination of the antibacterial ofloxacin in human urine and serum samples by solid-phase spectrofluorimetry Journal of Pharmaceutical and Biomedical Analysis 30, 1103 [2] N.E Basci, S Hanioglu-Kargi, H Soysal, A Bozkurt, S.O Kayaalp, 1997 Determination of ofloxacin in human aqueous humour by high-performance liquid chromatography with fluorescence detection Journal of Pharmaceutical and Biomedical Analysis 15, 663 [3] British Pharmacopoeia, 2008 Pp 1653-1654 [4] P.S Francis, J.L Adcock, 2005 Chemiluminescence methods for the determination of ofloxacin Analytica Chimica Acta 541, [5] H Hopkala, D Kowalczuk, 2000 Application of derivative UV spectrophotometry for the determination of ciprofloxacin, norfloxacin and ofloxacin in tablets Acta Poloniae Pharmaceutica 57, [6] C Horstkăotter, G Blaschke, 2011 Stereoselective determination of ofloxacin and its metabolites in human urine by capillary electrophoresis using laser-induced fluorescence detection Journal of Chromatography B 754, 169 [7] J Monk, D Campoli-Richards, 1987 ofloxacin: a review of its antibacterial activity, pharmacokinetic properties and therapeutic use Drugs 33,346 [8] Nelson, JM.; Chiller, TM.; Powers, JH.; Angulo, FJ., 2007 Fluoroquinolone-resistant Campylobacter species and the withdrawal of fluoroquinolones from use in poultry: a public health success story Clin Infect Dis 44, 977 [9] J Qiao, H Yan, H Wang, Y Lv, 2011 Determination of ofloxacin and lomefloxacin in chicken muscle using molecularly imprinted solid-phase extraction coupled with liquid chromatography Journal of Separation Science 34, 2668 [10] L da Silveira Ev, E.E.S Schapoval, 2002 Microbiological assay for determination of ofloxacin injection Journal of Pharmaceutical and Biomedical Analysis 27, 91 84 ... ciprofloxacin, norfloxacin and ofloxacin in tablets Acta Poloniae Pharmaceutica 57, [6] C Horstkăotter, G Blaschke, 2011 Stereoselective determination of ofloxacin and its metabolites in human urine... health success story Clin Infect Dis 44, 977 [9] J Qiao, H Yan, H Wang, Y Lv, 2011 Determination of ofloxacin and lomefloxacin in chicken muscle using molecularly imprinted solid-phase extraction... Chemiluminescence methods for the determination of ofloxacin Analytica Chimica Acta 541, [5] H Hopkala, D Kowalczuk, 2000 Application of derivative UV spectrophotometry for the determination of ciprofloxacin,