10/10/2020 Gram Stain Flashcards | Quizlet Scheduled maintenance: Saturday, October 10 from 4–5 PM PT On Saturday, October 10th, we'll be doing some maintenance on Quizlet to keep Dismiss things running smoothly Quizlet will be unavailable from 4-5 PM PT Gram Stain Leave the first rating STUDY Learn Flashcards Write Spell Test PLAY Match Created by sharedfile Key concepts: https://quizlet.com/428754843/gram-stain-flash-cards/ 1/9 10/10/2020 Gram Stain Flashcards | Quizlet Gram Positive Cells Have Gram Negative Cells Appear Gram Negative Cells Terms in this set (31) If you were staining all cells will stain purple Gram-positive bacilli and Gram-negative cocci, which of these micrographs would correctly represent the appearance of these cells after staining with crystal violet, rinsing, and blotting? After you add crystal Gram's iodine serves as a mordant Notice violet and rinse the slide, in the image below how it forms an what should be your insoluble complex with crystal violet This next step? helps the crystal violet remain in the grampositive cells during decolorization After Gram's iodine is The decolorizing step removes the crystal added and then rinsed, violet from the gram-negative cell walls, what is the next step in but not the gram-positive walls Watch the the Gram stain video to review the addition of procedure? decolorizing agent https://quizlet.com/428754843/gram-stain-flash-cards/ 2/9 10/10/2020 Gram Stain Flashcards | Quizlet What is the best Add decolorizing agent until run-off is procedure for clear decolorization? What happens to the The decolorizing agent dehydrates the Gram-positive cell wall peptidoglycan during decolorization? What happens to the The decolorizing agent dissolves the outer Gram-negative cell wall membrane during decolorization? Assuming the Gram stain The Gram-positive cells have thick walls, smear was blotted and and they will retain the purple crystal observed following the violet stain if decolorization was carried decolorization step, out correctly The Gram-negative cells will which micrograph be decolorized and are colorless Watch correctly illustrates how the video to review the effect of Gram-positive cocci and decolorization on both types of cells Gram-negative bacilli would appear? As bacterial cells age, Old Gram-positive cells will be their peptidoglycan decolorized begins to break apart What would be the effect on decolorization? How does safranin affect Safranin penetrates the cell wall, but not Gram-positive cells? enough of it is retained to cause a color change https://quizlet.com/428754843/gram-stain-flash-cards/ 3/9 10/10/2020 Gram Stain Flashcards | Quizlet If you FORGOT to the All would be purple decolorizing step, what colors would the Grampositive and Gramnegative cells be when viewed at the end of the procedure? You performed the Gram You skipped the safranin step stain on a smear You are expecting to find purple Gram-positive bacilli and pink Gram-negative cocci Instead, you observe pink bacilli and pink cocci Which of the following is NOT a possible explanation for this unusual result? You performed the Gram You forgot the safranin step stain You are expecting to find purple Grampositive cocci and pink Gram-negative bacilli Instead, you observe purple cocci but don't seem to see any bacilli How could you explain this? https://quizlet.com/428754843/gram-stain-flash-cards/ 4/9 10/10/2020 Gram Stain Flashcards | Quizlet Любые Светильники под проект Открыть svetmarket.ru Upgrade to remove ads Which of the following Only $2.99/month Escherichia coli organisms is gramnegative? Why is it recommended Over time, gram-positive cells lose their that Gram staining be ability to retain the crystal violet stain after performed on young decolorization bacterial cultures (not older than 24 hours)? What is the most critical The decolorization step step in the Gram staining procedure? If you forgot to add the colorless; purple counterstain to the mixed culture sample at the end of the staining procedure, gramnegative cells would appear and gram-positive cells would appear Which of the following Clusters of gram-positive cocci describes the correct cellular morphology and https://quizlet.com/428754843/gram-stain-flash-cards/ 5/9 10/10/2020 Gram Stain Flashcards | Quizlet Gram reaction for Staphylococcus aureus? Which of the following Gram-positive rods describes the correct cellular morphology and Gram reaction for Bacillus cereus? Why is it important that The contrast allows you to differentiate crystal violet be a between gram-negative and gram- contrasting color to positive cells by observing the color of safranin? the organism Which of the following Gram-negative cells carry a net negative statements is charge in their cell walls; gram-positive INCORRECT regarding cells carry a net positive charge the Gram staining procedure? The Gram stain is an differential example of a stain, because the process uses two contrasting stains to separate bacteria into groups based on cell wall composition While gram-positive peptidoglycan cells have a thick layer of in their cell walls, gram-negative cells have a thin layer, surrounded by an additional lipopolysaccharide layer https://quizlet.com/428754843/gram-stain-flash-cards/ 6/9 10/10/2020 Gram Stain Flashcards | Quizlet Which of the following Crystal violet and safranin basic stains is used in the Gram staining procedure? Which of the following Ethyl alcohol substances is used as a decolorizing agent in the Gram stain? If you forget to add the purple decolorizing agent when performing a Gram stain, what color will the gramnegative cells be? After performing a Gram pink or red; purple stain, gram-negative cells appear , while gram-positive cells appear What is the purpose of Gram's iodine is a killing agent, binds to using Gram's iodine crystal violet, and serves as a mordant during the Gram staining procedure? Which of the following Counterstain = Methylene blue represents an incorrect association regarding the reagents used during https://quizlet.com/428754843/gram-stain-flash-cards/ 7/9 10/10/2020 Gram Stain Flashcards | Quizlet the Gram Staining procedure? A fellow student is Without alcohol during the decolorizing unsure why his gram- step, the bacterial cell wall was not negative Escherichia coli altered, which prevented the Gram stain's is purple Upon iodine and crystal violet from being rinsed examining the Gram stain away kit, you notice it didn't have alcohol What caused the bacteria to be purple instead of pink? The Gram stain True differentiates between bacteria based on the thickness of their peptidoglycan layer (T/F) What is the difference The simple will only tell you between doing a simple morphological shape and size, while the stain versus a differential differential stain will allow for stain on a bacterial determination of size, shape, and type of slide? the cell wall OTHER SETS BY THIS CREATOR Micro 210 Lab Lecture exam 191 terms 227 terms sharedfile https://quizlet.com/428754843/gram-stain-flash-cards/ sharedfile 8/9 10/10/2020 sharedfile Gram Stain Flashcards | Quizlet sharedfile G210 Exam Microbio G210L Quiz 302 terms 40 terms sharedfile https://quizlet.com/428754843/gram-stain-flash-cards/ sharedfile 9/9 ... Micro 21 0 Lab Lecture exam 191 terms 22 7 terms sharedfile https://quizlet.com/ 428 754843/gram-stain-flash-cards/ sharedfile 8/9 10/10 /20 20 sharedfile Gram Stain Flashcards | Quizlet sharedfile G210... https://quizlet.com/ 428 754843/gram-stain-flash-cards/ 4/9 10/10 /20 20 Gram Stain Flashcards | Quizlet Любые Светильники под проект Открыть svetmarket.ru Upgrade to remove ads Which of the following Only $2. 99/month... review the addition of procedure? decolorizing agent https://quizlet.com/ 428 754843/gram-stain-flash-cards/ 2/ 9 10/10 /20 20 Gram Stain Flashcards | Quizlet What is the best Add decolorizing agent