Học viện Quân y Bộ môn Sinh lý bệnh Danh sách số cặp mồi (primers) xác định mầm bệnh VSV Hadong, 22/4/2008 Tên mồi fliCcom-s fliCd-as vi-s vi-as parat-s parat-as tyv-s tyv-as fliCcom-s fliCa-as Rfbj-s Rfbj-as FliCb-s FliCb-as rrn-s rrn-as VAI-F VAI-R VAG-F VAG-R cag5c-F Trình tự (5’ - 3’) AAT CAA CAA CAA CCT GCA GCG GCA TAG CCA CCA TCA ATA ACC GTT ATT TCA GCA TAA GGA G CTT CCA TAC CAC TTT CCG CTT GCT ATG GAA GAC ATA ACG AAC C CGT CTC CAT CAA AAG CTC CAT AGA GAG GAA GGG AAA TGA AGC TTT T TAG CAA ACT GTC TCC CAC CAT AC AAT CAA CAA CAA CCT GCA GCG TAG TGC TTA ATG TAG CCG AAG G CCA GCA CCA GTT CCA ACT TGA TAC GGC TTC CGG CTT TAT TGG TAA GCA TGT TAC ACC GAC GCC AGT TG AAC TTT AAG CGC GTA ACC CG GCA ACG CGA AGA ACC TTA CC GGT TAC CTT GTT ACG ACT T ATG GAA ATA CAA CAA ACA CAC CTG CTT GAA TGC GCC AAA C CAA TCT GTC CAA TCA AGC GAG GCG TCA AAA TAA TTC CAA GG GTT GAT AAC GCT GTC GCT TC cag3c-R GGG TTG TAT GAT ATT TTC CAT AA 23 SK145AB5 CCCACAAGATTTAAACTCC 19 Độ dài (bp) 21 21 19 18 25 24 22 23 21 22 24 24 20 20 20 19 21 19 21 20 20 Pathogen Sal H1:d Tên gen fliC Sal Vi viaB Sal O:2 prt Sal O:9 tyv Sal H1:a fliC Sal O:4 rfbJ Sal H1:b fliC Sal O:6 rrn HP vacA s1/vacA s2 (Atherton et al., 1995) vacA m1/vacA m2 (Atherton et al., 1999) cagA (Chattopadhyay et al., 2004) Yagyu F et al., 2002 HP HP HIV SK145AEG SK431A SK145 SK431 5’C2V3 3’V3 J5’-2KSI 3’C2V3 BECO5 BECO3 BE-ANCH B-SPEC E-SPEC b-act-F b-act-R tcpA-F tcpA-R CCCACAAGATTTAAATATGA TGAAGGGTACTAGTAGTTCC AGTGGGGGGACATCAAGCAGCCATGCAAAT TGCTATGTCAGTTCCCCTTGGTTCTCT TGTACACATGGAATTAGGCCAG ATGAATTCATTACAGTAGAAAAATTCCC ATAAGCTTGCAGTGTAGCAGAAGAAGA ATTTCTGGGTCCCCTCCTGAGG GGCATCAAACAGCTCCAGGCAAG AGCAAAGCCCTTTCTAAGCCCTGTCT TCCTGGCTGTGGAAAGATACCTA GTCCCCTCGGGGCTGGGAGG GTCTCAGTCCCTTGAGACTGCTG AGAGATGGCCACGGCTGCTT ATTTGCGGTGGACGATGGAG GAAGAAGTTTGTAAAAGAAGAACAC GAAAGCACCTTCTTTCACGTTG Cộng 20 20 30 27 22 28 27 22 24 26 23 20 23 20 20 25 22 885 bp HIV HIV HIV HIV HIV HIV Human V Cholera Chomvarin C et al., 2007 TS Nguyễn Lĩnh Toàn Chủ nhiệm Bộ môn Sinh lý bệnh HỌC VIỆN QUÂN Y