Evaluation of acute, sub-acute toxicity of Ich goi khang in the osteoarthritis mice model; evaluation the efficacity and the side effects of Ich goi khang in the primary osteoarthritis patients.
MINISTRY OF MINISTRY OF HEALTH EDUCATION AND TRAINING HA NOI MEDICAL UNIVERSITY NGUYEN THI BICH HONG ASSESSMENT TOXICITY AND EFFICACITY OF ICH GOI KHANG IN KNEE OSTEOARTHRITIS TREATMENT Specialized: Traditional medicine Code: 62720201 RESUME OF DOCTOR PHILOSOPHY THESIS HANOI – 2020 THE THESIS WAS COMPLETED IN HANOI MEDICAL UNIVERSITY Scientific Supervisor: Associate Professor Nguyen Thi Thu Ha Associate Professor Nguyen Tran Thi Giang Huong The first Reviewer: The second Reviewer: The third Reviewer: The Thesis approved by Council of scientists in the Hanoi Medical University At 2020 References theThesis in: Vietnam National Library Hanoi Medical University library THE RELATED PUBLICATION Nguyen Thi Bich Hong, Nguyen Thi Thu Ha, Nguyen Tran Thịi Giang Huong (2018) Experimental study of acute toxicity and hemodynamic effect of Ich goi khang treatment Vietnam Journal of Traditional Medicine, No 58, 33-41 Nguyen Thi Bich Hong, Nguyen Thi Thu Ha, Nguyen Tran Thi Giang Huong (2018) Assessment effect of Ich goi khang to the hepatic and renal function Vietnam Journal of Traditional Medicine, No 59, 13-23 Nguyen Thi Bich Hong, Nguyen Thi Thu Ha, Nguyen Tran Thi Giang Huong (2019) Assessment effect of pain relieving and ROM improvement by Ich Goi Khang therapy in patient with knee osteoarthritis, Vietnam Journal of Traditional Medicine, No 63, 13-23 INTRODUCTION Knee osteoarthritis is a chronic disease in which the most frequent pathology is articular cartilage, associated with the subchondral bone, ligament and synovium It is the common disease, the prevalence is 18 % among the female, and 9.5% among the male, and 15% population acquired this disease In Vietnam, osteoarthritis is ranged in the third common rheumatism diseases (4.66%), and 56.5% hosptitalised osteoarthritis patients was knee OA The mainstay treatment was NSAIDs The long-term usage of this medication can cause many side-effects Therefore, it is necessary to find out the natural medication which helps to reduce side-effects The combination medication “Ramulus cinnamomi and Prunus persica”, and “Semen coisis” which was added with others ingredients In traditional medicine, the Ramulus cinnamomi and Prunus persica ensure the circulation, Semen coisis helps to prevent the rheumatism diseases, beside, the chronic disease could lead to organ abnormalities In term of improve the treatment outcome of osteoarthritis, this thesis is conducted “Assessment the toxicity and the efficacity of “Ich goi khang” on the treatment of osteoarthritis” in order to: 1, Evaluation of acute, sub-acute toxicity of Ich goi khang in the osteoarthritis mice model 2, Evaluation the efficacity and the side effects of Ich goi khang in the primary osteoarthritis patients 1, THE SIGNIFICANCE OF THE THESIS Knee OA is the common disease in many countries around the world It leads to disability and handicap It affects not only health status but also patient economy In Vietnam, the treatment is parallel of modern and traditional medicine The orientation of the thesis was confirmation the efficacity and primary mechanism explanation in the OA model It is a pedal for the future study It suggests the natural treatment for OA 2, THE UP-TO-DATE OF THE THESIS Thesis evaluated the efficacity of new traditional medicine treatment It conducted an OA model by the usage of MIA (monosodium-iodacetate) which was first time introduced in Vietnam The thesis showed the scientific evidences to explain the anti-inflammatory and pain control effect, and promote the range motion The thesis concluded that: the Ich goi khang has no acute and sub-acute toxicity It also shows the anti-inflammatory and pain control effect in the animal model After 30 days of treatment, the VAS score reduced 2.88 1.32 points; the pain relieve and range motion recovery was reflected by the WOMAC score; the reduce of WOMAC score was 20.2 8.45 points The promotion of the knee function: heel-buttock reduced 6.45 5.65 cm; the productivity of average range motion 17.42 19.42 All of index was promoted in comparison with the control group (treated with Agelica laxiflora and Herba Taxilli), statistically significant p 0.05 p1-4; p34; p4-5 p1-2,3,4,5; p1-2,3,4,5 p2-3,4,5; p2-3,4,5; p3-5; p4- p3-4,5; p4-5 > 0.05 > 0.05 p3-4 < 0.05 p1-2,3,4,5; p2-3,4,5; p3-4,5; p4-5; > 0.05; p2-3 < 0.05 11 Slot n Force causing pain (g) After After After After After After Before w w w w w w p2-4,5 p2-5 < 0.05 < 0.01 < 0.01 p2-4 < 0.01 In the slots with IGK, after weeks of MIA injection, the force causing pain was in descending trend in comparison with model (p>0.05) - The pain relieve effect measured by Randall Selitto method Graph 3.2 Force causing pain in the knee according to time The result showed the IGK 17.04 g/kg slots need more force to cause the pain than the model slot, at the point after weeks (p