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An experimental study of bioethanol adsorption on natural Iranian clinoptilolite was carried out. Dynamic breakthrough curves were used to investigate the best adsorption conditions in bioethanol liquid phase. A laboratory setup was designed and fabricated for this purpose. In order to find the best operating conditions, the effect of liquid pressure, temperature and flow rate on breakthrough curves and consequently, maximum ethanol uptake by adsorbent were studied. The effects of different variables on final bioethanol concentration were investigated using Response Surface Methodology (RSM). The results showed that by working at optimum condition, feed with 96% (v/v) initial ethanol concentration could be purified up to 99.9% (v/v). In addition, the process was modeled using Box–Behnken model and optimum operational conditions to reach 99.9% for final ethanol concentration were found equal to 10.7 C, 4.9 bar and 8 mL/min for liquid temperature, pressure and flow rate, respectively. Therefore, the selected natural Iranian clinoptilolite was found to be a promising adsorbent material for bioethanol dehydration process.

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ORIGINAL ARTICLE

Experimental investigation of bioethanol liquid

phase dehydration using natural clinoptilolite

a

Biosystem Engineering Department, Tarbiat Modares University, Tehran 14115-336, Iran

bChemical Engineering Department, Tarbiat Modares University, Tehran 14115-143, Iran

c

Department of Chemical and Biomolecular Engineering, National University of Singapore (NUS), Singapore 117585, Singapore1

d

Department of Chemical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran 15875-4413, Iran

G R A P H I C A L A B S T R A C T

A R T I C L E I N F O

Article history:

Received 18 January 2016

Received in revised form 25 February

2016

A B S T R A C T

An experimental study of bioethanol adsorption on natural Iranian clinoptilolite was carried out Dynamic breakthrough curves were used to investigate the best adsorption conditions in bioethanol liquid phase A laboratory setup was designed and fabricated for this purpose In order to find the best operating conditions, the effect of liquid pressure, temperature and flow

* Corresponding author Tel.: +98 2148292308.

E-mail address: ghobadib@modares.ac.ir (B Ghobadian).

1 Current affiliation.

Peer review under responsibility of Cairo University.

Production and hosting by Elsevier

Cairo University Journal of Advanced Research

http://dx.doi.org/10.1016/j.jare.2016.02.009

2090-1232 Ó 2016 Production and hosting by Elsevier B.V on behalf of Cairo University.

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Accepted 29 February 2016

Available online 5 March 2016

Keywords:

Adsorption

Clinoptilolite

Dehydration

Bioethanol

Isotherm

Box–Behnken model

rate on breakthrough curves and consequently, maximum ethanol uptake by adsorbent were studied The effects of different variables on final bioethanol concentration were investigated using Response Surface Methodology (RSM) The results showed that by working at optimum condition, feed with 96% (v/v) initial ethanol concentration could be purified up to 99.9% (v/v).

In addition, the process was modeled using Box–Behnken model and optimum operational con-ditions to reach 99.9% for final ethanol concentration were found equal to 10.7 °C, 4.9 bar and

8 mL/min for liquid temperature, pressure and flow rate, respectively Therefore, the selected natural Iranian clinoptilolite was found to be a promising adsorbent material for bioethanol dehydration process.

Ó 2016 Production and hosting by Elsevier B.V on behalf of Cairo University.

Introduction

Fuel grade bioethanol is one of the widely used alternative for

fossil fuels or gasoline additive [1,2] In bioethanol–gasoline

mixture, the presence of even a very small amount of water

in bioethanol is unfavorable and leads to a two phase mixture

[3–5] The bioethanol dehydration is a process to eliminate

water from bioethanol–water mixture up to 99.6% (V/V)

There are several methods including azeotropic distillation

[6–8], extractive distillation [9,10], pervaporation with

mem-branes [11–13] and adsorption using adsorbents [3,5,14–18],

that are being used for water elimination to overcome the

ethanol–gasoline mixing problem The azeotropic distillation and extractive distillation are too expensive process[9,19] Lit-eratures show that extractive distillation is more complex due

to the design and process application and articles on energy consumption and cost, during recent years confirm that this method has high performance but needs further studies on energy consumption [20] Conventional extractive distillation

is energy consumption process because of using reboilers and condensors Different refine processes were used to improve conventional extractive distillation such as heat-pump-assisted extractive distillation for bioethanol purification[21], Ethanol dehydration via azeotropic distillation with gasoline fraction mixtures as entrainers [20]and Control comparison

of conventional and thermally coupled ternary extractive dis-tillation processes [22] In addition, although the pervapora-tion is a new generapervapora-tion in separapervapora-tion technology, it has industrial installation limitations The adsorption by selective porous adsorbents is a common high performance method in bioethanol dehydration Many studies have focused on differ-ent types of water adsorbdiffer-ents including biobased adsorbdiffer-ents namely natural corncobs, natural and activated palm stone and oak [3,23,24], Calcium Carbide [25], calcium chloride and lime[26], silica gel[27], cellulose and lignocellulose based (bleached wood pulp, oak sawdust and kenaf core) [14,28], Aluminas and c-alumina [29], Starch-Based Adsorbents [5,24,30]and different types of molecular sieves especially the zeolites [16,31–34] Finding appropriate, effective and cheap adsorbent material is a way to reduce the final bioethanol pro-duction costs The zeolites with porous structures and selectiv-ity properties can let water molecules to penetrate inside pore volumes of hydrophilic adsorbents and separate ethanol–water mixture The natural zeolites and clays such as clinoptilolite [17,35–37], chabazite and phillipsite[38]are plentiful material

in nature with hydrophilic properties suitable for ethanol–wa-ter separation For instance, it has been shown that the clinop-tilolite water adsorption capacity is more than 50% of water adsorption capacity of 3A zeolite [39] In various previous studies, the parameters influencing ethanol–water separation such as temperature[39], system pressure[40], adsorption heat [41]and particle size [39]have been investigated As a lot of industrial separation processes based on adsorption mecha-nism are carried out in liquid phase [42], using mesoporous adsorbents such as clinoptilolite is highly recommended to adsorb the big molecules in liquid phase[43] Although there are some studies on using clinoptilolite as a adsorbent for purification of ethanol in liquid phase [35,37], the effect of operational conditions has not well understood So, we aim

to use of Iranian clinoptilolite in both batch and continuous

Clinop-tilolite sample at T = 77 K (b) Pore size distribution based on

BJH method

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adsorption processes to separate the water contents from

water/ethanol mixture which is usual product of biofuel

pro-duction In this research work, the Iranian natural

clinoptilo-lite is presented as a cheap water adsorbent media to

separate the water from hydrous bioethanol in a fixed bed

setup Furthermore, the optimum operational conditions have

been found both experimentally and theoretically

Material and methods

The deionised water and ethanol were purchased from

Bide-stan Co (Qazvin-Iran) The natural clinoptilolite used in this

research work was purchased from Afrazand Co (East

Semnan–Iran) The chemical analysis showed the high content

of K+and Na+ The zeolite was approximately 65 wt.% pure

in clinoptilolite The composition of the material based on

X-ray fluorescence (XRF) (Model: Philips PW 2404) analysis

was 71.159 wt.% SiO2, 11.335 wt.% Al2O3, 0.936 wt.% Fe2O3,

0.807 wt.% CaO, 0.478 wt.% MgO, 3.064 wt.% Na2O,

4.48 wt.% K2O, 0.164 wt.% TiO2 and 0.847 wt.% SO3 Loss

of ignition (LOI) is 6.23 The bulk density was calculated and

it was found 820 kg m3(1–2 mm particle size) The silica

mod-ulus (molar ratio) of the sample wasg = SiO2/Al2O3= 6.26

The pore structure and surface area of Iranian clinoptilolite

were characterized by N2adsorption–desorption isotherm at

77 K which has been illustrated in Fig 1a Nitrogen

adsorp-tion was carried out using Belsorp mini II (Bel Japan) Before

the experiments, the sample was dried to be degassed at 25°C for 5 h and vacuum The adsorption isotherm has hysteresis loop along with a relative pressure from 0.4 to 0.99 This iso-therm is type I, which is typical property for mesoporous mate-rials[44] As it has been presented inTable 1, the BET surface area (aBET), total pore volume (Vt), (from the last point of iso-therm at a relative pressure of 0.99), micropore volume (Vm) and mean pore size have been calculated using Brunauer– Emmett–Teller (BET) method The Barrett–Joyner–Halenda (BJH) pore size distribution of the Iranian clinoptilolite sample was calculated based on the adsorption data As it can be seen

inFig 1b, the majority of pores have the radius size of less than 10 nm with mean pore diameter of 26.47 nm based on BJH method which has good agreement with what has been calculated from BET method[45](SeeTable 1)

A stainless steel column with 4 cm diameter and 55 cm height was designed and fabricated to regenerate samples using high temperature and vacuum pressure Regeneration column consists of three heating elements with a heating rate of approximately 20°C/min and indicators Three thermocouples provide the required feedback for an on/off temperature con-trolling system Vacuum gage is used for indicating the column vacuum pressure A cooling setup – condensers and cooling water circulator – collects regeneration liquid Afterward, regenerated zeolite is cooled to ambient temperature in desic-cator Regeneration operation is completed in 0.6 bar vacuum pressure and 300°C for 50 min

Static adsorption isotherms (batch)

Water removal from water/ethanol mixture by natural clinop-tilolite in batch condition was examined for different initial concentrations of water The experiments were carried out at ambient temperature (20°C) and static conditions in a thermo-stated laboratory scale adsorption vessel, with an

a BET (m 2 /g) V m (cm 3 /g) V t (cm 3 /g) D mean (nm) a BET (m 2 /g) V m (cm 3 /g) D mean (nm) 14.394 3.3071 0.094766 26.334 15.4 0.094327 26.334

bioethanol dehydration (1 Initial Container, 2 Liquid Pump, 3

Adsorption Column, 4 Cooling Circulator, 5 Temperature

Sensor, 6 Pressure Sensor, 7 Flowmeter and 8 Final Container.)

water adsorption

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initial liquid weight of 100 g The ethanol–water mixture at

dif-ferent concentrations was applied as adsorptive and 60 g

zeo-lite and the contact time of 24 h was selected for

experiments The water concentration in feed was varied

between 50 and 363 kg m3(kg of water in feed to feed

vol-ume) The Langmuir and Freundlich isotherm models were

used for description of the adsorption process (Eqs.(1) and

(2)):

qe¼kl Ce qm

where qe is the amount of solute adsorbed per unit weight of

solid (kmol/kg), Ce is equilibrium concentration of water

remaining in solution (kg/m3), qm is maximum adsorption

capacity (kmol/kg) and kfand klare Freundlich and Langmuir

constants (m3/kg), respectively 1/n is a measure of intensity of

adsorption The higher the 1/n value, the more favorable is the

adsorption qeis calculated from equation as follows (Eq.(3)):

qeðkmol=kgÞ¼C0Ce

Czeo

ð3Þ Because of using Temperature Swing Adsorption process

(TSA) for adsorbents regeneration (high temperature and

low pressure), it was necessary to find temperature dependent

isotherm An Extended Langmuir isotherm was used to find

adsorption dependence with temperature For this, qeand Ce

values in different temperature between 10 and 70°C were

found and temperature dependent equation was expressed as

follows:

qe¼ k1k2exp k3

T

 

Ce

1þ k2exp k3

T

 

Ce

ð4Þ

where k1is qm(kmol/kg), k2is kel(m3/kg) and k3isDH/R (°K)

DH and R are enthalpy changes and gas constant, respectively Dynamic adsorption (continuous)

An apparatus with packed bed adsorption column was designed for dynamic standard experiments The schematic

of the designed apparatus is shown inScheme 1 The column was designed based on the Yamamoto’s set-up dimensions for liquid phase adsorption [16] The retention time in the

Temperature (K) Uptake (kmol/kg) Langmuir Freundlich

k l (m 3 /kg) q m (kmol/kg) R 2 k f (kmol/kg) 1/n R 2

283 0.00746 0.0179 0.00746 0.986 0.00066 0.412 0.973

298 0.00701 0.0156 0.00701 0.986 0.00051 0.442 0.984

313 0.00689 0.0125 0.00689 0.991 0.00041 0.47 0.987

328 0.00662 0.0113 0.00662 0.971 0.00033 0.498 0.979

343 0.00628 0.0106 0.00629 0.974 0.000264 0.529 0.992

temperatures

(b) in different temperatures

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set-up was determined to be 21.2 min and for this research

work this value was assumed as 25 min According to the

max-imum flow rate of 14 mL/min, a stainless steel column with

4 cm diameter and 40 cm height was designed and fabricated

Its dimension ensured good flow distribution since the bed

internal diameter was at least 10 times as much as the particle

size and its length was at least 100 times as much as the particle size[40] A jacket of cooling water with 4 cm thickness was connected to circulator and surrounded the main column to fulfill the isotherm conditions Two pressure and temperature sensors were located on both inlet and outlet of the column for monitoring pressure drop and changes in system tempera-ture A copper coil was used in initial bioethanol container to control the initial bioethanol temperature.Scheme 1illustrates the experimental setup

Two pressure sensors in bottom and top of the column show the pressure and pressure drop Using circulator and temperature sensors, initial and final bioethanol temperatures were controlled The bioethanol concentration in initial tainer was constant and it was 96% The final bioethanol con-centration leaves the top valve and is shown by the Portable Density Meter of Anton DMA 35 A water flow meter (cali-brated for ethanol v/v concentration) was used for adjustment

of bioethanol flow rate and it is one of the main experimental parameters Before carrying out the experiments, the adsor-bent samples were treated by thermal regeneration for elimina-tion of water from the adsorbent pores The procedure was completed by putting samples in furnace for 2 h at 300°C and then it was cooled to ambient temperature in desiccator Natural zeolites with the HEU (Heulandite) framework are divided into two distinct classes based on Si/Al ratio Those with Si/Al of less than 4 are known as heulandite and those with Si/Al greater than 4 are known as clinoptilolite or silica-rich heulandite The key difference in these materials is those with Si/Al of less than 4 are not thermally stable to cal-cination above 350°C [46] and High silica Clinoptilolite is thermally stable to temperatures in excess of 500°C[47] The initial tank was filled with 96% (v/v) ethanol–water mixture The bioethanol entered from the bottom of the col-umn which was packed with a mass of natural zeolite When the mixture leaved the column, the pressure, temperature and flow rate were controlled The data collected and concentra-tion were obtained every minute by Density Meter All of the experiments were organized by RSM to find the optimal operational conditions This was done by Design-Expert 7 software

Results and discussion Static isotherm models

To determine the model to be used to describe the adsorption for an adsorbent–adsorbate isotherm experiments were carried out Initial concentration was varied from 50 to 363 kg m3for all the experiments at different temperature Raman spec-troscopy test was used to determine water–ethanol adsorption

constant T = 288 K and P = 3 bar, (b) different pressures and

constant F = 14 mL/min T = 293 K, and (c) different

tempera-tures and constant F = 10 mL/min and P = 1 bar

Coded factors Corresponding parameters Coded levels

Corresponding values

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on natural clinoptilolite The Raman spectra were collected at

the Spectroscopy Laboratory, Atomic and Molecular Group,

Physics Department, Tarbiat Modares University by using a

Thermo Nicolet Almega dispersive micro-Raman scattering

spectrometer Results showed that the main peak in water

Raman spectrum is for stretching O–H bond around 3000–

3400 cm1and it is obvious inFig 2that there is a strong peak

in this area after the treatment of zeolite by mixture of water and ethanol; hence, it could be concluded that only water is adsorbed and the amount of adsorbed ethanol is negligible

Pattern A (pressure) B (flow) C (temperature) Final concentration

response, and (c) effects of B and A on response

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By applying the linearization of both Langmuir and

Fre-undlich models, it has been indicated that data could be well

described by both models (Figs S1–S5 in Supplementary

infor-mation data) Linear form of experimental data for Langmuir

and Freundlich isotherms at different temperatures was shown

in Supplementary details (Figs S6–S15).Table 2shows

Lang-muir and Freundlich isotherm parameters in different

temper-atures varied between 283 and 343 K It is obvious that

Langmuir isotherm has a better correlation than Freundlich

at low temperatures near ambient Fortunately, working at

low temperature is desirable for industrial adsorption process

and based on data presented in Table 2, it is obvious that

decreasing in temperature causes increase in final uptake

Hence, Extended Langmuir isotherm was selected as a temper-ature dependent isotherm equation to describe the tempertemper-ature behavior in adsorption process that could also be used for any future process simulation or scale-up and design the industrial plant Eq (5) shows the Extended Langmuir model that describes the static data.Fig 3shows the experimental data and Extended Langmuir model at different temperatures Fur-thermore,Fig 4a and b shows the amount of uptake as a func-tion of temperature and initial concentrafunc-tion for both experimental data and Extended Langmuir model, respectively that are well matched together

qe¼0:0076  0:0001244exp 1386:5T

Ce

1þ 0:0001244exp 1386:5

T

Breakthrough curves based on dynamic study (Continuous)

In order to understand the effect of parameters including flow rate, pressure and temperature on the breakthrough curves and finding the optimum operating condition, the variation

of each parameter was studied The breakthrough curves in Fig 5show the effects of pressure, temperature and flow rate

on breakthrough point.Fig 5a shows that most of the mass transfer and adsorption takes place at the moment that the fluid first comes in contact with the inlet of the bed and by increasing the flow rate, there is a decrease in time required for saturation of adsorbent and breakthrough point shifts to the right side by increasing the flow rate Increasing pressure from 1 to 5 bar sat defined temperature (T = 293 K) also enhances the saturation time of adsorbent while there is a slight increase in adsorption capacity (Fig 5b) Reducing the temperature changes breakthrough point and increases

Source Sum of squares df Mean square F value P-value Prob > F

A-pressure 1.45 1 1.45 85.50 <0.0001

B-flow rate 0.080 1 0.080 4.73 0.0486

C-temperature 0.32 1 0.32 18.93 0.0008

Residual 0.22 13 0.017

Lack of fit 0.20 9 0.022 4.44 0.0826

Source Sum of squares df Mean square F value P-value Prob > F

A-pressure 1.45 1 1.45 119.00 <0.0001

B-flow rate 0.080 1 0.080 6.59 0.0372

C-temperature 0.32 1 0.32 26.35 0.0013

B2 2.632E 3 1 2.632E 3 0.22 0.6557

C2 2.632E 3 1 2.632E 3 0.22 0.6557

Residual 0.085 7 0.012

Lack of fit 0.065 3 0.022 4.33 0.0953

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adsorption, the breakthrough point shifts to the right side and

the maximum adsorption is obtained

To find the total adsorption capacity (q) from

break-through curve, the area above the curve divided into the total

area (above and under the curve) results in adsorption

percent-age[16] Hence, according to Eq.(6), total adsorption capacity

could be calculated from feed loading and adsorption

percentage

q¼Fqf

w C0

Z tb

0

1Ct

C0

The total adsorption capacity q inFig 5a for flow rates 6,

10 and 14 mL/min (in 3 bar pressure and 288 K) was obtained

1.75, 1.52 and 1.26 mol/kgzeo, respectively Also inFig 5b, for

column pressures 1, 3 and 5 bar (14 mL/min and 293 K), q

val-ues were calculated 1.5, 1.74 and 1.97 mol/kgzeo respectively

According to Fig 5c, for temperatures 283, 288 and 293 K

and 10 mL/min flow rate and 1 bar pressure, q values obtained

were 1.13, 1.31 and 1.44 mol/kgzeo, respectively

The response surface methodology

The response surface methodology (RSM) is a collection of

statistical and mathematical techniques for obtaining empirical

models Understanding the effect of parameters and

optimiza-tion is the common advantage of the RSM applicaoptimiza-tion.Table 3

shows the coded level for each parameter For the three parameters, the Box–Behnken Method was used In this method, each factor or independent variable is placed at one

of the three equally spaced values, usually coded as 1, 0 and +1 In this research work, the temperature, pressure and flow rate are independent variables and the final bioetha-nol concentration is the responses.Table 4shows the experi-mental design used in this study

The two linear and Quadratic models were used for the con-centration modeling in Box–Behnken method.Fig 6illustrates the different variables to response diagrams in linear model for concentration response An analysis of variance for linear model is shown inTable 5 The Model F-value of 36.39 implies the model significant The values of Prob > F less than 0.05 indicate that the model terms are significant and the parameter has a significant effect on the response In this case, A (pres-sure), B (flowrate) and C (temperature) are significant model terms The values greater than 0.10 indicate that the model terms are not significant The prediction expression is given

as follows:

Concentration¼ 99:43 þ 0:2125  P  0:025  F  0:04  T

ð7Þ According to Fig 6 increasing the pressure causes an increase in maximum final bioethanol concentration For the low temperatures, the effect of pressure on maximum

response and (c) effects of B and A on response

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bioethanol final concentration was intensified It can be seen

that there is a positive relation between the pressure and

max-imum bioethanol final concentration for a given temperature

Decreasing the temperature causes the maximum bioethanol

concentration to increase Reducing the temperature in liquid

affects the adsorbent surface and makes water molecules enter

the adsorbent pores The temperature control in liquid phase

adsorption process is more effective and it is simpler than

the other two operational parameters and the obtained results

show that the temperature has more influence on bioethanol

final concentration The flow rate decrease causes an increase

in maximum bioethanol concentration Reducing the flow rate

makes more retention time and hence creates a better contact

between the adsorbent and solute

Fig 7 shows the relationship between the predicted and

actual data line The R2value of 0.89 indicated that the actual

and the predicted data had a relatively good correlation in

lin-ear model

Analysis of variance for quadratic model is shown in

Table 6 The Model F-value of 18.11 implies that the model

is significant The values of Prob > F less than 0.05 indicate

that the model terms are significant and have a significant

effect on the response In this case, A (temperature), B

(flow-rate) and C (Temperature) are significant model terms The

values greater than 0.1 indicate that the model terms are not

significant The prediction expression in quadratic model is

given as follows (Eq.(8)):

Conc¼ 99:637 þ 0:3875  P  0:050  F  0:0750  T

 0:00625  PF þ 0:005  PT þ 0:005  FT

 0:03125P2 0:00015F2 0:001T2 ð8Þ

Fig 8 shows the independent variables to response

dia-grams in quadratic model Fig S16 illustrates the relationship

between the predicted and actual data line The R2 value of

0.92 shows a relatively good correlation between predicted

and actual data The optimization as a point of view of

con-centration shows that at 10.7°C liquid temperature, 4.9 bar

pressure and 8 mL/min liquid flow rate, the best response

was 99.9% bioethanol final concentrations

Conclusions

In this research work, Iranian natural clinoptilolite was used to

dehydrate hydrous ethanol Results showed that in optimum

operating conditions, bioethanol final concentration can reach

to 99.9% and above Static and dynamic studies were done and

adsorption isotherms were obtained and experimental data

were well described by Extended-Langmuir isotherm The

effects of operating parameters such as temperature, pressure

and flow rate were investigated on final ethanol concentration

and the adsorption process was optimized In Box–Behnken

analysis, the linear and quadratic models could be successfully

applied for description of dynamic process Results showed

that the selected natural clinoptilolite could be used as a

favor-able adsorbent in bioethanol drying without any pretreatment

processes

Conflict of Interests

The authors have declared no conflict of interests

Compliance with Ethics Requirements This article does not contain any studies with human or animal subjects

Acknowledgment The authors express their sincere thanks to Iranian Fuel Con-servation Company (IFCO) for the support during the course

of this research work

Appendix A Supplementary material

Supplementary data associated with this article can be found,

in the online version, athttp://dx.doi.org/10.1016/j.jare.2016 02.009

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