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Summary of biological doctoral thesis: Study on the diversity of leaf beetle (Chrysomelidae) and relationship with plant in environmental condition at Nui Chua national park, Ninh Thuan

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Objects of research: Appraising of the species diversity and the variation according to space of Chrysomelidae in Nui Chua national park. Determining the food of Chrysomelidae and appraising the change of their food according to space.

MINISTRY OF VIETNAM ACADEMY EDUCATION AND OF SCIENCE AND TRAINING TECHNOLOGY GRADUATE UNIVERSITY SCIENCE AND TECHNOLOGY -Nguyen Thi Dinh STUDY ON DIVERSITY OF LEAF BEETLE (CHRYSOMELIDAE) AND RELATIONSHIP WITH PLANT IN ENVIRONMENTAL CONDITION AT NÚI CHÚA NATIONAL PARK, NINH THUAN PROVINCE, VIETNAM BY MOLECULAR BOLOGICAL TOOL Major: Ecology Code: 42 01 20 SUMMARY OF BIOLOGICAL DOCTORAL THESIS Ha Noi - 2018 This dissertation was completed at Graduate University Science and Technology – Vietnam Academy of Science and Technology The first supervisor : Asso Prof Dr Nguyen Van Sinh The second supervisor: Dr Jesús Gómez-Zurita The first reviewer: … The second reviewer: … The thirt reviewer: … This dissertation will be defended before the academic evaluation board of the doctoral thesis, at meeting room in Graduate University Science and Technology – Vietnam Academy of Science and Technology, in h’, date … 2018… Please reference in: - Library of Graduate University Science and Technology - Vietnam national library INTRODUCTION Reason for choosing the topic Biomes are interdependent Plant communities relate to physical properties of environment as: temperature, humidity, solar radiation… Those physical properties change with altitude and vary the structure of plant communities Plant are food of insects and have an important role in the diversity and distribution of insects Study on responding of herbivore with the change of plant communities in altitude will help us over the understand about relationship interdependent each other in the ecosystem Chrysomelidae is the biggest family in Coleoptera order Their food are plant, therefore Chrysomelidae relates to plant in all their life and sampling them is easy For those reasons, Chrysomelidae is a suitable object to study the interaction of biomes in the ecosystem Nui Chua national park includes the area of semi-driest forest in Vietnam Plant communities change in altitude from dry forest on low land through semi-humid forest green humid forest on a high mountain For those reasons, Nui Chua national park is the ideal site to carry out researching For above all reason, I choose the topic “ Study on the diversity of leaf beetle (Chrysomelidae) and relationship with plant in environmental condition at Nui Chua national park, Ninh Thuan province, Vietnam by molecular biological tool ” Objects of research Appraising of the species diversity and the variation according to space of Chrysomelidae in Nui Chua national park 2 Determining the food of Chrysomelidae and appraising the change of their food according to space The main contents of the dissertation Using ADN Barcoding to appraise the species diversity of Chrysomelidae in Nui Chua national park Using AND Barcoding to determine the food of Chrysomelidae in Nui Chua national park Determine the component variables of Chrysomelidae and their food according to space in Nui Chua national park 1.1 CHAPTER OVERVIEW The topic relates to the dissertation 1.1.1 Using the molecular biological tool to measure biodiversity 1.1.1.1 Biodiversity and problems related Definement biodiversity: Biodiversity is the diversity in three levels: gene, species and ecosystem In there, the diversity of species is used in studying biology, conservation and ecology Mora et al (2011) predicted that there are about 8,7 million (± 1,3 million) of eukaryote on the earth; among there, about 2,2 million (± 0,18 million) live under the ocean And they think that about 86% of living species on the land and 91% of living species under ocean are waiting for describing So far, total known species in the world are approximately 1.900.000 species and every year there are 18.000 new species to be described (Chapman 2009) In there, tropical forest supports 50% of biodiversity on the earth (Myers 1988, Mittermeier et al 1998) and 80% of known insect species (WWF), 1998) although the tropical forest only covers

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