DSpace at VNU: Study on Isozymes from Aedes Aegypti Strains of some Residential Areas in Vietnam

V NU JO U R N A L F S C IE N C E N at S c i & Tech T XIX N.,4 2003 STUDY ON ISOZYM ES F R O M A ED ES A E G Y P T I S T R A IN S O F S O M E R E S ID E N T IA L A R E A S IN V IE T N A M D o N g o e L ie n , N g u y e n T h i T h u H o a i N a tio n a l Ư niưersity o f H a n o i V u Duc H uong In s titu te o f m a la ria , p a s ito lo g y a n d entom ology o f H a n o i A b stra ct lsozymes from vvilcỉ- caught collections of Aedes aegypti mơsquito were compared bv using polyacrvlamide gel electrophoresis These collections vvere collected from residential areas of Vietnam (North, South, Centre and Central highland area) This study revealed that there is an obvious relation between the insecticide resistance and the expression of isozyme esterase in the mosquito Some differences of isozymes between larvae and adults vvere also found Key ivords: D erìgue fever, in se cticid e re sista n ce , s tra in , Aedes a e g y p ti%PAG E, isozyme In tr o d u c tio n Dengue/ Dengue h e m o rrh a g ic fever (D F /D H F ) occurs th ro u g h o u t th e ye a r b u t p a rtic u la rly in su m m e r tim e (u s u a lly from June to J u ly ) I t e a s ily becomes epidem ic disease and tends to increase in S outhern region an d C e n tra l h ig h la n d o f V ie tn a m re ce n tly One reason fo r th is s itu a tio n is the in se cticid e resistance in dengue v iru s vectors in w hich Aedes ae gypti is an im p o rta n t one The m o squito has resisted to some insecticides such as D D T hexachloride benzen (o rg a n o c h lo rin e group ) and even p y re th ro id group w h ic h has been considered as a p o w e rfu l g ro u p is now losing its effect in some areas o f S o u th e rn region and C e n tra l h ig h la n d o f V ie tn a m [1, 2, 7] T h e re vvere some dom estic and foreign researches [1 , , 4, 7] s tu d y in g on the expression o f isozym e com plex in d iffe re n t Aedes a e g y p ti s tra in s in o rd e r to c x p la in th e re sista n ce a b ility in m o sq u ito H ow ever, some o p in io n s are c o n tro v e rs ia l The ob jective o f th is research is to fin d o u t th e isozym e m a rk e r fo r in se cticid e resistance by m eans o f s tu d v on th e e le c tro p h o re tic e x p re ssio n o f seve ral isozvmos: E sterase (EST), O cta n o l dehydrogenase (O D H ), M a la te dehydroge nase (M D H ), A cid phosphatase (ACP), A lk a lin e phosphatase (A LP ) F rom th is s tu d y , people can have a m ore a p p ro p ria te m e asurc to choose in se cticid e s fo r each re s id e n tia l area T h ere íbre , we have done these w orks: - A n a lv z in g th e e le c tro p h o re tic re s u lts on p o ly a c ry la m id e gel o f isozym es fro m d iffe re n t g e o g p h ica l Aedes ae gvpti s tra in s in V ie tn a m 28 S t u d x on is m y m c s f n n t i (irt/rs a v ỊỊx p ỉi s t r a in s of 29 C o m p íin n ^ the* r l r r t r o p h n r r tir rx p re s s io n o f r s t r r a s r ỉro m larvíU ' and a d u lts b\ te e h n iq u e ol |> n lv;irrv|;im i< ir n rl rlo c tro p h o re s is (Ỉ\*\(ÌK )